UniProtKB - Q8R3E3 (WIPI1_MOUSE)
Protein
WD repeat domain phosphoinositide-interacting protein 1
Gene
Wipi1
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy (By similarity) (PubMed:22275429). Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway (By similarity).By similarity1 Publication
GO - Molecular functioni
- androgen receptor binding Source: MGI
- estrogen receptor binding Source: MGI
- phosphatidylinositol-3,5-bisphosphate binding Source: UniProtKB
- phosphatidylinositol-3-phosphate binding Source: UniProtKB
- signaling receptor binding Source: MGI
GO - Biological processi
- autophagosome assembly Source: UniProtKB
- autophagy of mitochondrion Source: GO_Central
- autophagy of nucleus Source: GO_Central
- cellular response to starvation Source: UniProtKB
- protein lipidation Source: GO_Central
- protein localization to phagophore assembly site Source: GO_Central
- vesicle targeting, trans-Golgi to endosome Source: MGI
Keywordsi
Biological process | Autophagy |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy |
Names & Taxonomyi
Protein namesi | Recommended name: WD repeat domain phosphoinositide-interacting protein 1Short name: WIPI-1 Alternative name(s): WD40 repeat protein interacting with phosphoinositides of 49 kDa Short name: WIPI 49 kDa |
Gene namesi | Name:Wipi1 Synonyms:D11Ertd498e |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1261864, Wipi1 |
Subcellular locationi
Golgi apparatus
- trans-Golgi network By similarity
Cytoskeleton
- cytoskeleton By similarity
Endosome
- Endosome By similarity
Other locations
- clathrin-coated vesicle By similarity
- Preautophagosomal structure membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Trans elements of the Golgi and peripheral endosomes. Dynamically cycles through these compartments and is susceptible to conditions that modulate membrane flux. Enriched in clathrin-coated vesicles. Upon starvation-induced autophagy, accumulates at subcellular structures in the cytoplasm: enlarged vesicular and lasso-like structures, and large cup-shaped structures predominantly around the nucleus. Recruitment to autophagic membranes is controlled by MTMR14. Labile microtubules specifically recruit markers of autophagosome formation like WIPI1, whereas mature autophagosomes may bind to stable microtubules.By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: GO_Central
Endosome
- endosome membrane Source: MGI
Golgi apparatus
- trans-Golgi network Source: MGI
Other locations
- autophagosome membrane Source: MGI
- clathrin-coated vesicle Source: UniProtKB-SubCell
- cytoplasm Source: MGI
- extrinsic component of membrane Source: GO_Central
- phagophore assembly site Source: MGI
- phagophore assembly site membrane Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051438 | 1 – 446 | WD repeat domain phosphoinositide-interacting protein 1Add BLAST | 446 |
Proteomic databases
PaxDbi | Q8R3E3 |
PeptideAtlasi | Q8R3E3 |
PRIDEi | Q8R3E3 |
ProteomicsDBi | 299787 [Q8R3E3-1] 299788 [Q8R3E3-2] 299789 [Q8R3E3-3] |
PTM databases
iPTMneti | Q8R3E3 |
PhosphoSitePlusi | Q8R3E3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000041895, Expressed in membranous labyrinth and 294 other tissues |
ExpressionAtlasi | Q8R3E3, baseline and differential |
Genevisiblei | Q8R3E3, MM |
Interactioni
Subunit structurei
Interacts with androgen receptor (AR) and the estrogen receptors ESR1 and ESR2.
Interacts with WIPI2.
Interacts with WDR45.
Interacts with ATG16L1. May interact with NUDC.
By similarityGO - Molecular functioni
- androgen receptor binding Source: MGI
- estrogen receptor binding Source: MGI
- signaling receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 206708, 3 interactors |
STRINGi | 10090.ENSMUSP00000099349 |
Miscellaneous databases
RNActi | Q8R3E3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 184 – 224 | WD 1Add BLAST | 41 | |
Repeati | 230 – 269 | WD 2Add BLAST | 40 | |
Repeati | 312 – 351 | WD 3Add BLAST | 40 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 131 – 136 | Nuclear receptor interactionBy similarity | 6 |
Domaini
The N-terminus might form a beta-propeller domain involved in specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2), leading to the association of the protein to the membrane. Association to the membrane can also occur through binding to phosphatidylinositol 3-monophosphate (PI3P).
