UniProtKB - Q8R059 (GALE_MOUSE)
Protein
UDP-glucose 4-epimerase
Gene
Gale
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.By similarity
Catalytic activityi
Cofactori
NAD+By similarity
: galactose metabolism Pathwayi
This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 87 | NAD; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 91 | NADBy similarity | 1 | |
Active sitei | 156 | Proton acceptorBy similarity | 1 | |
Binding sitei | 160 | NADBy similarity | 1 | |
Binding sitei | 184 | NADBy similarity | 1 | |
Binding sitei | 238 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 13 | NADBy similarity | 3 | |
Nucleotide bindingi | 32 – 36 | NADBy similarity | 5 | |
Nucleotide bindingi | 65 – 66 | NADBy similarity | 2 |
GO - Molecular functioni
- identical protein binding Source: MGI
- protein homodimerization activity Source: MGI
- UDP-glucose 4-epimerase activity Source: MGI
- UDP-N-acetylglucosamine 4-epimerase activity Source: UniProtKB-EC
GO - Biological processi
- galactose catabolic process Source: MGI
- galactose catabolic process via UDP-galactose Source: GO_Central
- galactose metabolic process Source: MGI
- glycolytic process from galactose Source: MGI
Keywordsi
Molecular function | Isomerase |
Biological process | Carbohydrate metabolism, Galactose metabolism |
Ligand | NAD |
Enzyme and pathway databases
Reactomei | R-MMU-70370, Galactose catabolism |
UniPathwayi | UPA00214 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-glucose 4-epimeraseBy similarity (EC:5.1.3.2By similarity)Alternative name(s): GalactowaldenaseBy similarity UDP-N-acetylglucosamine 4-epimeraseBy similarity (EC:5.1.3.7By similarity) Short name: UDP-GlcNAc 4-epimeraseBy similarity UDP-galactosamine 4-epimeraseBy similarity Short name: UDP-GalNAc 4-epimeraseBy similarity UDP-galactose 4-epimeraseBy similarity |
Gene namesi | Name:Gale |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921496, Gale |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000183190 | 1 – 347 | UDP-glucose 4-epimeraseAdd BLAST | 347 |
Proteomic databases
EPDi | Q8R059 |
jPOSTi | Q8R059 |
MaxQBi | Q8R059 |
PaxDbi | Q8R059 |
PeptideAtlasi | Q8R059 |
PRIDEi | Q8R059 |
PTM databases
iPTMneti | Q8R059 |
PhosphoSitePlusi | Q8R059 |
SwissPalmi | Q8R059 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028671, Expressed in colon and 222 other tissues |
ExpressionAtlasi | Q8R059, baseline and differential |
Genevisiblei | Q8R059, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 216604, 1 interactor |
IntActi | Q8R059, 2 interactors |
MINTi | Q8R059 |
STRINGi | 10090.ENSMUSP00000099599 |
Miscellaneous databases
RNActi | Q8R059, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 131 – 133 | Substrate bindingBy similarity | 3 | |
Regioni | 184 – 186 | Substrate bindingBy similarity | 3 | |
Regioni | 205 – 207 | Substrate bindingBy similarity | 3 | |
Regioni | 223 – 225 | Substrate bindingBy similarity | 3 | |
Regioni | 299 – 302 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the NAD(P)-dependent epimerase/dehydratase family.Curated
Phylogenomic databases
eggNOGi | KOG1371, Eukaryota |
GeneTreei | ENSGT00940000158000 |
HOGENOMi | CLU_007383_1_10_1 |
InParanoidi | Q8R059 |
OMAi | GEHLICN |
OrthoDBi | 662484at2759 |
PhylomeDBi | Q8R059 |
TreeFami | TF105800 |
Family and domain databases
CDDi | cd05247, UDP_G4E_1_SDR_e, 1 hit |
InterProi | View protein in InterPro IPR016040, NAD(P)-bd_dom IPR036291, NAD(P)-bd_dom_sf IPR005886, UDP_G4E |
