UniProtKB - Q8R4E9 (CDT1_MOUSE)
DNA replication factor Cdt1
Cdt1
Functioni
Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments (By similarity).
Potential oncogene (PubMed:11850834).
By similarity3 PublicationsCaution
GO - Molecular functioni
- chromatin binding Source: MGI
- DNA binding Source: UniProtKB
- DNA polymerase binding Source: GO_Central
GO - Biological processi
- attachment of mitotic spindle microtubules to kinetochore Source: UniProtKB
- cell division Source: MGI
- chromosome segregation Source: UniProtKB
- deactivation of mitotic spindle assembly checkpoint Source: MGI
- DNA replication checkpoint signaling Source: UniProtKB
- DNA replication preinitiation complex assembly Source: MGI
- kinetochore organization Source: MGI
- mitotic cell cycle Source: MGI
- negative regulation of DNA-dependent DNA replication Source: Ensembl
- negative regulation of protein localization to kinetochore Source: MGI
- positive regulation of chromatin binding Source: MGI
- positive regulation of DNA-dependent DNA replication Source: MGI
- positive regulation of DNA replication Source: MGI
- positive regulation of protein-containing complex assembly Source: MGI
- positive regulation of protein localization to kinetochore Source: MGI
- regulation of chromosome organization Source: MGI
- regulation of DNA-dependent DNA replication initiation Source: UniProtKB
- regulation of DNA replication origin binding Source: MGI
- regulation of nuclear cell cycle DNA replication Source: MGI
- response to sorbitol Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, DNA replication |
Enzyme and pathway databases
Reactomei | R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication factor Cdt1Alternative name(s): Double parked homolog Short name: DUP Retroviral insertion site 2 protein |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914427, Cdt1 |
VEuPathDBi | HostDB:ENSMUSG00000006585 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- kinetochore By similarity
Note: Transiently localizes to kinetochores during prometaphase and metaphase.By similarity
Nucleus
- nuclear body Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- kinetochore Source: MGI
Keywords - Cellular componenti
Centromere, Chromosome, Kinetochore, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191620 | 1 – 557 | DNA replication factor Cdt1Add BLAST | 557 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 28 | Phosphothreonine; by MAPK8By similarity | 1 | |
Modified residuei | 30 | PhosphoserineBy similarity | 1 | |
Modified residuei | 107 | Phosphoserine; by MAPK8By similarity | 1 | |
Modified residuei | 392 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8R4E9 |
MaxQBi | Q8R4E9 |
PaxDbi | Q8R4E9 |
PeptideAtlasi | Q8R4E9 |
PRIDEi | Q8R4E9 |
ProteomicsDBi | 281303 |
PTM databases
iPTMneti | Q8R4E9 |
PhosphoSitePlusi | Q8R4E9 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000006585, Expressed in fetal liver hematopoietic progenitor cell and 262 other tissues |
Genevisiblei | Q8R4E9, MM |
Interactioni
Subunit structurei
Interacts with GMNN; inhibits binding of the MCM complex to origins of replication (PubMed:12192004).
Interacts with CDC6; are mutually dependent on one another for loading MCM complexes onto chromatin (By similarity).
Interacts with PCNA (By similarity).
Interacts with LRWD1 during G1 phase and during mitosis (By similarity).
Interacts with NDC80 subunit of the NDC80 complex; leading to kinetochore localization (By similarity).
Interacts with KAT7 (By similarity).
Interacts with ubiquitin-binding protein FAF1; the interaction is likely to promote CDT1 degradation (By similarity).
