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Protein

WD repeat domain phosphoinositide-interacting protein 1

Gene

Wipi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in autophagy and in particular starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions upstream of the ATG12-ATG5-ATG16L1 complex and LC3, and downstream of the ULK1 and PI3-kinase complexes. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat domain phosphoinositide-interacting protein 1
Short name:
WIPI-1
Alternative name(s):
WD40 repeat protein interacting with phosphoinositides of 49 kDa
Short name:
WIPI 49 kDa
Gene namesi
Name:Wipi1
Synonyms:D11Ertd498e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1261864 Wipi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000514381 – 446WD repeat domain phosphoinositide-interacting protein 1Add BLAST446

Proteomic databases

PaxDbiQ8R3E3
PeptideAtlasiQ8R3E3
PRIDEiQ8R3E3

PTM databases

iPTMnetiQ8R3E3
PhosphoSitePlusiQ8R3E3

Expressioni

Gene expression databases

BgeeiENSMUSG00000041895 Expressed in 278 organ(s), highest expression level in membranous labyrinth
CleanExiMM_WIPI1
ExpressionAtlasiQ8R3E3 baseline and differential
GenevisibleiQ8R3E3 MM

Interactioni

Subunit structurei

Interacts with androgen receptor (AR) and the estrogen receptors ESR1 and ESR2. Binds PtdIns3P and to a lesser extent, PtdIns3,5P2 and PtdIns5P in vitro. Interaction with PtdIns3P is required for recruitment to membranes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099349

Structurei

3D structure databases

ProteinModelPortaliQ8R3E3
SMRiQ8R3E3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati184 – 224WD 1Add BLAST41
Repeati230 – 269WD 2Add BLAST40
Repeati312 – 351WD 3Add BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi131 – 136Nuclear receptor interactionBy similarity6

Domaini

The N-terminus might form a beta-propeller domain involved in specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2), leading to the association of the protein to the membrane. Association to the membrane can also occur through binding to phosphatidylinositol 3-monophosphate (PI3P).

Sequence similaritiesi

Belongs to the WD repeat SVP1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2110 Eukaryota
ENOG410XP6Y LUCA
GeneTreeiENSGT00730000110845
HOGENOMiHOG000217544
HOVERGENiHBG056639
InParanoidiQ8R3E3
KOiK17908
OMAiKMFMAAS
OrthoDBiEOG091G08ZS
PhylomeDBiQ8R3E3
TreeFamiTF314879

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR036322 WD40_repeat_dom_sf
IPR032909 WIPI1
PANTHERiPTHR11227:SF23 PTHR11227:SF23, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 3 hits
SUPFAMiSSF50978 SSF50978, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8R3E3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEAEAADAPP GRVEAALSCF SFNQDCTSLA IGTKAGYKLF SLSSVEQLDQ
60 70 80 90 100
VHGSNEIPDV YIVERLFSSS LVVVVSHTKP RQMNVYHFKK GTEICNYSYS
110 120 130 140 150
SNILSIRLNR QRLLVCLEES IYIHNIKDMK LLKTVLDIPS NPTGLCALSI
160 170 180 190 200
NHSNSYLAYP GSQSTGEIVL YDGNSLKTVC TIAAHEGTLA AITFNSSGSK
210 220 230 240 250
LASASEKGTV IRVFSVPEGQ KLYEFRRGMK RYVTISSLVF SMDSQFLCAS
260 270 280 290 300
SNTETVHIFK MEHLTDSRPE EPSTWSGYMG KMFMAATNYL PAQVSDMMNQ
310 320 330 340 350
DRAFATGRLN FSGQKNICTL STIQKLPRLL VASSDGHLYI YNLDPQDGGE
360 370 380 390 400
CVLIKTHSLL SSGTTEENKE NDLRPSLPPS YAATVARPST SAASTVPGYS
410 420 430 440
EDGGALRGEV IPEHEFATGP VCLDDENEFP PIILCRGSQK GKTKQS
Length:446
Mass (Da):48,758
Last modified:June 1, 2002 - v1
Checksum:i9F7FA1CB802E5344
GO
Isoform 2 (identifier: Q8R3E3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     432-446: IILCRGSQKGKTKQS → VSIRNP

Show »
Length:437
Mass (Da):47,796
Checksum:iA2E0FD91A644018D
GO
Isoform 3 (identifier: Q8R3E3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     267-270: SRPE → RCRT
     271-446: Missing.

