UniProtKB - Q8R1B8 (RORB_MOUSE)
Protein
Nuclear receptor ROR-beta
Gene
Rorb
Organism
Mus musculus (Mouse)
Status
Functioni
Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Considered to have intrinsic transcriptional activity, have some natural ligands such as all-trans retinoic acid (ATRA) and other retinoids which act as inverse agonists repressing the transcriptional activity. Required for normal postnatal development of rod and cone photoreceptor cells. Modulates rod photoreceptors differentiation at least by inducing the transcription factor NRL-mediated pathway. In cone photoreceptor cells, regulates transcription of OPN1SW. Involved in the regulation of the period length and stability of the circadian rhythm. May control cytoarchitectural patterning of neocortical neurons during development. May act in a dose-dependent manner to regulate barrel formation upon innervation of layer IV neurons by thalamocortical axons. May play a role in the suppression of osteoblastic differentiation through the inhibition of RUNX2 transcriptional activity.By similarity
Isoform 1 is critical for hindlimb motor control and for the differentiation of amacrine and horizontal cells in the retina. Regulates the expression of PTF1A synergistically with FOXN4.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 18 – 93 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 76 | |
Zinc fingeri | 21 – 41 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 57 – 81 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- melatonin receptor activity Source: MGI
- nuclear receptor activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
- transcription factor binding Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- amacrine cell differentiation Source: UniProtKB
- cellular response to retinoic acid Source: UniProtKB
- eye photoreceptor cell development Source: UniProtKB
- negative regulation of osteoblast differentiation Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of circadian rhythm Source: UniProtKB
- retina development in camera-type eye Source: UniProtKB
- retinal cone cell development Source: UniProtKB
- retinal rod cell development Source: UniProtKB
- rhythmic process Source: UniProtKB-KW
- visual perception Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding, Receptor |
Biological process | Biological rhythms, Sensory transduction, Transcription, Transcription regulation, Vision |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear receptor ROR-betaAlternative name(s): Nuclear receptor RZR-beta Nuclear receptor subfamily 1 group F member 2 Retinoid-related orphan receptor-beta |
Gene namesi | Name:Rorb Synonyms:Nr1f2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1343464, Rorb |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
- nucleoplasm By similarity
Nucleus
- nucleoplasm Source: UniProtKB
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mice are blind, show juvenile ataxia, duck gait, hind paw clasping reflex as well as delayed onset of male fertility. They suffer a degeneration of the retina during postnatal development with severe defects in photoreceptor cell morphology. Mice display reduced anxiety and learned helplessness-related behaviors. They also show a significant increase of the circadian period. Knockouts for isoform 1 display duck gait and lack amacrine and horizontal cells with an excess of ganglion cells in the retina (PubMed:23652001).5 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053515 | 1 – 470 | Nuclear receptor ROR-betaAdd BLAST | 470 |
Proteomic databases
PaxDbi | Q8R1B8 |
PRIDEi | Q8R1B8 |
PTM databases
iPTMneti | Q8R1B8 |
PhosphoSitePlusi | Q8R1B8 |
Expressioni
Tissue specificityi
Expressed in inner and outer neuroblastic layer as well as in the ganglion cell layer of the developing retina. Expressed in bone marrow osteoprogenitor cells.5 Publications
Developmental stagei
Expressed in the lateral cortical plate at 18.5 dpc and only very weakly expressed by cortical neurons at 15.5 dpc. Expression progressively increases in layer IV neurons of the prospective somatosensory, visual and auditory cortices. At much lower levels, also expressed by scattered neurons in layer V in these areas. By P4, expression is striking in the whisker barrel cortex of the somatosensory cortex. During retinal development, isoform 1 is broadly expressed in between 13.5 dpc and P5 then declines and is maintained at lower levels into adulthood. At 15.5 dpc, is expressed in the immature cochlea, brainstem, spinal cord and cerebral cortex. Isoform 2 is first detected at low levels at late embryonic stages (18.5 dpc), the expression highly increases during the first postnatal week and is maintained during the adulthood.2 Publications
Inductioni
Induced by aging in bone marrow.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000036192, Expressed in visual cortex and 178 other tissues |
ExpressionAtlasi | Q8R1B8, baseline and differential |
Genevisiblei | Q8R1B8, MM |
Interactioni
Subunit structurei
Monomer.
Interacts with CRX.
By similarityGO - Molecular functioni
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 230457, 8 interactors |
STRINGi | 10090.ENSMUSP00000047597 |
Miscellaneous databases
RNActi | Q8R1B8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 222 – 460 | NR LBDPROSITE-ProRule annotationAdd BLAST | 239 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 456 – 461 | AF-2 | 6 |
Domaini
AF-2 (activation function-2) motif is required for recruiting coregulators containing the LXXLL motif, such as NCOA1, and control the transactivational activity.By similarity
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 21 – 41 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 57 – 81 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4216, Eukaryota |
GeneTreei | ENSGT00940000157708 |
HOGENOMi | CLU_007368_2_0_1 |
InParanoidi | Q8R1B8 |
OMAi | RECVHAR |
OrthoDBi | 583704at2759 |
PhylomeDBi | Q8R1B8 |
TreeFami | TF319910 |
Family and domain databases
CDDi | cd06968, NR_DBD_ROR, 1 hit |
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR044101, NR_DBD_ROR IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR003079, ROR_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR01293, RORNUCRECPTR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative promoter usage. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 2 (identifier: Q8R1B8-2) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MCENQPKTKA DGTAQIEVIP CKICGDKSSG IHYGVITCEG CKGFFRRSQQ
60 70 80 90 100
NNASYSCPRQ RNCLIDRTNR NRCQHCRLQK CLALGMSRDA VKFGRMSKKQ
110 120 130 140 150
RDSLYAEVQK HQQRLQEQRQ QQSGEAEALA RVYSSSISNG LSNLNTETGG
160 170 180 190 200
TYANGHVIDL PKSEGYYSID SGQPSPDQSG LDMTGIKQIK QEPIYDLTSV
210 220 230 240 250
PNLFTYSSFN NGQLAPGITM SEIDRIAQNI IKSHLETCQY TMEELHQLAW
260 270 280 290 300
QTHTYEEIKA YQSKSREALW QQCAIQITHA IQYVVEFAKR ITGFMELCQN
310 320 330 340 350
DQILLLKSGC LEVVLVRMCR AFNPLNNTVL FEGKYGGMQM FKALGSDDLV
360 370 380 390 400
NEAFDFAKNL CSLQLTEEEI ALFSSAVLIS PDRAWLIEPR KVQKLQEKIY
410 420 430 440 450
FALQHVIQKN HLDDETLAKL IAKIPTITAV CNLHGEKLQV FKQSHPDIVN
460 470
TLFPPLYKEL FNPDCAAVCK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J1H4 | A0A0R4J1H4_MOUSE | Nuclear receptor ROR-beta | Rorb | 385 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 107 | E → G in BAE93688 (Ref. 2) Curated | 1 | |
Sequence conflicti | 220 | M → L in BAE93688 (Ref. 2) Curated | 1 | |
Sequence conflicti | 247 | Q → H in BAE93688 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_022576 | 1 – 13 | MCENQ…KADGT → MR in isoform 1. 2 PublicationsAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC024842 mRNA Translation: AAH24842.2 Sequence problems. BC058269 mRNA Translation: AAH58269.1 Sequence problems. DQ779923 mRNA Translation: ABG77269.1 DQ779924 mRNA Translation: ABG77270.1 AB258405 mRNA Translation: BAE93688.1 |
CCDSi | CCDS37932.1 [Q8R1B8-1] CCDS37933.1 [Q8R1B8-2] |
RefSeqi | NP_001036819.1, NM_001043354.2 [Q8R1B8-1] NP_001276850.1, NM_001289921.1 NP_666207.3, NM_146095.4 [Q8R1B8-2] |
Genome annotation databases
Ensembli | ENSMUST00000040153; ENSMUSP00000047597; ENSMUSG00000036192 [Q8R1B8-2] ENSMUST00000112832; ENSMUSP00000108451; ENSMUSG00000036192 [Q8R1B8-1] |
GeneIDi | 225998 |
KEGGi | mmu:225998 |
UCSCi | uc008gyc.2, mouse [Q8R1B8-2] uc008gye.2, mouse [Q8R1B8-1] |
Keywords - Coding sequence diversityi
Alternative promoter usageSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC024842 mRNA Translation: AAH24842.2 Sequence problems. BC058269 mRNA Translation: AAH58269.1 Sequence problems. DQ779923 mRNA Translation: ABG77269.1 DQ779924 mRNA Translation: ABG77270.1 AB258405 mRNA Translation: BAE93688.1 |
CCDSi | CCDS37932.1 [Q8R1B8-1] CCDS37933.1 [Q8R1B8-2] |
RefSeqi | NP_001036819.1, NM_001043354.2 [Q8R1B8-1] NP_001276850.1, NM_001289921.1 NP_666207.3, NM_146095.4 [Q8R1B8-2] |
3D structure databases
SMRi | Q8R1B8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230457, 8 interactors |
STRINGi | 10090.ENSMUSP00000047597 |
PTM databases
iPTMneti | Q8R1B8 |
PhosphoSitePlusi | Q8R1B8 |
Proteomic databases
PaxDbi | Q8R1B8 |
PRIDEi | Q8R1B8 |
Protocols and materials databases
Antibodypediai | 1699, 384 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000040153; ENSMUSP00000047597; ENSMUSG00000036192 [Q8R1B8-2] ENSMUST00000112832; ENSMUSP00000108451; ENSMUSG00000036192 [Q8R1B8-1] |
GeneIDi | 225998 |
KEGGi | mmu:225998 |
UCSCi | uc008gyc.2, mouse [Q8R1B8-2] uc008gye.2, mouse [Q8R1B8-1] |
Organism-specific databases
CTDi | 6096 |
MGIi | MGI:1343464, Rorb |
Phylogenomic databases
eggNOGi | KOG4216, Eukaryota |
GeneTreei | ENSGT00940000157708 |
HOGENOMi | CLU_007368_2_0_1 |
InParanoidi | Q8R1B8 |
OMAi | RECVHAR |
OrthoDBi | 583704at2759 |
PhylomeDBi | Q8R1B8 |
TreeFami | TF319910 |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Miscellaneous databases
BioGRID-ORCSi | 225998, 1 hit in 17 CRISPR screens |
ChiTaRSi | Rorb, mouse |
PROi | PR:Q8R1B8 |
RNActi | Q8R1B8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036192, Expressed in visual cortex and 178 other tissues |
ExpressionAtlasi | Q8R1B8, baseline and differential |
Genevisiblei | Q8R1B8, MM |
Family and domain databases
CDDi | cd06968, NR_DBD_ROR, 1 hit |
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR044101, NR_DBD_ROR IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR003079, ROR_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR01293, RORNUCRECPTR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RORB_MOUSE | |
Accessioni | Q8R1B8Primary (citable) accession number: Q8R1B8 Secondary accession number(s): Q0PQZ2, Q0PQZ3, Q1MVR9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 21, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families