UniProtKB - Q8R4T2 (SIAH2_RAT)
E3 ubiquitin-protein ligase SIAH2
Siah2
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 139 | Zinc 1By similarity | 1 | |
Metal bindingi | 146 | Zinc 1By similarity | 1 | |
Metal bindingi | 158 | Zinc 1By similarity | 1 | |
Metal bindingi | 162 | Zinc 1By similarity | 1 | |
Metal bindingi | 169 | Zinc 2By similarity | 1 | |
Metal bindingi | 176 | Zinc 2By similarity | 1 | |
Metal bindingi | 188 | Zinc 2By similarity | 1 | |
Metal bindingi | 193 | Zinc 2By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 81 – 116 | RING-typePROSITE-ProRule annotationAdd BLAST | 36 | |
Zinc fingeri | 134 – 194 | SIAH-typePROSITE-ProRule annotationAdd BLAST | 61 |
GO - Molecular functioni
- ubiquitin conjugating enzyme binding Source: RGD
- ubiquitin protein ligase activity Source: UniProtKB
- ubiquitin-protein transferase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell cycle Source: UniProtKB-KW
- cellular protein catabolic process Source: RGD
- multicellular organism development Source: InterPro
- negative regulation of apoptotic process Source: RGD
- negative regulation of canonical Wnt signaling pathway Source: RGD
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: RGD
- negative regulation of extrinsic apoptotic signaling pathway Source: RGD
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- regulation of circadian rhythm Source: UniProtKB
- regulation of protein ubiquitination Source: RGD
- rhythmic process Source: UniProtKB-KW
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Apoptosis, Biological rhythms, Cell cycle, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-373752, Netrin-1 signaling R-RNO-5689880, Ub-specific processing proteases R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase SIAH2 (EC:2.3.2.271 Publication)Alternative name(s): RING-type E3 ubiquitin transferase SIAH2Curated Seven in absentia homolog 2 Short name: Siah-2 |
Gene namesi | Name:Siah2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620778, Siah2 |
Subcellular locationi
Cytosol
- cytosol Source: RGD
Endosome
- early endosome Source: RGD
Nucleus
- nucleoplasm Source: Ensembl
Other locations
- cytoplasm Source: GO_Central
- neuron projection Source: RGD
- neuronal cell body Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056170 | 1 – 325 | E3 ubiquitin-protein ligase SIAH2Add BLAST | 325 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 6 | PhosphoserineBy similarity | 1 | |
Modified residuei | 16 | Phosphoserine; by DYRK2By similarity | 1 | |
Modified residuei | 24 | Phosphothreonine; by MAPK14By similarity | 1 | |
Modified residuei | 29 | Phosphoserine; by DYRK2 and MAPK14By similarity | 1 | |
Modified residuei | 69 | Phosphoserine; by DYRK2By similarity | 1 | |
Modified residuei | 120 | Phosphothreonine; by DYRK2By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8R4T2 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000013703, Expressed in testis and 22 other tissues |
Genevisiblei | Q8R4T2, RN |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with VAV1, without mediating its ubiquitin-mediated degradation (By similarity). Probable component of some large E3 complex possibly composed of UBE2D1, SIAH2, CACYBP/SIP, SKP1, APC and TBL1X (By similarity).
Interacts with UBE2I (By similarity).
Interacts with UBE2E2 (PubMed:11786535).
Interacts with PEG10, which may inhibit its activity (By similarity).
Interacts with PEG3 and EGLN2 (By similarity).
Interacts with DYRK2 (By similarity).
Interacts with SNCAIP (PubMed:15064394, PubMed:19224863).
Interacts with NR1D1 and NR1D2 (By similarity).
Interacts with DCC (By similarity).
By similarity3 PublicationsGO - Molecular functioni
- ubiquitin conjugating enzyme binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 250807, 2 interactors |
STRINGi | 10116.ENSRNOP00000018488 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 131 – 323 | SBDBy similarityAdd BLAST | 193 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 81 – 116 | RING-typePROSITE-ProRule annotationAdd BLAST | 36 | |
Zinc fingeri | 134 – 194 | SIAH-typePROSITE-ProRule annotationAdd BLAST | 61 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3002, Eukaryota |
GeneTreei | ENSGT00940000159812 |
HOGENOMi | CLU_028215_0_0_1 |
InParanoidi | Q8R4T2 |
OMAi | AGHLVCK |
OrthoDBi | 780610at2759 |
PhylomeDBi | Q8R4T2 |
Family and domain databases
Gene3Di | 2.60.210.10, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR018121, 7-in-absentia-prot_TRAF-dom IPR004162, SINA-like_animal IPR008974, TRAF-like IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR013010, Znf_SIAH |
PANTHERi | PTHR45877, PTHR45877, 1 hit |
Pfami | View protein in Pfam PF03145, Sina, 1 hit |
SUPFAMi | SSF49599, SSF49599, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit PS51081, ZF_SIAH, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSRPSSTGPS ANKPCSKQPP PPQTPHAPSP AAPPAAATIS AAGPGSSAVP
60 70 80 90 100
AAAAVISGPG AGGGAGPVSP QHHELTSLFE CPVCFDYVLP PILQCQAGHL
110 120 130 140 150
VCNQCRQKLS CCPTCRGALT PSIRNLAMEK VASAVLFPCK YATTGCSLTL
160 170 180 190 200
HHTEKPEHED ICEYRPYSCP CPGASCKWQG SLEAVMSHLM HAHKSITTLQ
210 220 230 240 250
GEDIVFLATD INLPGAVDWV MMQSCFGHHF MLVLEKQEKY EGHQQFFAIV
260 270 280 290 300
LLIGTRKQAE NFAYRLELNG NRRRLTWEAT PRSIHDGVAA AIMNSDCLVF
310 320
DTAIAHLFAD NGNLGINVTI STCCQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB067815 mRNA Translation: BAB70754.1 AF389477 mRNA Translation: AAL91363.1 |
RefSeqi | NP_604452.1, NM_134457.1 |
Genome annotation databases
Ensembli | ENSRNOT00000018488; ENSRNOP00000018488; ENSRNOG00000013703 |
GeneIDi | 140593 |
KEGGi | rno:140593 |
UCSCi | RGD:620778, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB067815 mRNA Translation: BAB70754.1 AF389477 mRNA Translation: AAL91363.1 |
RefSeqi | NP_604452.1, NM_134457.1 |
3D structure databases
SMRi | Q8R4T2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 250807, 2 interactors |
STRINGi | 10116.ENSRNOP00000018488 |
Proteomic databases
PaxDbi | Q8R4T2 |
Genome annotation databases
Ensembli | ENSRNOT00000018488; ENSRNOP00000018488; ENSRNOG00000013703 |
GeneIDi | 140593 |
KEGGi | rno:140593 |
UCSCi | RGD:620778, rat |
Organism-specific databases
CTDi | 6478 |
RGDi | 620778, Siah2 |
Phylogenomic databases
eggNOGi | KOG3002, Eukaryota |
GeneTreei | ENSGT00940000159812 |
HOGENOMi | CLU_028215_0_0_1 |
InParanoidi | Q8R4T2 |
OMAi | AGHLVCK |
OrthoDBi | 780610at2759 |
PhylomeDBi | Q8R4T2 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-RNO-373752, Netrin-1 signaling R-RNO-5689880, Ub-specific processing proteases R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:Q8R4T2 |
Gene expression databases
Bgeei | ENSRNOG00000013703, Expressed in testis and 22 other tissues |
Genevisiblei | Q8R4T2, RN |
Family and domain databases
Gene3Di | 2.60.210.10, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR018121, 7-in-absentia-prot_TRAF-dom IPR004162, SINA-like_animal IPR008974, TRAF-like IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR013010, Znf_SIAH |
PANTHERi | PTHR45877, PTHR45877, 1 hit |
Pfami | View protein in Pfam PF03145, Sina, 1 hit |
SUPFAMi | SSF49599, SSF49599, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit PS51081, ZF_SIAH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SIAH2_RAT | |
Accessioni | Q8R4T2Primary (citable) accession number: Q8R4T2 Secondary accession number(s): Q920M8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | April 26, 2004 | |
Last modified: | April 7, 2021 | |
This is version 131 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families