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Protein

ERO1-like protein alpha

Gene

Ero1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins (By similarity). Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.By similarity2 Publications

Cofactori

FADBy similarity

Activity regulationi

Enzyme activity is tightly regulated to prevent the accumulation of reactive oxygen species in the endoplasmic reticulum. Reversibly down-regulated by the formation of disulfide bonds between the active site Cys-94 and Cys-130, and between Cys-99 and Cys-104. Glutathione may be required to regulate its activity in the endoplasmic reticulum (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186FADBy similarity1
Binding sitei188FADBy similarity1
Binding sitei199FADBy similarity1
Binding sitei248FADBy similarity1
Binding sitei251FADBy similarity1
Binding sitei283FADBy similarity1
Binding sitei296FADBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processApoptosis, Electron transport, Transport
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-MMU-264876 Insulin processing
R-MMU-3299685 Detoxification of Reactive Oxygen Species

Names & Taxonomyi

Protein namesi
Recommended name:
ERO1-like protein alpha (EC:1.8.4.-)
Short name:
ERO1-L
Short name:
ERO1-L-alpha
Alternative name(s):
Endoplasmic reticulum oxidoreductase alphaBy similarity
Endoplasmic reticulum oxidoreductin-1-like protein
Oxidoreductin-1-L-alpha
Gene namesi
Name:Ero1aBy similarity
Synonyms:Ero1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1354385 Ero1l

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000000841624 – 464ERO1-like protein alphaAdd BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi35 ↔ 48By similarity
Disulfide bondi37 ↔ 46By similarity
Disulfide bondi85 ↔ 387By similarity
Disulfide bondi94 ↔ 130AlternateBy similarity
Disulfide bondi94 ↔ 99Redox-active; alternateBy similarity
Disulfide bondi99 ↔ 104AlternateBy similarity
Modified residuei106PhosphoserineBy similarity1
Modified residuei142PhosphoserineBy similarity1
Disulfide bondi207 ↔ 237By similarity
Glycosylationi276N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi380N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi390 ↔ 393Redox-activeBy similarity

Post-translational modificationi

N-glycosylated.By similarity
The Cys-94/Cys-99 and Cys-390/Cys-393 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-390/Cys-393. The regulatory Cys-99/Cys-104 disulfide bond stabilizes the other regulatory bond Cys-94/Cys-130 (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8R180
MaxQBiQ8R180
PaxDbiQ8R180
PRIDEiQ8R180

PTM databases

iPTMnetiQ8R180
PhosphoSitePlusiQ8R180
SwissPalmiQ8R180

Expressioni

Tissue specificityi

Widely expressed (at protein level).1 Publication

Inductioni

Stimulated by hypoxia; suggesting that it is regulated via the HIF-pathway. By ER stress in a DDIT3/CHOP-dependent manner.2 Publications

Gene expression databases

BgeeiENSMUSG00000021831 Expressed in 285 organ(s), highest expression level in multi-cellular organism
CleanExiMM_ERO1L
ExpressionAtlasiQ8R180 baseline and differential
GenevisibleiQ8R180 MM

Interactioni

Subunit structurei

Predominantly monomer. May function both as a monomer and a homodimer. Interacts with PDILT (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8R180, 1 interactor
MINTiQ8R180
STRINGi10090.ENSMUSP00000022378

Structurei

3D structure databases

ProteinModelPortaliQ8R180
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the EROs family.Curated

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

eggNOGiKOG2608 Eukaryota
COG5061 LUCA
GeneTreeiENSGT00390000007753
HOGENOMiHOG000012778
HOVERGENiHBG051507
InParanoidiQ8R180
KOiK10950
OMAiMDCVGCL
OrthoDBiEOG091G04ZQ
PhylomeDBiQ8R180
TreeFamiTF314471

Family and domain databases

InterProiView protein in InterPro
IPR007266 Ero1
IPR037192 ERO1-like_sf
PANTHERiPTHR12613 PTHR12613, 1 hit
PfamiView protein in Pfam
PF04137 ERO1, 1 hit
PIRSFiPIRSF017205 ERO1, 1 hit
SUPFAMiSSF110019 SSF110019, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8R180-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGRAWGLLVG LLGVVWLLRL GHGEERRPET AAQRCFCQVS GYLDDCTCDV
60 70 80 90 100
ETIDKFNNYR LFPRLQKLLE SDYFRYYKVN LKKPCPFWND INQCGRRDCA
110 120 130 140 150
VKPCHSDEVP DGIKSASYKY SEEANRIEEC EQAERLGAVD ESLSEETQKA
160 170 180 190 200
VLQWTKHDDS SDSFCEIDDI QSPDAEYVDL LLNPERYTGY KGPDAWRIWS
210 220 230 240 250
VIYEENCFKP QTIQRPLASG RGKSKENTFY NWLEGLCVEK RAFYRLISGL
260 270 280 290 300
HASINVHLSA RYLLQDTWLE KKWGHNVTEF QQRFDGILTE GEGPRRLRNL
310 320 330 340 350
YFLYLIELRA LSKVLPFFER PDFQLFTGNK VQDAENKALL LEILHEIKSF
360 370 380 390 400
PLHFDENSFF AGDKNEAHKL KEDFRLHFRN ISRIMDCVGC FKCRLWGKLQ
410 420 430 440 450
TQGLGTALKI LFSEKLIANM PESGPSYEFQ LTRQEIVSLF NAFGRISTSV
460
RELENFRHLL QNVH
Length:464
Mass (Da):54,084
Last modified:March 24, 2009 - v2
Checksum:iFED19B00E1FBD2A1
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2I3BPM1A0A2I3BPM1_MOUSE
ERO1-like (S. cerevisiae)
Ero1l
229Annotation score:
A0A2I3BRB3A0A2I3BRB3_MOUSE
ERO1-like (S. cerevisiae)
Ero1l
64Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130C → G in AAH25102 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF144695 mRNA Translation: AAF20364.1
BC025102 mRNA Translation: AAH25102.1
AK009667 mRNA Translation: BAB26428.1
CCDSiCCDS36893.1
RefSeqiNP_056589.1, NM_015774.3
UniGeneiMm.387108

Genome annotation databases

EnsembliENSMUST00000022378; ENSMUSP00000022378; ENSMUSG00000021831
GeneIDi50527
KEGGimmu:50527
UCSCiuc007tgm.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF144695 mRNA Translation: AAF20364.1
BC025102 mRNA Translation: AAH25102.1
AK009667 mRNA Translation: BAB26428.1
CCDSiCCDS36893.1
RefSeqiNP_056589.1, NM_015774.3
UniGeneiMm.387108

3D structure databases

ProteinModelPortaliQ8R180
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R180, 1 interactor
MINTiQ8R180
STRINGi10090.ENSMUSP00000022378

PTM databases

iPTMnetiQ8R180
PhosphoSitePlusiQ8R180
SwissPalmiQ8R180

Proteomic databases

EPDiQ8R180
MaxQBiQ8R180
PaxDbiQ8R180
PRIDEiQ8R180

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022378; ENSMUSP00000022378; ENSMUSG00000021831
GeneIDi50527
KEGGimmu:50527
UCSCiuc007tgm.2 mouse

Organism-specific databases

CTDi50527
MGIiMGI:1354385 Ero1l

Phylogenomic databases

eggNOGiKOG2608 Eukaryota
COG5061 LUCA
GeneTreeiENSGT00390000007753
HOGENOMiHOG000012778
HOVERGENiHBG051507
InParanoidiQ8R180
KOiK10950
OMAiMDCVGCL
OrthoDBiEOG091G04ZQ
PhylomeDBiQ8R180
TreeFamiTF314471

Enzyme and pathway databases

ReactomeiR-MMU-264876 Insulin processing
R-MMU-3299685 Detoxification of Reactive Oxygen Species

Miscellaneous databases

ChiTaRSiEro1l mouse
PROiPR:Q8R180
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021831 Expressed in 285 organ(s), highest expression level in multi-cellular organism
CleanExiMM_ERO1L
ExpressionAtlasiQ8R180 baseline and differential
GenevisibleiQ8R180 MM

Family and domain databases

InterProiView protein in InterPro
IPR007266 Ero1
IPR037192 ERO1-like_sf
PANTHERiPTHR12613 PTHR12613, 1 hit
PfamiView protein in Pfam
PF04137 ERO1, 1 hit
PIRSFiPIRSF017205 ERO1, 1 hit
SUPFAMiSSF110019 SSF110019, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiERO1A_MOUSE
AccessioniPrimary (citable) accession number: Q8R180
Secondary accession number(s): Q9CV47, Q9QY03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 24, 2009
Last modified: November 7, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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