UniProtKB - Q8R016 (BLMH_MOUSE)
Protein
Bleomycin hydrolase
Gene
Blmh
Organism
Mus musculus (Mouse)
Status
Functioni
The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.By similarity
Catalytic activityi
- Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred. EC:3.4.22.40
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 73 | PROSITE-ProRule annotation | 1 | |
Active sitei | 372 | PROSITE-ProRule annotation | 1 | |
Active sitei | 396 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: UniProtKB-EC
- cysteine-type peptidase activity Source: GO_Central
- identical protein binding Source: MGI
- peptidase activity Source: MGI
GO - Biological processi
- homocysteine catabolic process Source: GO_Central
- response to drug Source: MGI
- response to toxic substance Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Enzyme and pathway databases
BRENDAi | 3.4.22.40, 3474 |
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Protein family/group databases
MEROPSi | C01.084 |
Names & Taxonomyi
Protein namesi | Recommended name: Bleomycin hydrolase (EC:3.4.22.40)Short name: BH Short name: BLM hydrolase Short name: BMH |
Gene namesi | Name:Blmh |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1345186, Blmh |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050551 | 1 – 455 | Bleomycin hydrolaseAdd BLAST | 455 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 391 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
CPTACi | non-CPTAC-3322 |
EPDi | Q8R016 |
jPOSTi | Q8R016 |
MaxQBi | Q8R016 |
PaxDbi | Q8R016 |
PeptideAtlasi | Q8R016 |
PRIDEi | Q8R016 |
PTM databases
iPTMneti | Q8R016 |
PhosphoSitePlusi | Q8R016 |
SwissPalmi | Q8R016 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020840, Expressed in zone of skin and 308 other tissues |
ExpressionAtlasi | Q8R016, baseline and differential |
Genevisiblei | Q8R016, MM |
Interactioni
Subunit structurei
Homohexamer.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 222434, 12 interactors |
IntActi | Q8R016, 2 interactors |
MINTi | Q8R016 |
STRINGi | 10090.ENSMUSP00000021197 |
Miscellaneous databases
RNActi | Q8R016, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C1 family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG4128, Eukaryota |
GeneTreei | ENSGT00390000001735 |
HOGENOMi | CLU_038600_0_0_1 |
InParanoidi | Q8R016 |
OMAi | WDMIVNL |
OrthoDBi | 649145at2759 |
PhylomeDBi | Q8R016 |
TreeFami | TF323372 |
Family and domain databases
CDDi | cd00585, Peptidase_C1B, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR004134, Peptidase_C1B |
PANTHERi | PTHR10363, PTHR10363, 1 hit |
Pfami | View protein in Pfam PF03051, Peptidase_C1_2, 1 hit |
PIRSFi | PIRSF005700, PepC, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00139, THIOL_PROTEASE_CYS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q8R016-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNNAGLNSEK VSALIQKLNS DPQFVLAQNV GTTHDLLDIC LRRATVQGAQ
60 70 80 90 100
HVFQHVVPQE GKPVTNQKSS GRCWIFSCLN VMRLPFMKKF NIEEFEFSQS
110 120 130 140 150
YLFFWDKVER CYFFLNAFVD TAQKKEPEDG RLVQYLLMNP TNDGGQWDML
160 170 180 190 200
VNIVEKYGVV PKKCFPESHT TEATRRMNDI LNHKMREFCI RLRNLVHSGA
210 220 230 240 250
TKGEISSTQD AMMEEIFRVV CICLGNPPET FTWEYRDKDK NYHKIGPITP
260 270 280 290 300
LQFYKEHVKP LFNMEDKICF VNDPRPQHKY NKLYTVDYLS NMVGGRKTLY
310 320 330 340 350
NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNGKLGLSD MNVYDHELVF
360 370 380 390 400
GVSLKNMNKA ERLAFGESLM THAMTFTAVS EKDNQEGTFV KWRVENSWGE
410 420 430 440 450
DHGHKGYLCM TDEWFSEYVY EVVVDKKHVP EEVLAVLEQE PIVLPAWDPM
GALAE
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PZH4 | E9PZH4_MOUSE | Bleomycin hydrolase | Blmh | 209 | Annotation score: | ||
E9PY26 | E9PY26_MOUSE | Bleomycin hydrolase | Blmh | 179 | Annotation score: | ||
E9PYU0 | E9PYU0_MOUSE | Bleomycin hydrolase | Blmh | 57 | Annotation score: | ||
E9QA53 | E9QA53_MOUSE | Bleomycin hydrolase | Blmh | 129 | Annotation score: | ||
E9Q6V3 | E9Q6V3_MOUSE | Bleomycin hydrolase | Blmh | 71 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 16 | Q → K in BAC28989 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 151 | V → A in AAH24090 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 391 | K → R in BAC30706 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK035228 mRNA Translation: BAC28989.1 AK040799 mRNA Translation: BAC30706.1 AK051441 mRNA Translation: BAC34639.1 AK053872 mRNA Translation: BAC35568.1 AK150438 mRNA Translation: BAE29560.1 AK167325 mRNA Translation: BAE39427.1 AL603842 Genomic DNA No translation available. BC024090 mRNA Translation: AAH24090.1 BC027037 mRNA Translation: AAH27037.1 BC027362 mRNA Translation: AAH27362.1 BC027403 mRNA Translation: AAH27403.1 |
CCDSi | CCDS25073.1 |
RefSeqi | NP_848760.1, NM_178645.4 |
Genome annotation databases
Ensembli | ENSMUST00000021197; ENSMUSP00000021197; ENSMUSG00000020840 |
GeneIDi | 104184 |
KEGGi | mmu:104184 |
UCSCi | uc007kge.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK035228 mRNA Translation: BAC28989.1 AK040799 mRNA Translation: BAC30706.1 AK051441 mRNA Translation: BAC34639.1 AK053872 mRNA Translation: BAC35568.1 AK150438 mRNA Translation: BAE29560.1 AK167325 mRNA Translation: BAE39427.1 AL603842 Genomic DNA No translation available. BC024090 mRNA Translation: AAH24090.1 BC027037 mRNA Translation: AAH27037.1 BC027362 mRNA Translation: AAH27362.1 BC027403 mRNA Translation: AAH27403.1 |
CCDSi | CCDS25073.1 |
RefSeqi | NP_848760.1, NM_178645.4 |
3D structure databases
SMRi | Q8R016 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 222434, 12 interactors |
IntActi | Q8R016, 2 interactors |
MINTi | Q8R016 |
STRINGi | 10090.ENSMUSP00000021197 |
Protein family/group databases
MEROPSi | C01.084 |
PTM databases
iPTMneti | Q8R016 |
PhosphoSitePlusi | Q8R016 |
SwissPalmi | Q8R016 |
Proteomic databases
CPTACi | non-CPTAC-3322 |
EPDi | Q8R016 |
jPOSTi | Q8R016 |
MaxQBi | Q8R016 |
PaxDbi | Q8R016 |
PeptideAtlasi | Q8R016 |
PRIDEi | Q8R016 |
Protocols and materials databases
Antibodypediai | 15119, 254 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000021197; ENSMUSP00000021197; ENSMUSG00000020840 |
GeneIDi | 104184 |
KEGGi | mmu:104184 |
UCSCi | uc007kge.2, mouse |
Organism-specific databases
CTDi | 642 |
MGIi | MGI:1345186, Blmh |
Phylogenomic databases
eggNOGi | KOG4128, Eukaryota |
GeneTreei | ENSGT00390000001735 |
HOGENOMi | CLU_038600_0_0_1 |
InParanoidi | Q8R016 |
OMAi | WDMIVNL |
OrthoDBi | 649145at2759 |
PhylomeDBi | Q8R016 |
TreeFami | TF323372 |
Enzyme and pathway databases
BRENDAi | 3.4.22.40, 3474 |
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 104184, 1 hit in 17 CRISPR screens |
ChiTaRSi | Blmh, mouse |
PROi | PR:Q8R016 |
RNActi | Q8R016, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020840, Expressed in zone of skin and 308 other tissues |
ExpressionAtlasi | Q8R016, baseline and differential |
Genevisiblei | Q8R016, MM |
Family and domain databases
CDDi | cd00585, Peptidase_C1B, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR004134, Peptidase_C1B |
PANTHERi | PTHR10363, PTHR10363, 1 hit |
Pfami | View protein in Pfam PF03051, Peptidase_C1_2, 1 hit |
PIRSFi | PIRSF005700, PepC, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00139, THIOL_PROTEASE_CYS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BLMH_MOUSE | |
Accessioni | Q8R016Primary (citable) accession number: Q8R016 Secondary accession number(s): Q3TJR8 Q8CID9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries