Vps18

Functionⁱ

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (By similarity). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:14517315, PubMed:22854957). Involved in dendrite development of Pukinje cells (PubMed:22699122).By similarity3 Publications

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	853 – 947	RING-typeAdd BLAST		95

GO - Molecular functionⁱ

actin binding
Source: MGI
Inferred from direct assayⁱ
- 14668490
metal ion binding Source: UniProtKB-KW
protein binding, bridging
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
syntaxin binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

autophagy Source: UniProtKB-KW
endosome organization
Source: MGI
Inferred from direct assayⁱ
- 14668490
endosome to lysosome transport Source: MGI
intracellular protein transport Source: InterPro
lysosome organization Source: MGI
regulation of SNARE complex assembly
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of synaptic vesicle exocytosis Source: MGI
vesicle docking involved in exocytosis
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
viral entry into host cell
Source: MGI
Inferred from mutant phenotypeⁱ
- 24550300

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Autophagy, Protein transport, Transport
Ligand	Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Vacuolar protein sorting-associated protein 18 homolog
Gene namesⁱ	Name:Vps18
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 2

Organism-specific databases

MGIⁱ	MGI:2443626 Vps18

Keywords - Cellular componentⁱ

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000055907	2 – 973	Vacuolar protein sorting-associated protein 18 homologAdd BLAST		972

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylalanineBy similarity	1
Modified residueⁱ	3	PhosphoserineBy similarity	1
Modified residueⁱ	11	PhosphoserineBy similarity	1
Modified residueⁱ	13	PhosphoserineBy similarity	1
Modified residueⁱ	362	N6-acetyllysineBy similarity	1
Modified residueⁱ	689	PhosphoserineBy similarity	1
Modified residueⁱ	912	PhosphoserineBy similarity	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	Q8R307
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q8R307
PaxDbⁱ	Q8R307
PeptideAtlas More...PeptideAtlasⁱ	Q8R307
PRIDEⁱ	Q8R307

PTM databases

iPTMnetⁱ	Q8R307
PhosphoSitePlusⁱ	Q8R307

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000034216 Expressed in 212 organ(s), highest expression level in pancreas
CleanExⁱ	MM_VPS18
Genevisibleⁱ	Q8R307 MM

Interactionⁱ

Subunit structureⁱ

Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1. Interacts with RAB5C (PubMed:25266290). Interacts with HOOK1 (PubMed:14668490). Interacts with STX7, MON1B (By similarity). Associates with adaptor protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634). Interacts with SYNPO2 (By similarity).By similarity3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
VIPAS39	Q9H9C1	5	EBI-2527788,EBI-749080	From Homo sapiens.

With

Entry

#Exp.

IntAct

Notes

VIPAS39

Q9H9C1

5

EBI-2527788,EBI-749080

From Homo sapiens.

GO - Molecular functionⁱ

actin binding
Source: MGI
Inferred from direct assayⁱ
- 14668490
protein binding, bridging
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
syntaxin binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	230740, 17 interactors
IntActⁱ	Q8R307, 4 interactors
STRINGⁱ	10090.ENSMUSP00000036915

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8R307
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Repeatⁱ	618 – 772	CHCRAdd BLAST		155

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	454 – 481	Sequence analysisAdd BLAST		28
Coiled coilⁱ	802 – 848	Sequence analysisAdd BLAST		47

Sequence similaritiesⁱ

Belongs to the VPS18 family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	853 – 947	RING-typeAdd BLAST		95

Keywords - Domainⁱ

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG2034 Eukaryota ENOG410XRSD LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000015233
HOVERGENⁱ	HBG057876
InParanoidⁱ	Q8R307
KEGG Orthology (KO) More...KOⁱ	K20181
OMAⁱ	DAWIEMG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G01XI
PhylomeDBⁱ	Q8R307
TreeFamⁱ	TF105704

Family and domain databases

Gene3Dⁱ	1.25.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR000547 Clathrin_H-chain/VPS_repeat IPR007810 Pep3_Vps18 IPR011990 TPR-like_helical_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00637 Clathrin, 1 hit PF05131 Pep3_Vps18, 1 hit
PROSITEⁱ	View protein in PROSITE PS50236 CHCR, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q8R307-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MASILDEYED SLSRSAVLQT GCPSVGIPHS GYVSAHLEKE VPIFTKQRVD 
        60         70         80         90        100
FTPSERITSL VVSCNQLCMS LGKDTLLRID LGKASEPNRV ELGRKDDAKV 
       110        120        130        140        150
HKMFLDHTGS HLLVALSSTE VLYMNRNGQK ARPLARWKGQ LVESVGWNKA 
       160        170        180        190        200
MGNESSTGPI LVGTAQGQIF EAELSASEGG LFGPAPDLYF RPLYVLNEEG 
       210        220        230        240        250
GPAPVCSLEA ERGPDGRGFV IATTRQRLFQ FIGRAVEDTE AQGFAGLFAA 
       260        270        280        290        300
YTDHPPPFRE FPSNLGYSEL AFYTPKLRSA PRAFAWMMGD GVLYGSLDCG 
       310        320        330        340        350
RPDSLLSEER VWEYPAGVGP GANPPLAIVL TQFHFLLLLA DRVEAVCTLT 
       360        370        380        390        400
GQVVLRDHFL EKFGPLRHMV KDSSTGHLWA YTERAVFRYH VQREARDVWR 
       410        420        430        440        450
TYLDMNRFDL AKEYCRERPD CLDTVLAREA DFCFRQHRYL ESARCYALTQ 
       460        470        480        490        500
SYFEEIALKF LEARQEEALA EFLQRKLAGL KPTERTQATL LTTWLTELYL 
       510        520        530        540        550
SRLGALQGDP DALTLYRDTR ECFRTFLSSP RHKEWLFASR ASIHELLASH 
       560        570        580        590        600
GDTEHMVYFA VIMQDYERVV AYHCQHEAYE EALAVLARHR DPQLFYKFSP 
       610        620        630        640        650
ILIRHIPRQL VDAWIEMGSR LDARQLIPAL VNYSQGGEAQ QVSQAIRYME 
       660        670        680        690        700
FCVNVLGETE QAIHNYLLSL YARGQPASLL AYLEQAGASP HRVHYDLKYA 
       710        720        730        740        750
LRLCAEHGHH RACVHVYKVL ELYEEAVDLA LQVDVDLAKQ CADLPEEDEE 
       760        770        780        790        800
LRKKLWLKIA RHVVQEEEDV QTAMACLASC PLLKIEDVLP FFPDFVTIDH 
       810        820        830        840        850
FKEAICSSLK AYNHHIQELQ REMEEATASA QRIRRDLQEL RGRYGTVEPQ 
       860        870        880        890        900
DKCSTCDFPL LNRPFYLFLC GHMFHADCLL QAVRPGLPAY KQARLEELQR 
       910        920        930        940        950
KLGAAPPPTK GSVKAKEAEA GAAAVGPSRE QLKADLDELV AAECVYCGEL 
       960        970 
MIRSIDRPFI DPQRYEEEHL SWL

Length:973

Mass (Da):110,219

Last modified:June 7, 2004 - v2

Checksum:ⁱ6B4E90FC39DC897F

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	676	P → T in BAC28236 (PubMed:16141072).Curated		1
Sequence conflictⁱ	943	E → G in BAC26302 (PubMed:16141072).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK029109 mRNA Translation: BAC26302.1 AK033333 mRNA Translation: BAC28236.1 AK036915 mRNA Translation: BAC29637.1 BC026870 mRNA Translation: AAH26870.1 BC036129 mRNA Translation: AAH36129.1 BC039176 mRNA Translation: AAH39176.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS16599.1
NCBI Reference Sequences More...RefSeqⁱ	NP_758473.3, NM_172269.3
UniGeneⁱ	Mm.294761

Genome annotation databases

Ensemblⁱ	ENSMUST00000037280; ENSMUSP00000036915; ENSMUSG00000034216
GeneIDⁱ	228545
KEGGⁱ	mmu:228545
UCSC genome browser More...UCSCⁱ	uc008lto.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8R307	MKIAA1475 protein (Fragment)		MOUSE		787	UniRef100_Q8R307

Protein	Similar proteins		Species	Score	Length	Source
Q8R307	Vacuolar protein sorting-associated protein 18 homolog		HUMAN		973	UniRef90_Q9P253
Vacuolar protein sorting-associated protein 18 homolog		HETGA		974
Vacuolar protein sorting-associated protein 18 like protein		FUKDA		974
Uncharacterized protein		CAVPO		974
Uncharacterized protein		MOUSE		974
+149

Protein	Similar proteins		Species	Score	Length	Source
Q8R307	Vacuolar protein sorting-associated protein 18 homolog		HUMAN		973	UniRef50_Q9P253
Uncharacterized protein		CAVPO		974
Uncharacterized protein		MOUSE		974
Vacuolar protein sorting-associated protein 18 homolog		HETGA		974
Vacuolar protein sorting-associated protein 18 like protein		FUKDA		974
+350

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK029109 mRNA Translation: BAC26302.1 AK033333 mRNA Translation: BAC28236.1 AK036915 mRNA Translation: BAC29637.1 BC026870 mRNA Translation: AAH26870.1 BC036129 mRNA Translation: AAH36129.1 BC039176 mRNA Translation: AAH39176.1
CCDSⁱ	CCDS16599.1
RefSeqⁱ	NP_758473.3, NM_172269.3
UniGeneⁱ	Mm.294761

3D structure databases

ProteinModelPortalⁱ	Q8R307
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	230740, 17 interactors
IntActⁱ	Q8R307, 4 interactors
STRINGⁱ	10090.ENSMUSP00000036915

PTM databases

iPTMnetⁱ	Q8R307
PhosphoSitePlusⁱ	Q8R307

Proteomic databases

EPDⁱ	Q8R307
MaxQBⁱ	Q8R307
PaxDbⁱ	Q8R307
PeptideAtlasⁱ	Q8R307
PRIDEⁱ	Q8R307

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000037280; ENSMUSP00000036915; ENSMUSG00000034216
GeneIDⁱ	228545
KEGGⁱ	mmu:228545
UCSCⁱ	uc008lto.2 mouse

Organism-specific databases

CTDⁱ	57617
MGIⁱ	MGI:2443626 Vps18

Phylogenomic databases

eggNOGⁱ	KOG2034 Eukaryota ENOG410XRSD LUCA
GeneTreeⁱ	ENSGT00390000015233
HOVERGENⁱ	HBG057876
InParanoidⁱ	Q8R307
KOⁱ	K20181
OMAⁱ	DAWIEMG
OrthoDBⁱ	EOG091G01XI
PhylomeDBⁱ	Q8R307
TreeFamⁱ	TF105704

Miscellaneous databases

ChiTaRSⁱ	Vps18 mouse
Protein Ontology More...PROⁱ	PR:Q8R307
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000034216 Expressed in 212 organ(s), highest expression level in pancreas
CleanExⁱ	MM_VPS18
Genevisibleⁱ	Q8R307 MM

Family and domain databases

Gene3Dⁱ	1.25.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR000547 Clathrin_H-chain/VPS_repeat IPR007810 Pep3_Vps18 IPR011990 TPR-like_helical_dom_sf
Pfamⁱ	View protein in Pfam PF00637 Clathrin, 1 hit PF05131 Pep3_Vps18, 1 hit
PROSITEⁱ	View protein in PROSITE PS50236 CHCR, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	VPS18_MOUSE
Accessionⁱ	Q8R307Primary (citable) accession number: Q8R307 Secondary accession number(s): Q8BGV6, Q8BZX6, Q8C126
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
	Last sequence update:	June 7, 2004
	Last modified:	November 7, 2018
	This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8R307 (VPS18_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Vacuolar protein sorting-associated protein 18 homolog

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endosome

Lysosome

Other locations

Cytoskeleton

Endosome

Lysosome

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