UniProtKB - Q8NHV4 (NEDD1_HUMAN)
Protein
Protein NEDD1
Gene
NEDD1
Organism
Homo sapiens (Human)
Status
Functioni
Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.2 Publications
GO - Biological processi
- cell division Source: UniProtKB-KW
- ciliary basal body-plasma membrane docking Source: Reactome
- G2/M transition of mitotic cell cycle Source: Reactome
- protein localization to centrosome Source: GO_Central
- regulation of G2/M transition of mitotic cell cycle Source: Reactome
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q8NHV4 |
Reactomei | R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-380259, Loss of Nlp from mitotic centrosomes R-HSA-380270, Recruitment of mitotic centrosome proteins and complexes R-HSA-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-380320, Recruitment of NuMA to mitotic centrosomes R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-8854518, AURKA Activation by TPX2 |
SignaLinki | Q8NHV4 |
SIGNORi | Q8NHV4 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein NEDD1Alternative name(s): Neural precursor cell expressed developmentally down-regulated protein 1 Short name: NEDD-1 |
Gene namesi | Name:NEDD1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000139350.11 |
HGNCi | HGNC:7723, NEDD1 |
MIMi | 600372, gene |
neXtProti | NX_Q8NHV4 |
Subcellular locationi
Cytoskeleton
- centrosome 3 Publications
Cytoskeleton
- centriole Source: GO_Central
- centrosome Source: UniProtKB
- ciliary basal body Source: GO_Central
- pericentriolar material Source: GO_Central
- spindle pole Source: GO_Central
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- apical part of cell Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Organism-specific databases
DisGeNETi | 121441 |
OpenTargetsi | ENSG00000139350 |
PharmGKBi | PA31531 |
Miscellaneous databases
Pharosi | Q8NHV4, Tbio |
Polymorphism and mutation databases
BioMutai | NEDD1 |
DMDMi | 74762597 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051095 | 1 – 660 | Protein NEDD1Add BLAST | 660 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 325 | PhosphoserineCombined sources | 1 | |
Modified residuei | 382 | Phosphothreonine; by PLK11 Publication | 1 | |
Modified residuei | 397 | Phosphoserine; by PLK1Combined sources1 Publication | 1 | |
Modified residuei | 411 | PhosphoserineCombined sources | 1 | |
Modified residuei | 426 | Phosphoserine; by PLK11 Publication | 1 | |
Modified residuei | 468 | PhosphoserineCombined sources | 1 | |
Modified residuei | 516 | PhosphoserineCombined sources | 1 | |
Modified residuei | 550 | Phosphothreonine; by CDK11 Publication | 1 | |
Modified residuei | 637 | Phosphoserine; by PLK11 Publication | 1 |
Post-translational modificationi
During mitosis, prior phosphorylation on Thr-550 by CDK1 promotes subsequent phosphorylation by PLK1 on Thr-382, Ser-397, Ser-426 and Ser-637. Phosphorylated NEDD1 can interact with gamma-tubulin for targeting the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8NHV4 |
jPOSTi | Q8NHV4 |
MassIVEi | Q8NHV4 |
MaxQBi | Q8NHV4 |
PaxDbi | Q8NHV4 |
PeptideAtlasi | Q8NHV4 |
PRIDEi | Q8NHV4 |
ProteomicsDBi | 32921 73763 [Q8NHV4-1] 73764 [Q8NHV4-2] |
PTM databases
iPTMneti | Q8NHV4 |
PhosphoSitePlusi | Q8NHV4 |
Expressioni
Gene expression databases
Bgeei | ENSG00000139350, Expressed in secondary oocyte and 209 other tissues |
ExpressionAtlasi | Q8NHV4, baseline and differential |
Genevisiblei | Q8NHV4, HS |
Organism-specific databases
HPAi | ENSG00000139350, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Q8NHV4
With | #Exp. | IntAct |
---|---|---|
HAUS6 [Q7Z4H7] | 2 | EBI-2555055,EBI-2558196 |
Protein-protein interaction databases
BioGRIDi | 125728, 166 interactors |
DIPi | DIP-48838N |
IntActi | Q8NHV4, 101 interactors |
STRINGi | 9606.ENSP00000451211 |
Miscellaneous databases
RNActi | Q8NHV4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 1 – 31 | WD 1Add BLAST | 31 | |
Repeati | 32 – 71 | WD 2Add BLAST | 40 | |
Repeati | 75 – 114 | WD 3Add BLAST | 40 | |
Repeati | 117 – 156 | WD 4Add BLAST | 40 | |
Repeati | 160 – 200 | WD 5Add BLAST | 41 | |
Repeati | 204 – 244 | WD 6Add BLAST | 41 | |
Repeati | 246 – 285 | WD 7Add BLAST | 40 | |
Repeati | 289 – 332 | WD 8Add BLAST | 44 |
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG4378, Eukaryota |
GeneTreei | ENSGT00390000001561 |
HOGENOMi | CLU_415014_0_0_1 |
InParanoidi | Q8NHV4 |
OMAi | RHLKYSS |
PhylomeDBi | Q8NHV4 |
TreeFami | TF329816 |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NHV4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQENLRFASS GDDIKIWDAS SMTLVDKFNP HTSPHGISSI CWSSNNNFLV
60 70 80 90 100
TASSSGDKIV VSSCKCKPVP LLELAEGQKQ TCVNLNSTSM YLVSGGLNNT
110 120 130 140 150
VNIWDLKSKR VHRSLKDHKD QVTCVTYNWN DCYIASGSLS GEIILHSVTT
160 170 180 190 200
NLSSTPFGHG SNQSVRHLKY SLFKKSLLGS VSDNGIVTLW DVNSQSPYHN
210 220 230 240 250
FDSVHKAPAS GICFSPVNEL LFVTIGLDKR IILYDTSSKK LVKTLVADTP
260 270 280 290 300
LTAVDFMPDG ATLAIGSSRG KIYQYDLRML KSPVKTISAH KTSVQCIAFQ
310 320 330 340 350
YSTVLTKSSL NKGCSNKPTT VNKRSVNVNA ASGGVQNSGI VREAPATSIA
360 370 380 390 400
TVLPQPMTSA MGKGTVAVQE KAGLPRSINT DTLSKETDSG KNQDFSSFDD
410 420 430 440 450
TGKSSLGDMF SPIRDDAVVN KGSDESIGKG DGFDFLPQLN SVFPPRKNPV
460 470 480 490 500
TSSTSVLHSS PLNVFMGSPG KEENENRDLT AESKKIYMGK QESKDSFKQL
510 520 530 540 550
AKLVTSGAES GNLNTSPSSN QTRNSEKFEK PENEIEAQLI CEPPINGSST
560 570 580 590 600
PNPKIASSVT AGVASSLSEK IADSIGNNRQ NAPLTSIQIR FIQNMIQETL
610 620 630 640 650
DDFREACHRD IVNLQVEMIK QFHMQLNEMH SLLERYSVNE GLVAEIERLR
660
EENKRLRAHF
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V2V3 | G3V2V3_HUMAN | Protein NEDD1 | NEDD1 | 167 | Annotation score: | ||
G3V4I9 | G3V4I9_HUMAN | Protein NEDD1 | NEDD1 | 160 | Annotation score: | ||
G3V4L2 | G3V4L2_HUMAN | Protein NEDD1 | NEDD1 | 164 | Annotation score: | ||
G3V2S2 | G3V2S2_HUMAN | Protein NEDD1 | NEDD1 | 81 | Annotation score: | ||
G3V2M9 | G3V2M9_HUMAN | Protein NEDD1 | NEDD1 | 100 | Annotation score: |
Sequence cautioni
The sequence AAH27605 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 170 | Y → C in BAC04099 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 409 | M → V in BAC04099 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 649 | L → P in BAC04099 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_043411 | 1 – 89 | Missing in isoform 2. 1 PublicationAdd BLAST | 89 | |
Alternative sequenceiVSP_053794 | 1 | M → MHFTGAVM in isoform 3. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK093221 mRNA Translation: BAC04099.1 AK303656 mRNA Translation: BAG64657.1 AK315821 mRNA Translation: BAF98712.1 AC007564 Genomic DNA No translation available. AC013417 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW97577.1 CH471054 Genomic DNA Translation: EAW97579.1 CH471054 Genomic DNA Translation: EAW97580.1 BC027605 mRNA Translation: AAH27605.1 Different initiation. |
CCDSi | CCDS44955.1 [Q8NHV4-3] CCDS44956.1 [Q8NHV4-2] CCDS9063.1 [Q8NHV4-1] |
RefSeqi | NP_001128647.1, NM_001135175.1 [Q8NHV4-3] NP_001128648.1, NM_001135176.1 [Q8NHV4-1] NP_001128649.1, NM_001135177.1 [Q8NHV4-2] NP_690869.1, NM_152905.3 [Q8NHV4-1] XP_005268701.1, XM_005268644.2 [Q8NHV4-3] XP_006719300.1, XM_006719237.3 [Q8NHV4-2] XP_011536205.1, XM_011537903.2 [Q8NHV4-2] |
Genome annotation databases
Ensembli | ENST00000266742; ENSP00000266742; ENSG00000139350 [Q8NHV4-1] ENST00000411739; ENSP00000411307; ENSG00000139350 [Q8NHV4-2] ENST00000429527; ENSP00000404978; ENSG00000139350 [Q8NHV4-1] ENST00000457368; ENSP00000407964; ENSG00000139350 [Q8NHV4-2] ENST00000557644; ENSP00000451211; ENSG00000139350 [Q8NHV4-3] |