By similarity1 PublicationBinary interactionsi
GO - Molecular functioni
- DNA polymerase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 211995, 5 interactors |
IntActi | Q8R4E9, 5 interactors |
STRINGi | 10090.ENSMUSP00000006760 |
Miscellaneous databases
RNActi | Q8R4E9, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q8R4E9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8R4E9 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 20 – 113 | DisorderedSequence analysisAdd BLAST | 94 | |
Regioni | 163 – 203 | Interaction with GMNNBy similarityAdd BLAST | 41 | |
Regioni | 397 – 427 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 463 – 557 | Interaction with LRWD1By similarityAdd BLAST | 95 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1 – 25 | PIP-box K+4 motifBy similarityAdd BLAST | 25 | |
Motifi | 65 – 67 | Cyclin-binding motifBy similarity | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 81 – 104 | Pro residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 405 – 422 | Pro residuesSequence analysisAdd BLAST | 18 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4762, Eukaryota |
GeneTreei | ENSGT00390000012337 |
HOGENOMi | CLU_023373_1_0_1 |
InParanoidi | Q8R4E9 |
OMAi | DRVKEHH |
OrthoDBi | 648388at2759 |
PhylomeDBi | Q8R4E9 |
TreeFami | TF101159 |
Family and domain databases
CDDi | cd08767, Cdt1_c, 1 hit cd08674, Cdt1_m, 1 hit |
Gene3Di | 1.10.10.1420, 1 hit |
InterProi | View protein in InterPro IPR045173, Cdt1 IPR032054, Cdt1_C IPR038090, Cdt1_C_WH_dom_sf IPR014939, CDT1_Gemini-bd-like IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR28637, PTHR28637, 1 hit |
Pfami | View protein in Pfam PF08839, CDT1, 1 hit PF16679, CDT1_C, 1 hit |
SMARTi | View protein in SMART SM01075, CDT1, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAQSRVTDFY ACRRPGLTTP RAKSICLTPS PGGLVAPAFT RSSSRKRARP
60 70 80 90 100
PAEPGSDQPA PLARRRLRLP GLDSCPSSLP EPSSPAEPSP PADPSPPADP
110 120 130 140 150
GSPVCPSPVK RTKSTTVYVG QQPGKIPSED SVSELQSCLR RARKLGAQAR
160 170 180 190 200
ALRARVQENA VEPSTPDAKV PTEQPCVEKA PAYQRFHALA QPGLPGLVLP
210 220 230 240 250
YKYQVLVEMF RSMDTIVSML HNRSETVTFA KVKQGVQEMM RKRFEERNVG
260 270 280 290 300
QIKTVYPTSY RFRQECNVPT FKDSIKRSDY QLTIEPLLGQ EAGGATQLTA
310 320 330 340 350
TCLLQRRQVF RQNLVERVKE QHKVFLASLN PPMAVPDDQL TRWHPRFNVD
360 370 380 390 400
EVPDIEPAEL PQPPVTEKLT TAQEVLARAR SLMTPKMEKA LSNLALRSAE
410 420 430 440 450
PGSPGTSTPP LPATPPATPP AASPSALKGV SQALLERIRA KEVQKQLARM
460 470 480 490 500
TRCPEQELRL QRLERLPELA RVLRNVFVSE RKPALTMEVV CARMVDSCQT
510 520 530 540 550
ALSPGEMEKH LVLLAELLPD WLSLHRIRTD TYVKLDKAVD LAGLTARLAH
HVHAEGL
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | S → G in BAC33579 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 211 | R → H in AAL88446 (PubMed:11850834).Curated | 1 | |
Sequence conflicti | 258 | T → M in AAL88446 (PubMed:11850834).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF477481 mRNA Translation: AAL82630.1 AF477990 Genomic DNA Translation: AAL88446.1 AB086655 mRNA Translation: BAC22085.1 AK028287 mRNA Translation: BAC25859.1 AK049163 mRNA Translation: BAC33579.1 AK083015 mRNA Translation: BAC38731.1 Frameshift. BC024485 mRNA Translation: AAH24485.1 BC048076 mRNA Translation: AAH48076.1 |
CCDSi | CCDS22741.1 |
RefSeqi | NP_080290.3, NM_026014.3 |
Genome annotation databases
Ensembli | ENSMUST00000006760; ENSMUSP00000006760; ENSMUSG00000006585 |
GeneIDi | 67177 |
KEGGi | mmu:67177 |
UCSCi | uc009ntd.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF477481 mRNA Translation: AAL82630.1 AF477990 Genomic DNA Translation: AAL88446.1 AB086655 mRNA Translation: BAC22085.1 AK028287 mRNA Translation: BAC25859.1 AK049163 mRNA Translation: BAC33579.1 AK083015 mRNA Translation: BAC38731.1 Frameshift. BC024485 mRNA Translation: AAH24485.1 BC048076 mRNA Translation: AAH48076.1 |
CCDSi | CCDS22741.1 |
RefSeqi | NP_080290.3, NM_026014.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KLO | NMR | - | A | 420-557 | [»] | |
2RQQ | NMR | - | A | 450-557 | [»] | |
2ZXX | X-ray | 2.80 | C/F | 172-368 | [»] | |
3A4C | X-ray | 1.89 | A | 452-557 | [»] | |
SMRi | Q8R4E9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 211995, 5 interactors |
IntActi | Q8R4E9, 5 interactors |
STRINGi | 10090.ENSMUSP00000006760 |
PTM databases
iPTMneti | Q8R4E9 |
PhosphoSitePlusi | Q8R4E9 |
Proteomic databases
EPDi | Q8R4E9 |
MaxQBi | Q8R4E9 |
PaxDbi | Q8R4E9 |
PeptideAtlasi | Q8R4E9 |
PRIDEi | Q8R4E9 |
ProteomicsDBi | 281303 |
Protocols and materials databases
Antibodypediai | 1905, 216 antibodies from 36 providers |
DNASUi | 67177 |
Genome annotation databases
Ensembli | ENSMUST00000006760; ENSMUSP00000006760; ENSMUSG00000006585 |
GeneIDi | 67177 |
KEGGi | mmu:67177 |
UCSCi | uc009ntd.1, mouse |
Organism-specific databases
CTDi | 81620 |
MGIi | MGI:1914427, Cdt1 |
VEuPathDBi | HostDB:ENSMUSG00000006585 |
Phylogenomic databases
eggNOGi | KOG4762, Eukaryota |
GeneTreei | ENSGT00390000012337 |
HOGENOMi | CLU_023373_1_0_1 |
InParanoidi | Q8R4E9 |
OMAi | DRVKEHH |
OrthoDBi | 648388at2759 |
PhylomeDBi | Q8R4E9 |
TreeFami | TF101159 |
Enzyme and pathway databases
Reactomei | R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex |
Miscellaneous databases
BioGRID-ORCSi | 67177, 16 hits in 67 CRISPR screens |
ChiTaRSi | Cdt1, mouse |
EvolutionaryTracei | Q8R4E9 |
PROi | PR:Q8R4E9 |
RNActi | Q8R4E9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000006585, Expressed in fetal liver hematopoietic progenitor cell and 262 other tissues |
Genevisiblei | Q8R4E9, MM |
Family and domain databases
CDDi | cd08767, Cdt1_c, 1 hit cd08674, Cdt1_m, 1 hit |
Gene3Di | 1.10.10.1420, 1 hit |
InterProi | View protein in InterPro IPR045173, Cdt1 IPR032054, Cdt1_C IPR038090, Cdt1_C_WH_dom_sf IPR014939, CDT1_Gemini-bd-like IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR28637, PTHR28637, 1 hit |
Pfami | View protein in Pfam PF08839, CDT1, 1 hit PF16679, CDT1_C, 1 hit |
SMARTi | View protein in SMART SM01075, CDT1, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CDT1_MOUSE | |
Accessioni | Q8R4E9Primary (citable) accession number: Q8R4E9 Secondary accession number(s): Q8BUQ1 Q8R4E8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 7, 2004 |
Last sequence update: | June 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families