UniProtKB - Q8R307 (VPS18_MOUSE)
Vacuolar protein sorting-associated protein 18 homolog
Vps18
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 853 – 947 | RING-typeAdd BLAST | 95 |
GO - Molecular functioni
- actin binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- protein-macromolecule adaptor activity Source: GO_Central
- syntaxin binding Source: MGI
GO - Biological processi
- autophagy Source: UniProtKB-KW
- endosome organization Source: MGI
- endosome to lysosome transport Source: MGI
- intracellular protein transport Source: InterPro
- lysosome organization Source: MGI
- regulation of SNARE complex assembly Source: GO_Central
- regulation of synaptic vesicle exocytosis Source: MGI
- vacuole organization Source: GO_Central
- vesicle docking involved in exocytosis Source: GO_Central
- viral entry into host cell Source: MGI
Keywordsi
Biological process | Autophagy, Protein transport, Transport |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 18 homolog |
Gene namesi | Name:Vps18 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2443626, Vps18 |
Subcellular locationi
Lysosome
- Lysosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Endosome
- Late endosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
- Early endosome By similarity
Other locations
- autophagosome Curated
- clathrin-coated vesicle Curated
Note: Cytoplasmic, peripheral membrane protein associated with early endosomes and late endosomes/lysosomes.
Cytoskeleton
- actin filament Source: MGI
Endosome
- CORVET complex Source: UniProtKB
- early endosome Source: MGI
- endosome Source: GO_Central
- late endosome Source: MGI
- late endosome membrane Source: UniProtKB-SubCell
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
- lysosome Source: MGI
Other locations
- autophagosome Source: UniProtKB-SubCell
- clathrin-coated vesicle Source: UniProtKB-SubCell
- glutamatergic synapse Source: MGI
- HOPS complex Source: MGI
- presynapse Source: Ensembl
Keywords - Cellular componenti
Cytoplasmic vesicle, Endosome, Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000055907 | 2 – 973 | Vacuolar protein sorting-associated protein 18 homologAdd BLAST | 972 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 3 | PhosphoserineBy similarity | 1 | |
Modified residuei | 11 | PhosphoserineBy similarity | 1 | |
Modified residuei | 13 | PhosphoserineBy similarity | 1 | |
Modified residuei | 362 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 689 | PhosphoserineBy similarity | 1 | |
Modified residuei | 912 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8R307 |
MaxQBi | Q8R307 |
PaxDbi | Q8R307 |
PeptideAtlasi | Q8R307 |
PRIDEi | Q8R307 |
PTM databases
iPTMneti | Q8R307 |
PhosphoSitePlusi | Q8R307 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000034216, Expressed in muscle tissue and 234 other tissues |
Genevisiblei | Q8R307, MM |
Interactioni
Subunit structurei
Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1.
Interacts with RAB5C (PubMed:25266290).
Interacts with HOOK1 (PubMed:14668490).
Interacts with STX7, MON1B (By similarity). Associates with adaptor protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634).
Interacts with SYNPO2 (By similarity).
By similarity3 PublicationsBinary interactionsi
Q8R307
With | #Exp. | IntAct |
---|---|---|
VIPAS39 [Q9H9C1] from Homo sapiens. | 5 | EBI-2527788,EBI-749080 |
GO - Molecular functioni
- actin binding Source: MGI
- syntaxin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 230740, 18 interactors |
IntActi | Q8R307, 4 interactors |
STRINGi | 10090.ENSMUSP00000036915 |
Miscellaneous databases
RNActi | Q8R307, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 618 – 772 | CHCRAdd BLAST | 155 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 454 – 481 | Sequence analysisAdd BLAST | 28 | |
Coiled coili | 802 – 848 | Sequence analysisAdd BLAST | 47 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 853 – 947 | RING-typeAdd BLAST | 95 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2034, Eukaryota |
GeneTreei | ENSGT00940000153635 |
HOGENOMi | CLU_003488_1_0_1 |
InParanoidi | Q8R307 |
OMAi | EHCFQNK |
OrthoDBi | 87842at2759 |
PhylomeDBi | Q8R307 |
TreeFami | TF105704 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR000547, Clathrin_H-chain/VPS_repeat IPR007810, Pep3_Vps18 IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF00637, Clathrin, 1 hit PF05131, Pep3_Vps18, 1 hit |
PROSITEi | View protein in PROSITE PS50236, CHCR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MASILDEYED SLSRSAVLQT GCPSVGIPHS GYVSAHLEKE VPIFTKQRVD
60 70 80 90 100
FTPSERITSL VVSCNQLCMS LGKDTLLRID LGKASEPNRV ELGRKDDAKV
110 120 130 140 150
HKMFLDHTGS HLLVALSSTE VLYMNRNGQK ARPLARWKGQ LVESVGWNKA
160 170 180 190 200
MGNESSTGPI LVGTAQGQIF EAELSASEGG LFGPAPDLYF RPLYVLNEEG
210 220 230 240 250
GPAPVCSLEA ERGPDGRGFV IATTRQRLFQ FIGRAVEDTE AQGFAGLFAA
260 270 280 290 300
YTDHPPPFRE FPSNLGYSEL AFYTPKLRSA PRAFAWMMGD GVLYGSLDCG
310 320 330 340 350
RPDSLLSEER VWEYPAGVGP GANPPLAIVL TQFHFLLLLA DRVEAVCTLT
360 370 380 390 400
GQVVLRDHFL EKFGPLRHMV KDSSTGHLWA YTERAVFRYH VQREARDVWR
410 420 430 440 450
TYLDMNRFDL AKEYCRERPD CLDTVLAREA DFCFRQHRYL ESARCYALTQ
460 470 480 490 500
SYFEEIALKF LEARQEEALA EFLQRKLAGL KPTERTQATL LTTWLTELYL
510 520 530 540 550
SRLGALQGDP DALTLYRDTR ECFRTFLSSP RHKEWLFASR ASIHELLASH
560 570 580 590 600
GDTEHMVYFA VIMQDYERVV AYHCQHEAYE EALAVLARHR DPQLFYKFSP
610 620 630 640 650
ILIRHIPRQL VDAWIEMGSR LDARQLIPAL VNYSQGGEAQ QVSQAIRYME
660 670 680 690 700
FCVNVLGETE QAIHNYLLSL YARGQPASLL AYLEQAGASP HRVHYDLKYA
710 720 730 740 750
LRLCAEHGHH RACVHVYKVL ELYEEAVDLA LQVDVDLAKQ CADLPEEDEE
760 770 780 790 800
LRKKLWLKIA RHVVQEEEDV QTAMACLASC PLLKIEDVLP FFPDFVTIDH
810 820 830 840 850
FKEAICSSLK AYNHHIQELQ REMEEATASA QRIRRDLQEL RGRYGTVEPQ
860 870 880 890 900
DKCSTCDFPL LNRPFYLFLC GHMFHADCLL QAVRPGLPAY KQARLEELQR
910 920 930 940 950
KLGAAPPPTK GSVKAKEAEA GAAAVGPSRE QLKADLDELV AAECVYCGEL
960 970
MIRSIDRPFI DPQRYEEEHL SWL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 676 | P → T in BAC28236 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 943 | E → G in BAC26302 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK029109 mRNA Translation: BAC26302.1 AK033333 mRNA Translation: BAC28236.1 AK036915 mRNA Translation: BAC29637.1 BC026870 mRNA Translation: AAH26870.1 BC036129 mRNA Translation: AAH36129.1 BC039176 mRNA Translation: AAH39176.1 |
CCDSi | CCDS16599.1 |
RefSeqi | NP_758473.3, NM_172269.3 |
Genome annotation databases
Ensembli | ENSMUST00000037280; ENSMUSP00000036915; ENSMUSG00000034216 |
GeneIDi | 228545 |
KEGGi | mmu:228545 |
UCSCi | uc008lto.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK029109 mRNA Translation: BAC26302.1 AK033333 mRNA Translation: BAC28236.1 AK036915 mRNA Translation: BAC29637.1 BC026870 mRNA Translation: AAH26870.1 BC036129 mRNA Translation: AAH36129.1 BC039176 mRNA Translation: AAH39176.1 |
CCDSi | CCDS16599.1 |
RefSeqi | NP_758473.3, NM_172269.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 230740, 18 interactors |
IntActi | Q8R307, 4 interactors |
STRINGi | 10090.ENSMUSP00000036915 |
PTM databases
iPTMneti | Q8R307 |
PhosphoSitePlusi | Q8R307 |
Proteomic databases
EPDi | Q8R307 |
MaxQBi | Q8R307 |
PaxDbi | Q8R307 |
PeptideAtlasi | Q8R307 |
PRIDEi | Q8R307 |
Protocols and materials databases
Antibodypediai | 23197, 142 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000037280; ENSMUSP00000036915; ENSMUSG00000034216 |
GeneIDi | 228545 |
KEGGi | mmu:228545 |
UCSCi | uc008lto.2, mouse |
Organism-specific databases
CTDi | 57617 |
MGIi | MGI:2443626, Vps18 |
Phylogenomic databases
eggNOGi | KOG2034, Eukaryota |
GeneTreei | ENSGT00940000153635 |
HOGENOMi | CLU_003488_1_0_1 |
InParanoidi | Q8R307 |
OMAi | EHCFQNK |
OrthoDBi | 87842at2759 |
PhylomeDBi | Q8R307 |
TreeFami | TF105704 |
Miscellaneous databases
BioGRID-ORCSi | 228545, 7 hits in 17 CRISPR screens |
ChiTaRSi | Vps18, mouse |
PROi | PR:Q8R307 |
RNActi | Q8R307, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034216, Expressed in muscle tissue and 234 other tissues |
Genevisiblei | Q8R307, MM |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR000547, Clathrin_H-chain/VPS_repeat IPR007810, Pep3_Vps18 IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF00637, Clathrin, 1 hit PF05131, Pep3_Vps18, 1 hit |
PROSITEi | View protein in PROSITE PS50236, CHCR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS18_MOUSE | |
Accessioni | Q8R307Primary (citable) accession number: Q8R307 Secondary accession number(s): Q8BGV6, Q8BZX6, Q8C126 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2002 |
Last sequence update: | June 7, 2004 | |
Last modified: | February 10, 2021 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families