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Protein

Store-operated calcium entry-associated regulatory factor

Gene

Saraf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negative regulator of store-operated Ca2+ entry (SOCE) involved in protecting cells from Ca2+ overfilling. In response to cytosolic Ca2+ elevation after endoplasmic reticulum Ca2+ refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca2+ levels, and thus preventing the overload of the cell with excessive Ca2+ ions (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processCalcium transport, Ion transport, Transport
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Store-operated calcium entry-associated regulatory factor
Short name:
SARAF
Short name:
SOCE-associated regulatory factor
Alternative name(s):
Transmembrane protein 66
Gene namesi
Name:Saraf
Synonyms:Tmem66
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1915137 Saraf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 168LumenalSequence analysisAdd BLAST137
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 334CytoplasmicSequence analysisAdd BLAST145

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000004548632 – 334Store-operated calcium entry-associated regulatory factorAdd BLAST303

Proteomic databases

EPDiQ8R3Q0
MaxQBiQ8R3Q0
PaxDbiQ8R3Q0
PeptideAtlasiQ8R3Q0
PRIDEiQ8R3Q0

Expressioni

Gene expression databases

CleanExiMM_TMEM66

Interactioni

Subunit structurei

Interacts with STIM1.By similarity

Protein-protein interaction databases

IntActiQ8R3Q0, 1 interactor
STRINGi10090.ENSMUSP00000033933

Family & Domainsi

Domaini

The cytoplasmic C-terminal region mediates interaction with STIM1, while the N-terminal lumenal region mediates regulation of SOCE activity.By similarity

Sequence similaritiesi

Belongs to the SARAF family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IV9Z Eukaryota
ENOG4111IKY LUCA
HOGENOMiHOG000154648
HOVERGENiHBG059843
InParanoidiQ8R3Q0
PhylomeDBiQ8R3Q0
TreeFamiTF314811

Family and domain databases

InterProiView protein in InterPro
IPR009567 SARAF
PANTHERiPTHR15929 PTHR15929, 1 hit
PfamiView protein in Pfam
PF06682 SARAF, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8R3Q0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVAAVGRPR ALRCPLLLLL SLLLVAGPAL GWNDPDRILL RDVKALTLYS
60 70 80 90 100
DRYTTSRRLD PIPQLKCVGG TAGCEAYTPR VIQCQNKGWD GYDVQWECKT
110 120 130 140 150
DLDIAYKFGK TVVSCEGYES SEDQYVLRGS CGLEYNLDYT ELGLKKLKES
160 170 180 190 200
GKHQGFSDYY HKLYSSDSCG FITIAVLFVL AFAVYKLFLS DGQGSPPPYS
210 220 230 240 250
EHPPYSEHSQ RFASAAGAPP PGFKSEFTGP QNTGYGASSG FGSAFGGQGY
260 270 280 290 300
GSSGPGFWSG LGAGGLLGYL FGSNRAATPF SDSWYHPAYP PSHSGAWNSR
310 320 330
AYSPLGGGAG SYCASSNADS RTRTASGYGG TRRR
Length:334
Mass (Da):35,856
Last modified:January 10, 2006 - v2
Checksum:iEA12FE138052BCC4
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J0D1A0A0R4J0D1_MOUSE
Store-operated calcium entry-associ...
Saraf
363Annotation score:

Sequence cautioni

The sequence AAH13497 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH22616 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH24888 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB25254 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC04255 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC40531 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE37240 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12L → V in BAC04255 (PubMed:16141072).Curated1
Sequence conflicti12L → V in BAC40531 (PubMed:16141072).Curated1
Sequence conflicti12L → V in AAH24888 (PubMed:15489334).Curated1
Sequence conflicti117G → V in AAH22616 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK093942 mRNA Translation: BAC04255.1 Different initiation.
AK007787 mRNA Translation: BAB25254.1 Different initiation.
AK075744 mRNA Translation: BAC35924.1
AK088728 mRNA Translation: BAC40531.1 Different initiation.
AK163218 mRNA Translation: BAE37240.1 Different initiation.
BC013497 mRNA Translation: AAH13497.1 Different initiation.
BC022616 mRNA Translation: AAH22616.1 Different initiation.
BC024888 mRNA Translation: AAH24888.1 Different initiation.
RefSeqiNP_080708.3, NM_026432.3
UniGeneiMm.290353

Genome annotation databases

GeneIDi67887
KEGGimmu:67887
UCSCiuc012gci.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK093942 mRNA Translation: BAC04255.1 Different initiation.
AK007787 mRNA Translation: BAB25254.1 Different initiation.
AK075744 mRNA Translation: BAC35924.1
AK088728 mRNA Translation: BAC40531.1 Different initiation.
AK163218 mRNA Translation: BAE37240.1 Different initiation.
BC013497 mRNA Translation: AAH13497.1 Different initiation.
BC022616 mRNA Translation: AAH22616.1 Different initiation.
BC024888 mRNA Translation: AAH24888.1 Different initiation.
RefSeqiNP_080708.3, NM_026432.3
UniGeneiMm.290353

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R3Q0, 1 interactor
STRINGi10090.ENSMUSP00000033933

Proteomic databases

EPDiQ8R3Q0
MaxQBiQ8R3Q0
PaxDbiQ8R3Q0
PeptideAtlasiQ8R3Q0
PRIDEiQ8R3Q0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi67887
KEGGimmu:67887
UCSCiuc012gci.1 mouse

Organism-specific databases

CTDi51669
MGIiMGI:1915137 Saraf

Phylogenomic databases

eggNOGiENOG410IV9Z Eukaryota
ENOG4111IKY LUCA
HOGENOMiHOG000154648
HOVERGENiHBG059843
InParanoidiQ8R3Q0
PhylomeDBiQ8R3Q0
TreeFamiTF314811

Miscellaneous databases

ChiTaRSiSaraf mouse
PROiPR:Q8R3Q0
SOURCEiSearch...

Gene expression databases

CleanExiMM_TMEM66

Family and domain databases

InterProiView protein in InterPro
IPR009567 SARAF
PANTHERiPTHR15929 PTHR15929, 1 hit
PfamiView protein in Pfam
PF06682 SARAF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSARAF_MOUSE
AccessioniPrimary (citable) accession number: Q8R3Q0
Secondary accession number(s): Q8C2F1
, Q8C6G7, Q8N9S4, Q8R233, Q9D8R1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: February 28, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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