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Protein

Neuropilin and tolloid-like protein 1

Gene

Neto1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.2 Publications

GO - Molecular functioni

  • ionotropic glutamate receptor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionReceptor

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropilin and tolloid-like protein 1
Alternative name(s):
Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1
Gene namesi
Name:Neto1
Synonyms:Btcl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2180216 Neto1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 344ExtracellularSequence analysisAdd BLAST322
Transmembranei345 – 365HelicalSequence analysisAdd BLAST21
Topological domaini366 – 533CytoplasmicSequence analysisAdd BLAST168

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Depressed long-term potentiation (LTP) at Schaffer collateral-CA1 synapses, NMDAR-dependent spatial learning and memory. Rescued by the ampakine CX546 at physiological doses.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000002180023 – 533Neuropilin and tolloid-like protein 1Add BLAST511

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 68By similarity
Disulfide bondi96 ↔ 118By similarity
Disulfide bondi172 ↔ 202By similarity
Disulfide bondi229 ↔ 251By similarity
Disulfide bondi292 ↔ 304By similarity
Disulfide bondi299 ↔ 317By similarity
Glycosylationi306N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi311 ↔ 326By similarity
Glycosylationi340N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei417PhosphotyrosineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8R4I7
PeptideAtlasiQ8R4I7
PRIDEiQ8R4I7

PTM databases

iPTMnetiQ8R4I7
PhosphoSitePlusiQ8R4I7

Expressioni

Tissue specificityi

Expressed only in brain. Present throughout the central nervous system. Highly expressed in the hippocampal CA3 region, olfactory bulb and tubercle, caudate putamen, and neocortex in the adult brain.3 Publications

Developmental stagei

Observed restrictively in brain throughout embryonic and postnatal stages. Expression pattern in brain slightly changes from E13 to postnatal day 21 (P21). Expressed in both cerebrum and cerebellum throughout P0 to P35. In the cerebrum expression reached a plateau at P14 while expression in the cerebellum remains constant throughout all postnatal stages.2 Publications

Gene expression databases

BgeeiENSMUSG00000050321 Expressed in 108 organ(s), highest expression level in striatum
CleanExiMM_NETO1
GenevisibleiQ8R4I7 MM

Interactioni

Subunit structurei

Interacts with PLZ domains of DLG2, DLG3 and DLG4 via its C-terminal TRV domain. Interacts with GRIN2A and GRIN2B via its CUB domains.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi232925, 1 interactor
DIPiDIP-48395N
IntActiQ8R4I7, 10 interactors
STRINGi10090.ENSMUSP00000057340

Structurei

3D structure databases

ProteinModelPortaliQ8R4I7
SMRiQ8R4I7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 155CUB 1PROSITE-ProRule annotationAdd BLAST115
Domaini172 – 287CUB 2PROSITE-ProRule annotationAdd BLAST116
Domaini291 – 327LDL-receptor class APROSITE-ProRule annotationAdd BLAST37

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi531 – 533PDZ-binding3

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJXA Eukaryota
ENOG410ZS19 LUCA
GeneTreeiENSGT00760000119018
HOGENOMiHOG000060303
HOVERGENiHBG052600
InParanoidiQ8R4I7
OMAiTEAVDCK
OrthoDBiEOG091G060O
PhylomeDBiQ8R4I7

Family and domain databases

CDDicd00041 CUB, 2 hits
cd00112 LDLa, 1 hit
Gene3Di2.60.120.290, 2 hits
InterProiView protein in InterPro
IPR000859 CUB_dom
IPR036055 LDL_receptor-like_sf
IPR023415 LDLR_class-A_CS
IPR002172 LDrepeatLR_classA_rpt
IPR028867 NETO1
IPR035914 Sperma_CUB_dom_sf
PANTHERiPTHR44143:SF1 PTHR44143:SF1, 1 hit
PfamiView protein in Pfam
PF00431 CUB, 2 hits
PF00057 Ldl_recept_a, 1 hit
SMARTiView protein in SMART
SM00042 CUB, 2 hits
SM00192 LDLa, 1 hit
SUPFAMiSSF49854 SSF49854, 2 hits
SSF57424 SSF57424, 1 hit
PROSITEiView protein in PROSITE
PS01180 CUB, 2 hits
PS01209 LDLRA_1, 1 hit
PS50068 LDLRA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R4I7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIYGRSLFHI IASLIILHSS GATKKGTEKQ ITPETQKSVQ CGTWTKHAEG
60 70 80 90 100
GVFTSPNYPS KYPPDRECVY IIEAAPRQCI ELYFDEKYSI EPSWECKFDH
110 120 130 140 150
IEVRDGPFGF SPIIGRFCGQ QNPPVIKSSG RFLWIKFFAD GELESMGFSA
160 170 180 190 200
RYNFTPDPDF KDLGVLKPLP ACEFEMGGPE GIVESIQILK EGKASASEAV
210 220 230 240 250
DCKWYIRAPP RSKIYLRFLD YEMQNSNECK RNFVAVYDGS SSVEDLKAKF
260 270 280 290 300
CSTVANDVML RTGLGVIRMW ADEGSRNSRF QMLFTSFQEP PCEGNTFFCH
310 320 330 340 350
SNMCINNTLV CNGLQNCVYP WDENHCKEKR KTSLLDQLTN TSGTVIGVTS
360 370 380 390 400
CIVIILIIVS VIVQIKQPRK KYVQRKSDFD QTVFQEVFEP PHYELCTLRG
410 420 430 440 450
TGATADFADV AEDFENYHKL RRSSSKCIHD HHCGSQLSSA KGSRSNLSTR
460 470 480 490 500
DASILAEIPT QPVKPLIPPV NRRNILVMKH NYSQDAADAC DIDEIEEVPT
510 520 530
TSHRLSRHEK SVQRFCLIGS LSKHESEYNT TRV
Length:533
Mass (Da):60,242
Last modified:February 15, 2005 - v2
Checksum:i6375B395421D558F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80I → F in BAC38196 (PubMed:16141072).Curated1
Sequence conflicti514R → Q in AAM18028 (PubMed:11943477).Curated1
Sequence conflicti514R → Q in BAC38196 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448840 mRNA Translation: AAM18028.1
AY138990 mRNA Translation: AAN38318.1
AK032510 mRNA Translation: BAC27902.1
AK081325 mRNA Translation: BAC38196.1
BC051145 mRNA Translation: AAH51145.1
CCDSiCCDS29387.1
RefSeqiNP_659195.3, NM_144946.4
XP_006526543.1, XM_006526480.3
XP_006526544.1, XM_006526481.3
XP_006526545.1, XM_006526482.3
XP_017173396.1, XM_017317907.1
XP_017173397.1, XM_017317908.1
UniGeneiMm.329578

