UniProtKB - Q8R0Z5 (MFRN2_MOUSE)
Mitoferrin-2
Slc25a28
Functioni
Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe2+, is then probably delivered to ferrochelatase to catalyze Fe2+ incorporation into protoprophyrin IX to make heme (Probable).
2 PublicationsGO - Molecular functioni
- ferrous iron transmembrane transporter activity Source: Reactome
- iron ion transmembrane transporter activity Source: GO_Central
GO - Biological processi
- iron import into the mitochondrion Source: GO_Central
- iron ion homeostasis Source: UniProtKB-KW
Keywordsi
Biological process | Ion transport, Iron transport, Transport |
Ligand | Iron |
Protein family/group databases
TCDBi | 2.A.29.5.4, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Mitoferrin-2Alternative name(s): Mitochondrial RNA-splicing protein 3/4 homolog Short name: MRS3/4 Mitochondrial iron transporter 2 Solute carrier family 25 member 28 |
Gene namesi | Name:Slc25a28 Synonyms:Mfrn2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2180509, Slc25a28 |
VEuPathDBi | HostDB:ENSMUSG00000040414 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Isoform 1 and isoform 2 are both localized in the mitochondrion.
Mitochondrion
- mitochondrial inner membrane Source: Reactome
- mitochondrion Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 72 – 91 | Helical; Name=1Sequence analysisAdd BLAST | 20 | |
Transmembranei | 133 – 152 | Helical; Name=2Sequence analysisAdd BLAST | 20 | |
Transmembranei | 170 – 189 | Helical; Name=3Sequence analysisAdd BLAST | 20 | |
Transmembranei | 227 – 246 | Helical; Name=4Sequence analysisAdd BLAST | 20 | |
Transmembranei | 261 – 280 | Helical; Name=5Sequence analysisAdd BLAST | 20 | |
Transmembranei | 327 – 346 | Helical; Name=6Sequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000235256 | 1 – 364 | Mitoferrin-2Add BLAST | 364 |
Proteomic databases
EPDi | Q8R0Z5 |
MaxQBi | Q8R0Z5 |
PaxDbi | Q8R0Z5 |
PeptideAtlasi | Q8R0Z5 |
PRIDEi | Q8R0Z5 |
ProteomicsDBi | 295598 [Q8R0Z5-1] 295599 [Q8R0Z5-2] |
PTM databases
PhosphoSitePlusi | Q8R0Z5 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000040414, Expressed in epididymal fat pad and 310 other tissues |
ExpressionAtlasi | Q8R0Z5, baseline and differential |
Genevisiblei | Q8R0Z5, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000036913 |
Miscellaneous databases
RNActi | Q8R0Z5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 70 – 158 | Solcar 1Add BLAST | 89 | |
Repeati | 168 – 252 | Solcar 2Add BLAST | 85 | |
Repeati | 263 – 352 | Solcar 3Add BLAST | 90 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 28 | DisorderedSequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0760, Eukaryota |
GeneTreei | ENSGT00940000157049 |
HOGENOMi | CLU_015166_3_1_1 |
InParanoidi | Q8R0Z5 |
OMAi | KVMNPSQ |
OrthoDBi | 1056670at2759 |
PhylomeDBi | Q8R0Z5 |
TreeFami | TF314118 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELEGRSAGG VAGGPAAGPG RSPGESALLD GWLQRGVGRG AGGGEAGAYQ
60 70 80 90 100
PPVRLDPESG PEYEALPAGA TVTTHMVAGA VAGILEHCVM YPIDCVKTRM
110 120 130 140 150
QSLQPDPAAR YRNVLEALWR IMRTEGLWRP MRGLNVTATG AGPAHALYFA
160 170 180 190 200
CYEKLKKTLS DVIHPGGNSH IANGAAGCVA TLLHDAAMNP AEVVKQRMQM
210 220 230 240 250
