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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATP; via amide nitrogenUniRule annotation1
Metal bindingi107Magnesium; catalyticUniRule annotation1
Sitei108Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei131Proton acceptorUniRule annotation1
Binding sitei166Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei226SubstrateUniRule annotation1
Binding sitei270Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi76 – 77ATPUniRule annotation2
Nucleotide bindingi106 – 109ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCORYNE:G18NG-10823-MONOMER
UniPathwayi
UPA00109;UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Cgl1250, cg1409
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119481 – 346ATP-dependent 6-phosphofructokinaseAdd BLAST346

Proteomic databases

PRIDEiQ8NR14

2D gel databases

World-2DPAGEi0001:Q8NR14

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.NCgl1202

Structurei

3D structure databases

ProteinModelPortaliQ8NR14
SMRiQ8NR14
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 131Substrate bindingUniRule annotation3
Regioni173 – 175Substrate bindingUniRule annotation3
Regioni276 – 279Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
KOiK21071
OMAiGKLHSII

Family and domain databases

HAMAPiMF_01976 Phosphofructokinase_III, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR012829 Phosphofructokinase_III
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02483 PFK_mixed, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8NR14-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEDMRIATLT SGGDCPGLNA VIRGIVRTAS NEFGSTVVGY QDGWEGLLGD
60 70 80 90 100
RRVQLYDDED IDRILLRGGT ILGTGRLHPD KFKAGIDQIK ANLEDAGIDA
110 120 130 140 150
LIPIGGEGTL KGAKWLSDNG IPVVGVPKTI DNDVNGTDFT FGFDTAVAVA
160 170 180 190 200
TDAVDRLHTT AESHNRVMIV EVMGRHVGWI ALHAGMAGGA HYTVIPEVPF
210 220 230 240 250
DIAEICKAME RRFQMGEKYG IIVVAEGALP REGTMELREG HIDQFGHKTF
260 270 280 290 300
TGIGQQIADE IHVRLGHDVR TTVLGHIQRG GTPTAFDRVL ATRYGVRAAR
310 320 330 340
ACHEGSFDKV VALKGESIEM ITFEEAVGTL KEVPFERWVT AQAMFG
Length:346
Mass (Da):37,464
Last modified:October 19, 2002 - v1
Checksum:i96713869752962B0
GO

Sequence cautioni

The sequence CAF19953 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA Translation: BAB98643.1
BX927151 Genomic DNA Translation: CAF19953.1 Different initiation.
RefSeqiNP_600473.1, NC_003450.3
WP_011014231.1, NC_006958.1

Genome annotation databases

EnsemblBacteriaiBAB98643; BAB98643; BAB98643
CAF19953; CAF19953; cg1409
GeneIDi1019232
KEGGicgb:cg1409
cgl:NCgl1202
PATRICifig|196627.13.peg.1227

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA Translation: BAB98643.1
BX927151 Genomic DNA Translation: CAF19953.1 Different initiation.
RefSeqiNP_600473.1, NC_003450.3
WP_011014231.1, NC_006958.1

3D structure databases

ProteinModelPortaliQ8NR14
SMRiQ8NR14
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.NCgl1202

2D gel databases

World-2DPAGEi0001:Q8NR14

Proteomic databases

PRIDEiQ8NR14

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB98643; BAB98643; BAB98643
CAF19953; CAF19953; cg1409
GeneIDi1019232
KEGGicgb:cg1409
cgl:NCgl1202
PATRICifig|196627.13.peg.1227

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
KOiK21071
OMAiGKLHSII

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

BioCyciCORYNE:G18NG-10823-MONOMER

Family and domain databases

HAMAPiMF_01976 Phosphofructokinase_III, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR012829 Phosphofructokinase_III
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02483 PFK_mixed, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_CORGL
AccessioniPrimary (citable) accession number: Q8NR14
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: October 10, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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