Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeodomain-only protein

Gene

Hopx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (PubMed:12297045, PubMed:12297046). Acts as a co-chaperone for HSPA1A and HSPA1B chaperone proteins and assists in chaperone-mediated protein refolding (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi3 – 62Homeobox; atypicalPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Homeodomain-only protein
Alternative name(s):
Homeobox-only protein
Odd homeobox protein 1
Short name:
mOB1
Gene namesi
Name:Hopx
Synonyms:Hod, Hop, Ob1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1916782 Hopx

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display partial embryonic lethality and heart failure.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi35 – 39LIAAE → AAASM in H2; induces a strong reduction in interaction with SRF and subsequent ability to prevent transcription of cardiac-specific genes. 1 Publication5
Mutagenesisi49 – 53KWFKQ → AAASM in H3; does not affect the interaction with SRF and keeps its ability to prevent transcription of cardiac-specific genes. 1 Publication5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000491301 – 73Homeodomain-only proteinAdd BLAST73

Proteomic databases

EPDiQ8R1H0
MaxQBiQ8R1H0
PaxDbiQ8R1H0
PeptideAtlasiQ8R1H0
PRIDEiQ8R1H0

PTM databases

iPTMnetiQ8R1H0
PhosphoSitePlusiQ8R1H0

Expressioni

Tissue specificityi

Expressed in the embryonic and adult heart and in the adult brain, liver, lung, skeletal muscle, intestine and spleen. Throughout embryonic and postnatal development, it is expressed in the myocardium.3 Publications

Developmental stagei

First detected at E7.75 in trophoblasts within extraembryonic membranes, in the lateral wings of the cardiac crescent and in the anterior head folds. At E8.0, it is expressed along the length of the linear heart tube and in the head folds. Expressed throughout the myocardium at E9.5 and in the branchial arches. At E12.5, it is expressed in the heart and in the ventricular zone of the neural tube. At E13.5, it is weakly expressed in the intestinal epithelium. At E13.5 and E15.5, it is also expressed in skeletal muscle, stratified epithelium (upper aerodigestive tract and skin), epithelium of developing airways, vibrissae, midbrain/hindbrain junction, meninges, mesenchymal cellular condensations that preceded cartilage formation and chondrocytes.3 Publications

Inductioni

By the transcription factor NKX2-5 that acts as a direct regulator.1 Publication

Gene expression databases

BgeeiENSMUSG00000059325 Expressed in 296 organ(s), highest expression level in right lung
CleanExiMM_HOPX
GenevisibleiQ8R1H0 MM

Interactioni

Subunit structurei

Interacts with serum response factor (SRF) (PubMed:12297045, PubMed:12297046). Component of a large complex containing histone deacetylases such as HDAC2 (PubMed:12975471). Interacts with the acetylated forms of HSPA1A and HSPA1B. Interacts with HSPA8 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Smad4P974712EBI-6913924,EBI-5259270

Protein-protein interaction databases

BioGridi216662, 2 interactors
DIPiDIP-61663N
IntActiQ8R1H0, 10 interactors
MINTiQ8R1H0
STRINGi10090.ENSMUSP00000080630

Structurei

Secondary structure

173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8R1H0
SMRiQ8R1H0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R1H0

Family & Domainsi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0490 Eukaryota
ENOG410YIJ3 LUCA
GeneTreeiENSGT00390000017143
HOGENOMiHOG000112932
HOVERGENiHBG051921
InParanoidiQ8R1H0
OMAiKVSKHPD
OrthoDBiEOG091G1BXE
PhylomeDBiQ8R1H0
TreeFamiTF330730

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR039162 HOPX
PANTHERiPTHR21408 PTHR21408, 1 hit
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS50071 HOMEOBOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R1H0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSAQTASGPT EDQVEILEYN FNKVNKHPDP TTLCLIAAEA GLTEEQTQKW
60 70
FKQRLAEWRR SEGLPSECRS VTD
Length:73
Mass (Da):8,282
Last modified:June 1, 2002 - v1
Checksum:i9D9B6D7F7A11C592
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6A → P in BAC25235 (PubMed:16141072).Curated1
Sequence conflicti6A → V in AAN16320 (PubMed:12297045).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF536202 mRNA Translation: AAN16320.1
AF534182 mRNA Translation: AAN05634.1
AF492703 mRNA Translation: AAM46823.1
AF492704 mRNA Translation: AAM46824.1
AK003784 mRNA Translation: BAC25054.1
AK004798 mRNA Translation: BAC25097.1
AK008297 mRNA Translation: BAC25214.1
AK008439 mRNA Translation: BAC25219.1
AK009007 mRNA Translation: BAC25235.1
BC024546 mRNA Translation: AAH24546.1
CCDSiCCDS39120.1
RefSeqiNP_001153372.1, NM_001159900.1
NP_001153373.1, NM_001159901.1
NP_783199.1, NM_175606.3
UniGeneiMm.181852
Mm.478741

Genome annotation databases

EnsembliENSMUST00000081964; ENSMUSP00000080630; ENSMUSG00000059325
ENSMUST00000113453; ENSMUSP00000109080; ENSMUSG00000059325
ENSMUST00000120827; ENSMUSP00000113295; ENSMUSG00000059325
GeneIDi74318
KEGGimmu:74318
UCSCiuc008xvv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF536202 mRNA Translation: AAN16320.1
AF534182 mRNA Translation: AAN05634.1
AF492703 mRNA Translation: AAM46823.1
AF492704 mRNA Translation: AAM46824.1
AK003784 mRNA Translation: BAC25054.1
AK004798 mRNA Translation: BAC25097.1
AK008297 mRNA Translation: BAC25214.1
AK008439 mRNA Translation: BAC25219.1
AK009007 mRNA Translation: BAC25235.1
BC024546 mRNA Translation: AAH24546.1
CCDSiCCDS39120.1
RefSeqiNP_001153372.1, NM_001159900.1
NP_001153373.1, NM_001159901.1
NP_783199.1, NM_175606.3
UniGeneiMm.181852
Mm.478741

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UHSNMR-A10-73[»]
2HI3NMR-A1-73[»]
ProteinModelPortaliQ8R1H0
SMRiQ8R1H0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216662, 2 interactors
DIPiDIP-61663N
IntActiQ8R1H0, 10 interactors
MINTiQ8R1H0
STRINGi10090.ENSMUSP00000080630

PTM databases

iPTMnetiQ8R1H0
PhosphoSitePlusiQ8R1H0

Proteomic databases

EPDiQ8R1H0
MaxQBiQ8R1H0
PaxDbiQ8R1H0
PeptideAtlasiQ8R1H0
PRIDEiQ8R1H0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081964; ENSMUSP00000080630; ENSMUSG00000059325
ENSMUST00000113453; ENSMUSP00000109080; ENSMUSG00000059325
ENSMUST00000120827; ENSMUSP00000113295; ENSMUSG00000059325
GeneIDi74318
KEGGimmu:74318
UCSCiuc008xvv.2 mouse

Organism-specific databases

CTDi84525
MGIiMGI:1916782 Hopx

Phylogenomic databases

eggNOGiKOG0490 Eukaryota
ENOG410YIJ3 LUCA
GeneTreeiENSGT00390000017143
HOGENOMiHOG000112932
HOVERGENiHBG051921
InParanoidiQ8R1H0
OMAiKVSKHPD
OrthoDBiEOG091G1BXE
PhylomeDBiQ8R1H0
TreeFamiTF330730

Miscellaneous databases

EvolutionaryTraceiQ8R1H0
PROiPR:Q8R1H0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059325 Expressed in 296 organ(s), highest expression level in right lung
CleanExiMM_HOPX
GenevisibleiQ8R1H0 MM

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR039162 HOPX
PANTHERiPTHR21408 PTHR21408, 1 hit
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS50071 HOMEOBOX_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHOP_MOUSE
AccessioniPrimary (citable) accession number: Q8R1H0
Secondary accession number(s): Q8C1N5, Q8CJ22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again