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Protein

Bifunctional NAD(P)H-hydrate repair enzyme Nnr

Gene

nnr

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi65PotassiumBy similarity1
Metal bindingi123PotassiumBy similarity1
Binding sitei156NAD(P)HX (for epimerase activity)By similarity1
Metal bindingi159PotassiumBy similarity1
Binding sitei365NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity1
Binding sitei490NAD(P)HX (for dehydratase activity)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi461 – 465ADPBy similarity5
Nucleotide bindingi480 – 489ADPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciCORYNE:G18NG-10157-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
Name:nnr
Ordered Locus Names:Cgl0595, cg0688
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004164151 – 574Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST574

Interactioni

Protein-protein interaction databases

STRINGi196627.cg0688

Structurei

3D structure databases

ProteinModelPortaliQ8NSS3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 231YjeF N-terminalAdd BLAST222
Domaini277 – 568YjeF C-terminalAdd BLAST292

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 269NAD(P)H-hydrate epimeraseBy similarityAdd BLAST269
Regioni64 – 68NAD(P)HX (for epimerase activity)By similarity5
Regioni127 – 133NAD(P)HX (for epimerase activity)By similarity7
Regioni277 – 574ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST298
Regioni418 – 424NAD(P)HX (for dehydratase activity)By similarity7

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated

Phylogenomic databases

eggNOGiCOG0062 LUCA
COG0063 LUCA
HOGENOMiHOG000228405
KOiK17758
K17759
OMAiLVGPGHN

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
PROSITEiView protein in PROSITE
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q8NSS3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKPVFSVDQI RRAENTLFEL QADPDELMIS AASAVADVAL AMVDGPAPAV
60 70 80 90 100
SSEESILLLV GPGGNGGDAL YAGAFLAEEG HHVDALLLGN GKVHQSALAY
110 120 130 140 150
YESLGGQIIS DFPPHYLYRL VIDGLFGIGG RGGLTPELAS LVESFSASGI
160 170 180 190 200
PILAIDVPSG VHADSGELPP GVMVTVEGFD NDAPMARQKI PAHIDADVTI
210 220 230 240 250
TFGGLRRAHA VSPACGEVLC ADINIAGGGG KSLSAELSQV QAEDATPQMF
260 270 280 290 300
ASKAYQRKDS LFERANLKAT APHIHRIGQH FTVLNMEPGP DHDKYSGGIV
310 320 330 340 350
GIVAGSGTYP GAAVLSVKAA VRATSAMVRY VGPALNFVIQ SLPEVVATQS
360 370 380 390 400
LATAGRVQAW VHGPGRGLEA EQSAELAELL SRPEPVLIDA DSLSLLQLSA
410 420 430 440 450
ELRQALRERK APTVLTPHKG EFERIAAELR SEGVEIPQAD KDPIGAAQAL
460 470 480 490 500
AKEFDCCVLL KGKYTVIAAH DFVHAINAGH SWLATPGSGD VLSGLVGAHL
510 520 530 540 550
AQSYAELNRL PEFFPDVTLS DSAIYTQIAP AATIHAVAAG LAARTEFGFA
560 570
PTSASLIADA IPAATAKVDL KRIV
Length:574
Mass (Da):59,796
Last modified:October 1, 2002 - v1
Checksum:i23A7F967ED25B1D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA Translation: BAB97988.1
BX927149 Genomic DNA Translation: CAF19300.1
RefSeqiNP_599831.1, NC_003450.3
WP_011013753.1, NC_006958.1

Genome annotation databases

EnsemblBacteriaiBAB97988; BAB97988; BAB97988
CAF19300; CAF19300; cg0688
GeneIDi1018599
KEGGicgb:cg0688
cgl:NCgl0570
PATRICifig|196627.13.peg.586

Similar proteinsi

Entry informationi

Entry nameiNNR_CORGL
AccessioniPrimary (citable) accession number: Q8NSS3
Secondary accession number(s): Q6M7G8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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