UniProtKB - Q8R081 (HNRPL_MOUSE)
Protein
Heterogeneous nuclear ribonucleoprotein L
Gene
Hnrnpl
Organism
Mus musculus (Mouse)
Status
Functioni
Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion (PubMed:22523384). Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.By similarity1 Publication
GO - Molecular functioni
- mRNA 3'-UTR binding Source: MGI
- mRNA binding Source: GO_Central
- mRNA CDS binding Source: MGI
- pre-mRNA intronic binding Source: UniProtKB
- RNA binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- cellular response to amino acid starvation Source: MGI
- circadian rhythm Source: Ensembl
- mRNA processing Source: InterPro
- negative regulation of mRNA splicing, via spliceosome Source: MGI
- positive regulation of mRNA binding Source: MGI
- positive regulation of translation Source: MGI
- regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
- regulation of RNA splicing Source: GO_Central
- regulation of translation Source: GO_Central
- response to peptide Source: Ensembl
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72203, Processing of Capped Intron-Containing Pre-mRNA |
Names & Taxonomyi
Protein namesi | Recommended name: Heterogeneous nuclear ribonucleoprotein LShort name: hnRNP L |
Gene namesi | Name:Hnrnpl Synonyms:Hnrpl |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104816, Hnrnpl |
Subcellular locationi
Nucleus
- nucleoplasm By similarity
Other locations
- Cytoplasm By similarity
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. These granules are not identical with P bodies or stress granules.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: UniProtKB
- nucleus Source: MGI
- pronucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- perinuclear region of cytoplasm Source: MGI
- ribonucleoprotein complex Source: UniProtKB
- ribonucleoprotein granule Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Complete lethality during early embryonic development.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081863 | 1 – 586 | Heterogeneous nuclear ribonucleoprotein LAdd BLAST | 586 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 59 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 62 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 98 | PhosphoserineBy similarity | 1 | |
Cross-linki | 133 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 182 | PhosphoserineBy similarity | 1 | |
Modified residuei | 266 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 288 | PhosphoserineBy similarity | 1 | |
Modified residuei | 295 | PhosphoserineBy similarity | 1 | |
Cross-linki | 299 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 351 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 355 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 378 | PhosphoserineCombined sources | 1 | |
Modified residuei | 541 | Phosphoserine; by CaMK4By similarity | 1 | |
Cross-linki | 565 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Phosphorylation at Ser-541 by CaMK4 enhances interaction with a CaMK4-responsive RNA element (CaRRE1), and prevents inclusion of the stress axis-regulated exon (STREX) of the KCNMA1 potassium channel transcripts upon membrane depolarization.By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8R081 |
jPOSTi | Q8R081 |
MaxQBi | Q8R081 |
PaxDbi | Q8R081 |
PeptideAtlasi | Q8R081 |
PRIDEi | Q8R081 |
2D gel databases
REPRODUCTION-2DPAGEi | Q8R081 |
PTM databases
iPTMneti | Q8R081 |
PhosphoSitePlusi | Q8R081 |
SwissPalmi | Q8R081 |
Expressioni
Tissue specificityi
Detected in hematopoietic cells, including lymphoid progenitor cells.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000015165, Expressed in retinal neural layer and 310 other tissues |
Genevisiblei | Q8R081, MM |
Interactioni
Subunit structurei
Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.
Interacts with HNRNPLL.
Interacts with APEX1; the interaction is DNA-dependent.
Component of a complex with SETD2 (By similarity).
Interacts with ELAVL1 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 200360, 45 interactors |
IntActi | Q8R081, 13 interactors |
MINTi | Q8R081 |
STRINGi | 10090.ENSMUSP00000134734 |
Miscellaneous databases
RNActi | Q8R081, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q8R081 |
SMRi | Q8R081 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 99 – 173 | RRM 1PROSITE-ProRule annotationAdd BLAST | 75 | |
Domaini | 190 – 267 | RRM 2PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 379 – 476 | RRM 3PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 492 – 580 | RRM 4PROSITE-ProRule annotationAdd BLAST | 89 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 39 – 86 | Gly-richAdd BLAST | 48 | |
Compositional biasi | 332 – 379 | Pro-richAdd BLAST | 48 |
Domaini
RRM domain 2 has moderate RNA-binding affinity. RRM domains 3 and 4 may facilitate RNA looping when binding to two appropriately separated binding sites within the same target pre-mRNA.By similarity
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1456, Eukaryota |
GeneTreei | ENSGT00990000203586 |
InParanoidi | Q8R081 |
OrthoDBi | 1545178at2759 |
PhylomeDBi | Q8R081 |
Family and domain databases
CDDi | cd12780, RRM1_hnRNPL, 1 hit cd12785, RRM2_hnRNPL, 1 hit cd12699, RRM3_hnRNPL, 1 hit cd12704, RRM4_hnRNPL, 1 hit |
Gene3Di | 3.30.70.330, 4 hits |
InterProi | View protein in InterPro IPR006536, HnRNP-L/PTB IPR034816, hnRNP-L_RRM3 IPR035005, hnRNPL_RRM1 IPR035008, hnRNPL_RRM2 IPR034817, hnRNPL_RRM4 IPR012677, Nucleotide-bd_a/b_plait_sf IPR021790, PTBP1_RRM IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF11835, RRM_8, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 3 hits |
TIGRFAMsi | TIGR01649, hnRNP-L_PTB, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q8R081-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRRLLPRAE KRRRRLEQRQ QPDEQLRRAG AMVKMAAAGG GGGGGRYYGG
60 70 80 90 100
GNEGGRAPKR LKTENAGDQH GGGGGGGSGA AGGGGGENYD DPHKTPASPV
110 120 130 140 150
VHIRGLIDGV VEADLVEALQ EFGPISYVVV MPKKRQALVE FEDVLGACNA
160 170 180 190 200
VNYAADNQIY IAGHPAFVNY STSQKISRPG DSDDSRSVNS VLLFTILNPI
210 220 230 240 250
YSITTDVLYT ICNPCGPVQR IVIFRKNGVQ AMVEFDSVQS AQRAKASLNG
260 270 280 290 300
ADIYSGCCTL KIEYAKPTRL NVFKNDQDTW DYTNPNLSGQ GDPGSNPNKR
310 320 330 340 350
QRQPPLLGDH PAEYGGPHGG YHSHYHDEGY GPPPPHYEGR RMGPPVGGHR
360 370 380 390 400
RGPSRYGPQY GHPPPPPPPP DYGPHADSPV LMVYGLDQSK MNCDRVFNVF
410 420 430 440 450
CLYGNVEKVK FMKSKPGAAM VEMADGYAVD RAITHLNNNF MFGQKMNVCV
460 470 480 490 500
SKQPAIMPGQ SYGLEDGSCS YKDFSESRNN RFSTPEQAAK NRIQHPSNVL
510 520 530 540 550
HFFNAPLEVT EENFFEICDE LGVKRPTSVK VFSGKSERSS SGLLEWDSKS
560 570 580
DALETLGFLN HYQMKNPNGP YPYTLKLCFS TAQHAS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UY38 | G3UY38_MOUSE | Heterogeneous nuclear ribonucleopro... | Hnrnpl | 456 | Annotation score: | ||
G5E924 | G5E924_MOUSE | Heterogeneous nuclear ribonucleopro... | Hnrnpl | 615 | Annotation score: | ||
G3UY56 | G3UY56_MOUSE | Heterogeneous nuclear ribonucleopro... | Hnrnpl | 201 | Annotation score: | ||
G3UYY3 | G3UYY3_MOUSE | Heterogeneous nuclear ribonucleopro... | Hnrnpl | 112 | Annotation score: |
Sequence cautioni
