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Protein

Wee1-like protein kinase 1-B

Gene

wee1-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a zygotic negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed cdk1 before the onset of mitosis by mediating phosphorylation of cdk1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed cdk1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-cdk1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric cdk1 does not occur.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei277ATPPROSITE-ProRule annotation1
Active sitei375Proton acceptorPROSITE-ProRule annotation1
Metal bindingi380Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi412Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi254 – 262ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic cell cycle Source: InterPro
  • negative regulation of cell cycle Source: CACAO

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 1-B (EC:2.7.10.2)
Alternative name(s):
Zygotic wee1-like protein kinase 1B
Short name:
Xe-Wee1B
Short name:
XeWee1B
Gene namesi
Name:wee1-b
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254033 wee1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi181 – 183NIN → AAA: Impairs without abolishing ability to phosphorylate cdk1. 1 Publication3
Mutagenesisi186T → A: Abolishes kinase activity inhibition during M-phase. Does not affect ability to phosphorylate cdk1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004095231 – 595Wee1-like protein kinase 1-BAdd BLAST595

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186Phosphothreonine; by cdk11 Publication1

Post-translational modificationi

Phosphorylation at Thr-186 during M-phase by cdk1 inhibits the kinase activity and leads to interaction with pin1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ8QGV2

PTM databases

iPTMnetiQ8QGV2

Expressioni

Tissue specificityi

Zygotically expressed. Present in oocytes and postgastrula embryos (at least until the tailbud stage). Expression begins at the midblastula stage and increases after the early gastrula stage.1 Publication

Interactioni

Subunit structurei

Interacts (when phosphorylated at Thr-186) with pin1.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi100680, 1 interactor
DIPiDIP-60878N
ELMiQ8QGV2
IntActiQ8QGV2, 3 interactors

Structurei

3D structure databases

ProteinModelPortaliQ8QGV2
SMRiQ8QGV2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini248 – 518Protein kinasePROSITE-ProRule annotationAdd BLAST271

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili526 – 563Sequence analysisAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi69 – 75Poly-Glu7

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOVERGENiHBG005050
KOiK06632

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR017164 Wee1-like_protein_kinase
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF037281 Wee1-like_protein_kinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q8QGV2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNVQPRNMNV QPRNMNVQPV RHKLFFSDTD EEEEDGHSTG EDSAFQESDS
60 70 80 90 100
PVSRQREKQE GKPPGGTWEE LEEEEGFGSS PIKSPGDFFM SDSPSYRQLA
110 120 130 140 150
PASPTRSPQG PTSPIPECPG TPPHKTFRKL RLFDTPHTPK SLLSKARGIG
160 170 180 190 200
SSALRFRGGT LFREAEKAPK PEFVYSTPQV NINPFTPDSL EIQSSAGLCR
210 220 230 240 250
GRKRALLNDS CGEDMEGSDC ELEDEDIRPA KRIPITESNM KSRYATEFHE
260 270 280 290 300
LEKIGSGEFG SVFKCVKRLD GCIYAIKRSK KPLAGSVDEQ NALREVYAHA
310 320 330 340 350
VLGQHPHVVR YYSAWAEDDH MLIQNEYCNG GSLSDVISEN YRTMQYFTEP
360 370 380 390 400
ELKDLLLQVA RGLKYIHSMS LVHMDIKPSN IFISRTTLPN TAVEEADDEE
410 420 430 440 450
CGSGKVIYKI GDLGHVTRVS SPQVEEGDSR FLANEVLQEN YTHLAKADIF
460 470 480 490 500
ALALTVWSAA GAEPFPTNGD QWHEIRQGKL PRVPQLLSQE FVDLIKLMIS
510 520 530 540 550
PDPEKRPSSV ALVKHSVLLS ASRKSAEQLR IELDAEKFKN ALLQKELKKA
560 570 580 590
QIAKAAAEER AHFPDRIATR STTQNNRTTR LIGKKMNRSV SLTIY
Length:595
Mass (Da):66,600
Last modified:June 1, 2002 - v1
Checksum:i4E90E7FDA341CB1B
GO

Sequence cautioni

The sequence AAH82404 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67T → S in AAH82404 (Ref. 2) Curated1
Sequence conflicti367H → Y in AAH82404 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071983 mRNA Translation: BAB86797.1
BC082404 mRNA Translation: AAH82404.1 Different initiation.
RefSeqiNP_001084186.1, NM_001090717.1
UniGeneiXl.9986

Genome annotation databases

GeneIDi399355
KEGGixla:399355

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071983 mRNA Translation: BAB86797.1
BC082404 mRNA Translation: AAH82404.1 Different initiation.
RefSeqiNP_001084186.1, NM_001090717.1
UniGeneiXl.9986

3D structure databases

ProteinModelPortaliQ8QGV2
SMRiQ8QGV2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi100680, 1 interactor
DIPiDIP-60878N
ELMiQ8QGV2
IntActiQ8QGV2, 3 interactors

PTM databases

iPTMnetiQ8QGV2

Proteomic databases

PRIDEiQ8QGV2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399355
KEGGixla:399355

Organism-specific databases

CTDi399355
XenbaseiXB-GENE-6254033 wee1

Phylogenomic databases

HOVERGENiHBG005050
KOiK06632

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR017164 Wee1-like_protein_kinase
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF037281 Wee1-like_protein_kinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWEE1B_XENLA
AccessioniPrimary (citable) accession number: Q8QGV2
Secondary accession number(s): Q641D3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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