UniProtKB - Q8NHJ6 (LIRB4_HUMAN)
Protein
Leukocyte immunoglobulin-like receptor subfamily B member 4
Gene
LILRB4
Organism
Homo sapiens (Human)
Status
Functioni
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.2 Publications
GO - Molecular functioni
- antigen binding Source: ProtInc
- signaling receptor activity Source: ProtInc
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- negative regulation of osteoclast differentiation Source: UniProtKB
- negative regulation of pri-miRNA transcription by RNA polymerase II Source: BHF-UCL
- negative regulation of protein localization to nucleus Source: BHF-UCL
- negative regulation of T cell receptor signaling pathway Source: BHF-UCL
- positive regulation of regulatory T cell differentiation Source: BHF-UCL
- signal transduction Source: ProtInc
Keywordsi
Molecular function | Receptor |
Biological process | Adaptive immunity, Immunity |
Enzyme and pathway databases
PathwayCommonsi | Q8NHJ6 |
Reactomei | R-HSA-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell |
SIGNORi | Q8NHJ6 |
Names & Taxonomyi
Protein namesi | Recommended name: Leukocyte immunoglobulin-like receptor subfamily B member 4Alternative name(s): CD85 antigen-like family member K Immunoglobulin-like transcript 3 Short name: ILT-3 Leukocyte immunoglobulin-like receptor 5 Short name: LIR-5 Monocyte inhibitory receptor HM18 CD_antigen: CD85k |
Gene namesi | Name:LILRB4 Synonyms:ILT3, LIR5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000186818.12 |
HGNCi | HGNC:6608, LILRB4 |
MIMi | 604821, gene |
neXtProti | NX_Q8NHJ6 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Note: Ligand binding leads to internalization and translocation to an antigen-processing compartment.
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: ProtInc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 259 | ExtracellularSequence analysisAdd BLAST | 238 | |
Transmembranei | 260 – 280 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 281 – 448 | CytoplasmicSequence analysisAdd BLAST | 168 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 11006 |
PharmGKBi | PA30382 |
Miscellaneous databases
Pharosi | Q8NHJ6, Tbio |
Polymorphism and mutation databases
BioMutai | LILRB4 |
DMDMi | 322510117 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000014823 | 22 – 448 | Leukocyte immunoglobulin-like receptor subfamily B member 4Add BLAST | 427 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 49 ↔ 98 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 144 ↔ 195 | PROSITE-ProRule annotation1 Publication | ||
Modified residuei | 319 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
jPOSTi | Q8NHJ6 |
MassIVEi | Q8NHJ6 |
PaxDbi | Q8NHJ6 |
PeptideAtlasi | Q8NHJ6 |
PRIDEi | Q8NHJ6 |
ProteomicsDBi | 73714 [Q8NHJ6-1] 73715 [Q8NHJ6-2] 73716 [Q8NHJ6-3] |
PTM databases
GlyGeni | Q8NHJ6, 2 sites, 3 O-linked glycans (2 sites) |
iPTMneti | Q8NHJ6 |
PhosphoSitePlusi | Q8NHJ6 |
Expressioni
Tissue specificityi
Detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells.3 Publications
Inductioni
Upon contact with CD8+CD28- alloantigen-specific T suppressor (Ts) cells.
Gene expression databases
Bgeei | ENSG00000186818, Expressed in layer of synovial tissue and 167 other tissues |
ExpressionAtlasi | Q8NHJ6, baseline and differential |
Genevisiblei | Q8NHJ6, HS |
Organism-specific databases
HPAi | ENSG00000186818, Tissue enhanced (blood, lymphoid tissue) |
Interactioni
Subunit structurei
Binds PTPN6 when phosphorylated.
Binary interactionsi
Q8NHJ6
With | #Exp. | IntAct |
---|---|---|
PTPN6 [P29350] | 4 | EBI-2805248,EBI-78260 |
Protein-protein interaction databases
BioGRIDi | 116197, 16 interactors |
IntActi | Q8NHJ6, 15 interactors |
STRINGi | 9606.ENSP00000375616 |
Miscellaneous databases
RNActi | Q8NHJ6, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8NHJ6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 118 | Ig-like C2-type 1Add BLAST | 92 | |
Domaini | 124 – 218 | Ig-like C2-type 2Add BLAST | 95 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 358 – 363 | ITIM motif 1 | 6 | |
Motifi | 410 – 415 | ITIM motif 2 | 6 | |
Motifi | 440 – 445 | ITIM motif 3 | 6 |
Domaini
Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RU0A, Eukaryota |
InParanoidi | Q8NHJ6 |
OrthoDBi | 1000446at2759 |
PhylomeDBi | Q8NHJ6 |
TreeFami | TF336644 |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013151, Immunoglobulin |
Pfami | View protein in Pfam PF00047, ig, 1 hit PF13895, Ig_2, 1 hit |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 25 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NHJ6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIPTFTALLC LGLSLGPRTH MQAGPLPKPT LWAEPGSVIS WGNSVTIWCQ
60 70 80 90 100
GTLEAREYRL DKEESPAPWD RQNPLEPKNK ARFSIPSMTE DYAGRYRCYY
110 120 130 140 150
RSPVGWSQPS DPLELVMTGA YSKPTLSALP SPLVTSGKSV TLLCQSRSPM
160 170 180 190 200
DTFLLIKERA AHPLLHLRSE HGAQQHQAEF PMSPVTSVHG GTYRCFSSHG
210 220 230 240 250
FSHYLLSHPS DPLELIVSGS LEDPRPSPTR SVSTAAGPED QPLMPTGSVP
260 270 280 290 300
HSGLRRHWEV LIGVLVVSIL LLSLLLFLLL QHWRQGKHRT LAQRQADFQR
310 320 330 340 350
PPGAAEPEPK DGGLQRRSSP AADVQGENFC AAVKNTQPED GVEMDTRQSP
360 370 380 390 400
HDEDPQAVTY AKVKHSRPRR EMASPPSPLS GEFLDTKDRQ AEEDRQMDTE
410 420 430 440
AAASEAPQDV TYAQLHSFTL RQKATEPPPS QEGASPAEPS VYATLAIH
Computationally mapped potential isoform sequencesi
There are 25 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MSZ8 | A0A0A0MSZ8_HUMAN | Leukocyte immunoglobulin-like recep... | LILRB4 | 447 | Annotation score: | ||
C9JST2 | C9JST2_HUMAN | Leukocyte immunoglobulin-like recep... | LILRB4 | 399 | Annotation score: | ||
A0A0A0MS20 | A0A0A0MS20_HUMAN | Leukocyte immunoglobulin-like recep... | LILRB4 | 449 | Annotation score: | ||
A0A0G2JM38 | A0A0G2JM38_HUMAN | Leukocyte immunoglobulin-like recep... | LILRB4 | 437 | Annotation score: | ||
A0A0G2JM43 | A0A0G2JM43_HUMAN | Leukocyte immunoglobulin-like recep... | LILRB4 | 399 | Annotation score: | ||
A0A0G2JM57 | A0A0G2JM57_HUMAN | Leukocyte immunoglobulin-like recep... | LILRB4 | 447 | Annotation score: | ||
A0A0G2JM84 | A0A0G2JM84_HUMAN | Leukocyte immunoglobulin-like recep... | LILRB4 | 448 | Annotation score: | ||
A0A0G2JMH7 | A0A0G2JMH7_HUMAN | Leukocyte immunoglobulin-like recep... | LILRB4 | 449 | Annotation score: | ||
A0A0G2JNE9 | A0A0G2JNE9_HUMAN | Leukocyte immunoglobulin-like recep... | LILRB4 | 448 | Annotation score: | ||
A0A0G2JP25 | A0A0G2JP25_HUMAN | Leukocyte immunoglobulin-like recep... | LILRB4 | 448 | Annotation score: | ||
There are more potential isoformsShow all |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_025501 | 5 | F → L1 PublicationCorresponds to variant dbSNP:rs28366008Ensembl. | 1 | |
Natural variantiVAR_025502 | 18 | R → S. Corresponds to variant dbSNP:rs11540761Ensembl. | 1 | |
Natural variantiVAR_047846 | 20 | H → D1 PublicationCorresponds to variant dbSNP:rs11540762Ensembl. | 1 | |
Natural variantiVAR_017014 | 223 | D → G4 PublicationsCorresponds to variant dbSNP:rs731170Ensembl. | 1 | |
Natural variantiVAR_025503 | 330 | C → Y. Corresponds to variant dbSNP:rs11574575Ensembl. | 1 | |
Natural variantiVAR_025504 | 335 | N → D. Corresponds to variant dbSNP:rs11574576Ensembl. | 1 | |
Natural variantiVAR_017015 | 362 | K → E1 PublicationCorresponds to variant dbSNP:rs2764337Ensembl. | 1 | |
Natural variantiVAR_030939 | 362 | K → T. Corresponds to variant dbSNP:rs11574589Ensembl. | 1 | |