GeneIDi | 121441 |
KEGGi | hsa:121441 |
UCSCi | uc001teu.5, human [Q8NHV4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK093221 mRNA Translation: BAC04099.1 AK303656 mRNA Translation: BAG64657.1 AK315821 mRNA Translation: BAF98712.1 AC007564 Genomic DNA No translation available. AC013417 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW97577.1 CH471054 Genomic DNA Translation: EAW97579.1 CH471054 Genomic DNA Translation: EAW97580.1 BC027605 mRNA Translation: AAH27605.1 Different initiation. |
CCDSi | CCDS44955.1 [Q8NHV4-3] CCDS44956.1 [Q8NHV4-2] CCDS9063.1 [Q8NHV4-1] |
RefSeqi | NP_001128647.1, NM_001135175.1 [Q8NHV4-3] NP_001128648.1, NM_001135176.1 [Q8NHV4-1] NP_001128649.1, NM_001135177.1 [Q8NHV4-2] NP_690869.1, NM_152905.3 [Q8NHV4-1] XP_005268701.1, XM_005268644.2 [Q8NHV4-3] XP_006719300.1, XM_006719237.3 [Q8NHV4-2] XP_011536205.1, XM_011537903.2 [Q8NHV4-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 125728, 166 interactors |
DIPi | DIP-48838N |
IntActi | Q8NHV4, 101 interactors |
STRINGi | 9606.ENSP00000451211 |
PTM databases
iPTMneti | Q8NHV4 |
PhosphoSitePlusi | Q8NHV4 |
Polymorphism and mutation databases
BioMutai | NEDD1 |
DMDMi | 74762597 |
Proteomic databases
EPDi | Q8NHV4 |
jPOSTi | Q8NHV4 |
MassIVEi | Q8NHV4 |
MaxQBi | Q8NHV4 |
PaxDbi | Q8NHV4 |
PeptideAtlasi | Q8NHV4 |
PRIDEi | Q8NHV4 |
ProteomicsDBi | 32921 73763 [Q8NHV4-1] 73764 [Q8NHV4-2] |
Protocols and materials databases
Antibodypediai | 30194, 206 antibodies |
DNASUi | 121441 |
Genome annotation databases
Ensembli | ENST00000266742; ENSP00000266742; ENSG00000139350 [Q8NHV4-1] ENST00000411739; ENSP00000411307; ENSG00000139350 [Q8NHV4-2] ENST00000429527; ENSP00000404978; ENSG00000139350 [Q8NHV4-1] ENST00000457368; ENSP00000407964; ENSG00000139350 [Q8NHV4-2] ENST00000557644; ENSP00000451211; ENSG00000139350 [Q8NHV4-3] |
GeneIDi | 121441 |
KEGGi | hsa:121441 |
UCSCi | uc001teu.5, human [Q8NHV4-1] |
Organism-specific databases
CTDi | 121441 |
DisGeNETi | 121441 |
EuPathDBi | HostDB:ENSG00000139350.11 |
GeneCardsi | NEDD1 |
HGNCi | HGNC:7723, NEDD1 |
HPAi | ENSG00000139350, Low tissue specificity |
MIMi | 600372, gene |
neXtProti | NX_Q8NHV4 |
OpenTargetsi | ENSG00000139350 |
PharmGKBi | PA31531 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4378, Eukaryota |
GeneTreei | ENSGT00390000001561 |
HOGENOMi | CLU_415014_0_0_1 |
InParanoidi | Q8NHV4 |
OMAi | RHLKYSS |
PhylomeDBi | Q8NHV4 |
TreeFami | TF329816 |
Enzyme and pathway databases
PathwayCommonsi | Q8NHV4 |
Reactomei | R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-380259, Loss of Nlp from mitotic centrosomes R-HSA-380270, Recruitment of mitotic centrosome proteins and complexes R-HSA-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-380320, Recruitment of NuMA to mitotic centrosomes R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-8854518, AURKA Activation by TPX2 |
SignaLinki | Q8NHV4 |
SIGNORi | Q8NHV4 |
Miscellaneous databases
BioGRID-ORCSi | 121441, 733 hits in 852 CRISPR screens |
ChiTaRSi | NEDD1, human |
GeneWikii | NEDD1 |
GenomeRNAii | 121441 |
Pharosi | Q8NHV4, Tbio |
PROi | PR:Q8NHV4 |
RNActi | Q8NHV4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000139350, Expressed in secondary oocyte and 209 other tissues |
ExpressionAtlasi | Q8NHV4, baseline and differential |
Genevisiblei | Q8NHV4, HS |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NEDD1_HUMAN | |
Accessioni | Q8NHV4Primary (citable) accession number: Q8NHV4 Secondary accession number(s): B0AZN0 Q8NA30 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM