Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

General transcription factor IIF subunit 2

Gene

Gtf2f2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-6803529 FGFR2 alternative splicing
R-MMU-6807505 RNA polymerase II transcribes snRNA genes
R-MMU-72086 mRNA Capping
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway
R-MMU-72203 Processing of Capped Intron-Containing Pre-mRNA
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIF subunit 2 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase GTF2F2
Transcription initiation factor IIF subunit beta
Short name:
TFIIF-beta
Gene namesi
Name:Gtf2f2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1915955 Gtf2f2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002112362 – 249General transcription factor IIF subunit 2Add BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei22N6-acetyllysineBy similarity1
Modified residuei33N6-acetyllysineBy similarity1
Modified residuei137N6-acetyllysineCombined sources1
Modified residuei142PhosphoserineBy similarity1
Modified residuei248PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8R0A0
MaxQBiQ8R0A0
PaxDbiQ8R0A0
PRIDEiQ8R0A0

PTM databases

iPTMnetiQ8R0A0
PhosphoSitePlusiQ8R0A0

Expressioni

Gene expression databases

BgeeiENSMUSG00000067995 Expressed in 282 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_GTF2F2
ExpressionAtlasiQ8R0A0 baseline and differential
GenevisibleiQ8R0A0 MM

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Interacts with HTATSF1 and URI1 (By similarity). Interacts with GPBP1. Interacts with GTF2B (via N-terminus); this interaction is inhibited in presence of GTF2F1 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi213003, 1 interactor
ComplexPortaliCPX-83 Transcription factor TFIIF complex
IntActiQ8R0A0, 1 interactor
MINTiQ8R0A0
STRINGi10090.ENSMUSP00000086312

Structurei

3D structure databases

ProteinModelPortaliQ8R0A0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIF beta subunit family.Curated

Phylogenomic databases

eggNOGiKOG2905 Eukaryota
COG5090 LUCA
GeneTreeiENSGT00390000016051
HOGENOMiHOG000294179
HOVERGENiHBG001606
InParanoidiQ8R0A0
KOiK03139
OMAiNMWELKK
OrthoDBiEOG091G0IN2
PhylomeDBiQ8R0A0
TreeFamiTF314290

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR003196 TFIIF_beta
IPR011039 TFIIF_interaction
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10445 PTHR10445, 1 hit
PfamiView protein in Pfam
PF02270 TFIIF_beta, 1 hit
PIRSFiPIRSF015849 TFIIF-beta, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50916 SSF50916, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R0A0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKNQG
60 70 80 90 100
RTEVSFTLNE DLANIHDIGG KPASVSAPRE HPFVLQSVGG QTLTVFTESS
110 120 130 140 150
SDKLSLEGIV VQRAECRPAA SENYMKLKRL QIEESSKPVR LSQQLDKVVT
160 170 180 190 200
TNYKPVANHQ YNIEYERKKK EDGKRARADK QHVLDMLFSA FEKHQYYNLK
210 220 230 240
DLVDITKQPV GYLKEILKEI GIQNVKGIHK NTWELKPEYR HYQTEEKSD
Length:249
Mass (Da):28,381
Last modified:June 1, 2002 - v1
Checksum:iE125CD762A42EB14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027173 mRNA Translation: AAH27173.1
CCDSiCCDS36981.1
RefSeqiNP_081092.1, NM_026816.3
UniGeneiMm.20415

Genome annotation databases

EnsembliENSMUST00000088922; ENSMUSP00000086312; ENSMUSG00000067995
GeneIDi68705
KEGGimmu:68705
UCSCiuc007urb.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027173 mRNA Translation: AAH27173.1
CCDSiCCDS36981.1
RefSeqiNP_081092.1, NM_026816.3
UniGeneiMm.20415

3D structure databases

ProteinModelPortaliQ8R0A0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213003, 1 interactor
ComplexPortaliCPX-83 Transcription factor TFIIF complex
IntActiQ8R0A0, 1 interactor
MINTiQ8R0A0
STRINGi10090.ENSMUSP00000086312

PTM databases

iPTMnetiQ8R0A0
PhosphoSitePlusiQ8R0A0

Proteomic databases

EPDiQ8R0A0
MaxQBiQ8R0A0
PaxDbiQ8R0A0
PRIDEiQ8R0A0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000088922; ENSMUSP00000086312; ENSMUSG00000067995
GeneIDi68705
KEGGimmu:68705
UCSCiuc007urb.1 mouse

Organism-specific databases

CTDi2963
MGIiMGI:1915955 Gtf2f2

Phylogenomic databases

eggNOGiKOG2905 Eukaryota
COG5090 LUCA
GeneTreeiENSGT00390000016051
HOGENOMiHOG000294179
HOVERGENiHBG001606
InParanoidiQ8R0A0
KOiK03139
OMAiNMWELKK
OrthoDBiEOG091G0IN2
PhylomeDBiQ8R0A0
TreeFamiTF314290

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-6803529 FGFR2 alternative splicing
R-MMU-6807505 RNA polymerase II transcribes snRNA genes
R-MMU-72086 mRNA Capping
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway
R-MMU-72203 Processing of Capped Intron-Containing Pre-mRNA
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-MMU-9018519 Estrogen-dependent gene expression

Miscellaneous databases

ChiTaRSiGtf2f2 mouse
PROiPR:Q8R0A0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000067995 Expressed in 282 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_GTF2F2
ExpressionAtlasiQ8R0A0 baseline and differential
GenevisibleiQ8R0A0 MM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR003196 TFIIF_beta
IPR011039 TFIIF_interaction
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10445 PTHR10445, 1 hit
PfamiView protein in Pfam
PF02270 TFIIF_beta, 1 hit
PIRSFiPIRSF015849 TFIIF-beta, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50916 SSF50916, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiT2FB_MOUSE
AccessioniPrimary (citable) accession number: Q8R0A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again