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UniProtKB - Q8PXV2 (MFNB_METMA)
Protein
(5-formylfuran-3-yl)methyl phosphate synthase
Gene
mfnB
Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Functioni
Catalyzes the formation of 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate (4-HFC-P) from two molecules of glyceraldehyde-3-P (GA-3-P).
UniRule annotationCatalytic activityi
- 2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate + 2 H2O + phosphateUniRule annotationEC:4.2.3.153UniRule annotation
: methanofuran biosynthesis Pathwayi
This protein is involved in the pathway methanofuran biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway methanofuran biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 27 | Schiff-base intermediate with substrateUniRule annotation | 1 | |
Active sitei | 85 | Proton acceptorUniRule annotation | 1 |
GO - Molecular functioni
- carbon-carbon lyase activity Source: UniProtKB-UniRule
GO - Biological processi
- methanofuran biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Ligand | Schiff base |
Enzyme and pathway databases
UniPathwayi | UPA00080 |
Names & Taxonomyi
Protein namesi | Recommended name: (5-formylfuran-3-yl)methyl phosphate synthaseUniRule annotation (EC:4.2.3.153UniRule annotation)Alternative name(s): 4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthaseUniRule annotation Short name: 4-HFC-P synthaseUniRule annotation |
Gene namesi | Name:mfnBUniRule annotation Ordered Locus Names:MM_1114 |
Organismi | Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) |
Taxonomic identifieri | 192952 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Stenosarchaea group › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000134867 | 1 – 234 | (5-formylfuran-3-yl)methyl phosphate synthaseAdd BLAST | 234 |
Family & Domainsi
Sequence similaritiesi
Belongs to the MfnB family.UniRule annotation
Phylogenomic databases
eggNOGi | arCOG04482, Archaea |
HOGENOMi | CLU_068659_0_0_2 |
OMAi | NFPWVIR |
Family and domain databases
HAMAPi | MF_00681, MfnB, 1 hit |
InterProi | View protein in InterPro IPR007565, 4HFCP_synth IPR035081, 4HFCP_synth_arc IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF04476, 4HFCP_synth, 1 hit |
PIRSFi | PIRSF015957, UCP015957, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8PXV2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLLVSPINS EEAIIASIGG ADIVDVKNPK EGSLGANFPW VIREVKAVVN
60 70 80 90 100
GRQPISATIG DFNYKPGTAA LAALGAAVAG ADYIKVGLYD IQTESQALEL
110 120 130 140 150
LTKITRAVKD YNPLKKVVAS GYSDYKRINS ISPLLLPAVA AEAGVDVVMV
160 170 180 190 200
DTGVKDGKST FEFMDEKELK EFTDLAHSYG LENAIAGSLK FEDIPLLERI
210 220 230
GPDIIGVRGM VCGGDRSTSI RQELVEKLVA ECQA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE008384 Genomic DNA Translation: AAM30810.1 |
RefSeqi | WP_011033063.1, NC_003901.1 |
Genome annotation databases
EnsemblBacteriai | AAM30810; AAM30810; MM_1114 |
GeneIDi | 44085634 66137458 |
KEGGi | mma:MM_1114 |
PATRICi | fig|192952.21.peg.1303 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE008384 Genomic DNA Translation: AAM30810.1 |
RefSeqi | WP_011033063.1, NC_003901.1 |
3D structure databases
SMRi | Q8PXV2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 192952.MM_1114 |
Genome annotation databases
EnsemblBacteriai | AAM30810; AAM30810; MM_1114 |
GeneIDi | 44085634 66137458 |
KEGGi | mma:MM_1114 |
PATRICi | fig|192952.21.peg.1303 |
Phylogenomic databases
eggNOGi | arCOG04482, Archaea |
HOGENOMi | CLU_068659_0_0_2 |
OMAi | NFPWVIR |
Enzyme and pathway databases
UniPathwayi | UPA00080 |
Family and domain databases
HAMAPi | MF_00681, MfnB, 1 hit |
InterProi | View protein in InterPro IPR007565, 4HFCP_synth IPR035081, 4HFCP_synth_arc IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF04476, 4HFCP_synth, 1 hit |
PIRSFi | PIRSF015957, UCP015957, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MFNB_METMA | |
Accessioni | Q8PXV2Primary (citable) accession number: Q8PXV2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2003 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families