The sequence AAH27206 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 388 | Q → E in BAA24237 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC027206 mRNA Translation: AAH27206.1 Different initiation. BC030461 mRNA Translation: AAH30461.1 BC099683 mRNA Translation: AAH99683.1 AB009392 mRNA Translation: BAA24237.1 |
CCDSi | CCDS39864.2 |
RefSeqi | NP_796275.3, NM_177301.5 XP_006539621.1, XM_006539558.2 |
Genome annotation databases
Ensembli | ENSMUST00000038572; ENSMUSP00000049407; ENSMUSG00000015165 ENSMUST00000174548; ENSMUSP00000133728; ENSMUSG00000015165 |
GeneIDi | 15388 |
KEGGi | mmu:15388 |
UCSCi | uc009fzz.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC027206 mRNA Translation: AAH27206.1 Different initiation. BC030461 mRNA Translation: AAH30461.1 BC099683 mRNA Translation: AAH99683.1 AB009392 mRNA Translation: BAA24237.1 |
CCDSi | CCDS39864.2 |
RefSeqi | NP_796275.3, NM_177301.5 XP_006539621.1, XM_006539558.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3S01 | X-ray | 2.15 | A | 376-586 | [»] | |
3TYT | X-ray | 1.60 | A | 376-579 | [»] | |
BMRBi | Q8R081 | |||||
SMRi | Q8R081 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200360, 45 interactors |
IntActi | Q8R081, 13 interactors |
MINTi | Q8R081 |
STRINGi | 10090.ENSMUSP00000134734 |
PTM databases
iPTMneti | Q8R081 |
PhosphoSitePlusi | Q8R081 |
SwissPalmi | Q8R081 |
2D gel databases
REPRODUCTION-2DPAGEi | Q8R081 |
Proteomic databases
EPDi | Q8R081 |
jPOSTi | Q8R081 |
MaxQBi | Q8R081 |
PaxDbi | Q8R081 |
PeptideAtlasi | Q8R081 |
PRIDEi | Q8R081 |
Protocols and materials databases
Antibodypediai | 4276, 431 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000038572; ENSMUSP00000049407; ENSMUSG00000015165 ENSMUST00000174548; ENSMUSP00000133728; ENSMUSG00000015165 |
GeneIDi | 15388 |
KEGGi | mmu:15388 |
UCSCi | uc009fzz.2, mouse |
Organism-specific databases
CTDi | 3191 |
MGIi | MGI:104816, Hnrnpl |
Phylogenomic databases
eggNOGi | KOG1456, Eukaryota |
GeneTreei | ENSGT00990000203586 |
InParanoidi | Q8R081 |
OrthoDBi | 1545178at2759 |
PhylomeDBi | Q8R081 |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72203, Processing of Capped Intron-Containing Pre-mRNA |
Miscellaneous databases
BioGRID-ORCSi | 15388, 8 hits in 18 CRISPR screens |
ChiTaRSi | Hnrnpl, mouse |
PROi | PR:Q8R081 |
RNActi | Q8R081, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015165, Expressed in retinal neural layer and 310 other tissues |
Genevisiblei | Q8R081, MM |
Family and domain databases
CDDi | cd12780, RRM1_hnRNPL, 1 hit cd12785, RRM2_hnRNPL, 1 hit cd12699, RRM3_hnRNPL, 1 hit cd12704, RRM4_hnRNPL, 1 hit |
Gene3Di | 3.30.70.330, 4 hits |
InterProi | View protein in InterPro IPR006536, HnRNP-L/PTB IPR034816, hnRNP-L_RRM3 IPR035005, hnRNPL_RRM1 IPR035008, hnRNPL_RRM2 IPR034817, hnRNPL_RRM4 IPR012677, Nucleotide-bd_a/b_plait_sf IPR021790, PTBP1_RRM IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF11835, RRM_8, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 3 hits |
TIGRFAMsi | TIGR01649, hnRNP-L_PTB, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HNRPL_MOUSE | |
Accessioni | Q8R081Primary (citable) accession number: Q8R081 Secondary accession number(s): O54789, Q499X2, Q8K0S7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | November 25, 2008 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references