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Protein

Neuronal acetylcholine receptor subunit alpha-3

Gene

Chrna3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-3
Gene namesi
Name:Chrna3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:87887 Chrna3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 234ExtracellularSequence analysisAdd BLAST209
Transmembranei235 – 259HelicalSequence analysisAdd BLAST25
Transmembranei267 – 285HelicalSequence analysisAdd BLAST19
Transmembranei301 – 322HelicalSequence analysisAdd BLAST22
Topological domaini323 – 471CytoplasmicSequence analysisAdd BLAST149
Transmembranei472 – 491HelicalSequence analysisAdd BLAST20

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3885609

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000034726 – 499Neuronal acetylcholine receptor subunit alpha-3Add BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi49N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi153 ↔ 167By similarity
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi217 ↔ 218Associated with receptor activationBy similarity
Modified residuei407PhosphoserineBy similarity1
Modified residuei410PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8R4G9
PaxDbiQ8R4G9
PRIDEiQ8R4G9

PTM databases

PhosphoSitePlusiQ8R4G9

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-3 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly. Interacts with LYPD6. The heteropentamer alpha-3-beta-2 interacts with alpha-conotoxins ImI, ImII, PnIA, GID and MII. The heteropentamer alpha-3-beta-4 interacts with the alpha-conotoxin ImI.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi225944, 2 interactors
ComplexPortaliCPX-188 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta2
CPX-189 Neuronal nicotinic acetylcholine receptor complex, alpha3-beta2
CPX-202 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta2-beta3
CPX-204 Neuronal nicotinic acetylcholine receptor complex, alpha3-beta4
CPX-209 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta4
CPX-212 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta4
STRINGi10090.ENSMUSP00000034851

Structurei

3D structure databases

ProteinModelPortaliQ8R4G9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
HOVERGENiHBG003756
InParanoidiQ8R4G9
KOiK04805
PhylomeDBiQ8R4G9

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8R4G9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGVVLPPPPL SMLMLVLMLL PVASASEAEH RLFQYLFEDY NEIIRPVANV
60 70 80 90 100
SHPVIIQFEV SMSQLVKVDE VNQIMETNLW LKQIWNDYKL KWKPSDYQGV
110 120 130 140 150
EFMRVPAEKI WKPDIVLYNN ADGDFQVDDK TKALLKYTGE VTWIPPAIFK
160 170 180 190 200
SSCKIDVTYF PFDYQNCTMK FGSWSYDKAK IDLVLIGSSM NLKDYWESGE
210 220 230 240 250
WAIIKAPGYK HEIKYNCCEE IYQDITYSLY IRRLPLFYTI NLIIPCLLIS
260 270 280 290 300
FLTVLVFYLP SDCGEKVTLC ISVLLSLTVF LLVITETIPS TSLVIPLIGE
310 320 330 340 350
YLLFTMIFVT LSIVITVFVL NVHYRTPTTH TMPTWVKAVF LNLLPRVMFM
360 370 380 390 400
TRPTSTEEDA PKTRNFYGAE LSNLNCFSRA DSKSCKEGYP CQDGTCGYCH
410 420 430 440 450
HRRVKISNFS ANLTRSSSSE SVDAVLSLSA LSPEIKEAIQ SVKYIAENMK
460 470 480 490
AQNVAKEIQD DWKYVAMVID RIFLWVFILV CILGTAGLFL QPLMARDDT
Length:499
Mass (Da):57,110
Last modified:June 1, 2002 - v1
Checksum:i721650E3F38D00FD
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q0VBK4Q0VBK4_MOUSE
Cholinergic receptor, nicotinic, al...
Chrna3 mCG_9678
504Annotation score:
A0A1L1SU72A0A1L1SU72_MOUSE
Neuronal acetylcholine receptor sub...
Chrna3
476Annotation score:

Sequence cautioni

The sequence BAC37909 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF472588 mRNA Translation: AAL84757.1
AY574262 mRNA Translation: AAS90358.1
AK051730 mRNA Translation: BAC34740.1
AK053497 mRNA Translation: BAC35404.1
AK080415 mRNA Translation: BAC37909.1 Different initiation.
RefSeqiNP_660111.2, NM_145129.2
UniGeneiMm.63569

Genome annotation databases

GeneIDi110834
KEGGimmu:110834
UCSCiuc009pry.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF472588 mRNA Translation: AAL84757.1
AY574262 mRNA Translation: AAS90358.1
AK051730 mRNA Translation: BAC34740.1
AK053497 mRNA Translation: BAC35404.1
AK080415 mRNA Translation: BAC37909.1 Different initiation.
RefSeqiNP_660111.2, NM_145129.2
UniGeneiMm.63569

3D structure databases

ProteinModelPortaliQ8R4G9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi225944, 2 interactors
ComplexPortaliCPX-188 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta2
CPX-189 Neuronal nicotinic acetylcholine receptor complex, alpha3-beta2
CPX-202 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta2-beta3
CPX-204 Neuronal nicotinic acetylcholine receptor complex, alpha3-beta4
CPX-209 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta4
CPX-212 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta4
STRINGi10090.ENSMUSP00000034851

Chemistry databases

ChEMBLiCHEMBL3885609

PTM databases

PhosphoSitePlusiQ8R4G9

Proteomic databases

MaxQBiQ8R4G9
PaxDbiQ8R4G9
PRIDEiQ8R4G9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi110834
KEGGimmu:110834
UCSCiuc009pry.1 mouse

Organism-specific databases

CTDi1136
MGIiMGI:87887 Chrna3

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
HOVERGENiHBG003756
InParanoidiQ8R4G9
KOiK04805
PhylomeDBiQ8R4G9

Miscellaneous databases

ChiTaRSiChrna3 mouse
PROiPR:Q8R4G9
SOURCEiSearch...

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACHA3_MOUSE
AccessioniPrimary (citable) accession number: Q8R4G9
Secondary accession number(s): Q8BV44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: June 1, 2002
Last modified: July 18, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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