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Protein

ERO1-like protein beta

Gene

Ero1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2, PDIA3, PDIA4, PDIA6 and NXNDC12. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell (By similarity). Involved in oxidative proinsulin folding in pancreatic cells, hence required for glucose homeostasis in vivo.By similarity1 Publication

Cofactori

FADBy similarity

Activity regulationi

Glutathione may be required to regulate its activity in the endoplasmic reticulum.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186FADBy similarity1
Binding sitei188FADBy similarity1
Binding sitei199FADBy similarity1
Binding sitei251FADBy similarity1
Binding sitei254FADBy similarity1
Binding sitei286FADBy similarity1
Binding sitei299FADBy similarity1

GO - Molecular functioni

  • oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor Source: InterPro
  • protein disulfide isomerase activity Source: GO_Central
  • protein disulfide oxidoreductase activity Source: MGI

GO - Biological processi

  • 4-hydroxyproline metabolic process Source: MGI
  • cell redox homeostasis Source: MGI
  • extracellular matrix organization Source: MGI
  • glucose homeostasis Source: MGI
  • insulin processing Source: MGI
  • protein folding in endoplasmic reticulum Source: GO_Central
  • protein maturation by protein folding Source: MGI

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-MMU-264876 Insulin processing

Names & Taxonomyi

Protein namesi
Recommended name:
ERO1-like protein beta (EC:1.8.4.-)
Short name:
ERO1-L-beta
Alternative name(s):
Endoplasmic reticulum oxidoreductase betaBy similarity
Endoplasmic reticulum oxidoreductin-1-like protein B
Oxidoreductin-1-L-beta
Gene namesi
Name:Ero1bBy similarity
Synonyms:Ero1lb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1914725 Ero1lb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi396C → A: Complete loss of disulfide oxidase activity in vitro. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000000841934 – 467ERO1-like protein betaAdd BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi81 ↔ 390By similarity
Disulfide bondi90 ↔ 130AlternateBy similarity
Disulfide bondi90 ↔ 95Redox-active; alternateBy similarity
Disulfide bondi100 ↔ 262By similarity
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi140N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi207 ↔ 240By similarity
Glycosylationi383N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi393 ↔ 396Redox-activeBy similarity

Post-translational modificationi

N-glycosylated.By similarity
The Cys-90/Cys-95 and Cys-393/Cys-396 disulfide bonds constitute the redox-active center. The Cys-90/Cys-95 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-393/Cys-396 (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8R2E9
MaxQBiQ8R2E9
PaxDbiQ8R2E9
PRIDEiQ8R2E9

PTM databases

iPTMnetiQ8R2E9
PhosphoSitePlusiQ8R2E9

Expressioni

Tissue specificityi

Predominantly expressed in stomach and pancreas (mostly in the islets of Langerhans) (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000057069 Expressed in 265 organ(s), highest expression level in islet of Langerhans
CleanExiMM_ERO1LB
ExpressionAtlasiQ8R2E9 baseline and differential
GenevisibleiQ8R2E9 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Heterodimer with ERO1A; disulfide-linked (By similarity). Also detected as monomer. Homodimers may be somewhat less active than monomers. The abundance of monomers and homodimers may be tissue-specific. Interacts with P4HB (By similarity). Interacts with ERP44 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8R2E9, 1 interactor
MINTiQ8R2E9
STRINGi10090.ENSMUSP00000071864

Structurei

3D structure databases

ProteinModelPortaliQ8R2E9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the EROs family.Curated

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

eggNOGiKOG2608 Eukaryota
COG5061 LUCA
GeneTreeiENSGT00390000007753
HOGENOMiHOG000012778
HOVERGENiHBG051507
InParanoidiQ8R2E9
KOiK10976
OMAiSISTHIC
OrthoDBiEOG091G04ZQ
PhylomeDBiQ8R2E9
TreeFamiTF314471

Family and domain databases

InterProiView protein in InterPro
IPR007266 Ero1
IPR037192 ERO1-like_sf
PANTHERiPTHR12613 PTHR12613, 1 hit
PfamiView protein in Pfam
PF04137 ERO1, 1 hit
PIRSFiPIRSF017205 ERO1, 1 hit
SUPFAMiSSF110019 SSF110019, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8R2E9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSPGFRRAVT GQGAAAAVQL LVTLSFLSSL VKTQVTGVLD DCLCDIDSID
60 70 80 90 100
KFNTYKIFPK IKKLQERDYF RYYKVNLKRP CPFWAEDGHC SIKDCHVEPC
110 120 130 140 150
PESKIPVGIK AGRSNKYSQA ANSTKELDDC EQANKLGAIN STLSNESKEA
160 170 180 190 200
FIDWARYDDS QDHFCELDDE RSPAAQYVDL LLNPERYTGY KGSSAWRVWN
210 220 230 240 250
SIYEENCFKP RSVYRPLNPL APSRGEDDGE SFYTWLEGLC LEKRVFYKLI
260 270 280 290 300
SGLHASINLH LCANYLLEET WGKPSWGPNI KEFRRRFDPV ETKGEGPRRL
310 320 330 340 350
KNLYFLYLIE LRALSKVAPY FERSIVDLYT GNVEDDADTK TLLLSIFQDT
360 370 380 390 400
KSFPMHFDEK SMFAGDKKGA KSLKEEFRLH FKNISRIMDC VGCDKCRLWG
410 420 430 440 450
KLQTQGLGTA LKILFSEKEI QNLPENSPSK GFQLTRQEIV ALLNAFGRLS
460
TSIRELQNFK ALLQHRR
Length:467
Mass (Da):53,518
Last modified:June 1, 2002 - v1
Checksum:i442E7D6CB42A2446
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1Y7VNF4A0A1Y7VNF4_MOUSE
ERO1-like beta (S. cerevisiae)
Ero1lb
493Annotation score:
A0A1Y7VJM4A0A1Y7VJM4_MOUSE
ERO1-like beta (S. cerevisiae)
Ero1lb
485Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF489856 mRNA Translation: AAL96670.1
AK041951 mRNA Translation: BAC31108.1
BC058721 mRNA Translation: AAH58721.1
BC112426 mRNA Translation: AAI12427.1
BC145888 mRNA Translation: AAI45889.1
BC145890 mRNA Translation: AAI45891.1
CCDSiCCDS36594.1
RefSeqiNP_080460.2, NM_026184.2
UniGeneiMm.358706

Genome annotation databases

EnsembliENSMUST00000071973; ENSMUSP00000071864; ENSMUSG00000057069
GeneIDi67475
KEGGimmu:67475
UCSCiuc007plr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF489856 mRNA Translation: AAL96670.1
AK041951 mRNA Translation: BAC31108.1
BC058721 mRNA Translation: AAH58721.1
BC112426 mRNA Translation: AAI12427.1
BC145888 mRNA Translation: AAI45889.1
BC145890 mRNA Translation: AAI45891.1
CCDSiCCDS36594.1
RefSeqiNP_080460.2, NM_026184.2
UniGeneiMm.358706

3D structure databases

ProteinModelPortaliQ8R2E9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R2E9, 1 interactor
MINTiQ8R2E9
STRINGi10090.ENSMUSP00000071864

PTM databases

iPTMnetiQ8R2E9
PhosphoSitePlusiQ8R2E9

Proteomic databases

EPDiQ8R2E9
MaxQBiQ8R2E9
PaxDbiQ8R2E9
PRIDEiQ8R2E9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071973; ENSMUSP00000071864; ENSMUSG00000057069
GeneIDi67475
KEGGimmu:67475
UCSCiuc007plr.1 mouse

Organism-specific databases

CTDi67475
MGIiMGI:1914725 Ero1lb

Phylogenomic databases

eggNOGiKOG2608 Eukaryota
COG5061 LUCA
GeneTreeiENSGT00390000007753
HOGENOMiHOG000012778
HOVERGENiHBG051507
InParanoidiQ8R2E9
KOiK10976
OMAiSISTHIC
OrthoDBiEOG091G04ZQ
PhylomeDBiQ8R2E9
TreeFamiTF314471

Enzyme and pathway databases

ReactomeiR-MMU-264876 Insulin processing

Miscellaneous databases

ChiTaRSiEro1lb mouse
PROiPR:Q8R2E9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057069 Expressed in 265 organ(s), highest expression level in islet of Langerhans
CleanExiMM_ERO1LB
ExpressionAtlasiQ8R2E9 baseline and differential
GenevisibleiQ8R2E9 MM

Family and domain databases

InterProiView protein in InterPro
IPR007266 Ero1
IPR037192 ERO1-like_sf
PANTHERiPTHR12613 PTHR12613, 1 hit
PfamiView protein in Pfam
PF04137 ERO1, 1 hit
PIRSFiPIRSF017205 ERO1, 1 hit
SUPFAMiSSF110019 SSF110019, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiERO1B_MOUSE
AccessioniPrimary (citable) accession number: Q8R2E9
Secondary accession number(s): Q14DN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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