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Protein

Sphingosine-1-phosphate lyase 1

Gene

Sgpl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis.1 Publication

Catalytic activityi

Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde.1 Publication

Cofactori

pyridoxal 5'-phosphateBy similarity

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

GO - Molecular functioni

  • carboxy-lyase activity Source: InterPro
  • pyridoxal phosphate binding Source: GO_Central
  • sphinganine-1-phosphate aldolase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processApoptosis, Lipid metabolism, Sphingolipid metabolism
LigandPyridoxal phosphate

Enzyme and pathway databases

BRENDAi4.1.2.27 3474
ReactomeiR-MMU-1660661 Sphingolipid de novo biosynthesis
UniPathwayi
UPA00222

Chemistry databases

SwissLipidsiSLP:000000935

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine-1-phosphate lyase 1Curated (EC:4.1.2.271 Publication)
Short name:
S1PL
Short name:
SP-lyase 1
Short name:
SPL 1
Short name:
mSPL
Alternative name(s):
Sphingosine-1-phosphate aldolase
Gene namesi
Name:Sgpl1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1261415 Sgpl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 40LumenalSequence analysisAdd BLAST40
Transmembranei41 – 61Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini62 – 568CytoplasmicSequence analysisAdd BLAST507

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice lacking Sgpl1 exhibit complete podocyte foot process effacement and absence of slit diaphragms in kidney (PubMed:9464245, PubMed:28165339). They display hypoalbuminemia and an elevated urinary albumin/creatinine ratio (PubMed:28165339). They also display abnormal adrenal gland morphology and defective expression of enzymes involved in steroidogenesis in this tissue (PubMed:28165343).3 Publications

Chemistry databases

ChEMBLiCHEMBL5009

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001470131 – 568Sphingosine-1-phosphate lyase 1Add BLAST568

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei353N6-(pyridoxal phosphate)lysine; alternateBy similarity1
Modified residuei353N6-acetyllysine; alternateBy similarity1
Modified residuei356Nitrated tyrosineBy similarity1
Modified residuei366Nitrated tyrosineBy similarity1
Modified residuei564PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Nitration, Phosphoprotein

Proteomic databases

EPDiQ8R0X7
MaxQBiQ8R0X7
PaxDbiQ8R0X7
PRIDEiQ8R0X7

PTM databases

iPTMnetiQ8R0X7
PhosphoSitePlusiQ8R0X7
SwissPalmiQ8R0X7

Expressioni

Tissue specificityi

Highest levels are found in liver, followed by kidney, lung, heart and brain.2 Publications

Gene expression databases

BgeeiENSMUSG00000020097 Expressed in 286 organ(s), highest expression level in olfactory epithelium
CleanExiMM_SGPL1
ExpressionAtlasiQ8R0X7 baseline and differential
GenevisibleiQ8R0X7 MM

Interactioni

Protein-protein interaction databases

BioGridi203199, 3 interactors
IntActiQ8R0X7, 1 interactor
MINTiQ8R0X7
STRINGi10090.ENSMUSP00000090155

Chemistry databases

BindingDBiQ8R0X7

Structurei

3D structure databases

ProteinModelPortaliQ8R0X7
SMRiQ8R0X7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1383 Eukaryota
COG0076 LUCA
GeneTreeiENSGT00390000000046
HOVERGENiHBG056982
InParanoidiQ8R0X7
KOiK01634
OrthoDBiEOG091G040L
TreeFamiTF300777

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR002129 PyrdxlP-dep_de-COase
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00282 Pyridoxal_deC, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q8R0X7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPGTDLLKLK DFEPYLEILE SYSTKAKNYV NGYCTKYEPW QLIAWSVLCT
60 70 80 90 100
LLIVWVYELI FQPESLWSRF KKKLFKLIRK MPFIGRKIEQ QVSKAKKDLV
110 120 130 140 150
KNMPFLKVDK DYVKTLPAQG MGTAEVLERL KEYSSMDGSW QEGKASGAVY
160 170 180 190 200
NGEPKLTELL VQAYGEFTWS NPLHPDIFPG LRKLEAEIVR MTCSLFNGGP
210 220 230 240 250
DSCGCVTSGG TESILMACKA YRDLALEKGI KTPEIVAPES AHAAFDKAAH
260 270 280 290 300
YFGMKIVRVA LKKNMEVDVQ AMKRAISRNT AMLVCSTPQF PHGVMDPVPE
310 320 330 340 350
VAKLAVRYKI PLHVDACLGG FLIVFMEKAG YPLEKPFDFR VKGVTSISAD
360 370 380 390 400
THKYGYAPKG SSVVMYSNEK YRTYQFFVGA DWQGGVYASP SIAGSRPGGI
410 420 430 440 450
IAACWAALMH FGENGYVEAT KQIIKTARFL KSELENIKNI FIFGDPQLSV
460 470 480 490 500
IALGSNDFDI YRLSNMMSAK GWNFNYLQFP RSIHFCITLV HTRKRVAIQF
510 520 530 540 550
LKDIRESVTQ IMKNPKAKTT GMGAIYGMAQ ATIDRKLVAE ISSVFLDCLY
560
TTDPVTQGNQ MNGSPKPR
Length:568
Mass (Da):63,677
Last modified:June 1, 2002 - v1
Checksum:iFA5D52E4E49DF09E
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6REF7D6REF7_MOUSE
Sphingosine-1-phosphate lyase 1
Sgpl1
487Annotation score:
D3YZT4D3YZT4_MOUSE
Sphingosine-1-phosphate lyase 1
Sgpl1
199Annotation score:
D3YY13D3YY13_MOUSE
Sphingosine-1-phosphate lyase 1
Sgpl1
58Annotation score:
D3Z1Z3D3Z1Z3_MOUSE
Sphingosine-1-phosphate lyase 1
Sgpl1
104Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti305A → T in AAC03768 (PubMed:9464245).Curated1
Sequence conflicti473N → K in BAC31437 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036894 mRNA Translation: AAC03768.1
AK036747 mRNA Translation: BAC29562.1
AK037789 mRNA Translation: BAC29872.1
AK043024 mRNA Translation: BAC31437.1
AK049342 mRNA Translation: BAC33695.1
BC026135 mRNA Translation: AAH26135.1
CCDSiCCDS35914.1
PIRiJC5923
RefSeqiNP_001303602.1, NM_001316673.1
NP_001303603.1, NM_001316674.1
NP_033189.2, NM_009163.4
XP_017169349.1, XM_017313860.1
UniGeneiMm.412319
Mm.431862

Genome annotation databases

EnsembliENSMUST00000092498; ENSMUSP00000090155; ENSMUSG00000020097
ENSMUST00000122259; ENSMUSP00000112975; ENSMUSG00000020097
GeneIDi20397
KEGGimmu:20397
UCSCiuc007ffk.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036894 mRNA Translation: AAC03768.1
AK036747 mRNA Translation: BAC29562.1
AK037789 mRNA Translation: BAC29872.1
AK043024 mRNA Translation: BAC31437.1
AK049342 mRNA Translation: BAC33695.1
BC026135 mRNA Translation: AAH26135.1
CCDSiCCDS35914.1
PIRiJC5923
RefSeqiNP_001303602.1, NM_001316673.1
NP_001303603.1, NM_001316674.1
NP_033189.2, NM_009163.4
XP_017169349.1, XM_017313860.1
UniGeneiMm.412319
Mm.431862

3D structure databases

ProteinModelPortaliQ8R0X7
SMRiQ8R0X7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203199, 3 interactors
IntActiQ8R0X7, 1 interactor
MINTiQ8R0X7
STRINGi10090.ENSMUSP00000090155

Chemistry databases

BindingDBiQ8R0X7
ChEMBLiCHEMBL5009
SwissLipidsiSLP:000000935

PTM databases

iPTMnetiQ8R0X7
PhosphoSitePlusiQ8R0X7
SwissPalmiQ8R0X7

Proteomic databases

EPDiQ8R0X7
MaxQBiQ8R0X7
PaxDbiQ8R0X7
PRIDEiQ8R0X7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092498; ENSMUSP00000090155; ENSMUSG00000020097
ENSMUST00000122259; ENSMUSP00000112975; ENSMUSG00000020097
GeneIDi20397
KEGGimmu:20397
UCSCiuc007ffk.2 mouse

Organism-specific databases

CTDi8879
MGIiMGI:1261415 Sgpl1

Phylogenomic databases

eggNOGiKOG1383 Eukaryota
COG0076 LUCA
GeneTreeiENSGT00390000000046
HOVERGENiHBG056982
InParanoidiQ8R0X7
KOiK01634
OrthoDBiEOG091G040L
TreeFamiTF300777

Enzyme and pathway databases

UniPathwayi
UPA00222

BRENDAi4.1.2.27 3474
ReactomeiR-MMU-1660661 Sphingolipid de novo biosynthesis

Miscellaneous databases

ChiTaRSiSgpl1 mouse
PROiPR:Q8R0X7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020097 Expressed in 286 organ(s), highest expression level in olfactory epithelium
CleanExiMM_SGPL1
ExpressionAtlasiQ8R0X7 baseline and differential
GenevisibleiQ8R0X7 MM

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR002129 PyrdxlP-dep_de-COase
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00282 Pyridoxal_deC, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSGPL1_MOUSE
AccessioniPrimary (citable) accession number: Q8R0X7
Secondary accession number(s): O54955, Q8C942
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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