UniProtKB - Q8R173 (ZDHC3_MOUSE)
Protein
Palmitoyltransferase ZDHHC3
Gene
Zdhhc3
Organism
Mus musculus (Mouse)
Status
Functioni
Golgi-localized palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates (PubMed:15229235, PubMed:15603741, PubMed:16129400, PubMed:19001095, PubMed:23793055, PubMed:25253725, PubMed:26487721). Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA (Probable). Plays an important role in G protein-coupled receptor signaling pathways involving GNAQ and potentially other heterotrimeric G proteins by regulating their dynamic association with the plasma membrane (PubMed:19001095). Palmitoylates ITGA6 and ITGB4, thereby regulating the alpha-6/beta-4 integrin localization, expression and function in cell adhesion to laminin (By similarity). Plays a role in the TRAIL-activated apoptotic signaling pathway most probably through the palmitoylation and localization to the plasma membrane of TNFRSF10A (By similarity). In the brain, by palmitoylating the gamma subunit GABRG2 of GABA(A) receptors and regulating their postsynaptic accumulation, plays a role in synaptic GABAergic inhibitory function and GABAergic innervation (PubMed:15229235, PubMed:17151279, PubMed:27875292). Palmitoylates the neuronal protein GAP43 which is also involved in the formation of GABAergic synapses (PubMed:27875292). Palmitoylates NCDN thereby regulating its association with endosome membranes (PubMed:23687301). Probably palmitoylates PRCD and is involved in its proper localization within the photoreceptor (PubMed:27613864). Could mediate the palmitoylation of NCAM1 and regulate neurite outgrowth (PubMed:27247265). Could palmitoylate DNAJC5 and regulate its localization to Golgi membranes (PubMed:18596047). Also constitutively palmitoylates DLG4 (PubMed:15603741). May also palmitoylate SNAP25 (PubMed:25253725). Could palmitoylate the glutamate receptors GRIA1 and GRIA2 but this has not been confirmed in vivo (PubMed:16129400, PubMed:27875292). Could also palmitoylate the D2 dopamine receptor DRD2 (By similarity).By similarity1 Publication13 Publications
May also function as a calcium transporter.1 Publication
Catalytic activityi
- EC:2.3.1.2255 PublicationsThis reaction proceeds in the forward1 Publication direction.
- L-cysteinyl-[protein] + tetradecanoyl-CoA = CoA + S-tetradecanoyl-L-cysteinyl-[protein]1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 157 | S-palmitoyl cysteine intermediatePROSITE-ProRule annotation4 Publications | 1 |
GO - Molecular functioni
- palmitoyltransferase activity Source: MGI
- protein-cysteine S-myristoyltransferase activity Source: UniProtKB
- protein-cysteine S-palmitoyltransferase activity Source: UniProtKB
- protein-cysteine S-stearoyltransferase activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- lipoprotein localization to membrane Source: UniProtKB
- peptidyl-L-cysteine S-palmitoylation Source: UniProtKB
- positive regulation of receptor localization to synapse Source: UniProtKB
- positive regulation of synaptic transmission, GABAergic Source: UniProtKB
- protein localization to plasma membrane Source: UniProtKB
- protein palmitoylation Source: UniProtKB
- protein targeting Source: MGI
- protein targeting to membrane Source: GO_Central
- regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
- TRAIL-activated apoptotic signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.3.1.225, 3474 |
Protein family/group databases
TCDBi | 8.A.114.1.3, the huntington-interacting protein 14 (hip14) family |
Chemistry databases
SwissLipidsi | SLP:000001950 |
Names & Taxonomyi
Protein namesi | Recommended name: Palmitoyltransferase ZDHHC3Curated (EC:2.3.1.2255 Publications)Alternative name(s): Acyltransferase ZDHHC31 Publication (EC:2.3.1.-1 Publication) GABA-A receptor-associated membrane protein 1Imported Golgi-specific DHHC zinc finger protein1 Publication Zinc finger DHHC domain-containing protein 3Imported Short name: DHHC-31 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1926134, Zdhhc3 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 4 Publications; Multi-pass membrane protein Sequence analysis
Note: Localizes to the Golgi cis cisterna.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 47 | CytoplasmicSequence analysisAdd BLAST | 47 | |
Transmembranei | 48 – 68 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 69 – 72 | LumenalSequence analysis | 4 | |
Transmembranei | 73 – 93 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 94 – 171 | CytoplasmicSequence analysisAdd BLAST | 78 | |
Transmembranei | 172 – 192 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 193 – 214 | LumenalSequence analysisAdd BLAST | 22 | |
Transmembranei | 215 – 235 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 236 – 299 | CytoplasmicSequence analysisAdd BLAST | 64 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Homozygous knockout mice are viable, fertile and do not display overt phenotype (PubMed:27875292). Male show a modest reduction in body weight (PubMed:27875292). Zdhhc3 and Zdhhc7 double knockout mice show a perinatally lethal phenotype (PubMed:27875292).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 18 | Y → F: Decreased phosphorylation by FGFR1. 1 Publication | 1 | |
Mutagenesisi | 61 | V → R: Loss of function in calcium transport. 1 Publication | 1 | |
Mutagenesisi | 129 | C → S: No effect on ZDHHC3 palmitoylation. Changed protein structure. Decreased protein-cysteine S-palmitoyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 132 | C → S: No effect on ZDHHC3 palmitoylation. Changed protein structure. Decreased protein-cysteine S-palmitoyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 133 | C → S: No effect on ZDHHC3 palmitoylation. No effect on protein-cysteine S-palmitoyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 146 | C → S: Decreased ZDHHC3 palmitoylation. Changed protein structure. Decreased protein-cysteine S-palmitoyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 157 | C → S: Loss of protein-cysteine S-acyltransferase activity. Loss of ZDHHC3 palmitoylation. 4 Publications | 1 | |
Mutagenesisi | 182 – 184 | ISL → SSV: Increased protein-cysteine S-stearoyltransferase activity. 1 Publication | 3 | |
Mutagenesisi | 295 | Y → F: Decreased phosphorylation by SRC and increased autopalmitoylation; when associated with F-297. 1 Publication | 1 | |
Mutagenesisi | 297 | Y → F: Decreased phosphorylation by SRC and increased autopalmitoylation; when associated with F-295. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212863 | 1 – 299 | Palmitoyltransferase ZDHHC3Add BLAST | 299 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 18 | Phosphotyrosine; by FGFR11 Publication | 1 | |
Lipidationi | 146 | S-palmitoyl cysteine1 Publication | 1 |
Post-translational modificationi
Phosphorylation by FGFR1 and SRC probably regulates the palmitoyltransferase activity.1 Publication
Autopalmitoylated.4 Publications
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | Q8R173 |
MaxQBi | Q8R173 |
PaxDbi | Q8R173 |
PeptideAtlasi | Q8R173 |
PRIDEi | Q8R173 |
PTM databases
iPTMneti | Q8R173 |
PhosphoSitePlusi | Q8R173 |
SwissPalmi | Q8R173 |
Expressioni
Tissue specificityi
Widely expressed, with highest levels in brain. In the brain, expressed only in neurons (at protein level).3 Publications
Gene expression databases
Bgeei | ENSMUSG00000025786, Expressed in granulocyte and 301 other tissues |
ExpressionAtlasi | Q8R173, baseline and differential |
Genevisiblei | Q8R173, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
IntActi | Q8R173, 1 interactor |
MINTi | Q8R173 |
STRINGi | 10090.ENSMUSP00000117392 |
Miscellaneous databases
RNActi | Q8R173, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 126 – 177 | DHHCPROSITE-ProRule annotationAdd BLAST | 52 |
Domaini
The DHHC domain is required for palmitoyltransferase activity.By similarity
Sequence similaritiesi
Belongs to the DHHC palmitoyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000155721 |