Genome annotation databases

EnsembliENSMUST00000058829; ENSMUSP00000057340; ENSMUSG00000050321
GeneIDi246317
KEGGimmu:246317
UCSCiuc008fuz.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448840 mRNA Translation: AAM18028.1
AY138990 mRNA Translation: AAN38318.1
AK032510 mRNA Translation: BAC27902.1
AK081325 mRNA Translation: BAC38196.1
BC051145 mRNA Translation: AAH51145.1
CCDSiCCDS29387.1
RefSeqiNP_659195.3, NM_144946.4
XP_006526543.1, XM_006526480.3
XP_006526544.1, XM_006526481.3
XP_006526545.1, XM_006526482.3
XP_017173396.1, XM_017317907.1
XP_017173397.1, XM_017317908.1
UniGeneiMm.329578

3D structure databases

ProteinModelPortaliQ8R4I7
SMRiQ8R4I7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232925, 1 interactor
DIPiDIP-48395N
IntActiQ8R4I7, 10 interactors
STRINGi10090.ENSMUSP00000057340

PTM databases

iPTMnetiQ8R4I7
PhosphoSitePlusiQ8R4I7

Proteomic databases

PaxDbiQ8R4I7
PeptideAtlasiQ8R4I7
PRIDEiQ8R4I7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058829; ENSMUSP00000057340; ENSMUSG00000050321
GeneIDi246317
KEGGimmu:246317
UCSCiuc008fuz.2 mouse

Organism-specific databases

CTDi81832
MGIiMGI:2180216 Neto1

Phylogenomic databases

eggNOGiENOG410IJXA Eukaryota
ENOG410ZS19 LUCA
GeneTreeiENSGT00760000119018
HOGENOMiHOG000060303
HOVERGENiHBG052600
InParanoidiQ8R4I7
OMAiTEAVDCK
OrthoDBiEOG091G060O
PhylomeDBiQ8R4I7

Miscellaneous databases

ChiTaRSiNeto1 mouse
PROiPR:Q8R4I7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050321 Expressed in 108 organ(s), highest expression level in striatum
CleanExiMM_NETO1
GenevisibleiQ8R4I7 MM

Family and domain databases

CDDicd00041 CUB, 2 hits
cd00112 LDLa, 1 hit
Gene3Di2.60.120.290, 2 hits
InterProiView protein in InterPro
IPR000859 CUB_dom
IPR036055 LDL_receptor-like_sf
IPR023415 LDLR_class-A_CS
IPR002172 LDrepeatLR_classA_rpt
IPR028867 NETO1
IPR035914 Sperma_CUB_dom_sf
PANTHERiPTHR44143:SF1 PTHR44143:SF1, 1 hit
PfamiView protein in Pfam
PF00431 CUB, 2 hits
PF00057 Ldl_recept_a, 1 hit
SMARTiView protein in SMART
SM00042 CUB, 2 hits
SM00192 LDLa, 1 hit
SUPFAMiSSF49854 SSF49854, 2 hits
SSF57424 SSF57424, 1 hit
PROSITEiView protein in PROSITE
PS01180 CUB, 2 hits
PS01209 LDLRA_1, 1 hit
PS50068 LDLRA_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNETO1_MOUSE
AccessioniPrimary (citable) accession number: Q8R4I7
Secondary accession number(s): Q80X39, Q8C4S3, Q8CCM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: November 7, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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