UniProtKB - Q8R1Q9 (RBSK_MOUSE)
Protein
Ribokinase
Gene
Rbks
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.UniRule annotation
Catalytic activityi
- EC:2.7.1.15UniRule annotation
Cofactori
Mg2+UniRule annotationNote: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.UniRule annotation
Activity regulationi
Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity. Competitively inhibited by phosphonoacetic acid, etidronate, 2-carboxethylphosphonic acid, N-(phosphonomethyl)glycine, N-(phosphonomethyl)iminodiacetic acid and clodronate (By similarity).UniRule annotationBy similarity
: D-ribose degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Ribokinase (Rbks)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose, the pathway D-ribose degradation and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 155 | SubstrateUniRule annotation | 1 | |
Binding sitei | 200 | ATPUniRule annotation | 1 | |
Binding sitei | 257 | ATPUniRule annotation | 1 | |
Metal bindingi | 264 | PotassiumUniRule annotation | 1 | |
Metal bindingi | 266 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 270 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 270 | SubstrateUniRule annotation | 1 | |
Binding sitei | 296 | ATPUniRule annotation | 1 | |
Metal bindingi | 302 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 305 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 307 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 311 | PotassiumUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 236 – 241 | ATPUniRule annotation | 6 | |
Nucleotide bindingi | 269 – 270 | ATPUniRule annotation | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- ribokinase activity Source: UniProtKB-UniRule
GO - Biological processi
- D-ribose catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium |
Enzyme and pathway databases
Reactomei | R-MMU-71336, Pentose phosphate pathway |
UniPathwayi | UPA00916;UER00889 |
Names & Taxonomyi
Protein namesi | Recommended name: RibokinaseUniRule annotationImported (EC:2.7.1.15UniRule annotation)Short name: RKUniRule annotation |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1918586, Rbks |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000375977 | 1 – 323 | RibokinaseAdd BLAST | 323 |
Proteomic databases
EPDi | Q8R1Q9 |
MaxQBi | Q8R1Q9 |
PaxDbi | Q8R1Q9 |
PeptideAtlasi | Q8R1Q9 |
PRIDEi | Q8R1Q9 |
PTM databases
iPTMneti | Q8R1Q9 |
PhosphoSitePlusi | Q8R1Q9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029136, Expressed in adult mammalian kidney and 162 other tissues |
Genevisiblei | Q8R1Q9, MM |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000031018 |
Miscellaneous databases
RNActi | Q8R1Q9, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 26 – 28 | Substrate bindingUniRule annotation | 3 | |
Regioni | 54 – 58 | Substrate bindingUniRule annotation | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2855, Eukaryota |
GeneTreei | ENSGT00390000005743 |
HOGENOMi | CLU_027634_2_3_1 |
InParanoidi | Q8R1Q9 |
OMAi | DIVLIQQ |
OrthoDBi | 918144at2759 |
PhylomeDBi | Q8R1Q9 |
TreeFami | TF105770 |
Family and domain databases
CDDi | cd01174, ribokinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01987, Ribokinase, 1 hit |
InterProi | View protein in InterPro IPR002173, Carboh/pur_kinase_PfkB_CS IPR011877, D_ribokin IPR011611, PfkB_dom IPR002139, Ribo/fructo_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00990, RIBOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR02152, D_ribokin_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00584, PFKB_KINASES_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8R1Q9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAACGEPGRP WQEEEAAAVV VVGSCMTDLV SLTSRLPKTG ETIHGHEFFI
60 70 80 90 100
GFGGKGANQC VQAARLGAKA AIVCKVGNDS FGNDYIENLK QNHISTEFTY
110 120 130 140 150
QTRDAATGTA SIIVNNEGQN IIVIVAGANL FLNSEDLKKA ASVISRAKVM
160 170 180 190 200
ICQLEISPAA SLEALTMARR SGVKTLFNPA PAMADLDPQF YTLSSIFCCN
210 220 230 240 250
ESEAEILTGH AVSDPTTAGK AAMILLERGC QVVVITLGAS GCVILSQAEP
260 270 280 290 300
VPKHIPTEAV KAVDTTGAGD SFVGALAFYL AYYPNLSLEE MLKRSNFIAA
310 320
VSVQATGTQS SYPYKKDLPL ALF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK138939 mRNA Translation: BAE23825.1 BC023339 mRNA Translation: AAH23339.1 |
CCDSi | CCDS19187.1 |
RefSeqi | NP_694876.1, NM_153196.1 |
Genome annotation databases
Ensembli | ENSMUST00000031018; ENSMUSP00000031018; ENSMUSG00000029136 |
GeneIDi | 71336 |
KEGGi | mmu:71336 |
UCSCi | uc008wyv.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK138939 mRNA Translation: BAE23825.1 BC023339 mRNA Translation: AAH23339.1 |
CCDSi | CCDS19187.1 |
RefSeqi | NP_694876.1, NM_153196.1 |
3D structure databases
SMRi | Q8R1Q9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000031018 |
PTM databases
iPTMneti | Q8R1Q9 |
PhosphoSitePlusi | Q8R1Q9 |
Proteomic databases
EPDi | Q8R1Q9 |
MaxQBi | Q8R1Q9 |
PaxDbi | Q8R1Q9 |
PeptideAtlasi | Q8R1Q9 |
PRIDEi | Q8R1Q9 |
Protocols and materials databases
Antibodypediai | 13898, 134 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000031018; ENSMUSP00000031018; ENSMUSG00000029136 |
GeneIDi | 71336 |
KEGGi | mmu:71336 |
UCSCi | uc008wyv.1, mouse |
Organism-specific databases
CTDi | 64080 |
MGIi | MGI:1918586, Rbks |
Phylogenomic databases
eggNOGi | KOG2855, Eukaryota |
GeneTreei | ENSGT00390000005743 |
HOGENOMi | CLU_027634_2_3_1 |
InParanoidi | Q8R1Q9 |
OMAi | DIVLIQQ |
OrthoDBi | 918144at2759 |
PhylomeDBi | Q8R1Q9 |
TreeFami | TF105770 |
Enzyme and pathway databases
UniPathwayi | UPA00916;UER00889 |
Reactomei | R-MMU-71336, Pentose phosphate pathway |
Miscellaneous databases
BioGRID-ORCSi | 71336, 1 hit in 18 CRISPR screens |
ChiTaRSi | Rbks, mouse |
PROi | PR:Q8R1Q9 |
RNActi | Q8R1Q9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029136, Expressed in adult mammalian kidney and 162 other tissues |
Genevisiblei | Q8R1Q9, MM |
Family and domain databases
CDDi | cd01174, ribokinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01987, Ribokinase, 1 hit |
InterProi | View protein in InterPro IPR002173, Carboh/pur_kinase_PfkB_CS IPR011877, D_ribokin IPR011611, PfkB_dom IPR002139, Ribo/fructo_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00990, RIBOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR02152, D_ribokin_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00584, PFKB_KINASES_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RBSK_MOUSE | |
Accessioni | Q8R1Q9Primary (citable) accession number: Q8R1Q9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 26, 2009 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families