UniProtKB - Q8PVS9 (SPSS_METMA)
Protein
O-phospho-L-seryl-tRNA:Cys-tRNA synthase
Gene
MM_1881
Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Functioni
Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).UniRule annotation
Catalytic activityi
- H+ + hydrogen sulfide + O-phospho-L-seryl-tRNACys = L-cysteinyl-tRNACys + phosphateUniRule annotationEC:2.5.1.73UniRule annotation
Cofactori
pyridoxal 5'-phosphateUniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 90 | Sulfur donor or Sep-tRNA(Cys)UniRule annotation | 1 | |
Binding sitei | 114 | Sulfur donor or Sep-tRNA(Cys)UniRule annotation | 1 | |
Binding sitei | 115 | Sulfur donor or Sep-tRNA(Cys)UniRule annotation | 1 | |
Binding sitei | 196 | Pyridoxal phosphateUniRule annotation | 1 | |
Binding sitei | 280 | Sulfur donor or Sep-tRNA(Cys)UniRule annotation | 1 |
GO - Molecular functioni
- Sep-tRNA:Cys-tRNA synthase activity Source: UniProtKB-UniRule
GO - Biological processi
- translation Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Protein biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | MMAZ192952:G1FZI-1961-MONOMER |
BRENDAi | 2.5.1.73 3270 |
Names & Taxonomyi
Protein namesi | Recommended name: O-phospho-L-seryl-tRNA:Cys-tRNA synthaseUniRule annotation (EC:2.5.1.73UniRule annotation)Alternative name(s): Sep-tRNA:Cys-tRNA synthaseUniRule annotation Short name: SepCysSUniRule annotation |
Gene namesi | Ordered Locus Names:MM_1881 |
Organismi | Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) |
Taxonomic identifieri | 192952 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Stenosarchaea group › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000359462 | 1 – 386 | O-phospho-L-seryl-tRNA:Cys-tRNA synthaseAdd BLAST | 386 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 222 | N6-(pyridoxal phosphate)lysineUniRule annotation | 1 |
Interactioni
Subunit structurei
Homodimer.
Interacts with SepRS.
UniRule annotationProtein-protein interaction databases
STRINGi | 192952.MM_1881 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 89 – 90 | Pyridoxal phosphate bindingUniRule annotation | 2 | |
Regioni | 219 – 221 | Pyridoxal phosphate bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the SepCysS family.UniRule annotation
Phylogenomic databases
eggNOGi | arCOG00091 Archaea COG1103 LUCA |
HOGENOMi | HOG000015511 |
KOi | K06868 |
OMAi | HKSMAAS |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01675 Sep_Cys_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major IPR013375 Sep_Cys-tRNA_synth_arc IPR008829 SepSecS/SepCysS |
PANTHERi | PTHR43586:SF3 PTHR43586:SF3, 1 hit |
Pfami | View protein in Pfam PF05889 SepSecS, 1 hit |
SUPFAMi | SSF53383 SSF53383, 1 hit |
TIGRFAMsi | TIGR02539 SepCysS, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8PVS9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLDDSSLQK FGFIKRETLG SINIDPLQTG GLLTGAAKQA LVEWGDGYSV
60 70 80 90 100
CDFCGGVLDL VKKPPIHDFV HKALPEFLGC DEARVTNGAR ESKFAVMHSM
110 120 130 140 150
GKPGDWVVLD GLAHYSSYVA AERAGLNIEV VPHAGSPEYH LDPGRYGKAI
160 170 180 190 200
EEVTKENGKP PVLALVTYPD GSYGNIPDAA KIASVCHEYD VPLLLNGAYS
210 220 230 240 250
VGRMPVSAKE IGADFIVGSG HKSMAASGPV GVLGVSEEYA PVVFRKSVHN
260 270 280 290 300
KVKEIELLGC TARGATVMTL MASFPEVVKR TRNWDQEVEN ARWFSSRLEG
310 320 330 340 350
MGFIQRGQKP HSHDLMFFEA PGFYEISQKV KNGRYFLYRE LKERNIHGIK
360 370 380
SGLTKYFKLS TFGLGKEKLG TVADAFEDIL KKYENI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE008384 Genomic DNA Translation: AAM31577.1 |
RefSeqi | WP_011033816.1, NC_003901.1 |
Genome annotation databases
EnsemblBacteriai | AAM31577; AAM31577; MM_1881 |
GeneIDi | 1480223 |
KEGGi | mma:MM_1881 |
PATRICi | fig|192952.21.peg.2168 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE008384 Genomic DNA Translation: AAM31577.1 |
RefSeqi | WP_011033816.1, NC_003901.1 |
3D structure databases
SMRi | Q8PVS9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 192952.MM_1881 |
Genome annotation databases
EnsemblBacteriai | AAM31577; AAM31577; MM_1881 |
GeneIDi | 1480223 |
KEGGi | mma:MM_1881 |
PATRICi | fig|192952.21.peg.2168 |
Phylogenomic databases
eggNOGi | arCOG00091 Archaea COG1103 LUCA |
HOGENOMi | HOG000015511 |
KOi | K06868 |
OMAi | HKSMAAS |
Enzyme and pathway databases
BioCyci | MMAZ192952:G1FZI-1961-MONOMER |
BRENDAi | 2.5.1.73 3270 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01675 Sep_Cys_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major IPR013375 Sep_Cys-tRNA_synth_arc IPR008829 SepSecS/SepCysS |
PANTHERi | PTHR43586:SF3 PTHR43586:SF3, 1 hit |
Pfami | View protein in Pfam PF05889 SepSecS, 1 hit |
SUPFAMi | SSF53383 SSF53383, 1 hit |
TIGRFAMsi | TIGR02539 SepCysS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPSS_METMA | |
Accessioni | Q8PVS9Primary (citable) accession number: Q8PVS9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2009 |
Last sequence update: | October 1, 2002 | |
Last modified: | November 13, 2019 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families