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Protein

Src-like-adapter 2

Gene

Sla2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.2 Publications

Miscellaneous

PubMed:12024036 confirmed the alternative initiation by mutating the Met in position 1 to Val, and showed that isoform 1 is abolished in favor of isoform 2.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Src-like-adapter 2
Alternative name(s):
Src-like adapter protein 2
Short name:
SLAP-2
Gene namesi
Name:Sla2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1925049 Sla2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Abolishes localization to membranes. 2 Publications1
Mutagenesisi27M → V: Abolishes isoform 2. 1 Publication1
Mutagenesisi82P → L: Does not affect its inhibitory function. 1 Publication1
Mutagenesisi120R → E: Abolishes interaction with ZAP70, and its inhibitory function. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000223532 – 259Src-like-adapter 2Add BLAST258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine2 Publications1

Post-translational modificationi

Phosphorylated by CSF1R.2 Publications

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ8R4L0
PRIDEiQ8R4L0

PTM databases

iPTMnetiQ8R4L0
PhosphoSitePlusiQ8R4L0

Expressioni

Tissue specificityi

Mainly expressed in immune system. Highly expressed in spleen and thymus and expressed at intermediate levels in lung. Not expressed in liver, heart and brain. Isoform 1 is predominant in lung and spleen, while isoform 2 is predominant in thymus.

Gene expression databases

BgeeiENSMUSG00000027636 Expressed in 39 organ(s), highest expression level in thymus
CleanExiMM_SLA2
GenevisibleiQ8R4L0 MM

Interactioni

Subunit structurei

Interacts (via its C-terminal domain) with CBL (phosphorylated). Interacts (via SH2 domain) with ZAP70 (phosphorylated) and CD3Z (phosphorylated). Interacts (via SH2 domain) with CSF1R (phosphorylated).3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi218930, 2 interactors
STRINGi10090.ENSMUSP00000029164

Structurei

3D structure databases

ProteinModelPortaliQ8R4L0
SMRiQ8R4L0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 91SH3PROSITE-ProRule annotationAdd BLAST61
Domaini93 – 190SH2PROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 259SLA C-terminalAdd BLAST70

Domaini

The loss of the C-terminal domain partially abolishes the inhibitory function.

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiENOG410IKI6 Eukaryota
ENOG4111GMN LUCA
GeneTreeiENSGT00390000001681
HOGENOMiHOG000234240
HOVERGENiHBG054908
InParanoidiQ8R4L0
OMAiKISHGWL
OrthoDBiEOG091G0GB0
PhylomeDBiQ8R4L0
TreeFamiTF354288

Family and domain databases

CDDicd10344 SH2_SLAP, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035054 SLAP2
IPR035052 SLAP_SH2
PANTHERiPTHR24418:SF376 PTHR24418:SF376, 1 hit
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
PS50002 SH3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform 1 (identifier: Q8R4L0-1) [UniParc]FASTAAdd to basket
Also known as: p28

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGSLSSRGKT SSPSPSSSGP DQEPVSMQPE RHKVTAVALG SFPAGEQARL
60 70 80 90 100
SLRLGEPLTI ISEDGDWWTV QSEVSGREYH MPSVYVAKVA HGWLYEGLSR
110 120 130 140 150
EKAEELLLLP GNPGGAFLIR ESQTRRGCYS LSVRLSRPAS WDRIRHYRIQ
160 170 180 190 200
RLDNGWLYIS PRLTFPSLHA LVEHYSELAD GICCPLREPC VLQKLGPLPG
210 220 230 240 250
KDTPPPVTVP TSSLNWKKLD RSLLFLEAPA SGEASLLSEG LRESLSSYIS

LAEDPLDDA
Length:259
Mass (Da):28,476
Last modified:January 23, 2007 - v3
Checksum:i8270F17CD3FC50A3
GO
Isoform 2 (identifier: Q8R4L0-2) [UniParc]FASTAAdd to basket
Also known as: p25

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:233
Mass (Da):25,945
Checksum:i52C71875503F4AEB
GO

Sequence cautioni

The sequence BAB32223 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128C → Y in BAC27168 (PubMed:16141072).Curated1
Sequence conflicti160S → T in BAC27168 (PubMed:16141072).Curated1
Sequence conflicti161P → H in AAL86403 (PubMed:11891219).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0187951 – 26Missing in isoform 2. 2 PublicationsAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287467 mRNA Translation: AAL38196.1
AF434990 mRNA Translation: AAL86403.1
AK020837 mRNA Translation: BAB32223.1 Different initiation.
AK030877 mRNA Translation: BAC27168.1
AK088672 mRNA Translation: BAC40495.1
AK138709 mRNA Translation: BAE23754.1
CCDSiCCDS38302.1 [Q8R4L0-1]
RefSeqiNP_084259.1, NM_029983.5 [Q8R4L0-1]
XP_006500468.1, XM_006500405.2
UniGeneiMm.29264
Mm.387161

Genome annotation databases

EnsembliENSMUST00000029164; ENSMUSP00000029164; ENSMUSG00000027636 [Q8R4L0-1]
ENSMUST00000109561; ENSMUSP00000105189; ENSMUSG00000027636 [Q8R4L0-1]
GeneIDi77799
KEGGimmu:77799
UCSCiuc008nod.1 mouse [Q8R4L0-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287467 mRNA Translation: AAL38196.1
AF434990 mRNA Translation: AAL86403.1
AK020837 mRNA Translation: BAB32223.1 Different initiation.
AK030877 mRNA Translation: BAC27168.1
AK088672 mRNA Translation: BAC40495.1
AK138709 mRNA Translation: BAE23754.1
CCDSiCCDS38302.1 [Q8R4L0-1]
RefSeqiNP_084259.1, NM_029983.5 [Q8R4L0-1]
XP_006500468.1, XM_006500405.2
UniGeneiMm.29264
Mm.387161

3D structure databases

ProteinModelPortaliQ8R4L0
SMRiQ8R4L0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218930, 2 interactors
STRINGi10090.ENSMUSP00000029164

PTM databases

iPTMnetiQ8R4L0
PhosphoSitePlusiQ8R4L0

Proteomic databases

PaxDbiQ8R4L0
PRIDEiQ8R4L0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029164; ENSMUSP00000029164; ENSMUSG00000027636 [Q8R4L0-1]
ENSMUST00000109561; ENSMUSP00000105189; ENSMUSG00000027636 [Q8R4L0-1]
GeneIDi77799
KEGGimmu:77799
UCSCiuc008nod.1 mouse [Q8R4L0-1]

Organism-specific databases

CTDi84174
MGIiMGI:1925049 Sla2

Phylogenomic databases

eggNOGiENOG410IKI6 Eukaryota
ENOG4111GMN LUCA
GeneTreeiENSGT00390000001681
HOGENOMiHOG000234240
HOVERGENiHBG054908
InParanoidiQ8R4L0
OMAiKISHGWL
OrthoDBiEOG091G0GB0
PhylomeDBiQ8R4L0
TreeFamiTF354288

Miscellaneous databases

PROiPR:Q8R4L0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027636 Expressed in 39 organ(s), highest expression level in thymus
CleanExiMM_SLA2
GenevisibleiQ8R4L0 MM

Family and domain databases

CDDicd10344 SH2_SLAP, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035054 SLAP2
IPR035052 SLAP_SH2
PANTHERiPTHR24418:SF376 PTHR24418:SF376, 1 hit
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSLAP2_MOUSE
AccessioniPrimary (citable) accession number: Q8R4L0
Secondary accession number(s): Q3UU74
, Q8C0K2, Q8VI42, Q9D1Z9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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