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Protein

Splicing factor 3B subunit 4

Gene

Sf3b4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.By similarity

GO - Molecular functioni

  • RNA binding Source: GO_Central

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • positive regulation of mRNA splicing, via spliceosome Source: MGI
  • RNA splicing Source: UniProtKB-KW

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3B subunit 4
Gene namesi
Name:Sf3b4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:109580 Sf3b4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003285852 – 424Splicing factor 3B subunit 4Add BLAST423

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei56PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8QZY9
MaxQBiQ8QZY9
PaxDbiQ8QZY9
PeptideAtlasiQ8QZY9
PRIDEiQ8QZY9

PTM databases

iPTMnetiQ8QZY9
PhosphoSitePlusiQ8QZY9

Expressioni

Gene expression databases

BgeeiENSMUSG00000068856 Expressed in 33 organ(s), highest expression level in spleen
GenevisibleiQ8QZY9 MM

Interactioni

Subunit structurei

Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts directly with SF3B2. Found in a complex with PRMT9, SF3B2 and SF3B4. The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.By similarity

Protein-protein interaction databases

BioGridi223499, 2 interactors
IntActiQ8QZY9, 8 interactors
MINTiQ8QZY9
STRINGi10090.ENSMUSP00000075709

Structurei

3D structure databases

ProteinModelPortaliQ8QZY9
SMRiQ8QZY9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 91RRM 1PROSITE-ProRule annotationAdd BLAST79
Domaini100 – 179RRM 2PROSITE-ProRule annotationAdd BLAST80

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi215 – 218Poly-Pro4
Compositional biasi262 – 268Poly-Pro7

Sequence similaritiesi

Belongs to the SF3B4 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0131 Eukaryota
ENOG410XPT4 LUCA
GeneTreeiENSGT00870000136537
HOGENOMiHOG000200535
HOVERGENiHBG002295
InParanoidiQ8QZY9
KOiK12831
OMAiPNAWAPP
OrthoDBiEOG091G0HAI
PhylomeDBiQ8QZY9
TreeFamiTF300890

Family and domain databases

CDDicd12334 RRM1_SF3B4, 1 hit
cd12335 RRM2_SF3B4, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034158 SF3B4_RRM1
IPR034159 SF3B4_RRM2
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8QZY9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAGPISERN QDATVYVGGL DEKVSEPLLW ELFLQAGPVV NTHMPKDRVT
60 70 80 90 100
GQHQGYGFVE FLSEEDADYA IKIMNMIKLY GKPIRVNKAS AHNKNLDVGA
110 120 130 140 150
NIFIGNLDPE IDEKLLYDTF SAFGVILQTP KIMRDPDTGN SKGYAFINFA
160 170 180 190 200
SFDASDAAIE AMNGQYLCNR PITVSYAFKK DSKGERHGSA AERLLAAQNP
210 220 230 240 250
LSQADRPHQL FADAPPPPSA PNPVVSSLGS GLPPPGMPPP GSFPPPVPPP
260 270 280 290 300
GALPPGIPPA MPPPPMPPGA GGHGPPAAGT PGAGHPGHGH SHPHPFPPGG
310 320 330 340 350
MPHPGMSQMQ LAHHGPHGLG HPHAGPPGSG GQPPPRPPPG MPHPGPPPMG
360 370 380 390 400
MPPRGPPFGS PMGHPGPMPP HGMRGPPPLM PPHGYTGPPR PPPYGYQRGP
410 420
LPPPRPTPRP PVPPRGPLRG PLPQ
Length:424
Mass (Da):44,356
Last modified:June 1, 2002 - v1
Checksum:i7B6B69A2BD95F1E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047751 mRNA Translation: BAC33145.1
AK135543 mRNA Translation: BAE22576.1
BC024418 mRNA Translation: AAH24418.3
BC026567 mRNA Translation: AAH26567.1
BC085273 mRNA Translation: AAH85273.1
CCDSiCCDS17630.1
RefSeqiNP_694693.1, NM_153053.4
UniGeneiMm.219671

Genome annotation databases

EnsembliENSMUST00000076372; ENSMUSP00000075709; ENSMUSG00000068856
GeneIDi107701
KEGGimmu:107701
UCSCiuc008qme.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047751 mRNA Translation: BAC33145.1
AK135543 mRNA Translation: BAE22576.1
BC024418 mRNA Translation: AAH24418.3
BC026567 mRNA Translation: AAH26567.1
BC085273 mRNA Translation: AAH85273.1
CCDSiCCDS17630.1
RefSeqiNP_694693.1, NM_153053.4
UniGeneiMm.219671

3D structure databases

ProteinModelPortaliQ8QZY9
SMRiQ8QZY9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223499, 2 interactors
IntActiQ8QZY9, 8 interactors
MINTiQ8QZY9
STRINGi10090.ENSMUSP00000075709

PTM databases

iPTMnetiQ8QZY9
PhosphoSitePlusiQ8QZY9

Proteomic databases

EPDiQ8QZY9
MaxQBiQ8QZY9
PaxDbiQ8QZY9
PeptideAtlasiQ8QZY9
PRIDEiQ8QZY9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076372; ENSMUSP00000075709; ENSMUSG00000068856
GeneIDi107701
KEGGimmu:107701
UCSCiuc008qme.1 mouse

Organism-specific databases

CTDi10262
MGIiMGI:109580 Sf3b4

Phylogenomic databases

eggNOGiKOG0131 Eukaryota
ENOG410XPT4 LUCA
GeneTreeiENSGT00870000136537
HOGENOMiHOG000200535
HOVERGENiHBG002295
InParanoidiQ8QZY9
KOiK12831
OMAiPNAWAPP
OrthoDBiEOG091G0HAI
PhylomeDBiQ8QZY9
TreeFamiTF300890

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway

Miscellaneous databases

PROiPR:Q8QZY9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000068856 Expressed in 33 organ(s), highest expression level in spleen
GenevisibleiQ8QZY9 MM

Family and domain databases

CDDicd12334 RRM1_SF3B4, 1 hit
cd12335 RRM2_SF3B4, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034158 SF3B4_RRM1
IPR034159 SF3B4_RRM2
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSF3B4_MOUSE
AccessioniPrimary (citable) accession number: Q8QZY9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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