Natural variantiVAR_025505 | 414 | Q → R2 PublicationsCorresponds to variant dbSNP:rs1048801Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_035939 | 330 | C → SG in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_008460 | 348 | Missing in isoform 2. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U91925 mRNA Translation: AAB68665.1 AF025532 mRNA Translation: AAB87666.1 AF189768 Genomic DNA Translation: AAG02024.1 AF283988 mRNA Translation: AAL36992.1 CR542048 mRNA Translation: CAG46845.1 CR609786 mRNA No translation available. AC009892 Genomic DNA No translation available. AC011515 Genomic DNA No translation available. BC026309 mRNA Translation: AAH26309.1 |
CCDSi | CCDS12902.1 [Q8NHJ6-1] CCDS42618.1 [Q8NHJ6-2] |
RefSeqi | NP_001265355.2, NM_001278426.3 NP_001265356.2, NM_001278427.3 NP_001265357.2, NM_001278428.3 NP_001265358.2, NM_001278429.3 NP_001265359.2, NM_001278430.3 |
Genome annotation databases
Ensembli | ENST00000391736; ENSP00000375616; ENSG00000186818 ENST00000612454; ENSP00000479829; ENSG00000275730 [Q8NHJ6-1] ENST00000614699; ENSP00000478542; ENSG00000275730 [Q8NHJ6-3] ENST00000621693; ENSP00000482234; ENSG00000275730 [Q8NHJ6-2] |
GeneIDi | 11006 |
KEGGi | hsa:11006 |
UCSCi | uc002qgp.5, human [Q8NHJ6-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U91925 mRNA Translation: AAB68665.1 AF025532 mRNA Translation: AAB87666.1 AF189768 Genomic DNA Translation: AAG02024.1 AF283988 mRNA Translation: AAL36992.1 CR542048 mRNA Translation: CAG46845.1 CR609786 mRNA No translation available. AC009892 Genomic DNA No translation available. AC011515 Genomic DNA No translation available. BC026309 mRNA Translation: AAH26309.1 |
CCDSi | CCDS12902.1 [Q8NHJ6-1] CCDS42618.1 [Q8NHJ6-2] |
RefSeqi | NP_001265355.2, NM_001278426.3 NP_001265356.2, NM_001278427.3 NP_001265357.2, NM_001278428.3 NP_001265358.2, NM_001278429.3 NP_001265359.2, NM_001278430.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3P2T | X-ray | 1.70 | A | 24-219 | [»] | |
6K7O | X-ray | 3.00 | A/P/Q/R | 24-118 | [»] | |
SMRi | Q8NHJ6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116197, 16 interactors |
IntActi | Q8NHJ6, 15 interactors |
STRINGi | 9606.ENSP00000375616 |
PTM databases
GlyGeni | Q8NHJ6, 2 sites, 3 O-linked glycans (2 sites) |
iPTMneti | Q8NHJ6 |
PhosphoSitePlusi | Q8NHJ6 |
Polymorphism and mutation databases
BioMutai | LILRB4 |
DMDMi | 322510117 |
Proteomic databases
jPOSTi | Q8NHJ6 |
MassIVEi | Q8NHJ6 |
PaxDbi | Q8NHJ6 |
PeptideAtlasi | Q8NHJ6 |
PRIDEi | Q8NHJ6 |
ProteomicsDBi | 73714 [Q8NHJ6-1] 73715 [Q8NHJ6-2] 73716 [Q8NHJ6-3] |
Protocols and materials databases
Antibodypediai | 35096, 281 antibodies |
DNASUi | 11006 |
Genome annotation databases
Ensembli | ENST00000391736; ENSP00000375616; ENSG00000186818 ENST00000612454; ENSP00000479829; ENSG00000275730 [Q8NHJ6-1] ENST00000614699; ENSP00000478542; ENSG00000275730 [Q8NHJ6-3] ENST00000621693; ENSP00000482234; ENSG00000275730 [Q8NHJ6-2] |
GeneIDi | 11006 |
KEGGi | hsa:11006 |
UCSCi | uc002qgp.5, human [Q8NHJ6-1] |
Organism-specific databases
CTDi | 11006 |
DisGeNETi | 11006 |
EuPathDBi | HostDB:ENSG00000186818.12 |
GeneCardsi | LILRB4 |
HGNCi | HGNC:6608, LILRB4 |
HPAi | ENSG00000186818, Tissue enhanced (blood, lymphoid tissue) |
MIMi | 604821, gene |
neXtProti | NX_Q8NHJ6 |
PharmGKBi | PA30382 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502RU0A, Eukaryota |
InParanoidi | Q8NHJ6 |
OrthoDBi | 1000446at2759 |
PhylomeDBi | Q8NHJ6 |
TreeFami | TF336644 |
Enzyme and pathway databases
PathwayCommonsi | Q8NHJ6 |
Reactomei | R-HSA-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell |
SIGNORi | Q8NHJ6 |
Miscellaneous databases
BioGRID-ORCSi | 11006, 3 hits in 838 CRISPR screens |
ChiTaRSi | LILRB4, human |
GeneWikii | LILRB4 |
GenomeRNAii | 11006 |
Pharosi | Q8NHJ6, Tbio |
PROi | PR:Q8NHJ6 |
RNActi | Q8NHJ6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000186818, Expressed in layer of synovial tissue and 167 other tissues |
ExpressionAtlasi | Q8NHJ6, baseline and differential |
Genevisiblei | Q8NHJ6, HS |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013151, Immunoglobulin |
Pfami | View protein in Pfam PF00047, ig, 1 hit PF13895, Ig_2, 1 hit |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LIRB4_HUMAN | |
Accessioni | Q8NHJ6Primary (citable) accession number: Q8NHJ6 Secondary accession number(s): A8MVL8 Q8NHL5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2003 |
Last sequence update: | February 8, 2011 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries