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Protein

Beta-1,4-galactosyltransferase 7

Gene

B4galt7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for the biosynthesis of the tetrasaccharide linkage region of proteoglycans, especially for small proteoglycans in skin fibroblasts.By similarity

Catalytic activityi

UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein] = UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein].

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi165ManganeseBy similarity1
Binding sitei194UDP-alpha-D-galactoseBy similarity1
Binding sitei224UDP-alpha-D-galactoseBy similarity1
Metal bindingi257Manganese; via tele nitrogenBy similarity1
Binding sitei266UDP-alpha-D-galactoseBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1971475 A tetrasaccharide linker sequence is required for GAG synthesis
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT7 Glycosyltransferase Family 7

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 7 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 7
Short name:
Beta4Gal-T7
Short name:
b4Gal-T7
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 7
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 7
Including the following 1 domains:
Xylosylprotein 4-beta-galactosyltransferase (EC:2.4.1.133)
Alternative name(s):
Proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I
UDP-galactose:beta-xylose beta-1,4-galactosyltransferase
XGPT
XGalT-1
Xylosylprotein beta-1,4-galactosyltransferase
Gene namesi
Name:B4galt7
Synonyms:Xgalt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2384987 B4galt7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicSequence analysisAdd BLAST30
Transmembranei31 – 51Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini52 – 327LumenalSequence analysisAdd BLAST276

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805511 – 327Beta-1,4-galactosyltransferase 7Add BLAST327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi154N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8R087
PRIDEiQ8R087

Expressioni

Gene expression databases

BgeeiENSMUSG00000021504
ExpressionAtlasiQ8R087 baseline and differential
GenevisibleiQ8R087 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000068532

Structurei

3D structure databases

ProteinModelPortaliQ8R087
SMRiQ8R087
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 104UDP-alpha-D-galactose bindingBy similarity5
Regioni139 – 141UDP-alpha-D-galactose bindingBy similarity3
Regioni164 – 165UDP-alpha-D-galactose bindingBy similarity2
Regioni226 – 229N-acetyl-D-glucosamine bindingBy similarity4
Regioni257 – 259UDP-alpha-D-galactose bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3917 Eukaryota
ENOG410Z0TP LUCA
GeneTreeiENSGT00760000119140
HOGENOMiHOG000286021
HOVERGENiHBG050654
InParanoidiQ8R087
KOiK00733
OMAiKAATPWC
OrthoDBiEOG091G0HCH
PhylomeDBiQ8R087
TreeFamiTF312834

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR003859 Galactosyl_T
IPR027791 Galactosyl_T_C
IPR027995 Galactosyl_T_N
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR19300 PTHR19300, 1 hit
PfamiView protein in Pfam
PF02709 Glyco_transf_7C, 1 hit
PF13733 Glyco_transf_7N, 1 hit
PRINTSiPR02050 B14GALTRFASE
SUPFAMiSSF53448 SSF53448, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R087-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPSRRKAAQ LPWEDGRARL LPGGLRRKCS IFHLFIAFLL LVFFSLLWLQ
60 70 80 90 100
LSCSGDMAQV TRGQGQETSG PPRACPPEPP PEHWEEDESW GPHRLAVLVP
110 120 130 140 150
FRERFEELLV FVPHMHRFLS RKRIQHHIYV LNQVDHFRFN RAALINVGFL
160 170 180 190 200
ESSNSTDYIA MHDVDLLPLN EELDYGFPEA GPFHVASPEL HPLYHYKTYV
210 220 230 240 250
GGILLLSKQH YQLCNGMSNR FWGWGREDDE FYRRIKGAGL QLFRPSGITT
260 270 280 290 300
GYQTFRHLHD PAWRKRDQKR IAAQKQEQFK VDREGGLNTV KYRVDSRTAL
310 320
SIGGAPCTVL NVMLDCDKTA TPWCIFG
Length:327
Mass (Da):37,754
Last modified:June 1, 2002 - v1
Checksum:iF79B656325DCFDE0
GO
Isoform 2 (identifier: Q8R087-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-275: Missing.

Show »
Length:292
Mass (Da):33,563
Checksum:iD50E046DBEF4D599
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014231241 – 275Missing in isoform 2. 1 PublicationAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027195 mRNA Translation: AAH27195.1
BC055703 mRNA Translation: AAH55703.1
CCDSiCCDS26550.1 [Q8R087-1]
CCDS79190.1 [Q8R087-2]
RefSeqiNP_001298066.1, NM_001311137.1 [Q8R087-2]
NP_666157.1, NM_146045.2 [Q8R087-1]
UniGeneiMm.139825

Genome annotation databases

EnsembliENSMUST00000064701; ENSMUSP00000068532; ENSMUSG00000021504 [Q8R087-1]
ENSMUST00000100764; ENSMUSP00000098327; ENSMUSG00000021504 [Q8R087-2]
GeneIDi218271
KEGGimmu:218271
UCSCiuc007qru.1 mouse [Q8R087-1]
uc011yzw.1 mouse [Q8R087-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiB4GT7_MOUSE
AccessioniPrimary (citable) accession number: Q8R087
Secondary accession number(s): Q7TNU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health