UniProtKB - Q8NG27 (PJA1_HUMAN)
Protein
E3 ubiquitin-protein ligase Praja-1
Gene
PJA1
Organism
Homo sapiens (Human)
Status
Functioni
Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting.By similarity1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 595 – 636 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: Ensembl
GO - Biological processi
- protein catabolic process Source: Ensembl
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8NG27 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SIGNORi | Q8NG27 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase Praja-1 (EC:2.3.2.27)Short name: Praja1 Alternative name(s): RING finger protein 70 RING-type E3 ubiquitin transferase Praja-1Curated |
Gene namesi | Name:PJA1 Synonyms:RNF70 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16648, PJA1 |
MIMi | 300420, gene |
neXtProti | NX_Q8NG27 |
VEuPathDBi | HostDB:ENSG00000181191.11 |
Subcellular locationi
Other locations
- cytoplasm Source: Ensembl
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 64219 |
OpenTargetsi | ENSG00000181191 |
PharmGKBi | PA33342 |
Miscellaneous databases
Pharosi | Q8NG27, Tbio |
Genetic variation databases
BioMutai | PJA1 |
DMDMi | 31076980 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055999 | 1 – 643 | E3 ubiquitin-protein ligase Praja-1Add BLAST | 643 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 265 | PhosphoserineCombined sources | 1 | |
Modified residuei | 277 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 365 | PhosphoserineBy similarity | 1 | |
Modified residuei | 367 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Substrate for E2-dependent ubiquitination.
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q8NG27 |
MassIVEi | Q8NG27 |
MaxQBi | Q8NG27 |
PaxDbi | Q8NG27 |
PeptideAtlasi | Q8NG27 |
PRIDEi | Q8NG27 |
ProteomicsDBi | 246 73411 [Q8NG27-1] 73412 [Q8NG27-2] |
PTM databases
iPTMneti | Q8NG27 |
PhosphoSitePlusi | Q8NG27 |
Expressioni
Tissue specificityi
Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex.1 Publication
Gene expression databases
Bgeei | ENSG00000181191, Expressed in cortical plate and 225 other tissues |
ExpressionAtlasi | Q8NG27, baseline and differential |
Genevisiblei | Q8NG27, HS |
Organism-specific databases
HPAi | ENSG00000181191, Tissue enhanced (epididymis) |
Interactioni
Subunit structurei
Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2.
1 PublicationBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 122109, 101 interactors |
IntActi | Q8NG27, 25 interactors |
STRINGi | 9606.ENSP00000355014 |
Miscellaneous databases
RNActi | Q8NG27, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q8NG27 |
SMRi | Q8NG27 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 276 – 282 | Poly-Asp | 7 | |
Compositional biasi | 340 – 343 | Poly-Arg | 4 |
Domaini
The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 595 – 636 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000154585 |
HOGENOMi | CLU_026830_1_0_1 |
InParanoidi | Q8NG27 |
OMAi | SSKPVWP |
OrthoDBi | 1249953at2759 |
PhylomeDBi | Q8NG27 |
TreeFami | TF330711 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR031227, PJA1 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR15710:SF2, PTHR15710:SF2, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NG27-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGQESSKPVW PNPTGGYQSN TGRRYGRRHA YVSFRPPTSQ RERIASQRKT
60 70 80 90 100
NSEVPMHRSA PSQTTKRSRS PFSTTRRSWD DSESSGTNLN IDNEDYSRYP
110 120 130 140 150
PREYRASGSR RGMAYGHIDS YGADDSEEEG AGPVERPPVR GKTGKFKDDK
160 170 180 190 200
LYDPEKGARS LAGPPPHFSS FSRDVREERD KLDPVPAARC SASRADFLPQ
210 220 230 240 250
SSVASQSSSE GKLATKGDSS ERERREQNLP ARPSRAPVSI CGGGENTSKS
260 270 280 290 300
AEEPVVRPKI RNLASPNCVK PKIFFDTDDD DDMPHSTSRW RDTANDNEGH
310 320 330 340 350
SDGLARRGRG ESSSGYPEPK YPEDKREARS DQVKPEKVPR RRRTMADPDF
360 370 380 390 400
WTHSDDYYKY CDEDSDSDKE WIAALRRKYR SREQTLSSSG ESWETLPGKE
410 420 430 440 450
EREPPQAKVS ASTGTSPGPG ASASAGAGAG ASAGSNGSNY LEEVREPSLQ
460 470 480 490 500
EEQASLEEGE IPWLQYHEND SSSEGDNDSG HELMQPGVFM LDGNNNLEDD
510 520 530 540 550
SSVSEDLEVD WSLFDGFADG LGVAEAISYV DPQFLTYMAL EERLAQAMET
560 570 580 590 600
ALAHLESLAV DVEVANPPAS KESIDALPEI LVTEDHGAVG QEMCCPICCS
610 620 630 640
EYVKGEVATE LPCHHYFHKP CVSIWLQKSG TCPVCRCMFP PPL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EPI8 | K7EPI8_HUMAN | E3 ubiquitin-protein ligase Praja-1 | PJA1 | 144 | Annotation score: | ||
K7EPW5 | K7EPW5_HUMAN | E3 ubiquitin-protein ligase Praja-1 | PJA1 | 67 | Annotation score: |
Sequence cautioni
The sequence BAB13928 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052088 | 432 | S → N1 PublicationCorresponds to variant dbSNP:rs5937160Ensembl. | 1 | |
Natural variantiVAR_052089 | 606 | E → D1 PublicationCorresponds to variant dbSNP:rs11539157Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046995 | 1 – 55 | Missing in isoform 3. CuratedAdd BLAST | 55 | |
Alternative sequenceiVSP_007518 | 98 – 285 | Missing in isoform 2. 1 PublicationAdd BLAST | 188 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF262024 mRNA Translation: AAM53039.1 AF264620 mRNA Translation: AAM53040.1 AL157699 Genomic DNA No translation available. CH471132 Genomic DNA Translation: EAX05367.1 BC048323 mRNA Translation: AAH48323.1 BC075803 mRNA Translation: AAH75803.1 BC105051 mRNA Translation: AAI05052.1 BC105053 mRNA Translation: AAI05054.1 AK021892 mRNA Translation: BAB13928.1 Different initiation. |
CCDSi | CCDS14392.1 [Q8NG27-2] CCDS14393.1 [Q8NG27-1] CCDS35316.1 [Q8NG27-3] |
RefSeqi | NP_001027568.1, NM_001032396.2 [Q8NG27-3] NP_071763.2, NM_022368.4 [Q8NG27-2] NP_660095.1, NM_145119.3 [Q8NG27-1] XP_005262349.1, XM_005262292.1 XP_011529313.1, XM_011531011.2 |
Genome annotation databases
Ensembli | ENST00000361478; ENSP00000355014; ENSG00000181191 [Q8NG27-1] ENST00000374571; ENSP00000363699; ENSG00000181191 [Q8NG27-3] ENST00000374583; ENSP00000363711; ENSG00000181191 [Q8NG27-1] ENST00000374584; ENSP00000363712; ENSG00000181191 [Q8NG27-2] |
GeneIDi | 64219 |
KEGGi | hsa:64219 |
UCSCi | uc004dxg.4, human [Q8NG27-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF262024 mRNA Translation: AAM53039.1 AF264620 mRNA Translation: AAM53040.1 AL157699 Genomic DNA No translation available. CH471132 Genomic DNA Translation: EAX05367.1 BC048323 mRNA Translation: AAH48323.1 BC075803 mRNA Translation: AAH75803.1 BC105051 mRNA Translation: AAI05052.1 BC105053 mRNA Translation: AAI05054.1 AK021892 mRNA Translation: BAB13928.1 Different initiation. |
CCDSi | CCDS14392.1 [Q8NG27-2] CCDS14393.1 [Q8NG27-1] CCDS35316.1 [Q8NG27-3] |
RefSeqi | NP_001027568.1, NM_001032396.2 [Q8NG27-3] NP_071763.2, NM_022368.4 [Q8NG27-2] NP_660095.1, NM_145119.3 [Q8NG27-1] XP_005262349.1, XM_005262292.1 XP_011529313.1, XM_011531011.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2L0B | NMR | - | A | 564-643 | [»] | |
BMRBi | Q8NG27 | |||||
SMRi | Q8NG27 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 122109, 101 interactors |
IntActi | Q8NG27, 25 interactors |
STRINGi | 9606.ENSP00000355014 |
PTM databases
iPTMneti | Q8NG27 |
PhosphoSitePlusi | Q8NG27 |
Genetic variation databases
BioMutai | PJA1 |
DMDMi | 31076980 |
Proteomic databases
jPOSTi | Q8NG27 |
MassIVEi | Q8NG27 |
MaxQBi | Q8NG27 |
PaxDbi | Q8NG27 |
PeptideAtlasi | Q8NG27 |
PRIDEi | Q8NG27 |
ProteomicsDBi | 246 73411 [Q8NG27-1] 73412 [Q8NG27-2] |
Protocols and materials databases
Antibodypediai | 402, 156 antibodies |
DNASUi | 64219 |
Genome annotation databases
Ensembli | ENST00000361478; ENSP00000355014; ENSG00000181191 [Q8NG27-1] ENST00000374571; ENSP00000363699; ENSG00000181191 [Q8NG27-3] ENST00000374583; ENSP00000363711; ENSG00000181191 [Q8NG27-1] ENST00000374584; ENSP00000363712; ENSG00000181191 [Q8NG27-2] |
GeneIDi | 64219 |
KEGGi | hsa:64219 |
UCSCi | uc004dxg.4, human [Q8NG27-1] |
Organism-specific databases
CTDi | 64219 |
DisGeNETi | 64219 |
GeneCardsi | PJA1 |
HGNCi | HGNC:16648, PJA1 |
HPAi | ENSG00000181191, Tissue enhanced (epididymis) |
MIMi | 300420, gene |
neXtProti | NX_Q8NG27 |
OpenTargetsi | ENSG00000181191 |
PharmGKBi | PA33342 |
VEuPathDBi | HostDB:ENSG00000181191.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000154585 |
HOGENOMi | CLU_026830_1_0_1 |
InParanoidi | Q8NG27 |
OMAi | SSKPVWP |
OrthoDBi | 1249953at2759 |
PhylomeDBi | Q8NG27 |
TreeFami | TF330711 |
Enzyme and pathway databases
PathwayCommonsi | Q8NG27 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SIGNORi | Q8NG27 |
Miscellaneous databases
BioGRID-ORCSi | 64219, 4 hits in 501 CRISPR screens |
GeneWikii | PJA1 |
GenomeRNAii | 64219 |
Pharosi | Q8NG27, Tbio |
PROi | PR:Q8NG27 |
RNActi | Q8NG27, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000181191, Expressed in cortical plate and 225 other tissues |
ExpressionAtlasi | Q8NG27, baseline and differential |
Genevisiblei | Q8NG27, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR031227, PJA1 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR15710:SF2, PTHR15710:SF2, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PJA1_HUMAN | |
Accessioni | Q8NG27Primary (citable) accession number: Q8NG27 Secondary accession number(s): A2A322 Q9HAC1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 23, 2003 |
Last sequence update: | May 23, 2003 | |
Last modified: | February 10, 2021 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references