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Protein

Cell division cycle protein 16 homolog

Gene

Cdc16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-MMU-176408 Regulation of APC/C activators between G1/S and early anaphase
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-176412 Phosphorylation of the APC/C
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein 16 homolog
Alternative name(s):
Anaphase-promoting complex subunit 6
Short name:
APC6
Cyclosome subunit 6
Gene namesi
Name:Cdc16
Synonyms:Anapc6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1917207 Cdc16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001062681 – 620Cell division cycle protein 16 homologAdd BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei490PhosphoserineBy similarity1
Modified residuei560PhosphoserineCombined sources1
Modified residuei599PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8R349
MaxQBiQ8R349
PaxDbiQ8R349
PRIDEiQ8R349

PTM databases

iPTMnetiQ8R349
PhosphoSitePlusiQ8R349

Expressioni

Gene expression databases

BgeeiENSMUSG00000038416 Expressed in 294 organ(s), highest expression level in midbrain
ExpressionAtlasiQ8R349 baseline and differential
GenevisibleiQ8R349 MM

Interactioni

Subunit structurei

V-shaped homodimer. The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5. Interacts with PPP5C and CDC20. Interacts with CDC26.By similarity

Protein-protein interaction databases

BioGridi213776, 47 interactors
CORUMiQ8R349
IntActiQ8R349, 50 interactors
MINTiQ8R349
STRINGi10090.ENSMUSP00000047950

Structurei

3D structure databases

ProteinModelPortaliQ8R349
SMRiQ8R349
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 33TPR 1Add BLAST30
Repeati37 – 62TPR 2Add BLAST26
Repeati70 – 93TPR 3Add BLAST24
Repeati128 – 159TPR 4Add BLAST32
Repeati164 – 187TPR 5Add BLAST24
Repeati198 – 222TPR 6Add BLAST25
Repeati232 – 260TPR 7Add BLAST29
Repeati267 – 294TPR 8Add BLAST28
Repeati299 – 329TPR 9Add BLAST31
Repeati334 – 362TPR 10Add BLAST29
Repeati369 – 397TPR 11Add BLAST29
Repeati402 – 434TPR 12Add BLAST33
Repeati442 – 474TPR 13Add BLAST33
Repeati479 – 508TPR 14Add BLAST30

Domaini

TPR repeats 1-7 mediate homodimerization, while the C-terminal TPR repeats bind to CDC26, burying its hydrophobic N-terminus.By similarity

Sequence similaritiesi

Belongs to the APC6/CDC16 family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1173 Eukaryota
ENOG410XNY1 LUCA
GeneTreeiENSGT00510000047327
HOGENOMiHOG000230908
HOVERGENiHBG050857
InParanoidiQ8R349
KOiK03353
OMAiYIDQQQY
OrthoDBiEOG091G0HW1
PhylomeDBiQ8R349
TreeFamiTF101054

Family and domain databases

InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
SMARTiView protein in SMART
SM00028 TPR, 6 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 5 hits
PS50293 TPR_REGION, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8R349-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNLEPLRKRV RQYLDQQQYQ SALFWADKVA SLSHEEPQDV YWLAQCLYLT
60 70 80 90 100
AQYHRAAHAL RSRKLDKLYE ACRYLAARCH YAAKEHQQAL DILDMEEPIN
110 120 130 140 150
RRLFEKYLKD DNGSRDPSSD WEMSQSSIKS SICLLRGKIY DALDNRTLAT
160 170 180 190 200
YSYKEALKLD VYCFEAFDLL TSHHMLTAQE EKELLDSLPL NKLCAEEQEL
210 220 230 240 250
LRFVFENKLK KYNKPSETVI PESVDGLQEN LDVVVSLAER HYYNCDFKMC
260 270 280 290 300
YKLTSTVMEK DPFHANCLPV HIGTLVELNK ANELFYLSHK LVDLYPSNPV
310 320 330 340 350
SWFAVGCYYL MVGHKNEHAR RYLSKATTLE KTYGPAWIAY GHSFAVESEH
360 370 380 390 400
DQAMAAYFTA AQLMKGCHLP MLYIGLEYGL TNNSKLAERF FGQALSIAPE
410 420 430 440 450
DPFVIHEVGV VAFQNGEWKT AEKWFLDALE KIKAIGNEVT VDKWEPLLNN
460 470 480 490 500
LGHVCRKLKK YAEALDYHRQ ALVLIPQNAS TYSAIGYIHS LMGNFENAVD
510 520 530 540 550
YFHTALGLRR DDTFSVTMLG HCIEMYIGDS EAYIGADIKD KLKCYDFDVH
560 570 580 590 600
TMKTLKNIIS PPWDFRDFEV EKQNTEEAGL APLQNSTKAP ESRPNLEETF
610 620
EIEMNESDMM LETSMSDHST
Length:620
Mass (Da):71,460
Last modified:June 1, 2002 - v1
Checksum:iA0E47748506CC14E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TZ92Q3TZ92_MOUSE
Cell division cycle protein 16 homo...
Cdc16
85Annotation score:
A0A1B0GR68A0A1B0GR68_MOUSE
Cell division cycle protein 16 homo...
Cdc16
188Annotation score:

Sequence cautioni

The sequence BAB28717 differs from that shown. Intron retention.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026606 mRNA Translation: AAH26606.1
AK013213 mRNA Translation: BAB28717.1 Sequence problems.
CCDSiCCDS22114.1
RefSeqiNP_081552.2, NM_027276.2
UniGeneiMm.182412

Genome annotation databases

EnsembliENSMUST00000043962; ENSMUSP00000047950; ENSMUSG00000038416
GeneIDi69957
KEGGimmu:69957
UCSCiuc009kyi.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026606 mRNA Translation: AAH26606.1
AK013213 mRNA Translation: BAB28717.1 Sequence problems.
CCDSiCCDS22114.1
RefSeqiNP_081552.2, NM_027276.2
UniGeneiMm.182412

3D structure databases

ProteinModelPortaliQ8R349
SMRiQ8R349
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213776, 47 interactors
CORUMiQ8R349
IntActiQ8R349, 50 interactors
MINTiQ8R349
STRINGi10090.ENSMUSP00000047950

PTM databases

iPTMnetiQ8R349
PhosphoSitePlusiQ8R349

Proteomic databases

EPDiQ8R349
MaxQBiQ8R349
PaxDbiQ8R349
PRIDEiQ8R349

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043962; ENSMUSP00000047950; ENSMUSG00000038416
GeneIDi69957
KEGGimmu:69957
UCSCiuc009kyi.1 mouse

Organism-specific databases

CTDi8881
MGIiMGI:1917207 Cdc16

Phylogenomic databases

eggNOGiKOG1173 Eukaryota
ENOG410XNY1 LUCA
GeneTreeiENSGT00510000047327
HOGENOMiHOG000230908
HOVERGENiHBG050857
InParanoidiQ8R349
KOiK03353
OMAiYIDQQQY
OrthoDBiEOG091G0HW1
PhylomeDBiQ8R349
TreeFamiTF101054

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-MMU-176408 Regulation of APC/C activators between G1/S and early anaphase
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-176412 Phosphorylation of the APC/C
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiCdc16 mouse
PROiPR:Q8R349
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038416 Expressed in 294 organ(s), highest expression level in midbrain
ExpressionAtlasiQ8R349 baseline and differential
GenevisibleiQ8R349 MM

Family and domain databases

InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
SMARTiView protein in SMART
SM00028 TPR, 6 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 5 hits
PS50293 TPR_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCDC16_MOUSE
AccessioniPrimary (citable) accession number: Q8R349
Secondary accession number(s): Q9CYX9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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