UniProtKB - Q8R060 (ZWILC_MOUSE)
Protein
Protein zwilch homolog
Gene
Zwilch
Organism
Mus musculus (Mouse)
Status
Functioni
Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (By similarity).By similarity
GO - Biological processi
- cell division Source: UniProtKB-KW
- mitotic cell cycle checkpoint Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
Reactomei | R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-5663220, RHO GTPases Activate Formins R-MMU-68877, Mitotic Prometaphase R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein zwilch homolog |
Gene namesi | Name:Zwilch |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915264, Zwilch |
Subcellular locationi
Other locations
- kinetochore By similarity
Other locations
- condensed chromosome kinetochore Source: UniProtKB-SubCell
- kinetochore Source: MGI
- RZZ complex Source: MGI
Keywords - Cellular componenti
Centromere, Chromosome, KinetochorePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000314801 | 1 – 589 | Protein zwilch homologAdd BLAST | 589 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 88 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8R060 |
MaxQBi | Q8R060 |
PaxDbi | Q8R060 |
PRIDEi | Q8R060 |
PTM databases
iPTMneti | Q8R060 |
PhosphoSitePlusi | Q8R060 |
Interactioni
Subunit structurei
Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH; in the complex interacts directly with KNTC1/ROD (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 212596, 19 interactors |
IntActi | Q8R060, 2 interactors |
MINTi | Q8R060 |
STRINGi | 10090.ENSMUSP00000112790 |
Miscellaneous databases
RNActi | Q8R060, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ZWILCH family.Curated
Phylogenomic databases
eggNOGi | KOG4803, Eukaryota |
InParanoidi | Q8R060 |
OrthoDBi | 1451737at2759 |
PhylomeDBi | Q8R060 |
Family and domain databases
InterProi | View protein in InterPro IPR018630, Zwilch |
PANTHERi | PTHR15995, PTHR15995, 1 hit |
Pfami | View protein in Pfam PF09817, Zwilch, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All
Q8R060-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWSRMNRAAE EFYARLRQEF NEEKKGASKD PFIYEADVQV QLISKGQPSL
60 70 80 90 100
LKTILNENDS VFLVEKVVLE KEETSQVEEL QSEETAISDL SAGENIRPLA
110 120 130 140 150
LPVGRARQLI GLYTMAHNPN MTHLKIKQPV TALPPLWVRC DGSDPEGTCW
160 170 180 190 200
LGAELITTND IIAGVILYVL TCKADKNYSE DLENLKTSHK KRHHVSAVTA
210 220 230 240 250
RGFAQYELFK SDDLDDTVAP SQTTVTLDLS WSPVDEMLQT PPLSSTAALN
260 270 280 290 300
IRVQSGESRG CLSHLHRELK FLLVLADGIR TGVTEWLEPL ETKSALEFVQ
310 320 330 340 350
EFLNDLNKLD EFDDSTKKDK QKEAVNHDAA AVVRSMLLTV RGDLDFAEQL
360 370 380 390 400
WCRMSSSVVS YQDLVKCFTL ILQSLQRGDI QPWLHSGSNS LLSKLIHQSY
410 420 430 440 450
HGAMDSVPLS GTTPLQMLLE IGLDKLKKDY ISFFVSQELA SLNHLEYFIS
460 470 480 490 500
PSVSTQEQVC RVQKLHHILE ILVICMLFIK PQHELLFSLT QSCIKYYKQN
510 520 530 540 550
PLDEQHIFQL PVRPAAVKNL YQSEKPQKWR VELSNSQKRV KTVWQLSDSS
560 570 580
PVDHSSFHRP EFPELTLNGS LEERTAFVNM LTCSQVHFK
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3X9Z0 | G3X9Z0_MOUSE | Protein zwilch homolog | Zwilch | 596 | Annotation score: | ||
D3Z6K4 | D3Z6K4_MOUSE | Protein zwilch homolog | Zwilch | 154 | Annotation score: | ||
H3BKZ0 | H3BKZ0_MOUSE | Protein zwilch homolog | Zwilch | 475 | Annotation score: | ||
H3BJ52 | H3BJ52_MOUSE | Protein zwilch homolog | Zwilch | 572 | Annotation score: | ||
H3BKB9 | H3BKB9_MOUSE | Protein zwilch homolog | Zwilch | 303 | Annotation score: | ||
F6TCM4 | F6TCM4_MOUSE | Protein zwilch homolog | Zwilch | 51 | Annotation score: | ||
H3BL96 | H3BL96_MOUSE | Protein zwilch homolog | Zwilch | 38 | Annotation score: | ||
H3BIZ1 | H3BIZ1_MOUSE | Protein zwilch homolog | Zwilch | 86 | Annotation score: | ||
H3BJF6 | H3BJF6_MOUSE | Protein zwilch homolog | Zwilch | 82 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | E → G in BAB26358 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 215 | D → V in BAB26358 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 285 | E → G in BAB26358 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK009559 mRNA Translation: BAB26358.2 AK019825 mRNA Translation: BAB31869.2 BC027435 mRNA Translation: AAH27435.1 |
RefSeqi | NP_080783.3, NM_026507.4 |
Genome annotation databases
GeneIDi | 68014 |
KEGGi | mmu:68014 |
UCSCi | uc012gva.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK009559 mRNA Translation: BAB26358.2 AK019825 mRNA Translation: BAB31869.2 BC027435 mRNA Translation: AAH27435.1 |
RefSeqi | NP_080783.3, NM_026507.4 |
3D structure databases
SMRi | Q8R060 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 212596, 19 interactors |
IntActi | Q8R060, 2 interactors |
MINTi | Q8R060 |
STRINGi | 10090.ENSMUSP00000112790 |
PTM databases
iPTMneti | Q8R060 |
PhosphoSitePlusi | Q8R060 |
Proteomic databases
EPDi | Q8R060 |
MaxQBi | Q8R060 |
PaxDbi | Q8R060 |
PRIDEi | Q8R060 |
Genome annotation databases
GeneIDi | 68014 |
KEGGi | mmu:68014 |
UCSCi | uc012gva.1, mouse |
Organism-specific databases
CTDi | 55055 |
MGIi | MGI:1915264, Zwilch |
Phylogenomic databases
eggNOGi | KOG4803, Eukaryota |
InParanoidi | Q8R060 |
OrthoDBi | 1451737at2759 |
PhylomeDBi | Q8R060 |
Enzyme and pathway databases
Reactomei | R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-5663220, RHO GTPases Activate Formins R-MMU-68877, Mitotic Prometaphase R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Miscellaneous databases
BioGRID-ORCSi | 68014, 5 hits in 17 CRISPR screens |
ChiTaRSi | Zwilch, mouse |
PROi | PR:Q8R060 |
RNActi | Q8R060, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR018630, Zwilch |
PANTHERi | PTHR15995, PTHR15995, 1 hit |
Pfami | View protein in Pfam PF09817, Zwilch, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZWILC_MOUSE | |
Accessioni | Q8R060Primary (citable) accession number: Q8R060 Secondary accession number(s): Q9D2E4, Q9D761 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families