Pfami | View protein in Pfam PF16363, GDP_Man_Dehyd, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR01179, galE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8R059-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKVLVTGGA GYIGSHTVLE LLEAGYSPVV IDNFHNAIRG EDSMPESLRR
60 70 80 90 100
VQELTGRSVE FEEMDILDQA ALQHLFKKHS FKAVIHFAGL KAVGESVQKP
110 120 130 140 150
LDYYRVNLTG TIQLLEIMRA HGVKNLVFSS SATVYGNPQY LPLDEAHPTG
160 170 180 190 200
GCTNPYGKSK FFIEEMIRDL CRADTAWNAV LLRYFNPIGA HASGRIGEDP
210 220 230 240 250
QGIPNNLMPY VSQVAIGRRE ALNVFGDDYA TEDGTGVRDY IHVVDLAKGH
260 270 280 290 300
IAALKKLKEQ CGCRTYNLGT GTGYSVLQMV QAMEKASGKK IPYKVVARRE
310 320 330 340
GDVAACYANP SLAHEELGWT AALGLDRMCE DLWRWQKQNP SGFGAQA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1AV53 | B1AV53_MOUSE | UDP-glucose 4-epimerase | Gale | 57 | Annotation score: | ||
B1AV54 | B1AV54_MOUSE | UDP-glucose 4-epimerase | Gale | 60 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC027438 mRNA Translation: AAH27438.1 |
CCDSi | CCDS18796.1 |
RefSeqi | NP_848476.1, NM_178389.3 XP_006539280.1, XM_006539217.1 XP_006539281.1, XM_006539218.3 XP_006539282.1, XM_006539219.3 |
Genome annotation databases
Ensembli | ENSMUST00000102540; ENSMUSP00000099599; ENSMUSG00000028671 ENSMUST00000102541; ENSMUSP00000099600; ENSMUSG00000028671 |
GeneIDi | 74246 |
UCSCi | uc008vhk.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC027438 mRNA Translation: AAH27438.1 |
CCDSi | CCDS18796.1 |
RefSeqi | NP_848476.1, NM_178389.3 XP_006539280.1, XM_006539217.1 XP_006539281.1, XM_006539218.3 XP_006539282.1, XM_006539219.3 |
3D structure databases
SMRi | Q8R059 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 216604, 1 interactor |
IntActi | Q8R059, 2 interactors |
MINTi | Q8R059 |
STRINGi | 10090.ENSMUSP00000099599 |
PTM databases
iPTMneti | Q8R059 |
PhosphoSitePlusi | Q8R059 |
SwissPalmi | Q8R059 |
Proteomic databases
EPDi | Q8R059 |
jPOSTi | Q8R059 |
MaxQBi | Q8R059 |
PaxDbi | Q8R059 |
PeptideAtlasi | Q8R059 |
PRIDEi | Q8R059 |
Protocols and materials databases
Antibodypediai | 1527, 294 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000102540; ENSMUSP00000099599; ENSMUSG00000028671 ENSMUST00000102541; ENSMUSP00000099600; ENSMUSG00000028671 |
GeneIDi | 74246 |
UCSCi | uc008vhk.1, mouse |
Organism-specific databases
CTDi | 2582 |
MGIi | MGI:1921496, Gale |
Phylogenomic databases
eggNOGi | KOG1371, Eukaryota |
GeneTreei | ENSGT00940000158000 |
HOGENOMi | CLU_007383_1_10_1 |
InParanoidi | Q8R059 |
OMAi | GEHLICN |
OrthoDBi | 662484at2759 |
PhylomeDBi | Q8R059 |
TreeFami | TF105800 |
Enzyme and pathway databases
UniPathwayi | UPA00214 |
Reactomei | R-MMU-70370, Galactose catabolism |
Miscellaneous databases
BioGRID-ORCSi | 74246, 8 hits in 20 CRISPR screens |
ChiTaRSi | Gale, mouse |
PROi | PR:Q8R059 |
RNActi | Q8R059, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028671, Expressed in colon and 222 other tissues |
ExpressionAtlasi | Q8R059, baseline and differential |
Genevisiblei | Q8R059, MM |
Family and domain databases
CDDi | cd05247, UDP_G4E_1_SDR_e, 1 hit |
InterProi | View protein in InterPro IPR016040, NAD(P)-bd_dom IPR036291, NAD(P)-bd_dom_sf IPR005886, UDP_G4E |
Pfami | View protein in Pfam PF16363, GDP_Man_Dehyd, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR01179, galE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GALE_MOUSE | |
Accessioni | Q8R059Primary (citable) accession number: Q8R059 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2003 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families