UniProtKB - Q8R138 (TM119_MOUSE)
Protein
Transmembrane protein 119
Gene
Tmem119
Organism
Mus musculus (Mouse)
Status
Functioni
Plays an important role in bone formation and normal bone mineralization (PubMed:26207632, PubMed:22416756, PubMed:20025746). Promotes the differentiation of myoblasts into osteoblasts (PubMed:22416756, PubMed:20025746, PubMed:22579779). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway (PubMed:21239498, PubMed:22579779). Upregulates the expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Essential for normal spermatogenesis and late testicular differentiation (PubMed:26207632).6 Publications
GO - Biological processi
- biomineral tissue development Source: UniProtKB-KW
- endochondral ossification Source: ARUK-UCL
- negative regulation of bone resorption Source: ARUK-UCL
- negative regulation of myotube differentiation Source: ARUK-UCL
- osteoblast differentiation Source: MGI
- positive regulation of bone development Source: UniProtKB
- positive regulation of bone mineralization Source: UniProtKB
- positive regulation of gene expression Source: ARUK-UCL
- positive regulation of osteoblast differentiation Source: ARUK-UCL
- positive regulation of osteoblast proliferation Source: UniProtKB
- positive regulation of protein phosphorylation Source: ARUK-UCL
- spermatid differentiation Source: UniProtKB
- spermatogenesis Source: UniProtKB
Keywordsi
Biological process | Biomineralization, Differentiation, Osteogenesis, Spermatogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Transmembrane protein 119Alternative name(s): Osteoblast induction factor Short name: OBIF |
Gene namesi | Name:Tmem119 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2385228, Tmem119 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein Sequence analysis
Other locations
- Cytoplasm 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 91 | ExtracellularSequence analysisAdd BLAST | 71 | |
Transmembranei | 92 – 112 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 113 – 280 | CytoplasmicSequence analysisAdd BLAST | 168 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Mice show a significant decrease in bone formation and bone mineralization and the mineralization defect is independent of calcium and phosphate metabolisms. Testis is smaller, sperm number is significantly decreased and testicular differentiation is perturbed (PubMed:26207632). Significantly reduced cortical thickness in the mid-shaft of the femur at postnatal day 14 (P14), and progressive bone hypoplasia after 8 weeks (PubMed:22416756).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 36 | S → A: Loss of glycosylation. No effect on osteoblast mineralization. Loss of glycosylation; when associated with A-43; A-54; A-60 AND A-67. 1 Publication | 1 | |
Mutagenesisi | 43 | S → A: No loss of glycosylation. Loss of glycosylation; when associated with A-36; A-54; A-60 AND A-67. 1 Publication | 1 | |
Mutagenesisi | 54 | T → A: No loss of glycosylation. Loss of glycosylation; when associated with A-36; A-43; A-60 AND A-67. 1 Publication | 1 | |
Mutagenesisi | 60 | T → A: No loss of glycosylation. Loss of glycosylation; when associated with A-36; A-43; A-54 AND A-67. 1 Publication | 1 | |
Mutagenesisi | 67 | T → A: No loss of glycosylation. Loss of glycosylation; when associated with A-36; A-43; A-54 AND A-60. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000251221 | 21 – 280 | Transmembrane protein 119Add BLAST | 260 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 36 | O-linked (GalNAc...) serine1 Publication | 1 | |
Modified residuei | 269 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q8R138 |
MaxQBi | Q8R138 |
PaxDbi | Q8R138 |
PeptideAtlasi | Q8R138 |
PRIDEi | Q8R138 |
PTM databases
GlyGeni | Q8R138, 1 site |
iPTMneti | Q8R138 |
PhosphoSitePlusi | Q8R138 |
Expressioni
Tissue specificityi
Expressed in spermatocytes and spermatids in the developing testis (at protein level). Expressed in the brain, heart, lung, spleen, skeletal muscle, ovary, testis and epididymis (PubMed:26207632). Predominantly expressed in osteoblasts (PubMed:20025746).2 Publications
Developmental stagei
Highly expressed in early and late stage osteoblasts of developing embryos (at protein level).1 Publication
Inductioni
By parathyroid hormone (PTH) in osteoblasts (at protein level).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000054675, Expressed in vault of skull and 220 other tissues |
Genevisiblei | Q8R138, MM |
Interactioni
Subunit structurei
Interacts with SMAD1, SMAD5 and RUNX2.
1 PublicationProtein-protein interaction databases
IntActi | Q8R138, 2 interactors |
MINTi | Q8R138 |
STRINGi | 10090.ENSMUSP00000070551 |
Miscellaneous databases
RNActi | Q8R138, protein |
Family & Domainsi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S283, Eukaryota |
GeneTreei | ENSGT00390000017134 |
HOGENOMi | CLU_086693_0_0_1 |
InParanoidi | Q8R138 |
OMAi | EEPCSGV |
OrthoDBi | 1284233at2759 |
PhylomeDBi | Q8R138 |
TreeFami | TF336958 |
Family and domain databases
InterProi | View protein in InterPro IPR031453, TMEM119 |
PANTHERi | PTHR28645, PTHR28645, 1 hit |
Pfami | View protein in Pfam PF15724, TMEM119, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8R138-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVPWFLLSLL LLARPVPGVA YSVSLPASFL EDVAGSGEAE GSSASSPSLP
60 70 80 90 100
PPGTPAFSPT PERPQPTALD GPVPPTNLLE GIMDFFRQYV MLIAVVGSLT
110 120 130 140 150
FLIMFIVCAA LITRQKHKAT AYYPSSFPEK KYVDQRDRAG GPRTFSEVPD
160 170 180 190 200
RAPDSRHEEG LDTSHQLQAD ILAATQNLRS PARALPGNGE GAKPVKGGSE
210 220 230 240 250
EEEEEVLSGQ EEAQEAPVCG VTEEKLGVPE ESVSAEAEGV PATSEGQGEA
260 270 280
EGSFSLAQES QGATGPPESP CACNRVSPSV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE0CYG1 | E0CYG1_MOUSE | Transmembrane protein 119 | Tmem119 | 33 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 78 | L → H in BAC37292 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK078473 mRNA Translation: BAC37292.1 AK170712 mRNA Translation: BAE41970.1 BC025600 mRNA Translation: AAH25600.1 |
CCDSi | CCDS19554.1 |
RefSeqi | NP_666274.1, NM_146162.2 XP_006530350.1, XM_006530287.3 |
Genome annotation databases
Ensembli | ENSMUST00000067853; ENSMUSP00000070551; ENSMUSG00000054675 |
GeneIDi | 231633 |
KEGGi | mmu:231633 |
UCSCi | uc008yyq.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK078473 mRNA Translation: BAC37292.1 AK170712 mRNA Translation: BAE41970.1 BC025600 mRNA Translation: AAH25600.1 |
CCDSi | CCDS19554.1 |
RefSeqi | NP_666274.1, NM_146162.2 XP_006530350.1, XM_006530287.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q8R138, 2 interactors |
MINTi | Q8R138 |
STRINGi | 10090.ENSMUSP00000070551 |
PTM databases
GlyGeni | Q8R138, 1 site |
iPTMneti | Q8R138 |
PhosphoSitePlusi | Q8R138 |
Proteomic databases
jPOSTi | Q8R138 |
MaxQBi | Q8R138 |
PaxDbi | Q8R138 |
PeptideAtlasi | Q8R138 |
PRIDEi | Q8R138 |
Protocols and materials databases
Antibodypediai | 55081, 19 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000067853; ENSMUSP00000070551; ENSMUSG00000054675 |
GeneIDi | 231633 |
KEGGi | mmu:231633 |
UCSCi | uc008yyq.1, mouse |
Organism-specific databases
CTDi | 338773 |
MGIi | MGI:2385228, Tmem119 |
Phylogenomic databases
eggNOGi | ENOG502S283, Eukaryota |
GeneTreei | ENSGT00390000017134 |
HOGENOMi | CLU_086693_0_0_1 |
InParanoidi | Q8R138 |
OMAi | EEPCSGV |
OrthoDBi | 1284233at2759 |
PhylomeDBi | Q8R138 |
TreeFami | TF336958 |
Miscellaneous databases
BioGRID-ORCSi | 231633, 3 hits in 17 CRISPR screens |
ChiTaRSi | Tmem119, mouse |
PROi | PR:Q8R138 |
RNActi | Q8R138, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000054675, Expressed in vault of skull and 220 other tissues |
Genevisiblei | Q8R138, MM |
Family and domain databases
InterProi | View protein in InterPro IPR031453, TMEM119 |
PANTHERi | PTHR28645, PTHR28645, 1 hit |
Pfami | View protein in Pfam PF15724, TMEM119, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TM119_MOUSE | |
Accessioni | Q8R138Primary (citable) accession number: Q8R138 Secondary accession number(s): Q8BP14 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2006 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot