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Protein

THO complex subunit 1

Gene

Thoc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation (By similarity).By similarity
Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1 (By similarity). Essential for early embryonic development. Required for normal gene expression during postnatal testis development.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, RNA-binding
Biological processApoptosis, mRNA processing, mRNA splicing, mRNA transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-72187 mRNA 3'-end processing

Names & Taxonomyi

Protein namesi
Recommended name:
THO complex subunit 1
Short name:
Tho1
Alternative name(s):
Nuclear matrix protein p84
Gene namesi
Name:Thoc1
Synonyms:Hpr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1919668 Thoc1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show early embryonic lethality and severely diminished fertility.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000725211 – 657THO complex subunit 1Add BLAST657

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei4PhosphothreonineBy similarity1
Cross-linki31Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei133N6-acetyllysineBy similarity1
Modified residuei300N6-acetyllysineBy similarity1
Cross-linki408Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei537PhosphoserineBy similarity1
Modified residuei542PhosphothreonineBy similarity1
Modified residuei560PhosphoserineCombined sources1
Cross-linki580Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki595Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki595Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity

Post-translational modificationi

Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass.By similarity
Polyubiquitinated, leading to proteasomal degradation; probably involves NEDD4.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8R3N6
MaxQBiQ8R3N6
PaxDbiQ8R3N6
PeptideAtlasiQ8R3N6
PRIDEiQ8R3N6

PTM databases

iPTMnetiQ8R3N6
PhosphoSitePlusiQ8R3N6
SwissPalmiQ8R3N6

Expressioni

Developmental stagei

Widely expressed during embryonic development.1 Publication

Gene expression databases

BgeeiENSMUSG00000024287 Expressed in 272 organ(s), highest expression level in skin of back
CleanExiMM_THOC1
GenevisibleiQ8R3N6 MM

Interactioni

Subunit structurei

Component of the THO complex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7; together with at least ALYREF/THOC4, DDX39B, SARNP/CIP29 and CHTOP, THO forms the transcription/export (TREX) complex which seems to have a dynamic structure involving ATP-dependent remodeling. Binds to the hypophosphorylated form of RB1. Interacts with THOC2, DDX39B and RNA polymerase II (By similarity). Interacts with THOC5 (PubMed:16909111). Interacts with LUZP4 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi230364, 1 interactor
IntActiQ8R3N6, 3 interactors
MINTiQ8R3N6
STRINGi10090.ENSMUSP00000025137

Structurei

3D structure databases

ProteinModelPortaliQ8R3N6
SMRiQ8R3N6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini570 – 653DeathPROSITE-ProRule annotationAdd BLAST84

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi414 – 430Nuclear localization signalBy similarityAdd BLAST17

Domaini

An intact death domain is needed for apoptosis.By similarity

Phylogenomic databases

eggNOGiKOG2491 Eukaryota
ENOG410XQEX LUCA
GeneTreeiENSGT00390000016232
HOGENOMiHOG000008123
HOVERGENiHBG060294
InParanoidiQ8R3N6
KOiK12878
OMAiVSEFNLD
OrthoDBiEOG091G03G3
PhylomeDBiQ8R3N6
TreeFamiTF314796

