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Protein

tRNA-splicing endonuclease subunit Sen15

Gene

Tsen15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).By similarity

Caution

Although only weakly related to the S.cerevisiae SEN15 protein, it probably displays the same function within the tRNA splicing endonuclease complex.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen15
Alternative name(s):
tRNA-intron endonuclease Sen15
Gene namesi
Name:Tsen15
Synonyms:Sen15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1913887 Tsen15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940241 – 168tRNA-splicing endonuclease subunit Sen15Add BLAST168

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1
Modified residuei165PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8R3W5
PaxDbiQ8R3W5
PeptideAtlasiQ8R3W5
PRIDEiQ8R3W5

PTM databases

PhosphoSitePlusiQ8R3W5

Interactioni

Subunit structurei

Homodimer. tRNA splicing endonuclease is a heterotetramer composed of TSEN2, TSEN15, TSEN34/LENG5 and TSEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3' end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi211612, 1 interactor
STRINGi10090.ENSMUSP00000015124

Structurei

3D structure databases

ProteinModelPortaliQ8R3W5
SMRiQ8R3W5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SEN15 family.Curated

Phylogenomic databases

eggNOGiENOG410IYHQ Eukaryota
ENOG41120H2 LUCA
HOGENOMiHOG000290172
HOVERGENiHBG058504
InParanoidiQ8R3W5
KOiK15324
PhylomeDBiQ8R3W5

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR018593 tRNA-endonuc_su_Sen15
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
PfamiView protein in Pfam
PF09631 Sen15, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8R3W5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEERSDSEPT PGCSGPGPAP VRDGGGAHTW APEDAWMGTH PKYLEMMELD
60 70 80 90 100
IGDATQVYIA FLVYLDLMES KSWHEVNCVG IPELQLICLL GTEIEGEGLQ
110 120 130 140 150
TVVPTPISAS LSHNRIREIL KASRKLQGDP ELPMSFTLAI VESDSTIVYY
160
KLTDGFMLPD PQNISLRR
Length:168
Mass (Da):18,530
Last modified:June 1, 2002 - v1
Checksum:i99D8E6E14C2FCAA0
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3X8S8G3X8S8_MOUSE
MCG14499
Tsen15 mCG_14499
168Annotation score:
E0CY96E0CY96_MOUSE
tRNA-splicing endonuclease subunit ...
Tsen15
128Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5 – 8SDSE → TSSK in BAB30855 (PubMed:16141072).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017650 mRNA Translation: BAB30855.1
BC024342 mRNA Translation: AAH24342.1
BC145701 mRNA Translation: AAI45702.1
CCDSiCCDS15363.1
RefSeqiNP_079953.2, NM_025677.3
UniGeneiMm.379120

Genome annotation databases

GeneIDi66637
KEGGimmu:66637

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017650 mRNA Translation: BAB30855.1
BC024342 mRNA Translation: AAH24342.1
BC145701 mRNA Translation: AAI45702.1
CCDSiCCDS15363.1
RefSeqiNP_079953.2, NM_025677.3
UniGeneiMm.379120

3D structure databases

ProteinModelPortaliQ8R3W5
SMRiQ8R3W5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211612, 1 interactor
STRINGi10090.ENSMUSP00000015124

PTM databases

PhosphoSitePlusiQ8R3W5

Proteomic databases

MaxQBiQ8R3W5
PaxDbiQ8R3W5
PeptideAtlasiQ8R3W5
PRIDEiQ8R3W5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi66637
KEGGimmu:66637

Organism-specific databases

CTDi116461
MGIiMGI:1913887 Tsen15

Phylogenomic databases

eggNOGiENOG410IYHQ Eukaryota
ENOG41120H2 LUCA
HOGENOMiHOG000290172
HOVERGENiHBG058504
InParanoidiQ8R3W5
KOiK15324
PhylomeDBiQ8R3W5

Miscellaneous databases

PROiPR:Q8R3W5
SOURCEiSearch...

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR018593 tRNA-endonuc_su_Sen15
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
PfamiView protein in Pfam
PF09631 Sen15, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSEN15_MOUSE
AccessioniPrimary (citable) accession number: Q8R3W5
Secondary accession number(s): A6H601, Q9CQW8, Q9CYI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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