UniProtKB - Q8R4W6 (PCOC2_MOUSE)
Protein
Procollagen C-endopeptidase enhancer 2
Gene
Pcolce2
Organism
Mus musculus (Mouse)
Status
Functioni
Binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1.By similarity
GO - Molecular functioni
- collagen binding Source: MGI
- heparin binding Source: MGI
- peptidase activator activity Source: MGI
GO - Biological processi
- cellular response to leukemia inhibitory factor Source: MGI
Keywordsi
Molecular function | Heparin-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1650814, Collagen biosynthesis and modifying enzymes |
Names & Taxonomyi
Protein namesi | Recommended name: Procollagen C-endopeptidase enhancer 2Alternative name(s): Procollagen COOH-terminal proteinase enhancer 2 Short name: PCPE-2 Short name: Procollagen C-proteinase enhancer 2 |
Gene namesi | Name:Pcolce2 Synonyms:Pcpe2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1923727, Pcolce2 |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- collagen-containing extracellular matrix Source: BHF-UCL
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000022021 | 23 – 414 | Procollagen C-endopeptidase enhancer 2Add BLAST | 392 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 32 ↔ 58 | PROSITE-ProRule annotation | ||
Disulfide bondi | 85 ↔ 106 | PROSITE-ProRule annotation | ||
Disulfide bondi | 153 ↔ 180 | PROSITE-ProRule annotation | ||
Disulfide bondi | 207 ↔ 230 | PROSITE-ProRule annotation | ||
Disulfide bondi | 296 ↔ 363 | By similarity | ||
Disulfide bondi | 300 ↔ 366 | By similarity | ||
Disulfide bondi | 311 ↔ 414 | By similarity | ||
Glycosylationi | 354 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
O-glycosylated; contains sialic acid.By similarity
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | Q8R4W6 |
PaxDbi | Q8R4W6 |
PeptideAtlasi | Q8R4W6 |
PRIDEi | Q8R4W6 |
PTM databases
GlyGeni | Q8R4W6, 1 site |
PhosphoSitePlusi | Q8R4W6 |
Expressioni
Developmental stagei
Expressed at all stages of development, with expression level decreasing from 7 to 11 dpc and more abundant levels of expression at 15 and 17 dpc. First detectable at relatively low level at 10.5 dpc in the area of the third and fourth branchial arches. At 13.5 dpc easily discernible expression seems primarily confined to the cartilage primordia of future bones. At 15.5 dpc expressed at the highest levels in skeletal elements in the interior non-ossified regions of cartilaginous structures and excluded from regions of ossification.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000015354, Expressed in lung and 236 other tissues |
Genevisiblei | Q8R4W6, MM |
Interactioni
Subunit structurei
Interacts with heparin with high affinity, and type I or II collagen.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000015498 |
Miscellaneous databases
RNActi | Q8R4W6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 143 | CUB 1PROSITE-ProRule annotationAdd BLAST | 112 | |
Domaini | 153 – 267 | CUB 2PROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 296 – 414 | NTRPROSITE-ProRule annotationAdd BLAST | 119 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 273 – 277 | Poly-Thr | 5 |
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QRMG, Eukaryota |
GeneTreei | ENSGT00940000157649 |
HOGENOMi | CLU_034096_0_0_1 |
InParanoidi | Q8R4W6 |
OMAi | TEVCAQS |
OrthoDBi | 862783at2759 |
PhylomeDBi | Q8R4W6 |
TreeFami | TF316506 |
Family and domain databases
CDDi | cd00041, CUB, 2 hits cd03576, NTR_PCOLCE, 1 hit |
Gene3Di | 2.40.50.120, 1 hit 2.60.120.290, 2 hits |
InterProi | View protein in InterPro IPR000859, CUB_dom IPR001134, Netrin_domain IPR018933, Netrin_module_non-TIMP IPR035814, NTR_PCOLCE IPR035914, Sperma_CUB_dom_sf IPR008993, TIMP-like_OB-fold |
Pfami | View protein in Pfam PF00431, CUB, 2 hits PF01759, NTR, 1 hit |
SMARTi | View protein in SMART SM00643, C345C, 1 hit SM00042, CUB, 2 hits |
SUPFAMi | SSF49854, SSF49854, 2 hits SSF50242, SSF50242, 1 hit |
PROSITEi | View protein in PROSITE PS01180, CUB, 2 hits PS50189, NTR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8R4W6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGGASACIPL CLLLATARMA RPQTPERPVF TCGGILTGES GFIGSEGFPG
60 70 80 90 100
MYPPNSKCTW KITVPEGKVV VLNFRFIDLE NDNLCRYDFV DVYNGHANGQ
110 120 130 140 150
RIGRFCGTFR PGSLVASGNK MTVQMISDAN TAGSGFMATY SAAAPDGKGD
160 170 180 190 200
RYCGGRLEKP SGTFKTPNWP DRDYPVGVTC VWHIIAPKNQ LIELKFEKFD
210 220 230 240 250
VERDNYCRYD YVAVFNGGEV NDAKRIGKYC GDSPPVPIVS ERNELLIQFL
260 270 280 290 300
SDLSLTADGF IGHYKFRPKK FPTTTTTPVT TTLPVTTGLK PTVALCQQKC
310 320 330 340 350
RRMGTLESNY CSSNFVLAGT VITTVTRGGS LHATVSIISI YREGNLAIQQ
360 370 380 390 400
AGKNMSVKLT VVCRQCPLLR RGLNYIIMGQ VGEDGRGKIM PNSFVKMFKN
410
KNQKPMNALK NKQC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 167 | P → S in AAL83947 (PubMed:12393877).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF352788 mRNA Translation: AAL83947.1 AK010249 mRNA Translation: BAB26794.1 AK132395 mRNA Translation: BAE21144.1 BC051174 mRNA Translation: AAH51174.1 |
CCDSi | CCDS23410.1 |
RefSeqi | NP_083896.1, NM_029620.2 |
Genome annotation databases
Ensembli | ENSMUST00000015498; ENSMUSP00000015498; ENSMUSG00000015354 |
GeneIDi | 76477 |
KEGGi | mmu:76477 |
UCSCi | uc009rbg.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF352788 mRNA Translation: AAL83947.1 AK010249 mRNA Translation: BAB26794.1 AK132395 mRNA Translation: BAE21144.1 BC051174 mRNA Translation: AAH51174.1 |
CCDSi | CCDS23410.1 |
RefSeqi | NP_083896.1, NM_029620.2 |
3D structure databases
SMRi | Q8R4W6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000015498 |
PTM databases
GlyGeni | Q8R4W6, 1 site |
PhosphoSitePlusi | Q8R4W6 |
Proteomic databases
MaxQBi | Q8R4W6 |
PaxDbi | Q8R4W6 |
PeptideAtlasi | Q8R4W6 |
PRIDEi | Q8R4W6 |
Protocols and materials databases
Antibodypediai | 2145, 147 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000015498; ENSMUSP00000015498; ENSMUSG00000015354 |
GeneIDi | 76477 |
KEGGi | mmu:76477 |
UCSCi | uc009rbg.1, mouse |
Organism-specific databases
CTDi | 26577 |
MGIi | MGI:1923727, Pcolce2 |
Phylogenomic databases
eggNOGi | ENOG502QRMG, Eukaryota |
GeneTreei | ENSGT00940000157649 |
HOGENOMi | CLU_034096_0_0_1 |
InParanoidi | Q8R4W6 |
OMAi | TEVCAQS |
OrthoDBi | 862783at2759 |
PhylomeDBi | Q8R4W6 |
TreeFami | TF316506 |
Enzyme and pathway databases
Reactomei | R-MMU-1650814, Collagen biosynthesis and modifying enzymes |
Miscellaneous databases
BioGRID-ORCSi | 76477, 1 hit in 17 CRISPR screens |
ChiTaRSi | Pcolce2, mouse |
PROi | PR:Q8R4W6 |
RNActi | Q8R4W6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015354, Expressed in lung and 236 other tissues |
Genevisiblei | Q8R4W6, MM |
Family and domain databases
CDDi | cd00041, CUB, 2 hits cd03576, NTR_PCOLCE, 1 hit |
Gene3Di | 2.40.50.120, 1 hit 2.60.120.290, 2 hits |
InterProi | View protein in InterPro IPR000859, CUB_dom IPR001134, Netrin_domain IPR018933, Netrin_module_non-TIMP IPR035814, NTR_PCOLCE IPR035914, Sperma_CUB_dom_sf IPR008993, TIMP-like_OB-fold |
Pfami | View protein in Pfam PF00431, CUB, 2 hits PF01759, NTR, 1 hit |
SMARTi | View protein in SMART SM00643, C345C, 1 hit SM00042, CUB, 2 hits |
SUPFAMi | SSF49854, SSF49854, 2 hits SSF50242, SSF50242, 1 hit |
PROSITEi | View protein in PROSITE PS01180, CUB, 2 hits PS50189, NTR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PCOC2_MOUSE | |
Accessioni | Q8R4W6Primary (citable) accession number: Q8R4W6 Secondary accession number(s): Q3V1K6, Q9CX06 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | May 10, 2005 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot