UniProtKB - Q8NG68 (TTL_HUMAN)
Protein
Tubulin--tyrosine ligase
Gene
TTL
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.1 Publication
Catalytic activityi
- ATP + C-terminal L-α-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + L-tyrosine = ADP + C-terminal L-α-aminoacyl-L-glutamyl-L-glutamyl-L-tyrosyl-[tubulin] + H+ + phosphateBy similarityEC:6.3.2.25By similarity
Cofactori
Protein has several cofactor binding sites:GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- tubulin-tyrosine ligase activity Source: UniProtKB
GO - Biological processi
- C-terminal protein-tyrosinylation Source: UniProtKB
- microtubule cytoskeleton organization Source: Ensembl
- positive regulation of mitotic cell cycle Source: UniProtKB
- regulation of axon extension Source: Ensembl
- regulation of metaphase plate congression Source: UniProtKB
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Magnesium, Nucleotide-binding, Potassium |
Enzyme and pathway databases
BRENDAi | 6.3.2.25, 2681 |
PathwayCommonsi | Q8NG68 |
Reactomei | R-HSA-8955332, Carboxyterminal post-translational modifications of tubulin |
SIGNORi | Q8NG68 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TTL |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000114999.7 |
HGNCi | HGNC:21586, TTL |
MIMi | 608291, gene |
neXtProti | NX_Q8NG68 |
Subcellular locationi
Cytoskeleton
- spindle microtubule Source: UniProtKB
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 150465 |
OpenTargetsi | ENSG00000114999 |
PharmGKBi | PA134934330 |
Miscellaneous databases
Pharosi | Q8NG68, Tchem |
Chemistry databases
ChEMBLi | CHEMBL5549 |
Polymorphism and mutation databases
BioMutai | TTL |
DMDMi | 47117358 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212434 | 1 – 377 | Tubulin--tyrosine ligaseAdd BLAST | 377 |
Proteomic databases
EPDi | Q8NG68 |
jPOSTi | Q8NG68 |
MassIVEi | Q8NG68 |
MaxQBi | Q8NG68 |
PaxDbi | Q8NG68 |
PeptideAtlasi | Q8NG68 |
PRIDEi | Q8NG68 |
ProteomicsDBi | 73442 |
PTM databases
iPTMneti | Q8NG68 |
PhosphoSitePlusi | Q8NG68 |
Expressioni
Gene expression databases
Bgeei | ENSG00000114999, Expressed in dorsal root ganglion and 217 other tissues |
Genevisiblei | Q8NG68, HS |
Organism-specific databases
HPAi | ENSG00000114999, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 127297, 7 interactors |
IntActi | Q8NG68, 5 interactors |
STRINGi | 9606.ENSP00000233336 |
Chemistry databases
BindingDBi | Q8NG68 |
Miscellaneous databases
RNActi | Q8NG68, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 370 | TTLPROSITE-ProRule annotationAdd BLAST | 368 |
Sequence similaritiesi
Belongs to the tubulin--tyrosine ligase family.Curated
Phylogenomic databases
eggNOGi | KOG2157, Eukaryota |
GeneTreei | ENSGT00940000161907 |
HOGENOMi | CLU_010131_2_0_1 |
InParanoidi | Q8NG68 |
OMAi | DDNSTVY |
OrthoDBi | 626048at2759 |
PhylomeDBi | Q8NG68 |
TreeFami | TF350555 |
Family and domain databases
InterProi | View protein in InterPro IPR004344, TTL/TTLL_fam |
Pfami | View protein in Pfam PF03133, TTL, 1 hit |
PROSITEi | View protein in PROSITE PS51221, TTL, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8NG68-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYTFVVRDEN SSVYAEVSRL LLATGHWKRL RRDNPRFNLM LGERNRLPFG
60 70 80 90 100
RLGHEPGLVQ LVNYYRGADK LCRKASLVKL IKTSPELAES CTWFPESYVI
110 120 130 140 150
YPTNLKTPVA PAQNGIQPPI SNSRTDEREF FLASYNRKKE DGEGNVWIAK
160 170 180 190 200
SSAGAKGEGI LISSEASELL DFIDNQGQVH VIQKYLEHPL LLEPGHRKFD
210 220 230 240 250