Family and domain databases

InterProiView protein in InterPro
IPR011029 DEATH-like_dom_sf
IPR000488 Death_domain
IPR021861 THO_THOC1
PANTHERiPTHR13265 PTHR13265, 1 hit
PfamiView protein in Pfam
PF00531 Death, 1 hit
PF11957 efThoc1, 1 hit
SMARTiView protein in SMART
SM00005 DEATH, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
PROSITEiView protein in PROSITE
PS50017 DEATH_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R3N6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSPTPALFSL PEARTRFTKS TREALNNKNI KPLLTAFSQL PGSENEKKCT
60 70 80 90 100
LDQAFRGVLE EEIINHSACE NVLAIISLAI GGVTESVCTA STPFVLLGDV
110 120 130 140 150
LDCLPLDQCD TIFTFVEKNV ATWKSNTFYS AGKNYLLRMC NDLLRRLSKS
160 170 180 190 200
QNTVFCGRIQ LFLARLFPLS EKSGLNLQSQ FNLENVTVFN TNEQESTLGQ
210 220 230 240 250
KHTEDREEGM DVEEGEMGDD EAPTTCSIPI DYNLYRKFWS LQDYFRNPVQ
260 270 280 290 300
CYEKISWKTF LKYSEEVLAV FKSYKLDDTQ ASRKKMEELK TGGEHVYFAK
310 320 330 340 350
FLTSEKLMDL QLSDSNFRRH ILLQYLILFQ YLKGQVKFKS SNYVLTDEQS
360 370 380 390 400
LWIEDTTKSV YQLLSENPPD GERFSKMVEH ILNTEENWNS WKNEGCPSFV
410 420 430 440 450
KERASDTKPT RVVRKRAAPE DFLGKGPNKK ILIGNEELTR LWNLCPDNME
460 470 480 490 500
ACKSETREYM PTLEEFFEEA IEQADPENMV ESEYKAVNNS NYGWRALRLL
510 520 530 540 550
ARRSPHFFQP TNQQFKSLPE YLENMVIKLA KELPPPSEEI KTGEDEDEED
560 570 580 590 600
NDALLKENES PDVRRDKPIT GEQIESFANK LGEQWKILAP YLEIKDSDIR
610 620 630 640 650
QIECDSEDMK MRAKQLLVAW QDQEGVHATT DNLISALNKS GLSDLAESLT

NDTETNS
Length:657
Mass (Da):75,436
Last modified:June 1, 2002 - v1
Checksum:iE4235E395B5A82BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031785 mRNA Translation: BAC27548.1
AK032200 mRNA Translation: BAC27754.1
AK042867 mRNA Translation: BAC31387.2
BC024951 mRNA Translation: AAH24951.1
CCDSiCCDS37733.1
RefSeqiNP_705780.1, NM_153552.3
UniGeneiMm.219648
Mm.329277

Genome annotation databases

EnsembliENSMUST00000025137; ENSMUSP00000025137; ENSMUSG00000024287
GeneIDi225160
KEGGimmu:225160
UCSCiuc008eal.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031785 mRNA Translation: BAC27548.1
AK032200 mRNA Translation: BAC27754.1
AK042867 mRNA Translation: BAC31387.2
BC024951 mRNA Translation: AAH24951.1
CCDSiCCDS37733.1
RefSeqiNP_705780.1, NM_153552.3
UniGeneiMm.219648
Mm.329277

3D structure databases

ProteinModelPortaliQ8R3N6
SMRiQ8R3N6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230364, 1 interactor
IntActiQ8R3N6, 3 interactors
MINTiQ8R3N6
STRINGi10090.ENSMUSP00000025137

PTM databases

iPTMnetiQ8R3N6
PhosphoSitePlusiQ8R3N6
SwissPalmiQ8R3N6

Proteomic databases

EPDiQ8R3N6
MaxQBiQ8R3N6
PaxDbiQ8R3N6
PeptideAtlasiQ8R3N6
PRIDEiQ8R3N6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025137; ENSMUSP00000025137; ENSMUSG00000024287
GeneIDi225160
KEGGimmu:225160
UCSCiuc008eal.1 mouse

Organism-specific databases

CTDi9984
MGIiMGI:1919668 Thoc1

Phylogenomic databases

eggNOGiKOG2491 Eukaryota
ENOG410XQEX LUCA
GeneTreeiENSGT00390000016232
HOGENOMiHOG000008123
HOVERGENiHBG060294
InParanoidiQ8R3N6
KOiK12878
OMAiVSEFNLD
OrthoDBiEOG091G03G3
PhylomeDBiQ8R3N6
TreeFamiTF314796

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-72187 mRNA 3'-end processing

Miscellaneous databases

PROiPR:Q8R3N6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024287 Expressed in 272 organ(s), highest expression level in skin of back
CleanExiMM_THOC1
GenevisibleiQ8R3N6 MM

Family and domain databases

InterProiView protein in InterPro
IPR011029 DEATH-like_dom_sf
IPR000488 Death_domain
IPR021861 THO_THOC1
PANTHERiPTHR13265 PTHR13265, 1 hit
PfamiView protein in Pfam
PF00531 Death, 1 hit
PF11957 efThoc1, 1 hit
SMARTiView protein in SMART
SM00005 DEATH, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
PROSITEiView protein in PROSITE
PS50017 DEATH_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTHOC1_MOUSE
AccessioniPrimary (citable) accession number: Q8R3N6
Secondary accession number(s): Q8BWD5, Q8BXY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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