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Protein

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1

Gene

Wfikkn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei126 – 127Reactive bondPROSITE-ProRule annotation2

GO - Molecular functioni

GO - Biological processi

  • muscle fiber development Source: MGI
  • negative regulation of DNA binding Source: MGI
  • negative regulation of protein binding Source: MGI
  • negative regulation of proteolysis Source: MGI
  • negative regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • roof of mouth development Source: MGI
  • skeletal system development Source: MGI

Keywordsi

Molecular functionMetalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.953

Names & Taxonomyi

Protein namesi
Recommended name:
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1
Alternative name(s):
Growth and differentiation factor-associated serum protein 2
Short name:
GASP-2
Short name:
mGASP-2
Gene namesi
Name:Wfikkn1
Synonyms:Gasp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2670967 Wfikkn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000030781726 – 552WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1Add BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 69By similarity
Disulfide bondi52 ↔ 73By similarity
Disulfide bondi56 ↔ 68By similarity
Disulfide bondi62 ↔ 78By similarity
Disulfide bondi120 ↔ 150By similarity
Disulfide bondi124 ↔ 143By similarity
Disulfide bondi132 ↔ 161By similarity
Disulfide bondi211 ↔ 267By similarity
Disulfide bondi303 ↔ 355By similarity
Disulfide bondi310 ↔ 338By similarity
Disulfide bondi330 ↔ 351By similarity
Disulfide bondi363 ↔ 413By similarity
Disulfide bondi372 ↔ 396By similarity
Disulfide bondi388 ↔ 409By similarity
Disulfide bondi421 ↔ 493By similarity
Disulfide bondi424 ↔ 495By similarity
Disulfide bondi435 ↔ 544By similarity
Glycosylationi497N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8R0S6
PRIDEiQ8R0S6

PTM databases

PhosphoSitePlusiQ8R0S6

Expressioni

Gene expression databases

BgeeiENSMUSG00000071192 Expressed in 83 organ(s), highest expression level in ear vesicle
CleanExiMM_WFIKKN1
ExpressionAtlasiQ8R0S6 baseline and differential
GenevisibleiQ8R0S6 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093141

Structurei

3D structure databases

ProteinModelPortaliQ8R0S6
SMRiQ8R0S6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 82WAPPROSITE-ProRule annotationAdd BLAST54
Domaini112 – 163Kazal-likePROSITE-ProRule annotationAdd BLAST52
Domaini190 – 283Ig-like C2-typeAdd BLAST94
Domaini289 – 355BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd BLAST67
Domaini363 – 413BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd BLAST51
Domaini413 – 544NTRPROSITE-ProRule annotationAdd BLAST132

Domaini

The second BPTI/Kunitz inhibitor domain is able to inhibit trypsin. It has however no activity toward chymotrypsin, elastase, plasmin, pancreatic kallikrein, lung tryptase, plasma kallikrein, thrombin, urokinase or tissue plasminogen activator (By similarity).By similarity

Sequence similaritiesi

Belongs to the WFIKKN family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG4597 Eukaryota
ENOG41102GD LUCA
GeneTreeiENSGT00740000114929
HOGENOMiHOG000185176
HOVERGENiHBG108676
InParanoidiQ8R0S6
OMAiWEPRWAY
OrthoDBiEOG091G09P2
PhylomeDBiQ8R0S6
TreeFamiTF315349

Family and domain databases

CDDicd00109 KU, 1 hit
Gene3Di2.60.40.10, 1 hit
4.10.410.10, 2 hits
4.10.75.10, 1 hit
InterProiView protein in InterPro
IPR036645 Elafin-like_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR001134 Netrin_domain
IPR020901 Prtase_inh_Kunz-CS
IPR008993 TIMP-like_OB-fold
IPR008197 WAP_dom
IPR033638 WFIKKN1
PANTHERiPTHR10083:SF212 PTHR10083:SF212, 1 hit
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 2 hits
PF00095 WAP, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00408 IGc2, 1 hit
SM00131 KU, 2 hits
SM00217 WAP, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
SSF48726 SSF48726, 1 hit
SSF50242 SSF50242, 1 hit
SSF57256 SSF57256, 1 hit
SSF57362 SSF57362, 2 hits
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 1 hit
PS50279 BPTI_KUNITZ_2, 2 hits
PS50835 IG_LIKE, 1 hit
PS51465 KAZAL_2, 1 hit
PS50189 NTR, 1 hit
PS51390 WAP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8R0S6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPAPQPFLPL LFVFVLIHLT SETNLLPDPG SHPGMCPNEL SPHLWVDAQS
60 70 80 90 100
TCERECTGDQ DCAASEKCCT NVCGLQSCVA ARFPSGGPAV PETAASCEGF
110 120 130 140 150
QCPQQGSDCD IWDGQPVCRC RDRCEKEPSF TCASDGLTYY NRCYMDAEAC
160 170 180 190 200
LRGLHLHVVP CKHILSWPPS SPGPPETTAR PTPGAAPMPP ALYNSPSPQA
210 220 230 240 250
VHVGGTASLH CDVSGRPPPA VTWEKQSHQR ENLIMRPDQM YGNVVVTSIG
260 270 280 290 300
QLVLYNAQLE DAGLYTCTAR NAAGLLRADF PLSVLQRATT QDRDPGIPAL
310 320 330 340 350
AECQADTQAC VGPPTPHHVL WRFDPQRGSC MTFPALRCDG AARGFETYEA
360 370 380 390 400
CQQACVRGPG DVCALPAVQG PCQGWEPRWA YSPLLQQCHP FVYSGCEGNS
410 420 430 440 450
NNFETRESCE DACPVPRTPP CRACRLKSKL ALSLCRSDFA IVGRLTEVLE
460 470 480 490 500
EPEAAGGIAR VALDDVLKDD KMGLKFLGTK YLEVTLSGMD WACPCPNVTA
510 520 530 540 550
VDGPLVIMGE VREGVAVLDA NSYVRAASEK RVKKIVELLE KKACELLNRF

QD
Length:552
Mass (Da):59,794
Last modified:October 23, 2007 - v2
Checksum:iBD7A876E4ED19B4A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BJQ6H3BJQ6_MOUSE
WAP, Kazal, immunoglobulin, Kunitz ...
Wfikkn1
147Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC159277 Genomic DNA No translation available.
BC026460 mRNA Translation: AAH26460.1
CCDSiCCDS50038.1
RefSeqiNP_001093924.1, NM_001100454.1
UniGeneiMm.328871

Genome annotation databases

EnsembliENSMUST00000095487; ENSMUSP00000093141; ENSMUSG00000071192
GeneIDi215001
KEGGimmu:215001
UCSCiuc008bcm.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC159277 Genomic DNA No translation available.
BC026460 mRNA Translation: AAH26460.1
CCDSiCCDS50038.1
RefSeqiNP_001093924.1, NM_001100454.1
UniGeneiMm.328871

3D structure databases

ProteinModelPortaliQ8R0S6
SMRiQ8R0S6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093141

Protein family/group databases

MEROPSiI02.953

PTM databases

PhosphoSitePlusiQ8R0S6

Proteomic databases

PaxDbiQ8R0S6
PRIDEiQ8R0S6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095487; ENSMUSP00000093141; ENSMUSG00000071192
GeneIDi215001
KEGGimmu:215001
UCSCiuc008bcm.2 mouse

Organism-specific databases

CTDi117166
MGIiMGI:2670967 Wfikkn1

Phylogenomic databases

eggNOGiKOG4597 Eukaryota
ENOG41102GD LUCA
GeneTreeiENSGT00740000114929
HOGENOMiHOG000185176
HOVERGENiHBG108676
InParanoidiQ8R0S6
OMAiWEPRWAY
OrthoDBiEOG091G09P2
PhylomeDBiQ8R0S6
TreeFamiTF315349

Miscellaneous databases

PROiPR:Q8R0S6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071192 Expressed in 83 organ(s), highest expression level in ear vesicle
CleanExiMM_WFIKKN1
ExpressionAtlasiQ8R0S6 baseline and differential
GenevisibleiQ8R0S6 MM

Family and domain databases

CDDicd00109 KU, 1 hit
Gene3Di2.60.40.10, 1 hit
4.10.410.10, 2 hits
4.10.75.10, 1 hit
InterProiView protein in InterPro
IPR036645 Elafin-like_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR001134 Netrin_domain
IPR020901 Prtase_inh_Kunz-CS
IPR008993 TIMP-like_OB-fold
IPR008197 WAP_dom
IPR033638 WFIKKN1
PANTHERiPTHR10083:SF212 PTHR10083:SF212, 1 hit
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 2 hits
PF00095 WAP, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00408 IGc2, 1 hit
SM00131 KU, 2 hits
SM00217 WAP, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
SSF48726 SSF48726, 1 hit
SSF50242 SSF50242, 1 hit
SSF57256 SSF57256, 1 hit
SSF57362 SSF57362, 2 hits
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 1 hit
PS50279 BPTI_KUNITZ_2, 2 hits
PS50835 IG_LIKE, 1 hit
PS51465 KAZAL_2, 1 hit
PS50189 NTR, 1 hit
PS51390 WAP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWFKN1_MOUSE
AccessioniPrimary (citable) accession number: Q8R0S6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: November 7, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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