YNSPYHRVTD CVRAVWQNEG AGAFYRSYTT QLTMNVPFQA IHFMTYEFLQ
260 270 280 290 300
EHFNPQRRYN PSSHVLCGAC AGAVAAAATT PLDVCKTLLN TQESLALNSN
310 320 330 340 350
ITGHITGMAS AFRTVYQVGG VTAYFRGVQA RVIYQIPSTA IAWSVYEFFK
360
YLITKRQEEW RAGK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A494BA64 | A0A494BA64_MOUSE | Mitoferrin-2 | Slc25a28 | 83 | Annotation score: | ||
A0A494BAC6 | A0A494BAC6_MOUSE | Mitoferrin-2 | Slc25a28 | 111 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 276 | A → D in AAH23172 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018416 | 1 – 187 | Missing in isoform 2. 2 PublicationsAdd BLAST | 187 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF377993 mRNA Translation: AAM46109.1 AF377994 mRNA Translation: AAM46110.1 BC023172 mRNA Translation: AAH23172.1 BC025908 mRNA Translation: AAH25908.1 |
CCDSi | CCDS29834.1 [Q8R0Z5-1] |
RefSeqi | NP_660138.1, NM_145156.1 [Q8R0Z5-1] XP_006527161.1, XM_006527098.3 |
Genome annotation databases
Ensembli | ENSMUST00000046038; ENSMUSP00000036913; ENSMUSG00000040414 [Q8R0Z5-1] |
GeneIDi | 246696 |
KEGGi | mmu:246696 |
UCSCi | uc008hom.2, mouse [Q8R0Z5-2] uc008hon.2, mouse [Q8R0Z5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF377993 mRNA Translation: AAM46109.1 AF377994 mRNA Translation: AAM46110.1 BC023172 mRNA Translation: AAH23172.1 BC025908 mRNA Translation: AAH25908.1 |
CCDSi | CCDS29834.1 [Q8R0Z5-1] |
RefSeqi | NP_660138.1, NM_145156.1 [Q8R0Z5-1] XP_006527161.1, XM_006527098.3 |
3D structure databases
AlphaFoldDBi | Q8R0Z5 |
SMRi | Q8R0Z5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000036913 |
Protein family/group databases
TCDBi | 2.A.29.5.4, the mitochondrial carrier (mc) family |
PTM databases
PhosphoSitePlusi | Q8R0Z5 |
Proteomic databases
EPDi | Q8R0Z5 |
MaxQBi | Q8R0Z5 |
PaxDbi | Q8R0Z5 |
PeptideAtlasi | Q8R0Z5 |
PRIDEi | Q8R0Z5 |
ProteomicsDBi | 295598 [Q8R0Z5-1] 295599 [Q8R0Z5-2] |
Protocols and materials databases
Antibodypediai | 31086, 139 antibodies from 19 providers |
DNASUi | 246696 |
Genome annotation databases
Ensembli | ENSMUST00000046038; ENSMUSP00000036913; ENSMUSG00000040414 [Q8R0Z5-1] |
GeneIDi | 246696 |
KEGGi | mmu:246696 |
UCSCi | uc008hom.2, mouse [Q8R0Z5-2] uc008hon.2, mouse [Q8R0Z5-1] |
Organism-specific databases
CTDi | 81894 |
MGIi | MGI:2180509, Slc25a28 |
VEuPathDBi | HostDB:ENSMUSG00000040414 |
Phylogenomic databases
eggNOGi | KOG0760, Eukaryota |
GeneTreei | ENSGT00940000157049 |
HOGENOMi | CLU_015166_3_1_1 |
InParanoidi | Q8R0Z5 |
OMAi | KVMNPSQ |
OrthoDBi | 1056670at2759 |
PhylomeDBi | Q8R0Z5 |
TreeFami | TF314118 |
Miscellaneous databases
BioGRID-ORCSi | 246696, 6 hits in 74 CRISPR screens |
ChiTaRSi | Slc25a28, mouse |
PROi | PR:Q8R0Z5 |
RNActi | Q8R0Z5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040414, Expressed in epididymal fat pad and 310 other tissues |
ExpressionAtlasi | Q8R0Z5, baseline and differential |
Genevisiblei | Q8R0Z5, MM |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | MFRN2_MOUSE | |
Accessioni | Q8R0Z5Primary (citable) accession number: Q8R0Z5 Secondary accession number(s): Q8K4J8, Q8K4J9, Q8R578 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | June 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families