IRSWVLVDHQ YNIYLYREGV LRTASEPYHV DNFQDKTCHL TNHCIQKEYS
260 270 280 290 300
KNYGKYEEGN EMFFKEFNQY LTSALNITLE SSILLQIKHI IRNCLLSVEP
310 320 330 340 350
AISTKHLPYQ SFQLFGFDFM VDEELKVWLI EVNGAPACAQ KLYAELCQGI
360 370
VDIAISSVFP PPDVEQPQTQ PAAFIKL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 245 | I → V in CAD98091 (PubMed:17974005).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB071393 mRNA Translation: BAC06832.2 AC012442 Genomic DNA Translation: AAX81998.1 CH471217 Genomic DNA Translation: EAW73589.1 BC036819 mRNA Translation: AAH36819.1 BX538316 mRNA Translation: CAD98091.1 |
CCDSi | CCDS2096.1 |
RefSeqi | NP_714923.1, NM_153712.4 |
Genome annotation databases
Ensembli | ENST00000233336; ENSP00000233336; ENSG00000114999 |
GeneIDi | 150465 |
KEGGi | hsa:150465 |
UCSCi | uc002thu.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB071393 mRNA Translation: BAC06832.2 AC012442 Genomic DNA Translation: AAX81998.1 CH471217 Genomic DNA Translation: EAW73589.1 BC036819 mRNA Translation: AAH36819.1 BX538316 mRNA Translation: CAD98091.1 |
CCDSi | CCDS2096.1 |
RefSeqi | NP_714923.1, NM_153712.4 |
3D structure databases
SMRi | Q8NG68 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 127297, 7 interactors |
IntActi | Q8NG68, 5 interactors |
STRINGi | 9606.ENSP00000233336 |
Chemistry databases
BindingDBi | Q8NG68 |
ChEMBLi | CHEMBL5549 |
PTM databases
iPTMneti | Q8NG68 |
PhosphoSitePlusi | Q8NG68 |
Polymorphism and mutation databases
BioMutai | TTL |
DMDMi | 47117358 |
Proteomic databases
EPDi | Q8NG68 |
jPOSTi | Q8NG68 |
MassIVEi | Q8NG68 |
MaxQBi | Q8NG68 |
PaxDbi | Q8NG68 |
PeptideAtlasi | Q8NG68 |
PRIDEi | Q8NG68 |
ProteomicsDBi | 73442 |
Protocols and materials databases
Antibodypediai | 33272, 161 antibodies |
DNASUi | 150465 |
Genome annotation databases
Ensembli | ENST00000233336; ENSP00000233336; ENSG00000114999 |
GeneIDi | 150465 |
KEGGi | hsa:150465 |
UCSCi | uc002thu.4, human |
Organism-specific databases
CTDi | 150465 |
DisGeNETi | 150465 |
EuPathDBi | HostDB:ENSG00000114999.7 |
GeneCardsi | TTL |
HGNCi | HGNC:21586, TTL |
HPAi | ENSG00000114999, Low tissue specificity |
MIMi | 608291, gene |
neXtProti | NX_Q8NG68 |
OpenTargetsi | ENSG00000114999 |
PharmGKBi | PA134934330 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2157, Eukaryota |
GeneTreei | ENSGT00940000161907 |
HOGENOMi | CLU_010131_2_0_1 |
InParanoidi | Q8NG68 |
OMAi | DDNSTVY |
OrthoDBi | 626048at2759 |
PhylomeDBi | Q8NG68 |
TreeFami | TF350555 |
Enzyme and pathway databases
BRENDAi | 6.3.2.25, 2681 |
PathwayCommonsi | Q8NG68 |
Reactomei | R-HSA-8955332, Carboxyterminal post-translational modifications of tubulin |
SIGNORi | Q8NG68 |
Miscellaneous databases
BioGRID-ORCSi | 150465, 6 hits in 846 CRISPR screens |
ChiTaRSi | TTL, human |
GenomeRNAii | 150465 |
Pharosi | Q8NG68, Tchem |
PROi | PR:Q8NG68 |
RNActi | Q8NG68, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000114999, Expressed in dorsal root ganglion and 217 other tissues |
Genevisiblei | Q8NG68, HS |
Family and domain databases
InterProi | View protein in InterPro IPR004344, TTL/TTLL_fam |
Pfami | View protein in Pfam PF03133, TTL, 1 hit |
PROSITEi | View protein in PROSITE PS51221, TTL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TTL_HUMAN | |
Accessioni | Q8NG68Primary (citable) accession number: Q8NG68 Secondary accession number(s): Q585T3, Q7Z302, Q8N426 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | March 1, 2004 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM