UniProtKB - Q8NUQ5 (CRTM_STAAW)
Protein
4,4'-diapophytoene synthase
Gene
crtM
Organism
Staphylococcus aureus (strain MW2)
Status
Functioni
Involved in the biosynthesis of the yellow-orange carotenoid staphyloxanthin, which plays a role in the virulence via its protective function against oxidative stress. Catalyzes the head-to-head condensation of two molecules of farnesyl diphosphate (FPP) into the colorless C(30) carotenoid 4,4'-diapophytoene (dehydrosqualene).By similarity
Catalytic activityi
- EC:2.5.1.96By similarity
Cofactori
Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity
: staphyloxanthin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes staphyloxanthin from farnesyl diphosphate.By similarityProteins known to be involved in the 5 steps of the subpathway in this organism are:
- 4,4'-diapophytoene synthase (crtM)
- 4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) (crtN)
- 4,4'-diaponeurosporene oxygenase (crtP)
- 4,4'-diaponeurosporenoate glycosyltransferase (crtQ)
- Glycosyl-4,4'-diaponeurosporenoate acyltransferase (crtO)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes staphyloxanthin from farnesyl diphosphate, the pathway staphyloxanthin biosynthesis and in Carotenoid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 41 | Farnesyl diphosphate 1By similarity | 1 | |
Binding sitei | 45 | Farnesyl diphosphate 1By similarity | 1 | |
Binding sitei | 45 | Farnesyl diphosphate 2By similarity | 1 | |
Metal bindingi | 48 | Magnesium 1By similarity | 1 | |
Metal bindingi | 52 | Magnesium 1By similarity | 1 | |
Binding sitei | 165 | Farnesyl diphosphate 2By similarity | 1 | |
Metal bindingi | 168 | Magnesium 2By similarity | 1 | |
Binding sitei | 171 | Farnesyl diphosphate 1By similarity | 1 | |
Metal bindingi | 172 | Magnesium 2By similarity | 1 | |
Binding sitei | 248 | Farnesyl diphosphate 1By similarity | 1 |
GO - Molecular functioni
- farnesyl-diphosphate farnesyltransferase activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- squalene synthase activity Source: InterPro
GO - Biological processi
- carotenoid biosynthetic process Source: UniProtKB-KW
- pathogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Carotenoid biosynthesis, Virulence |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | SAUR196620:G1G3G-2772-MONOMER |
UniPathwayi | UPA00029;UER00556 |
Names & Taxonomyi
Protein namesi | Recommended name: 4,4'-diapophytoene synthaseBy similarity (EC:2.5.1.96By similarity)Short name: DAP synthaseBy similarity Alternative name(s): C30 carotenoid synthaseBy similarity Dehydrosqualene synthaseBy similarity |
Gene namesi | Name:crtM Ordered Locus Names:MW2483 |
Organismi | Staphylococcus aureus (strain MW2) |
Taxonomic identifieri | 196620 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000282623 | 1 – 287 | 4,4'-diapophytoene synthaseAdd BLAST | 287 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 18 – 21 | Farnesyl diphosphate 1 bindingBy similarity | 4 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_037269_1_3_9 |
OMAi | KRRAIWA |
Family and domain databases
CDDi | cd00683, Trans_IPPS_HH, 1 hit |
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR002060, Squ/phyt_synthse IPR019845, Squalene/phytoene_synthase_CS IPR033904, Trans_IPPS_HH |
SFLDi | SFLDG01018, Squalene/Phytoene_Synthase_Lik, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
PROSITEi | View protein in PROSITE PS01044, SQUALEN_PHYTOEN_SYN_1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8NUQ5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTMMDMNFKY CHKIMKKHSK SFSYAFDLLP EDQRKAVWAI YAVCRKIDDS
60 70 80 90 100
IDVYGDIQFL NQIKEDIQSI EKYPYEHHHF QSDRRIMMAL QHVAQHKNIA
110 120 130 140 150
FQSFYNLIDT VYKDQHFTMF ETDAELFGYC YGVAGTVGEV LTPILSDHET
160 170 180 190 200
HQTYDVARRL GESLQLINIL RDVGEDFDNE RIYFSKQRLK QYEVDIAEVY
210 220 230 240 250
QNGVNNHYID LWEYYAAIAE KDFQDVMDQI KVFSIEAQPI IELAARIYIE
260 270 280
ILDEVRQANY TLHERVFVDK RKKAKLFHEI NSKYHRI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000033 Genomic DNA Translation: BAB96348.1 |
RefSeqi | WP_000178307.1, NC_003923.1 |
Genome annotation databases
EnsemblBacteriai | BAB96348; BAB96348; BAB96348 |
GeneIDi | 45575712 |
KEGGi | sam:MW2483 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000033 Genomic DNA Translation: BAB96348.1 |
RefSeqi | WP_000178307.1, NC_003923.1 |
3D structure databases
SMRi | Q8NUQ5 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | BAB96348; BAB96348; BAB96348 |
GeneIDi | 45575712 |
KEGGi | sam:MW2483 |
Phylogenomic databases
HOGENOMi | CLU_037269_1_3_9 |
OMAi | KRRAIWA |
Enzyme and pathway databases
UniPathwayi | UPA00029;UER00556 |
BioCyci | SAUR196620:G1G3G-2772-MONOMER |
Family and domain databases
CDDi | cd00683, Trans_IPPS_HH, 1 hit |
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR002060, Squ/phyt_synthse IPR019845, Squalene/phytoene_synthase_CS IPR033904, Trans_IPPS_HH |
SFLDi | SFLDG01018, Squalene/Phytoene_Synthase_Lik, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
PROSITEi | View protein in PROSITE PS01044, SQUALEN_PHYTOEN_SYN_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CRTM_STAAW | |
Accessioni | Q8NUQ5Primary (citable) accession number: Q8NUQ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families