UniProtKB - Q8NW68 (SYT_STAAW)
Protein
Threonine--tRNA ligase
Gene
thrS
Organism
Staphylococcus aureus (strain MW2)
Status
Functioni
Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).UniRule annotation
Catalytic activityi
- EC:6.1.1.3UniRule annotation
Cofactori
Zn2+UniRule annotation1 PublicationNote: Binds 1 zinc ion per subunit.UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 336 | ZincUniRule annotation1 Publication | 1 | |
Binding sitei | 365 | ATP1 Publication | 1 | |
Binding sitei | 365 | L-threonine1 Publication | 1 | |
Metal bindingi | 387 | Zinc; via tele nitrogenUniRule annotation1 Publication | 1 | |
Binding sitei | 468 | L-threonine1 Publication | 1 | |
Binding sitei | 471 | ATP1 Publication | 1 | |
Metal bindingi | 517 | Zinc; via pros nitrogenUniRule annotation1 Publication | 1 | |
Binding sitei | 522 | ATP1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 377 – 378 | ATP1 Publication | 2 | |
Nucleotide bindingi | 485 – 486 | ATP1 Publication | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
- threonine-tRNA ligase activity Source: UniProtKB-UniRule
- tRNA binding Source: UniProtKB-KW
GO - Biological processi
- threonyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | SAUR196620:G1G3G-1782-MONOMER |
BRENDAi | 6.1.1.3, 3352 |
Names & Taxonomyi
Protein namesi | Recommended name: Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)Alternative name(s): Threonyl-tRNA synthetaseUniRule annotation Short name: ThrRSUniRule annotation |
Gene namesi | Name:thrSUniRule annotation Ordered Locus Names:MW1626 |
Organismi | Staphylococcus aureus (strain MW2) |
Taxonomic identifieri | 196620 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101052 | 1 – 645 | Threonine--tRNA ligaseAdd BLAST | 645 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8NW68 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8NW68 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 63 | TGSPROSITE-ProRule annotationAdd BLAST | 63 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 62 | N1 domain1 PublicationAdd BLAST | 62 | |
Regioni | 63 – 223 | N2 domain1 PublicationAdd BLAST | 161 | |
Regioni | 242 – 540 | Catalytic1 PublicationAdd BLAST | 299 | |
Regioni | 541 – 645 | Anticodon recognition1 PublicationAdd BLAST | 105 |
Domaini
The protein structure shows 2 N-terminal domains, the central catalytic and C-terminal anticodon recognition domain. Comparison with the E.coli structure shows the N-terminal domains (editing region) are quite flexible with respect to the catalytic domain. The C-terminal domain recognizes the anticodon region of the tRNA while the acceptor arm is sandwiched between the N-terminal domains and the catalytic domain (PubMed:12875846).1 Publication
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_008554_0_1_9 |
OMAi | FYYDFAY |
Family and domain databases
CDDi | cd00771, ThrRS_core, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.40.50.800, 1 hit |
HAMAPi | MF_00184, Thr_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR002314, aa-tRNA-synt_IIb IPR006195, aa-tRNA-synth_II IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR012675, Beta-grasp_dom_sf IPR004095, TGS IPR012676, TGS-like IPR002320, Thr-tRNA-ligase_IIa IPR018163, Thr/Ala-tRNA-synth_IIc_edit IPR033728, ThrRS_core IPR012947, tRNA_SAD |
PANTHERi | PTHR11451, PTHR11451, 1 hit |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF02824, TGS, 1 hit PF00587, tRNA-synt_2b, 1 hit PF07973, tRNA_SAD, 1 hit |
