UniProtKB - Q8R4Z9 (MFN1_RAT)
Mitofusin-1
Mfn1
Functioni
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 284 | GTPBy similarity | 1 | |
Binding sitei | 286 | GTPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 85 – 90 | GTPBy similarity | 6 | |
Nucleotide bindingi | 237 – 240 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB-KW
- identical protein binding Source: RGD
GO - Biological processi
- cellular response to dexamethasone stimulus Source: RGD
- cellular response to glucose stimulus Source: RGD
- cellular response to hypoxia Source: RGD
- cellular response to oxygen-glucose deprivation Source: RGD
- GTP metabolic process Source: UniProtKB
- intracellular distribution of mitochondria Source: RGD
- male gonad development Source: RGD
- mitochondrial fusion Source: UniProtKB
- mitochondrial membrane fusion Source: UniProtKB
- mitochondrion localization Source: UniProtKB
- mitochondrion morphogenesis Source: RGD
- negative regulation of mitochondrial fission Source: RGD
- positive regulation of dendritic spine morphogenesis Source: RGD
- positive regulation of mitochondrial fusion Source: RGD
- positive regulation of mitochondrial membrane potential Source: RGD
- response to calcium ion Source: RGD
- response to corticosterone Source: RGD
- response to electrical stimulus Source: RGD
- response to ethanol Source: RGD
- response to flavonoid Source: RGD
- response to fluoride Source: RGD
- response to hypobaric hypoxia Source: RGD
- response to manganese ion Source: RGD
- response to muscle activity Source: RGD
- response to nutrient levels Source: RGD
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-5205685, PINK1-PRKN Mediated Mitophagy R-RNO-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: Mitofusin-1 (EC:3.6.5.-By similarity)Alternative name(s): Mitochondrial transmembrane GTPase FZO1B Transmembrane GTPase MFN1 |
Gene namesi | Name:Mfn1 Synonyms:Fzo1b |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621460, Mfn1 |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane 1 Publication; Multi-pass membrane protein 1 Publication
Mitochondrion
- integral component of mitochondrial outer membrane Source: UniProtKB
- intrinsic component of mitochondrial outer membrane Source: GO_Central
- mitochondrial inner membrane Source: RGD
- mitochondrial outer membrane Source: RGD
- mitochondrion Source: RGD
- outer mitochondrial membrane protein complex Source: UniProtKB
Other locations
- integral component of membrane Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 584 | CytoplasmicSequence analysisAdd BLAST | 584 | |
Transmembranei | 585 – 605 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 606 – 608 | Mitochondrial intermembraneSequence analysis | 3 | |
Transmembranei | 609 – 629 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 630 – 741 | CytoplasmicSequence analysisAdd BLAST | 112 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 88 | K → T: Induces partial mitochondria fragmentation; when overexpressed. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127674 | 1 – 741 | Mitofusin-1Add BLAST | 741 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
jPOSTi | Q8R4Z9 |
PaxDbi | Q8R4Z9 |
PRIDEi | Q8R4Z9 |
PTM databases
iPTMneti | Q8R4Z9 |
PhosphoSitePlusi | Q8R4Z9 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Homodimer, also in the absence of bound GTP. Forms higher oligomers in the presence of a transition state GTP analog (By similarity). Forms homomultimers and heteromultimers with MFN2 (PubMed:14561718). Oligomerization is essential for mitochondrion fusion.
Component of a high molecular weight multiprotein complex (By similarity).
Interacts with VAT1 (PubMed:17105775).
Interacts with THG1L; THG1L probably functions as a guanyl-nucleotide exchange factor/GEF, activating MFN1.
By similarity2 PublicationsGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 251419, 1 interactor |
STRINGi | 10116.ENSRNOP00000060265 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 72 – 321 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 250 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 9 – 73 | Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST | 65 | |
Regioni | 82 – 89 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 108 – 109 | G2 motifPROSITE-ProRule annotation | 2 | |
Regioni | 178 – 181 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 237 – 240 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 266 | G5 motifPROSITE-ProRule annotation | 1 | |
Regioni | 338 – 364 | Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST | 27 | |
Regioni | 703 – 734 | Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST | 32 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 371 – 408 | Sequence analysisAdd BLAST | 38 | |
Coiled coili | 677 – 735 | Sequence analysisAdd BLAST | 59 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0448, Eukaryota |
InParanoidi | Q8R4Z9 |
OrthoDBi | 216494at2759 |
PhylomeDBi | Q8R4Z9 |
Family and domain databases
InterProi | View protein in InterPro IPR022812, Dynamin_SF IPR006884, Fzo/mitofusin_HR2 IPR030381, G_DYNAMIN_dom IPR027088, Mitofusin-1 IPR027094, Mitofusin_fam IPR027417, P-loop_NTPase |
PANTHERi | PTHR10465, PTHR10465, 1 hit PTHR10465:SF2, PTHR10465:SF2, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit PF04799, Fzo_mitofusin, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51718, G_DYNAMIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAETVSPLKH FVLAKKAITA IFGQLLEFVT EGSHFVEATY RNPELDRIAT
60 70 80 90 100
EDDLVEIQGY RNKLAVIGEV LSRRHMKVAF FGRTSSGKSS VINAMLWDKV
110 120 130 140 150
LPSGIGHTTN CFLSVEGTDG DKAYLMTEGS DEKKSVKTVN QLAHALHMDK
160 170 180 190 200
DLKAGCLVHV FWPKAKCALL RDDLVLVDSP GTDVTTELDI WIDKFCLDAD
210 220 230 240 250
VFVLVANSES TLMNTEKHFF HKVNERLSKP NIFILNNRWD ASASEPEYME
260 270 280 290 300
DVRRQHMERC LHFLVEELKV VSPLEARNRI FFVSAKEVLN SRMNKAQGMP
310 320 330 340 350
EGGGALAEGF QARLQEFQNF EQTFEECISQ SAVKTKFEQH TIRAKQILDT
360 370 380 390 400
VKNILDSVNV AAAEKRVYSM EEREDQIDRL DFIRNQMNLL TMDVKKKIKE
410 420 430 440 450
VTEEVANKVS CAMTDEICRL SVLVDEFCSE FHPTPSVLKV YKSELNKHIE
460 470 480 490 500
DGMGRNLADR CTSEVNASIL QSQQEIIENL KPLLPAGIQN KLHTLIPCKK
510 520 530 540 550
FDLSYDLNCH KLCSDFQEDI VFRFSLGWSS LVHRFLGSTN AQRVLLGLSE
560 570 580 590 600
PIFQVPRSLA STPTAPSNPA APDNAAQEEL MITLITGLAS LTSRTSMGII
610 620 630 640 650
VVGGVIWKTV GWKLISVTLS MYGALYLYER LTWTTRAKER AFKQQFVNYA
660 670 680 690 700
TEKLQMIVKF TSANCSHQVQ QEMATTFARL CQQVDVTQKH LEEEIARLSK
710 720 730 740
EIDQLEKIQN NSKLLRNKAV QLERELENFS KQFLHPSSGE S
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3ZR27 | D3ZR27_RAT | Mitofusin-1 | Mfn1 | 739 | Annotation score: | ||
A0A0G2K5J3 | A0A0G2K5J3_RAT | Mitofusin-1 | Mfn1 | 666 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB084166 mRNA Translation: BAB90983.1 |
RefSeqi | NP_620432.1, NM_138976.1 |
Genome annotation databases
GeneIDi | 192647 |
KEGGi | rno:192647 |
UCSCi | RGD:621460, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB084166 mRNA Translation: BAB90983.1 |
RefSeqi | NP_620432.1, NM_138976.1 |
3D structure databases
SMRi | Q8R4Z9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 251419, 1 interactor |
STRINGi | 10116.ENSRNOP00000060265 |
PTM databases
iPTMneti | Q8R4Z9 |
PhosphoSitePlusi | Q8R4Z9 |
Proteomic databases
jPOSTi | Q8R4Z9 |
PaxDbi | Q8R4Z9 |
PRIDEi | Q8R4Z9 |
Protocols and materials databases
ABCDi | Q8R4Z9, 1 sequenced antibody |
Genome annotation databases
GeneIDi | 192647 |
KEGGi | rno:192647 |
UCSCi | RGD:621460, rat |
Organism-specific databases
CTDi | 55669 |
RGDi | 621460, Mfn1 |
Phylogenomic databases
eggNOGi | KOG0448, Eukaryota |
InParanoidi | Q8R4Z9 |
OrthoDBi | 216494at2759 |
PhylomeDBi | Q8R4Z9 |
Enzyme and pathway databases
Reactomei | R-RNO-5205685, PINK1-PRKN Mediated Mitophagy R-RNO-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
PROi | PR:Q8R4Z9 |
Family and domain databases
InterProi | View protein in InterPro IPR022812, Dynamin_SF IPR006884, Fzo/mitofusin_HR2 IPR030381, G_DYNAMIN_dom IPR027088, Mitofusin-1 IPR027094, Mitofusin_fam IPR027417, P-loop_NTPase |
PANTHERi | PTHR10465, PTHR10465, 1 hit PTHR10465:SF2, PTHR10465:SF2, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit PF04799, Fzo_mitofusin, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51718, G_DYNAMIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MFN1_RAT | |
Accessioni | Q8R4Z9Primary (citable) accession number: Q8R4Z9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 102 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families