Note: No experimental confirmation available.
Show »
Length:270
Mass (Da):29,819
Checksum:i4F3C3E0EF20A2C5B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AT64B1AT64_MOUSE
WD repeat domain phosphoinositide-i...
Wipi1
295Annotation score:

Sequence cautioni

The sequence AAH24811 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH24883 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016967267 – 270SRPE → RCRT in isoform 3. 1 Publication4
Alternative sequenceiVSP_016968271 – 446Missing in isoform 3. 1 PublicationAdd BLAST176
Alternative sequenceiVSP_016969432 – 446IILCR…KTKQS → VSIRNP in isoform 2. 2 PublicationsAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031672 mRNA Translation: BAC27504.1
AK034907 mRNA Translation: BAC28878.1
AK050495 mRNA Translation: BAE20673.1
AK159608 mRNA Translation: BAE35227.1
AK170563 mRNA Translation: BAE41884.1
BC024811 mRNA Translation: AAH24811.1 Different initiation.
BC024883 mRNA Translation: AAH24883.1 Different initiation.
BC025560 mRNA Translation: AAH25560.1
CCDSiCCDS25582.1 [Q8R3E3-1]
RefSeqiNP_666052.1, NM_145940.2 [Q8R3E3-1]
UniGeneiMm.35817

Genome annotation databases

EnsembliENSMUST00000047186; ENSMUSP00000038635; ENSMUSG00000041895 [Q8R3E3-2]
ENSMUST00000103060; ENSMUSP00000099349; ENSMUSG00000041895 [Q8R3E3-1]
GeneIDi52639
KEGGimmu:52639
UCSCiuc007mcq.1 mouse [Q8R3E3-1]
uc007mcr.1 mouse [Q8R3E3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031672 mRNA Translation: BAC27504.1
AK034907 mRNA Translation: BAC28878.1
AK050495 mRNA Translation: BAE20673.1
AK159608 mRNA Translation: BAE35227.1
AK170563 mRNA Translation: BAE41884.1
BC024811 mRNA Translation: AAH24811.1 Different initiation.
BC024883 mRNA Translation: AAH24883.1 Different initiation.
BC025560 mRNA Translation: AAH25560.1
CCDSiCCDS25582.1 [Q8R3E3-1]
RefSeqiNP_666052.1, NM_145940.2 [Q8R3E3-1]
UniGeneiMm.35817

3D structure databases

ProteinModelPortaliQ8R3E3
SMRiQ8R3E3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099349

PTM databases

iPTMnetiQ8R3E3
PhosphoSitePlusiQ8R3E3

Proteomic databases

PaxDbiQ8R3E3
PeptideAtlasiQ8R3E3
PRIDEiQ8R3E3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047186; ENSMUSP00000038635; ENSMUSG00000041895 [Q8R3E3-2]
ENSMUST00000103060; ENSMUSP00000099349; ENSMUSG00000041895 [Q8R3E3-1]
GeneIDi52639
KEGGimmu:52639
UCSCiuc007mcq.1 mouse [Q8R3E3-1]
uc007mcr.1 mouse [Q8R3E3-2]

Organism-specific databases

CTDi55062
MGIiMGI:1261864 Wipi1

Phylogenomic databases

eggNOGiKOG2110 Eukaryota
ENOG410XP6Y LUCA
GeneTreeiENSGT00730000110845
HOGENOMiHOG000217544
HOVERGENiHBG056639
InParanoidiQ8R3E3
KOiK17908
OMAiKMFMAAS
OrthoDBiEOG091G08ZS
PhylomeDBiQ8R3E3
TreeFamiTF314879

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy

Miscellaneous databases

ChiTaRSiWipi1 mouse
PROiPR:Q8R3E3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041895 Expressed in 278 organ(s), highest expression level in membranous labyrinth
CleanExiMM_WIPI1
ExpressionAtlasiQ8R3E3 baseline and differential
GenevisibleiQ8R3E3 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR036322 WD40_repeat_dom_sf
IPR032909 WIPI1
PANTHERiPTHR11227:SF23 PTHR11227:SF23, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 3 hits
SUPFAMiSSF50978 SSF50978, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWIPI1_MOUSE
AccessioniPrimary (citable) accession number: Q8R3E3
Secondary accession number(s): Q8BGE1, Q8R1A9, Q8R1C7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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