Sequence similaritiesi
Belongs to the WD repeat SVP1 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG2110, Eukaryota |
GeneTreei | ENSGT00940000156833 |
HOGENOMi | CLU_025895_1_0_1 |
InParanoidi | Q8R3E3 |
OMAi | CIHEGVE |
OrthoDBi | 1216824at2759 |
PhylomeDBi | Q8R3E3 |
TreeFami | TF314879 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf IPR032909, WIPI1 |
PANTHERi | PTHR11227:SF23, PTHR11227:SF23, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 3 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8R3E3-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEAEAADAPP GRVEAALSCF SFNQDCTSLA IGTKAGYKLF SLSSVEQLDQ
60 70 80 90 100
VHGSNEIPDV YIVERLFSSS LVVVVSHTKP RQMNVYHFKK GTEICNYSYS
110 120 130 140 150
SNILSIRLNR QRLLVCLEES IYIHNIKDMK LLKTVLDIPS NPTGLCALSI
160 170 180 190 200
NHSNSYLAYP GSQSTGEIVL YDGNSLKTVC TIAAHEGTLA AITFNSSGSK
210 220 230 240 250
LASASEKGTV IRVFSVPEGQ KLYEFRRGMK RYVTISSLVF SMDSQFLCAS
260 270 280 290 300
SNTETVHIFK MEHLTDSRPE EPSTWSGYMG KMFMAATNYL PAQVSDMMNQ
310 320 330 340 350
DRAFATGRLN FSGQKNICTL STIQKLPRLL VASSDGHLYI YNLDPQDGGE
360 370 380 390 400
CVLIKTHSLL SSGTTEENKE NDLRPSLPPS YAATVARPST SAASTVPGYS
410 420 430 440
EDGGALRGEV IPEHEFATGP VCLDDENEFP PIILCRGSQK GKTKQS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB1AT64 | B1AT64_MOUSE | WD repeat domain phosphoinositide-i... | Wipi1 | 295 | Annotation score: |
Sequence cautioni
The sequence AAH24811 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAH24883 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016967 | 267 – 270 | SRPE → RCRT in isoform 3. 1 Publication | 4 | |
Alternative sequenceiVSP_016968 | 271 – 446 | Missing in isoform 3. 1 PublicationAdd BLAST | 176 | |
Alternative sequenceiVSP_016969 | 432 – 446 | IILCR…KTKQS → VSIRNP in isoform 2. 2 PublicationsAdd BLAST | 15 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031672 mRNA Translation: BAC27504.1 AK034907 mRNA Translation: BAC28878.1 AK050495 mRNA Translation: BAE20673.1 AK159608 mRNA Translation: BAE35227.1 AK170563 mRNA Translation: BAE41884.1 BC024811 mRNA Translation: AAH24811.1 Different initiation. BC024883 mRNA Translation: AAH24883.1 Different initiation. BC025560 mRNA Translation: AAH25560.1 |
CCDSi | CCDS25582.1 [Q8R3E3-1] |
RefSeqi | NP_666052.1, NM_145940.2 [Q8R3E3-1] |
Genome annotation databases
Ensembli | ENSMUST00000047186; ENSMUSP00000038635; ENSMUSG00000041895 [Q8R3E3-2] ENSMUST00000103060; ENSMUSP00000099349; ENSMUSG00000041895 [Q8R3E3-1] |
GeneIDi | 52639 |
KEGGi | mmu:52639 |
UCSCi | uc007mcq.1, mouse [Q8R3E3-1] uc007mcr.1, mouse [Q8R3E3-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031672 mRNA Translation: BAC27504.1 AK034907 mRNA Translation: BAC28878.1 AK050495 mRNA Translation: BAE20673.1 AK159608 mRNA Translation: BAE35227.1 AK170563 mRNA Translation: BAE41884.1 BC024811 mRNA Translation: AAH24811.1 Different initiation. BC024883 mRNA Translation: AAH24883.1 Different initiation. BC025560 mRNA Translation: AAH25560.1 |
CCDSi | CCDS25582.1 [Q8R3E3-1] |
RefSeqi | NP_666052.1, NM_145940.2 [Q8R3E3-1] |
3D structure databases
SMRi | Q8R3E3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206708, 3 interactors |
STRINGi | 10090.ENSMUSP00000099349 |
PTM databases
iPTMneti | Q8R3E3 |
PhosphoSitePlusi | Q8R3E3 |
Proteomic databases
PaxDbi | Q8R3E3 |
PeptideAtlasi | Q8R3E3 |
PRIDEi | Q8R3E3 |
ProteomicsDBi | 299787 [Q8R3E3-1] 299788 [Q8R3E3-2] 299789 [Q8R3E3-3] |
Protocols and materials databases
Antibodypediai | 1968, 278 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000047186; ENSMUSP00000038635; ENSMUSG00000041895 [Q8R3E3-2] ENSMUST00000103060; ENSMUSP00000099349; ENSMUSG00000041895 [Q8R3E3-1] |
GeneIDi | 52639 |
KEGGi | mmu:52639 |
UCSCi | uc007mcq.1, mouse [Q8R3E3-1] uc007mcr.1, mouse [Q8R3E3-2] |
Organism-specific databases
CTDi | 55062 |
MGIi | MGI:1261864, Wipi1 |
Phylogenomic databases
eggNOGi | KOG2110, Eukaryota |
GeneTreei | ENSGT00940000156833 |
HOGENOMi | CLU_025895_1_0_1 |
InParanoidi | Q8R3E3 |
OMAi | CIHEGVE |
OrthoDBi | 1216824at2759 |
PhylomeDBi | Q8R3E3 |
TreeFami | TF314879 |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy |
Miscellaneous databases
BioGRID-ORCSi | 52639, 1 hit in 53 CRISPR screens |
ChiTaRSi | Wipi1, mouse |
PROi | PR:Q8R3E3 |
RNActi | Q8R3E3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041895, Expressed in membranous labyrinth and 294 other tissues |
ExpressionAtlasi | Q8R3E3, baseline and differential |
Genevisiblei | Q8R3E3, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf IPR032909, WIPI1 |
PANTHERi | PTHR11227:SF23, PTHR11227:SF23, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 3 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | WIPI1_MOUSE | |
Accessioni | Q8R3E3Primary (citable) accession number: Q8R3E3 Secondary accession number(s): Q8BGE1, Q8R1A9, Q8R1C7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2006 |
Last sequence update: | June 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families