UniProtKB - Q8R424 (STABP_RAT)
Protein
STAM-binding protein
Gene
Stambp
Organism
Rattus norvegicus (Rat)
Status
Functioni
Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7 (By similarity). Involved in the ubiquitin-dependent sorting and trafficking of receptors from endosomes to lysosome. Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization. Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways (By similarity).By similarity
Cofactori
Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
Activity regulationi
Inhibited by N-ethylmaleimide.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 280 | Indirect zinc-bindingBy similarity | 1 | |
Metal bindingi | 335 | Zinc 1; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 337 | Zinc 1; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 348 | Zinc 1; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 350 | Zinc 2By similarity | 1 | |
Metal bindingi | 390 | Zinc 2By similarity | 1 | |
Metal bindingi | 396 | Zinc 2By similarity | 1 | |
Metal bindingi | 398 | Zinc 2By similarity | 1 |
GO - Molecular functioni
- isopeptidase activity Source: InterPro
- Lys63-specific deubiquitinase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- metallopeptidase activity Source: UniProtKB-KW
- protein domain specific binding Source: RGD
- thiol-dependent ubiquitin-specific protease activity Source: RGD
GO - Biological processi
- mitotic cytokinesis Source: RGD
- negative regulation of neuron apoptotic process Source: RGD
- negative regulation of phosphatidylinositol 3-kinase signaling Source: RGD
- negative regulation of Ras protein signal transduction Source: RGD
- protein deubiquitination Source: RGD
- protein K63-linked deubiquitination Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-5689901, Metalloprotease DUBs |
Protein family/group databases
MEROPSi | M67.006 |
Names & Taxonomyi
Protein namesi | Recommended name: STAM-binding protein (EC:3.4.19.-)Alternative name(s): Associated molecule with the SH3 domain of STAM |
Gene namesi | Name:Stambp Synonyms:Amsh |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619963, Stambp |
Subcellular locationi
Nucleus
- Nucleus By similarity
Endosome
- Endosome By similarity
Other locations
- Membrane By similarity; Peripheral membrane protein By similarity
- Cytoplasm By similarity
Cytosol
- cytosol Source: Ensembl
Endosome
- endosome Source: GO_Central
Nucleus
- nucleoplasm Source: Ensembl
Plasma Membrane
- cleavage furrow Source: RGD
Other locations
- membrane Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Endosome, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194871 | 1 – 424 | STAM-binding proteinAdd BLAST | 424 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 48 | PhosphoserineBy similarity | 1 | |
Modified residuei | 243 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated after BMP type I receptor activation.By similarity
Ubiquitinated by SMURF2 in the presence of RNF11.By similarity
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q8R424 |
PRIDEi | Q8R424 |
PTM databases
iPTMneti | Q8R424 |
PhosphoSitePlusi | Q8R424 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000012227, Expressed in testis and 22 other tissues |
Genevisiblei | Q8R424, RN |
Interactioni
Subunit structurei
Interacts with STAM.
Interacts with SMAD6 and SMAD7.
Interacts with CHMP3; the interaction appears to relieve the autoinhibition of CHMP3 (By similarity).
Interacts with SMURF2 and RNF11; this interaction promotes ubiquitination (By similarity).
By similarityGO - Molecular functioni
- protein domain specific binding Source: RGD
Protein-protein interaction databases
IntActi | Q8R424, 2 interactors |
STRINGi | 10116.ENSRNOP00000014708 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 257 – 388 | MPNPROSITE-ProRule annotationAdd BLAST | 132 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 127 | Interaction with CHMP3By similarityAdd BLAST | 127 | |
Regioni | 227 – 231 | Interaction with STAM1By similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 335 – 348 | JAMM motifPROSITE-ProRule annotationAdd BLAST | 14 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 73 – 127 | Lys-richAdd BLAST | 55 | |
Compositional biasi | 139 – 172 | Gln-richAdd BLAST | 34 |
Domaini
The JAMM motif is essential for the protease activity.By similarity
Sequence similaritiesi
Belongs to the peptidase M67C family.Curated
Phylogenomic databases
eggNOGi | KOG2880, Eukaryota |
GeneTreei | ENSGT00940000153710 |
HOGENOMi | CLU_023304_0_1_1 |
InParanoidi | Q8R424 |
OMAi | CNTMHEE |
OrthoDBi | 411229at2759 |
PhylomeDBi | Q8R424 |
TreeFami | TF323215 |
Family and domain databases
CDDi | cd08066, MPN_AMSH_like, 1 hit |
InterProi | View protein in InterPro IPR000555, JAMM/MPN+_dom IPR037518, MPN IPR044098, STAMBP/STALP-like_MPN IPR015063, USP8_dimer |
Pfami | View protein in Pfam PF01398, JAB, 1 hit PF08969, USP8_dimer, 1 hit |
SMARTi | View protein in SMART SM00232, JAB_MPN, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8R424-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDHADVSLP PQDRVRILSQ LGSAVELNED IPPRRYFRSG VEIIRMASIY
60 70 80 90 100
SEEGNIEHAF ILYNKYITLF IEKLPKHRDY KSAIIPEKKD AVKKLKNVAF
110 120 130 140 150
PKAEELKTEL LKRYTKEYEQ YKERKKKEEE ELARNIAIQQ ELEKEKQRVA
160 170 180 190 200
QQKQKQLEQE QFHAFEKMIQ KQELEKERLK IVQEFGKVDP GPCGPLLPDL
210 220 230 240 250
EKPCVDVAPS SPFSPTQTSD CNTTLRPAKP PVVDRSLKPG ALSVIENVPT
260 270 280 290 300
IEGLRHIVVP RNLCSEFLQL ASANTAKGIE TCGVLCGKLM RNEFTITHVL
310 320 330 340 350
IPRQNGGPDY CHTENEEEIF FMQDDLGLLT LGWIHTHPTQ TAFLSSVDLH
360 370 380 390 400
THCSYQMMLP ESIAIVCSPK FQETGFFKLT DYGLQEISTC RQKGFHPHGR
410 420
DPPLFCDCSH VTVKDRIVTI TDLR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY083159 mRNA Translation: AAL92520.1 BC061711 mRNA Translation: AAH61711.1 |
RefSeqi | NP_612540.1, NM_138531.2 XP_006236788.1, XM_006236726.3 XP_006236789.1, XM_006236727.3 XP_008761267.1, XM_008763045.2 XP_008761268.1, XM_008763046.2 XP_008761269.1, XM_008763047.2 XP_008761270.1, XM_008763048.2 |
Genome annotation databases
Ensembli | ENSRNOT00000014708; ENSRNOP00000014708; ENSRNOG00000012227 ENSRNOT00000086968; ENSRNOP00000069824; ENSRNOG00000012227 |
GeneIDi | 171565 |
KEGGi | rno:171565 |
UCSCi | RGD:619963, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY083159 mRNA Translation: AAL92520.1 BC061711 mRNA Translation: AAH61711.1 |
RefSeqi | NP_612540.1, NM_138531.2 XP_006236788.1, XM_006236726.3 XP_006236789.1, XM_006236727.3 XP_008761267.1, XM_008763045.2 XP_008761268.1, XM_008763046.2 XP_008761269.1, XM_008763047.2 XP_008761270.1, XM_008763048.2 |
3D structure databases
SMRi | Q8R424 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q8R424, 2 interactors |
STRINGi | 10116.ENSRNOP00000014708 |
Protein family/group databases
MEROPSi | M67.006 |
PTM databases
iPTMneti | Q8R424 |
PhosphoSitePlusi | Q8R424 |
Proteomic databases
PaxDbi | Q8R424 |
PRIDEi | Q8R424 |
Genome annotation databases
Ensembli | ENSRNOT00000014708; ENSRNOP00000014708; ENSRNOG00000012227 ENSRNOT00000086968; ENSRNOP00000069824; ENSRNOG00000012227 |
GeneIDi | 171565 |
KEGGi | rno:171565 |
UCSCi | RGD:619963, rat |
Organism-specific databases
CTDi | 10617 |
RGDi | 619963, Stambp |
Phylogenomic databases
eggNOGi | KOG2880, Eukaryota |
GeneTreei | ENSGT00940000153710 |
HOGENOMi | CLU_023304_0_1_1 |
InParanoidi | Q8R424 |
OMAi | CNTMHEE |
OrthoDBi | 411229at2759 |
PhylomeDBi | Q8R424 |
TreeFami | TF323215 |
Enzyme and pathway databases
Reactomei | R-RNO-5689901, Metalloprotease DUBs |
Miscellaneous databases
PROi | PR:Q8R424 |
Gene expression databases
Bgeei | ENSRNOG00000012227, Expressed in testis and 22 other tissues |
Genevisiblei | Q8R424, RN |
Family and domain databases
CDDi | cd08066, MPN_AMSH_like, 1 hit |
InterProi | View protein in InterPro IPR000555, JAMM/MPN+_dom IPR037518, MPN IPR044098, STAMBP/STALP-like_MPN IPR015063, USP8_dimer |
Pfami | View protein in Pfam PF01398, JAB, 1 hit PF08969, USP8_dimer, 1 hit |
SMARTi | View protein in SMART SM00232, JAB_MPN, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | STABP_RAT | |
Accessioni | Q8R424Primary (citable) accession number: Q8R424 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries