UniProtKB - Q8R4I7 (NETO1_MOUSE)
Protein
Neuropilin and tolloid-like protein 1
Gene
Neto1
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.2 Publications
GO - Molecular functioni
- ionotropic glutamate receptor binding Source: MGI
GO - Biological processi
- memory Source: UniProtKB
- positive regulation of excitatory postsynaptic potential Source: MGI
- receptor localization to synapse Source: MGI
- regulation of kainate selective glutamate receptor activity Source: MGI
- regulation of long-term neuronal synaptic plasticity Source: UniProtKB
- visual learning Source: UniProtKB
Keywordsi
Molecular function | Receptor |
Names & Taxonomyi
Protein namesi | Recommended name: Neuropilin and tolloid-like protein 1Alternative name(s): Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 |
Gene namesi | Name:Neto1 Synonyms:Btcl1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2180216 Neto1 |
Subcellular locationi
Plasma membrane
- postsynaptic density membrane 1 Publication
Other locations
- Membrane Curated; Single-pass type I membrane protein Curated
Note: Component of the postsynaptic density (PSD) of excitatory synapses.
Plasma Membrane
- integral component of postsynaptic density membrane Source: SynGO
- postsynaptic density membrane Source: SynGO
Other locations
- cell junction Source: UniProtKB-KW
- excitatory synapse Source: UniProtKB
- glutamatergic synapse Source: SynGO
- postsynaptic density Source: UniProtKB
- synapse Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 23 – 344 | ExtracellularSequence analysisAdd BLAST | 322 | |
Transmembranei | 345 – 365 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 366 – 533 | CytoplasmicSequence analysisAdd BLAST | 168 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Disruption phenotypei
Depressed long-term potentiation (LTP) at Schaffer collateral-CA1 synapses, NMDAR-dependent spatial learning and memory. Rescued by the ampakine CX546 at physiological doses.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000021800 | 23 – 533 | Neuropilin and tolloid-like protein 1Add BLAST | 511 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 41 ↔ 68 | By similarity | ||
Disulfide bondi | 96 ↔ 118 | By similarity | ||
Disulfide bondi | 172 ↔ 202 | By similarity | ||
Disulfide bondi | 229 ↔ 251 | By similarity | ||
Disulfide bondi | 292 ↔ 304 | By similarity | ||
Disulfide bondi | 299 ↔ 317 | By similarity | ||
Glycosylationi | 306 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 311 ↔ 326 | By similarity | ||
Glycosylationi | 340 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 417 | PhosphotyrosineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q8R4I7 |
PeptideAtlasi | Q8R4I7 |
PRIDEi | Q8R4I7 |
PTM databases
iPTMneti | Q8R4I7 |
PhosphoSitePlusi | Q8R4I7 |
Expressioni
Tissue specificityi
Expressed only in brain. Present throughout the central nervous system. Highly expressed in the hippocampal CA3 region, olfactory bulb and tubercle, caudate putamen, and neocortex in the adult brain.3 Publications
Developmental stagei
Observed restrictively in brain throughout embryonic and postnatal stages. Expression pattern in brain slightly changes from 13 dpc to postnatal day 21 (P21). Expressed in both cerebrum and cerebellum throughout P0 to P35. In the cerebrum expression reached a plateau at P14 while expression in the cerebellum remains constant throughout all postnatal stages.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000050321 Expressed in 108 organ(s), highest expression level in striatum |
Genevisiblei | Q8R4I7 MM |
Interactioni
Subunit structurei
Interacts with PLZ domains of DLG2, DLG3 and DLG4 via its C-terminal TRV domain.
Interacts with GRIN2A and GRIN2B via its CUB domains.
1 PublicationBinary interactionsi
GO - Molecular functioni
- ionotropic glutamate receptor binding Source: MGI
Protein-protein interaction databases
BioGridi | 232925, 1 interactor |
DIPi | DIP-48395N |
IntActi | Q8R4I7, 10 interactors |
STRINGi | 10090.ENSMUSP00000057340 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 155 | CUB 1PROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 172 – 287 | CUB 2PROSITE-ProRule annotationAdd BLAST | 116 | |
Domaini | 291 – 327 | LDL-receptor class APROSITE-ProRule annotationAdd BLAST | 37 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 531 – 533 | PDZ-binding | 3 |
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG410IJXA Eukaryota ENOG410ZS19 LUCA |
GeneTreei | ENSGT00940000156700 |
HOGENOMi | HOG000060303 |
InParanoidi | Q8R4I7 |
OMAi | CEANTFF |
OrthoDBi | 477476at2759 |
PhylomeDBi | Q8R4I7 |
Family and domain databases
CDDi | cd00041 CUB, 2 hits cd00112 LDLa, 1 hit |
Gene3Di | 2.60.120.290, 2 hits |
InterProi | View protein in InterPro IPR000859 CUB_dom IPR036055 LDL_receptor-like_sf IPR023415 LDLR_class-A_CS IPR002172 LDrepeatLR_classA_rpt IPR028867 NETO1 IPR035914 Sperma_CUB_dom_sf |
PANTHERi | PTHR45645:SF3 PTHR45645:SF3, 1 hit |
Pfami | View protein in Pfam PF00431 CUB, 2 hits PF00057 Ldl_recept_a, 1 hit |
SMARTi | View protein in SMART SM00042 CUB, 2 hits SM00192 LDLa, 1 hit |
SUPFAMi | SSF49854 SSF49854, 2 hits SSF57424 SSF57424, 1 hit |
PROSITEi | View protein in PROSITE PS01180 CUB, 2 hits PS01209 LDLRA_1, 1 hit PS50068 LDLRA_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8R4I7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIYGRSLFHI IASLIILHSS GATKKGTEKQ ITPETQKSVQ CGTWTKHAEG
60 70 80 90 100
GVFTSPNYPS KYPPDRECVY IIEAAPRQCI ELYFDEKYSI EPSWECKFDH
110 120 130 140 150
IEVRDGPFGF SPIIGRFCGQ QNPPVIKSSG RFLWIKFFAD GELESMGFSA
160 170 180 190 200
RYNFTPDPDF KDLGVLKPLP ACEFEMGGPE GIVESIQILK EGKASASEAV
210 220 230 240 250
DCKWYIRAPP RSKIYLRFLD YEMQNSNECK RNFVAVYDGS SSVEDLKAKF
260 270 280 290 300
CSTVANDVML RTGLGVIRMW ADEGSRNSRF QMLFTSFQEP PCEGNTFFCH
310 320 330 340 350
SNMCINNTLV CNGLQNCVYP WDENHCKEKR KTSLLDQLTN TSGTVIGVTS
360 370 380 390 400
CIVIILIIVS VIVQIKQPRK KYVQRKSDFD QTVFQEVFEP PHYELCTLRG
410 420 430 440 450
TGATADFADV AEDFENYHKL RRSSSKCIHD HHCGSQLSSA KGSRSNLSTR
460 470 480 490 500
DASILAEIPT QPVKPLIPPV NRRNILVMKH NYSQDAADAC DIDEIEEVPT
510 520 530
TSHRLSRHEK SVQRFCLIGS LSKHESEYNT TRV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A494BAI8 | A0A494BAI8_MOUSE | Neuropilin and tolloid-like protein... | Neto1 | 68 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 80 | I → F in BAC38196 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 514 | R → Q in AAM18028 (PubMed:11943477).Curated | 1 | |
Sequence conflicti | 514 | R → Q in BAC38196 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF448840 mRNA Translation: AAM18028.1 AY138990 mRNA Translation: AAN38318.1 AK032510 mRNA Translation: BAC27902.1 AK081325 mRNA Translation: BAC38196.1 BC051145 mRNA Translation: AAH51145.1 |
CCDSi | CCDS29387.1 |
RefSeqi | NP_659195.3, NM_144946.4 XP_006526543.1, XM_006526480.3 XP_006526544.1, XM_006526481.3 XP_006526545.1, XM_006526482.3 XP_017173396.1, XM_017317907.1 XP_017173397.1, XM_017317908.1 |
Genome annotation databases
Ensembli | ENSMUST00000058829; ENSMUSP00000057340; ENSMUSG00000050321 |
GeneIDi | 246317 |
KEGGi | mmu:246317 |
UCSCi | uc008fuz.2 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF448840 mRNA Translation: AAM18028.1 AY138990 mRNA Translation: AAN38318.1 AK032510 mRNA Translation: BAC27902.1 AK081325 mRNA Translation: BAC38196.1 BC051145 mRNA Translation: AAH51145.1 |
CCDSi | CCDS29387.1 |
RefSeqi | NP_659195.3, NM_144946.4 XP_006526543.1, XM_006526480.3 XP_006526544.1, XM_006526481.3 XP_006526545.1, XM_006526482.3 XP_017173396.1, XM_017317907.1 XP_017173397.1, XM_017317908.1 |
3D structure databases
SMRi | Q8R4I7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 232925, 1 interactor |
DIPi | DIP-48395N |
IntActi | Q8R4I7, 10 interactors |
STRINGi | 10090.ENSMUSP00000057340 |
PTM databases
iPTMneti | Q8R4I7 |
PhosphoSitePlusi | Q8R4I7 |
Proteomic databases
PaxDbi | Q8R4I7 |
PeptideAtlasi | Q8R4I7 |
PRIDEi | Q8R4I7 |
Genome annotation databases
Ensembli | ENSMUST00000058829; ENSMUSP00000057340; ENSMUSG00000050321 |
GeneIDi | 246317 |
KEGGi | mmu:246317 |
UCSCi | uc008fuz.2 mouse |
Organism-specific databases
CTDi | 81832 |
MGIi | MGI:2180216 Neto1 |
Phylogenomic databases
eggNOGi | ENOG410IJXA Eukaryota ENOG410ZS19 LUCA |
GeneTreei | ENSGT00940000156700 |
HOGENOMi | HOG000060303 |
InParanoidi | Q8R4I7 |
OMAi | CEANTFF |
OrthoDBi | 477476at2759 |
PhylomeDBi | Q8R4I7 |
Miscellaneous databases
ChiTaRSi | Neto1 mouse |
PROi | PR:Q8R4I7 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000050321 Expressed in 108 organ(s), highest expression level in striatum |
Genevisiblei | Q8R4I7 MM |
Family and domain databases
CDDi | cd00041 CUB, 2 hits cd00112 LDLa, 1 hit |
Gene3Di | 2.60.120.290, 2 hits |
InterProi | View protein in InterPro IPR000859 CUB_dom IPR036055 LDL_receptor-like_sf IPR023415 LDLR_class-A_CS IPR002172 LDrepeatLR_classA_rpt IPR028867 NETO1 IPR035914 Sperma_CUB_dom_sf |
PANTHERi | PTHR45645:SF3 PTHR45645:SF3, 1 hit |
Pfami | View protein in Pfam PF00431 CUB, 2 hits PF00057 Ldl_recept_a, 1 hit |
SMARTi | View protein in SMART SM00042 CUB, 2 hits SM00192 LDLa, 1 hit |
SUPFAMi | SSF49854 SSF49854, 2 hits SSF57424 SSF57424, 1 hit |
PROSITEi | View protein in PROSITE PS01180 CUB, 2 hits PS01209 LDLRA_1, 1 hit PS50068 LDLRA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NETO1_MOUSE | |
Accessioni | Q8R4I7Primary (citable) accession number: Q8R4I7 Secondary accession number(s): Q80X39, Q8C4S3, Q8CCM2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 15, 2005 |
Last sequence update: | February 15, 2005 | |
Last modified: | November 13, 2019 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot