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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

Gene

Plcd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. Two of the Ca2+ ions are bound to the C2 domain.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei311PROSITE-ProRule annotation1
Metal bindingi312Calcium 1; catalyticBy similarity1
Metal bindingi341Calcium 1; catalyticBy similarity1
Metal bindingi343Calcium 1; catalyticBy similarity1
Active sitei356PROSITE-ProRule annotation1
Metal bindingi390Calcium 1; catalyticBy similarity1
Binding sitei438SubstrateBy similarity1
Binding sitei440SubstrateBy similarity1
Binding sitei522SubstrateBy similarity1
Binding sitei549SubstrateBy similarity1
Metal bindingi651Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi653Calcium 2By similarity1
Metal bindingi677Calcium 2By similarity1
Metal bindingi706Calcium 3By similarity1
Metal bindingi707Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi708Calcium 3By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi153 – 1641PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi189 – 2002PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.4.11 3474
ReactomeiR-MMU-1855204 Synthesis of IP3 and IP4 in the cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-delta-1
Phospholipase C-delta-1
Short name:
PLC-delta-1
Gene namesi
Name:Plcd1
Synonyms:Plcd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:97614 Plcd1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Mice lacking Plcd1 and Plcd3 die between 11.5 and 13.5 dpc. They exhibit severe disruption of the normal labyrinth architecture in the placenta and decreased placental vascularization, as well as abnormal proliferation and apoptosis of trophoblasts in the labyrinth area. Furthermore, Plcd1 and Plcd3 double knockout embryos supplied with a normal placenta by the tetraploid aggregation method survive beyond 14.5 dpc, indicating that the embryonic lethality is caused by a defect in trophoblasts.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000885051 – 7561-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1Add BLAST756

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi191O-linked (GlcNAc) serineBy similarity1
Glycosylationi193O-linked (GlcNAc) threonineBy similarity1
Modified residuei457PhosphothreonineCombined sources1
Modified residuei460PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8R3B1
MaxQBiQ8R3B1
PaxDbiQ8R3B1
PeptideAtlasiQ8R3B1
PRIDEiQ8R3B1

PTM databases

iPTMnetiQ8R3B1
PhosphoSitePlusiQ8R3B1

Expressioni

Tissue specificityi

Highly expressed in brain, heart, lung, epididymis and testis. Detected at lower levels in kidney and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSMUSG00000010660
CleanExiMM_PLCD1
ExpressionAtlasiQ8R3B1 baseline and differential
GenevisibleiQ8R3B1 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010804

Structurei

3D structure databases

ProteinModelPortaliQ8R3B1
SMRiQ8R3B1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 130PHPROSITE-ProRule annotationAdd BLAST110
Domaini140 – 175EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini176 – 211EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini296 – 440PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST145
Domaini492 – 609PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST118
Domaini630 – 720C2PROSITE-ProRule annotationAdd BLAST91

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 57Substrate bindingBy similarityAdd BLAST28

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00760000118936
HOGENOMiHOG000006871
HOVERGENiHBG053610
InParanoidiQ8R3B1
KOiK05857
OMAiCKTIWQE
OrthoDBiEOG091G02KH
TreeFamiTF313216

Family and domain databases

Gene3Di2.30.29.30, 1 hit
2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001192 PI-PLC_fam
IPR028391 PLC-delta1
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF80 PTHR10336:SF80, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF16457 PH_12, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00233 PH, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS50003 PH_DOMAIN, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R3B1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSGRDFLTL HGLQDDPDLQ ALLKGSQLLK VKSSSWRRER FYKLQEDCKT
60 70 80 90 100
IWQESRKVMR SPESQLFSIE DIQEVRMGHR TEGLEKFARD IPEDRCFSIV
110 120 130 140 150
FKDQRNTLDL IAPSPADVQH WVQGLRKIID RSGSMDQRQK LQHWIHSCLR
160 170 180 190 200
KADKNKDNKM NFKEVKDFLK ELNVQVDDSY ARKIFRECDH SQTDSLEDEE
210 220 230 240 250
IETFYRMLTQ RAEIDRAFAE AAGSAETLSV EKLVTFLQHQ QREEEAGPAL
260 270 280 290 300
ALSLIERYEP SETAKAQRQM TKDGFLMYLL SADGNAFSLA HRRVYQDMNQ
310 320 330 340 350
PLSHYLVSSS HNTYLLEDQL TGPSSTEAYI RALCKGCRCL ELDCWDGPNQ
360 370 380 390 400
EPIIYHGYTF TSKILFCDVL RAIRDYAFKA SPYPVILSLE NHCSLEQQRV
410 420 430 440 450
MAHHLRAILG PMLLDQPLDG VTTSLPSPEQ LKEKILLKGK KLGGLLPAGG
460 470 480 490 500
ENGPEATDVS DEDEAAEMED EAVRSQVQHK PKEDKLKLVP ELSDMVIYCK
510 520 530 540 550
SVHFGGFSSP STSGQAFYEM ASFSESRALR LLQESGNSFV RHNVGHLSRI
560 570 580 590 600
YPAGWRTDSS NYSPVEMWNG GCQIVALNFQ TPGPEMDVYL GCFQDNGGCG
610 620 630 640 650
YVLKPAFLRD PDTTFNSRAL TQGPWWAPKK LRVWIISGQQ LPKVNKNKNS
660 670 680 690 700
IVDPKVIVEI HGVGQDVASR QTAVITNNGF NPRWDTEFEF VVAVPDLALV
710 720 730 740 750
RFMVEDYDSS SKNDFIGQST IPWNSLKQGY RHVHLLSKNG DLHPSATLFV

KISIQD
Length:756
Mass (Da):85,873
Last modified:July 27, 2011 - v2
Checksum:i71F17810F9B0B938
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti212A → V in AAH25798 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF133125 mRNA Translation: AAD32616.1
U85711 mRNA Translation: AAD00570.1
AK028749 mRNA Translation: BAC26096.1
AK082890 mRNA Translation: BAC38671.1
CH466587 Genomic DNA Translation: EDL09242.1
BC025798 mRNA Translation: AAH25798.1
CCDSiCCDS23607.1
RefSeqiNP_001280577.1, NM_001293648.1
NP_062650.1, NM_019676.3
UniGeneiMm.23963

Genome annotation databases

EnsembliENSMUST00000010804; ENSMUSP00000010804; ENSMUSG00000010660
GeneIDi18799
KEGGimmu:18799
UCSCiuc009saf.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPLCD1_MOUSE
AccessioniPrimary (citable) accession number: Q8R3B1
Secondary accession number(s): Q9Z1B4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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