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Protein

Peroxiredoxin

Gene

MM_0814

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.UniRule annotation

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. Although the primary sequence of this enzyme is similar to those of the 1-Cys Prx6 enzymes, its catalytic properties resemble those of the typical 2-Cys Prxs and C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.UniRule annotation

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44Cysteine sulfenic acid (-SOH) intermediateUniRule annotation1
Binding sitei127SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Names & Taxonomyi

Protein namesi
Recommended name:
PeroxiredoxinUniRule annotation (EC:1.11.1.15UniRule annotation)
Alternative name(s):
Thioredoxin peroxidaseUniRule annotation
Gene namesi
Ordered Locus Names:MM_0814
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001351561 – 219PeroxiredoxinAdd BLAST219

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi44Interchain (with C-212); in linked formUniRule annotation
Disulfide bondi206 ↔ 212AlternateUniRule annotation
Disulfide bondi212Interchain (with C-44); in linked formUniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodecamer. Pentamer of dimers that assemble into a ring structure.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_0814

Structurei

3D structure databases

ProteinModelPortaliQ8PYP6
SMRiQ8PYP6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 164ThioredoxinUniRule annotationAdd BLAST163

Sequence similaritiesi

Belongs to the peroxiredoxin family. Prx6 subfamily.UniRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiarCOG00312 Archaea
COG0450 LUCA
HOGENOMiHOG000022346
KOiK03386
OMAiCPANWEE
OrthoDBiPOG093Z0BGG

Family and domain databases

HAMAPiMF_00401 Peroxiredoxin, 1 hit
InterProiView protein in InterPro
IPR000866 AhpC/TSA
IPR024706 Peroxiredoxin_AhpC-typ
IPR019479 Peroxiredoxin_C
IPR022915 Peroxiredoxin_TDXH
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF10417 1-cysPrx_C, 1 hit
PF00578 AhpC-TSA, 1 hit
PIRSFiPIRSF000239 AHPC, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8PYP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLIGDDAPS FTAVTTQGII KFPDDYKGKW VILFSHPADF TPVCTTEFMT
60 70 80 90 100
FASMQEEFRS MNTELIGLSI DSVFSHIAWL KRIEEKIEYK GMKNLEIKFP
110 120 130 140 150
VIEDLKMDVA KKYGMVQPKA STTQAVRAVF IIDPEAKIRT ILYYPQSTGR
160 170 180 190 200
NMQEIKRIVV ALQKNAAEKV ATPANWQPGE DVIIPPPSSM EAVKERMGKE
210
EEGKRCLDWF MCLKKDTQK
Length:219
Mass (Da):24,946
Last modified:October 10, 2002 - v1
Checksum:i064AF69A1991E812
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA Translation: AAM30510.1

Genome annotation databases

EnsemblBacteriaiAAM30510; AAM30510; MM_0814
KEGGimma:MM_0814

Similar proteinsi

Entry informationi

Entry nameiTDXH_METMA
AccessioniPrimary (citable) accession number: Q8PYP6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: October 10, 2002
Last modified: July 18, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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