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Protein

Golgin-45

Gene

Blzf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.By similarity

Caution

Was initially thought to be a potential transcription factor, localized in the nucleus.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-162658 Golgi Cisternae Pericentriolar Stack Reorganization

Names & Taxonomyi

Protein namesi
Recommended name:
Golgin-45
Alternative name(s):
Basic leucine zipper nuclear factor 1
Gene namesi
Name:Blzf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1201607 Blzf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi393C → A: Reduced interaction with GORASP2; when associated with A-396. 1 Publication1
Mutagenesisi396C → A: Reduced interaction with GORASP2; when associated with A-393. 1 Publication1
Mutagenesisi403L → R: Reduced interaction with GORASP2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000875401 – 403Golgin-45Add BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineBy similarity1
Modified residuei356PhosphoserineBy similarity1

Post-translational modificationi

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.By similarity
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.By similarity

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8R2X8
PRIDEiQ8R2X8

PTM databases

iPTMnetiQ8R2X8
PhosphoSitePlusiQ8R2X8

Expressioni

Gene expression databases

BgeeiENSMUSG00000026577 Expressed in 281 organ(s), highest expression level in secondary oocyte
CleanExiMM_BLZF1
GenevisibleiQ8R2X8 MM

Interactioni

Subunit structurei

Interacts with GORASP2 (PubMed:11739401, PubMed:28049725). Interacts with the GTP-bound form of RAB2, but not with other Golgi Rab proteins (PubMed:11739401). Identified in a complex with RAB2 and GORASP2 (PubMed:11739401).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211404, 6 interactors
IntActiQ8R2X8, 8 interactors
MINTiQ8R2X8
STRINGi10090.ENSMUSP00000027866

Structurei

3D structure databases

ProteinModelPortaliQ8R2X8
SMRiQ8R2X8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni397 – 403Essential for interaction with GORASP21 Publication7

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili123 – 216Sequence analysisAdd BLAST94

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi22 – 26Tankyrase-binding motifBy similarity5

Domaini

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4074 Eukaryota
ENOG410YMPQ LUCA
GeneTreeiENSGT00390000009400
HOGENOMiHOG000088649
HOVERGENiHBG051750
InParanoidiQ8R2X8
OMAiYHFERMA
OrthoDBiEOG091G0JDO
PhylomeDBiQ8R2X8
TreeFamiTF317238

Family and domain databases

InterProiView protein in InterPro
IPR027095 Golgin-45
IPR013183 Hsk3-like
PANTHERiPTHR13066 PTHR13066, 1 hit
PfamiView protein in Pfam
PF08227 DASH_Hsk3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8R2X8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEKMTTLKSS ENKGILTSTP IRGAGDGMET EEPPKSVEVT HGVQPINQHV
60 70 80 90 100
LPSPRKKVSS DSPGVLQLGK ILNERTVEVE AVRIFVPKAA ITHDIPTKNT
110 120 130 140 150
KVKSLGHHRE ELHNQAEVVT DPRKELSEVK KVLEKLKNSE RRLLQDKEGL
160 170 180 190 200
SNQLRVQTEI NRELKKLLVA SVGDDPQYHF ERLAREKNQL ILENEALGRN
210 220 230 240 250
TAQLSEQLER MSIQCDVWRS KFLASRVMAD ELTNFRVVLQ RQNRDAQSAI
260 270 280 290 300
QDLLSEREQF RQEMTSTQKF LEELLVSLQW GREQTYSPNT QPHSTADLAL
310 320 330 340 350
TNHGLAQAIH AHLLGNVGIS HQKKIPTTVE FCSTPAEKMA EKVLRILDPV
360 370 380 390 400
ACTESSPDNQ FAESSPTTLL TTKKNIGRFH PYTRYENITF NCCNHCQGEL

IAL
Length:403
Mass (Da):45,541
Last modified:August 16, 2004 - v2
Checksum:iF87A9752AEEC3559
GO
Isoform 2 (identifier: Q8R2X8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     14-14: G → GKQEMMKG

Show »
Length:410
Mass (Da):46,374
Checksum:i59231CC8897468FB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti320S → R in AAH27025 (PubMed:15489334).Curated1
Sequence conflicti346I → N in AAH27025 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01118814G → GKQEMMKG in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005489 mRNA Translation: BAB24074.1
AK029787 mRNA Translation: BAC26617.1
AK167493 mRNA Translation: BAE39571.1
BC027025 mRNA Translation: AAH27025.1
CCDSiCCDS15435.1 [Q8R2X8-2]
CCDS48423.1 [Q8R2X8-1]
RefSeqiNP_001153680.1, NM_001160208.1 [Q8R2X8-1]
NP_001153681.1, NM_001160209.1 [Q8R2X8-1]
NP_079781.2, NM_025505.4 [Q8R2X8-2]
UniGeneiMm.33285

Genome annotation databases

EnsembliENSMUST00000027866; ENSMUSP00000027866; ENSMUSG00000026577 [Q8R2X8-2]
ENSMUST00000086032; ENSMUSP00000083196; ENSMUSG00000026577 [Q8R2X8-1]
ENSMUST00000120447; ENSMUSP00000113479; ENSMUSG00000026577 [Q8R2X8-1]
GeneIDi66352
KEGGimmu:66352
UCSCiuc007dig.1 mouse [Q8R2X8-2]
uc007dih.1 mouse [Q8R2X8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005489 mRNA Translation: BAB24074.1
AK029787 mRNA Translation: BAC26617.1
AK167493 mRNA Translation: BAE39571.1
BC027025 mRNA Translation: AAH27025.1
CCDSiCCDS15435.1 [Q8R2X8-2]
CCDS48423.1 [Q8R2X8-1]
RefSeqiNP_001153680.1, NM_001160208.1 [Q8R2X8-1]
NP_001153681.1, NM_001160209.1 [Q8R2X8-1]
NP_079781.2, NM_025505.4 [Q8R2X8-2]
UniGeneiMm.33285

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5H3JX-ray1.33B379-403[»]
ProteinModelPortaliQ8R2X8
SMRiQ8R2X8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211404, 6 interactors
IntActiQ8R2X8, 8 interactors
MINTiQ8R2X8
STRINGi10090.ENSMUSP00000027866

PTM databases

iPTMnetiQ8R2X8
PhosphoSitePlusiQ8R2X8

Proteomic databases

PaxDbiQ8R2X8
PRIDEiQ8R2X8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027866; ENSMUSP00000027866; ENSMUSG00000026577 [Q8R2X8-2]
ENSMUST00000086032; ENSMUSP00000083196; ENSMUSG00000026577 [Q8R2X8-1]
ENSMUST00000120447; ENSMUSP00000113479; ENSMUSG00000026577 [Q8R2X8-1]
GeneIDi66352
KEGGimmu:66352
UCSCiuc007dig.1 mouse [Q8R2X8-2]
uc007dih.1 mouse [Q8R2X8-1]

Organism-specific databases

CTDi8548
MGIiMGI:1201607 Blzf1

Phylogenomic databases

eggNOGiKOG4074 Eukaryota
ENOG410YMPQ LUCA
GeneTreeiENSGT00390000009400
HOGENOMiHOG000088649
HOVERGENiHBG051750
InParanoidiQ8R2X8
OMAiYHFERMA
OrthoDBiEOG091G0JDO
PhylomeDBiQ8R2X8
TreeFamiTF317238

Enzyme and pathway databases

ReactomeiR-MMU-162658 Golgi Cisternae Pericentriolar Stack Reorganization

Miscellaneous databases

PROiPR:Q8R2X8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026577 Expressed in 281 organ(s), highest expression level in secondary oocyte
CleanExiMM_BLZF1
GenevisibleiQ8R2X8 MM

Family and domain databases

InterProiView protein in InterPro
IPR027095 Golgin-45
IPR013183 Hsk3-like
PANTHERiPTHR13066 PTHR13066, 1 hit
PfamiView protein in Pfam
PF08227 DASH_Hsk3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGO45_MOUSE
AccessioniPrimary (citable) accession number: Q8R2X8
Secondary accession number(s): Q3TJC4, Q8C0U6, Q9DAV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 7, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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