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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NAD; via amide nitrogenUniRule annotation1
Binding sitei63NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi156Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi171IronUniRule annotation1
Binding sitei224NAD; via amide nitrogenUniRule annotation1
Binding sitei234GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 56NADUniRule annotation2
Nucleotide bindingi154 – 156NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayi
UPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:MM_0222
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaStenosarchaea groupMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539481 – 260Thiamine thiazole synthaseAdd BLAST260

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_0222

Structurei

3D structure databases

ProteinModelPortaliQ8Q0B5
SMRiQ8Q0B5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Q8Q0B5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELDEVIITR AIFEEYSKTF LEYTDIDVAL VGGGPANLVA AKYLAEAGAK
60 70 80 90 100
VAIYEQKLSL GGGMWAGGMM FPRIVVQEEA CRVLDDFGIR YKEYEPGYFV
110 120 130 140 150
ANSVESVGKL IAGATSAGAE VFNLVSFEDI MIRENDRVTG IVINWGPVTT
160 170 180 190 200
QRLHVDPLMI RTKLVIDGTG HDAVVCNTIL RKIPNAKIGE FGILGEKPMW
210 220 230 240 250
SEVGERLAVD ATQEIYPGLI VAGMAANAAT RAPRMGPVFG GMLLSGEKAA
260
KLALDRLKKI
Length:260
Mass (Da):28,127
Last modified:September 19, 2002 - v1
Checksum:iCBD4E09DD646AED3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA Translation: AAM29918.1
RefSeqiWP_011032176.1, NC_003901.1

Genome annotation databases

EnsemblBacteriaiAAM29918; AAM29918; MM_0222
GeneIDi24882429
KEGGimma:MM_0222
PATRICifig|192952.21.peg.270

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA Translation: AAM29918.1
RefSeqiWP_011032176.1, NC_003901.1

3D structure databases

ProteinModelPortaliQ8Q0B5
SMRiQ8Q0B5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_0222

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM29918; AAM29918; MM_0222
GeneIDi24882429
KEGGimma:MM_0222
PATRICifig|192952.21.peg.270

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Enzyme and pathway databases

UniPathwayi
UPA00060

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_METMA
AccessioniPrimary (citable) accession number: Q8Q0B5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: September 19, 2002
Last modified: October 10, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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