HOGENOMi | CLU_048061_1_1_1 |
InParanoidi | Q8R173 |
OMAi | RAWCVKD |
OrthoDBi | 1491968at2759 |
PhylomeDBi | Q8R173 |
TreeFami | TF319798 |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
Q8R173-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMLIPTHHFR DIERKPEYLQ PEKCAPPPFP GPAGAMWFIR DGCGIACAIV
60 70 80 90 100
TWFLVLYAEF VVLFVMLVPS RDYAYSIING IVFNLLAFLA LASHCRAMLT
110 120 130 140 150
DPGAVPKGNA TKEFIESLQL KPGQVVYKCP KCCSIKPDRA HHCSVCKRCI
160 170 180 190 200
RKMDHHCPWV NNCVGENNQK YFVLFTMYIA LISLHALIMV GFHFLHCFEE
210 220 230 240 250
DWTKCSSFSP PTTVILLILL CFEALLFLIF TSVMFGTQVH SICTDETGIE
260 270 280 290
QLKKEERRWA KKTKWMNMKA VFGHPFSLGW ASPFATPDQG KADPYQYVV
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A571BEV7 | A0A571BEV7_MOUSE | Palmitoyltransferase | Zdhhc3 | 332 | Annotation score: | ||
D3Z2A6 | D3Z2A6_MOUSE | Palmitoyltransferase ZDHHC3 | Zdhhc3 | 83 | Annotation score: | ||
D6RFV6 | D6RFV6_MOUSE | Palmitoyltransferase ZDHHC3 | Zdhhc3 | 112 | Annotation score: | ||
Q05D46 | Q05D46_MOUSE | Palmitoyltransferase ZDHHC3 | Zdhhc3 | 104 | Annotation score: | ||
D6RDS4 | D6RDS4_MOUSE | Palmitoyltransferase ZDHHC3 | Zdhhc3 | 122 | Annotation score: | ||
D6RHA3 | D6RHA3_MOUSE | Palmitoyltransferase ZDHHC3 | Zdhhc3 | 128 | Annotation score: | ||
F6Q2M4 | F6Q2M4_MOUSE | Palmitoyltransferase ZDHHC3 | Zdhhc3 | 65 | Annotation score: | ||
D6RHS2 | D6RHS2_MOUSE | Palmitoyltransferase ZDHHC3 | Zdhhc3 | 154 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 246 | E → G in BAC37939 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB088044 mRNA Translation: BAC02941.1 AY166672 mRNA Translation: AAO27359.1 AK080541 mRNA Translation: BAC37939.1 BC025126 mRNA Translation: AAH25126.1 BC052464 mRNA Translation: AAH52464.1 |
CCDSi | CCDS23654.1 |
RefSeqi | NP_081193.2, NM_026917.5 |
Genome annotation databases
Ensembli | ENSMUST00000147563; ENSMUSP00000117392; ENSMUSG00000025786 |
GeneIDi | 69035 |
KEGGi | mmu:69035 |
UCSCi | uc009sfq.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB088044 mRNA Translation: BAC02941.1 AY166672 mRNA Translation: AAO27359.1 AK080541 mRNA Translation: BAC37939.1 BC025126 mRNA Translation: AAH25126.1 BC052464 mRNA Translation: AAH52464.1 |
CCDSi | CCDS23654.1 |
RefSeqi | NP_081193.2, NM_026917.5 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q8R173, 1 interactor |
MINTi | Q8R173 |
STRINGi | 10090.ENSMUSP00000117392 |
Chemistry databases
SwissLipidsi | SLP:000001950 |
Protein family/group databases
TCDBi | 8.A.114.1.3, the huntington-interacting protein 14 (hip14) family |
PTM databases
iPTMneti | Q8R173 |
PhosphoSitePlusi | Q8R173 |
SwissPalmi | Q8R173 |
Proteomic databases
EPDi | Q8R173 |
MaxQBi | Q8R173 |
PaxDbi | Q8R173 |
PeptideAtlasi | Q8R173 |
PRIDEi | Q8R173 |
Protocols and materials databases
Antibodypediai | 3251, 135 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000147563; ENSMUSP00000117392; ENSMUSG00000025786 |
GeneIDi | 69035 |
KEGGi | mmu:69035 |
UCSCi | uc009sfq.2, mouse |
Organism-specific databases
CTDi | 51304 |
MGIi | MGI:1926134, Zdhhc3 |
Phylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000155721 |
HOGENOMi | CLU_048061_1_1_1 |
InParanoidi | Q8R173 |
OMAi | RAWCVKD |
OrthoDBi | 1491968at2759 |
PhylomeDBi | Q8R173 |
TreeFami | TF319798 |
Enzyme and pathway databases
BRENDAi | 2.3.1.225, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 69035, 1 hit in 17 CRISPR screens |
ChiTaRSi | Zdhhc3, mouse |
PROi | PR:Q8R173 |
RNActi | Q8R173, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025786, Expressed in granulocyte and 301 other tissues |
ExpressionAtlasi | Q8R173, baseline and differential |
Genevisiblei | Q8R173, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZDHC3_MOUSE | |
Accessioni | Q8R173Primary (citable) accession number: Q8R173 Secondary accession number(s): Q6EMK3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2003 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families