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Protein

Aquaporin-3

Gene

Aqp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-MMU-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-MMU-432047 Passive transport by Aquaporins

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-3
Short name:
AQP-3
Alternative name(s):
Aquaglyceroporin-3
Gene namesi
Name:Aqp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1333777 Aqp3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28CytoplasmicSequence analysisAdd BLAST28
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
Topological domaini50 – 53ExtracellularSequence analysis4
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 109CytoplasmicSequence analysisAdd BLAST35
Transmembranei110 – 130HelicalSequence analysisAdd BLAST21
Topological domaini131 – 157ExtracellularSequence analysisAdd BLAST27
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Topological domaini179 – 188CytoplasmicSequence analysis10
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Topological domaini210 – 244ExtracellularSequence analysisAdd BLAST35
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266 – 292CytoplasmicSequence analysisAdd BLAST27

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice have dry skin with reduced SC (stratum corneum) hydration, decreased elasticity and impaired biosynthesis. The glycerol content of SC and epidermis is reduced, whereas that of dermis and serum is normal. The dry, relatively inelastic skin is probably related to the humectant properties of glycerol, and the impaired SC repair to impaired epidermal biosynthetic function.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000639441 – 292Aquaporin-3Add BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi141N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8R2N1
PRIDEiQ8R2N1

Expressioni

Tissue specificityi

Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes.

Gene expression databases

BgeeiENSMUSG00000028435 Expressed in 175 organ(s), highest expression level in cornea
CleanExiMM_AQP3
GenevisibleiQ8R2N1 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055110

Structurei

3D structure databases

ProteinModelPortaliQ8R2N1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi83 – 85NPA 13
Motifi215 – 217NPA 23

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0224 Eukaryota
COG0580 LUCA
GeneTreeiENSGT00510000046311
HOGENOMiHOG000288287
HOVERGENiHBG106057
InParanoidiQ8R2N1
KOiK09876
OMAiITSMLMG
OrthoDBiEOG091G0FMS
PhylomeDBiQ8R2N1
TreeFamiTF313173

Family and domain databases

CDDicd00333 MIP, 1 hit
Gene3Di1.20.1080.10, 1 hit
InterProiView protein in InterPro
IPR023271 Aquaporin-like
IPR023275 Aquaporin_3
IPR000425 MIP
IPR022357 MIP_CS
PfamiView protein in Pfam
PF00230 MIP, 1 hit
PRINTSiPR02015 AQUAPORIN3
PR00783 MINTRINSICP
SUPFAMiSSF81338 SSF81338, 1 hit
TIGRFAMsiTIGR00861 MIP, 1 hit
PROSITEiView protein in PROSITE
PS00221 MIP, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R2N1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGRQKELMNR CGEMLHIRYR LLRQALAECL GTLILVMFGC GSVAQVVLSR
60 70 80 90 100
GTHGGFLTIN LAFGFAVTLG ILVAGQVSGA HLNPAVTFAM CFLAREPWIK
110 120 130 140 150
LPIYALAQTL GAFLGAGIVF GLYYDAIWAF ANNELFVSGP NGTAGIFATY
160 170 180 190 200
PSGHLDMVNG FFDQFIGTAA LIVCVLAIVD PYNNPVPRGL EAFTVGLVVL
210 220 230 240 250
VIGTSMGFNS GYAVNPARDF GPRLFTALAG WGSEVFTTGR HWWWVPIVSP
260 270 280 290
LLGSIAGVFV YQLMIGCHLE QPPPSTEEEN VKLAHMKHKE QI
Length:292
Mass (Da):31,568
Last modified:June 1, 2002 - v1
Checksum:iE9A7FFFB43DD98C5
GO

Sequence cautioni

The sequence BAB03270 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170 – 173ALIV → PLL in AAD20304 (PubMed:12771381).Curated4
Sequence conflicti170 – 173ALIV → PLL in AAD20305 (PubMed:12771381).Curated4
Sequence conflicti214V → A in BAB03270 (PubMed:10777495).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104416 mRNA Translation: AAD20304.1
AF104417 Genomic DNA Translation: AAD20305.1
AK145678 mRNA Translation: BAE26584.1
AK160929 mRNA Translation: BAE36096.1
AL837521 Genomic DNA Translation: CAM19849.1
CH466538 Genomic DNA Translation: EDL05416.1
BC027400 mRNA Translation: AAH27400.1
AB019039 mRNA Translation: BAB03270.1 Different initiation.
CCDSiCCDS38714.1
RefSeqiNP_057898.2, NM_016689.2
UniGeneiMm.34043

Genome annotation databases

EnsembliENSMUST00000055327; ENSMUSP00000055110; ENSMUSG00000028435
GeneIDi11828
KEGGimmu:11828
UCSCiuc008sih.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104416 mRNA Translation: AAD20304.1
AF104417 Genomic DNA Translation: AAD20305.1
AK145678 mRNA Translation: BAE26584.1
AK160929 mRNA Translation: BAE36096.1
AL837521 Genomic DNA Translation: CAM19849.1
CH466538 Genomic DNA Translation: EDL05416.1
BC027400 mRNA Translation: AAH27400.1
AB019039 mRNA Translation: BAB03270.1 Different initiation.
CCDSiCCDS38714.1
RefSeqiNP_057898.2, NM_016689.2
UniGeneiMm.34043

3D structure databases

ProteinModelPortaliQ8R2N1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055110

Proteomic databases

PaxDbiQ8R2N1
PRIDEiQ8R2N1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055327; ENSMUSP00000055110; ENSMUSG00000028435
GeneIDi11828
KEGGimmu:11828
UCSCiuc008sih.1 mouse

Organism-specific databases

CTDi360
MGIiMGI:1333777 Aqp3

Phylogenomic databases

eggNOGiKOG0224 Eukaryota
COG0580 LUCA
GeneTreeiENSGT00510000046311
HOGENOMiHOG000288287
HOVERGENiHBG106057
InParanoidiQ8R2N1
KOiK09876
OMAiITSMLMG
OrthoDBiEOG091G0FMS
PhylomeDBiQ8R2N1
TreeFamiTF313173

Enzyme and pathway databases

ReactomeiR-MMU-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-MMU-432047 Passive transport by Aquaporins

Miscellaneous databases

PROiPR:Q8R2N1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028435 Expressed in 175 organ(s), highest expression level in cornea
CleanExiMM_AQP3
GenevisibleiQ8R2N1 MM

Family and domain databases

CDDicd00333 MIP, 1 hit
Gene3Di1.20.1080.10, 1 hit
InterProiView protein in InterPro
IPR023271 Aquaporin-like
IPR023275 Aquaporin_3
IPR000425 MIP
IPR022357 MIP_CS
PfamiView protein in Pfam
PF00230 MIP, 1 hit
PRINTSiPR02015 AQUAPORIN3
PR00783 MINTRINSICP
SUPFAMiSSF81338 SSF81338, 1 hit
TIGRFAMsiTIGR00861 MIP, 1 hit
PROSITEiView protein in PROSITE
PS00221 MIP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAQP3_MOUSE
AccessioniPrimary (citable) accession number: Q8R2N1
Secondary accession number(s): Q3TU75, Q9JJN8, Q9WTJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2002
Last modified: September 12, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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