PRINTSi | PR01047, TRNASYNTHTHR |
SMARTi | View protein in SMART SM00863, tRNA_SAD, 1 hit |
SUPFAMi | SSF55186, SSF55186, 1 hit SSF81271, SSF81271, 1 hit |
TIGRFAMsi | TIGR00418, thrS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit PS51880, TGS, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8NW68-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEQINIQFPD GNKKAFDKGT TTEDIAQSIS PGLRKKAVAG KFNGQLVDLT
60 70 80 90 100
KPLETDGSIE IVTPGSEEAL EVLRHSTAHL MAHAIKRLYG NVKFGVGPVI
110 120 130 140 150
EGGFYYDFDI DQNISSDDFE QIEKTMKQIV NENMKIERKV VSRDEAKELF
160 170 180 190 200
SNDEYKLELI DAIPEDENVT LYSQGDFTDL CRGVHVPSTA KIKEFKLLST
210 220 230 240 250
AGAYWRGDSN NKMLQRIYGT AFFDKKELKA HLQMLEERKE RDHRKIGKEL
260 270 280 290 300
ELFTNSQLVG AGLPLWLPNG ATIRREIERY IVDKEVSMGY DHVYTPVLAN
310 320 330 340 350
VDLYKTSGHW DHYQEDMFPP MQLDETESMV LRPMNCPHHM MIYANKPHSY
360 370 380 390 400
RELPIRIAEL GTMHRYEASG AVSGLQRVRG MTLNDSHIFV RPDQIKEEFK
410 420 430 440 450
RVVNMIIDVY KDFGFEDYSF RLSYRDPEDK EKYFDDDDMW NKAENMLKEA
460 470 480 490 500
ADELGLSYEE AIGEAAFYGP KLDVQVKTAM GKEETLSTAQ LDFLLPERFD
510 520 530 540 550
LTYIGQDGEH HRPVVIHRGV VSTMERFVAF LTEETKGAFP TWLAPKQVQI
560 570 580 590 600
IPVNVDLHYD YARQLQDELK SQGVRVSIDD RNEKMGYKIR EAQMQKIPYQ
610 620 630 640
IVVGDKEVEN NQVNVRQYGS QDQETVEKDE FIWNLVDEIR LKKHR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000033 Genomic DNA Translation: BAB95491.1 |
RefSeqi | WP_000435132.1, NC_003923.1 |
Genome annotation databases
EnsemblBacteriai | BAB95491; BAB95491; BAB95491 |
KEGGi | sam:MW1626 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000033 Genomic DNA Translation: BAB95491.1 |
RefSeqi | WP_000435132.1, NC_003923.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NYQ | X-ray | 3.20 | A/B | 1-645 | [»] | |
1NYR | X-ray | 2.80 | A/B | 1-645 | [»] | |
SMRi | Q8NW68 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB03355, 5'-O-(L-Threonylsulfamoyl)adenosine |
Genome annotation databases
EnsemblBacteriai | BAB95491; BAB95491; BAB95491 |
KEGGi | sam:MW1626 |
Phylogenomic databases
HOGENOMi | CLU_008554_0_1_9 |
OMAi | FYYDFAY |
Enzyme and pathway databases
BioCyci | SAUR196620:G1G3G-1782-MONOMER |
BRENDAi | 6.1.1.3, 3352 |
Miscellaneous databases
EvolutionaryTracei | Q8NW68 |
Family and domain databases
CDDi | cd00771, ThrRS_core, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.40.50.800, 1 hit |
HAMAPi | MF_00184, Thr_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR002314, aa-tRNA-synt_IIb IPR006195, aa-tRNA-synth_II IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR012675, Beta-grasp_dom_sf IPR004095, TGS IPR012676, TGS-like IPR002320, Thr-tRNA-ligase_IIa IPR018163, Thr/Ala-tRNA-synth_IIc_edit IPR033728, ThrRS_core IPR012947, tRNA_SAD |
PANTHERi | PTHR11451, PTHR11451, 1 hit |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF02824, TGS, 1 hit PF00587, tRNA-synt_2b, 1 hit PF07973, tRNA_SAD, 1 hit |
PRINTSi | PR01047, TRNASYNTHTHR |
SMARTi | View protein in SMART SM00863, tRNA_SAD, 1 hit |
SUPFAMi | SSF55186, SSF55186, 1 hit SSF81271, SSF81271, 1 hit |
TIGRFAMsi | TIGR00418, thrS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit PS51880, TGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYT_STAAW | |
Accessioni | Q8NW68Primary (citable) accession number: Q8NW68 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2003 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries