UniProtKB - Q8NFT6 (DBF4B_HUMAN)
Protein
Protein DBF4 homolog B
Gene
DBF4B
Organism
Homo sapiens (Human)
Status
Functioni
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 294 – 343 | DBF4-typePROSITE-ProRule annotationAdd BLAST | 50 |
GO - Molecular functioni
- nucleic acid binding Source: InterPro
- protein kinase activator activity Source: UniProtKB
- protein kinase binding Source: UniProtKB
- protein serine/threonine kinase activator activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- positive regulation of cell population proliferation Source: UniProtKB
- positive regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
- positive regulation of nuclear cell cycle DNA replication Source: UniProtKB
- regulation of cell cycle phase transition Source: GO_Central
Keywordsi
Biological process | Cell cycle |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8NFT6 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein DBF4 homolog BAlternative name(s): Activator of S phase kinase-like protein 1 Short name: ASK-like protein 1 Chiffon homolog B Dbf4-related factor 1 |
Gene namesi | Name:DBF4B Synonyms:ASKL1, DRF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17883, DBF4B |
MIMi | 611661, gene |
neXtProti | NX_Q8NFT6 |
VEuPathDBi | HostDB:ENSG00000161692.17 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Note: Predominantly found in soluble fraction but not in the chromatin-bound fraction.
Nucleus
- nucleoplasm Source: UniProtKB
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- Dbf4-dependent protein kinase complex Source: GO_Central
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 80174 |
OpenTargetsi | ENSG00000161692 |
PharmGKBi | PA143485447 |
Miscellaneous databases
Pharosi | Q8NFT6, Tdark |
Genetic variation databases
BioMutai | DBF4B |
DMDMi | 74715595 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000317553 | 1 – 615 | Protein DBF4 homolog BAdd BLAST | 615 |
Post-translational modificationi
Phosphorylated.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8NFT6 |
jPOSTi | Q8NFT6 |
MassIVEi | Q8NFT6 |
MaxQBi | Q8NFT6 |
PaxDbi | Q8NFT6 |
PeptideAtlasi | Q8NFT6 |
PRIDEi | Q8NFT6 |
ProteomicsDBi | 73350 [Q8NFT6-1] 73351 [Q8NFT6-2] 73352 [Q8NFT6-3] 73353 [Q8NFT6-4] |
PTM databases
iPTMneti | Q8NFT6 |
PhosphoSitePlusi | Q8NFT6 |
Expressioni
Tissue specificityi
Widely expressed. Highly expressed in testis.1 Publication
Developmental stagei
Increases as cells enter in S phase through G2/M phase. The protein has a short half-life (at protein level).2 Publications
Gene expression databases
Bgeei | ENSG00000161692, Expressed in right testis and 140 other tissues |
ExpressionAtlasi | Q8NFT6, baseline and differential |
Genevisiblei | Q8NFT6, HS |
Organism-specific databases
HPAi | ENSG00000161692, Low tissue specificity |
Interactioni
Subunit structurei
Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or DBF4B. Such complexes are stable upon replication stress.
Binary interactionsi
Hide detailsQ8NFT6
With | #Exp. | IntAct |
---|---|---|
LMO2 [P25791] | 3 | EBI-749662,EBI-739696 |
Isoform 2 [Q8NFT6-2]
GO - Molecular functioni
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 123157, 27 interactors |
IntActi | Q8NFT6, 29 interactors |
MINTi | Q8NFT6 |
STRINGi | 9606.ENSP00000323663 |
Miscellaneous databases
RNActi | Q8NFT6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 43 – 133 | BRCTAdd BLAST | 91 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 294 – 343 | DBF4-typePROSITE-ProRule annotationAdd BLAST | 50 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4139, Eukaryota |
GeneTreei | ENSGT00530000063909 |
HOGENOMi | CLU_030726_1_1_1 |
InParanoidi | Q8NFT6 |
OMAi | ASHTCHR |
OrthoDBi | 615385at2759 |
PhylomeDBi | Q8NFT6 |
TreeFami | TF332790 |
Family and domain databases
Gene3Di | 3.30.160.680, 1 hit |
InterProi | View protein in InterPro IPR006572, Znf_DBF IPR038545, Znf_DBF_sf |
Pfami | View protein in Pfam PF07535, zf-DBF, 1 hit |
SMARTi | View protein in SMART SM00586, ZnF_DBF, 1 hit |
PROSITEi | View protein in PROSITE PS51265, ZF_DBF4, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NFT6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSEPGKGDDC LELESSMAES RLRAPDLGVS RCLGKCQKNS PGARKHPFSG
60 70 80 90 100
KSFYLDLPAG KNLQFLTGAI QQLGGVIEGF LSKEVSYIVS SRREVKAESS
110 120 130 140 150
GKSHRGCPSP SPSEVRVETS AMVDPKGSHP RPSRKPVDSV PLSRGKELLQ
160 170 180 190 200
KAIRNQGSIS GGGSGGSSSL LTNARSWGVR ILHVDEMMMH VQQLSLASLC
210 220 230 240 250
VKKQQPKKPE GTCPAAESRT RKVARLKAPF LKIEDESRKF RPFHHQFKSF
260 270 280 290 300
PEISFLGPKD ASPFEAPTTL GSMHHTRESK DGEPSPRSAA HTMPRRKKGY
310 320 330 340 350
CECCQEAFEE LHVHLQSAQH RSFALEAHLY AEVDRIIAQL SHSFADIPFQ
360 370 380 390 400
AGLPRWSGSP ASDCDPLCPE TLHPHQPSHP RAASPRIRKE DSCQASVTQG
410 420 430 440 450
RAAGQQRWTE SLDGVMGPPA SHTCVSATTL LPALPKGSRE QGCLCPCPAS
460 470 480 490 500
FTQSHLVTSL ALLPGEWSPA EDMPLHPSQE NSFAPADIPV KGPLLFPEAR
510 520 530 540 550
PWLMSARCWV RPFPFVTWGC LIPHDTTPLH EEVSPCPCLR LGYLYLLLTQ
560 570 580 590 600
SLWCRVRVPS LSTAGPIPRT SHPCTLAFPS YLNDHDLGHL CQAKPQGWNT
610
PQPFLHCGFL AVDSG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB4E099 | B4E099_HUMAN | Protein DBF4 homolog B | DBF4B | 157 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 207 | K → R in AAL75985 (Ref. 2) Curated | 1 | |
Sequence conflicti | 352 | G → D in AAL75985 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_031014 | 1 – 186 | Missing in isoform 3. 1 PublicationAdd BLAST | 186 | |
Alternative sequenceiVSP_031015 | 157 – 170 | GSISG…GSSSL → VSWGKMGQSRWSPA in isoform 4. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_031016 | 171 – 615 | Missing in isoform 4. 1 PublicationAdd BLAST | 445 | |
Alternative sequenceiVSP_031017 | 397 – 615 | VTQGR…AVDSG → GIPEQDGTVDSTQAPAERAG TGEVPGPIASCQDLGVSVDV FVDPPGIPVSRSPACQCLLP SSGFMELSSGPDLALFGHKR KVQFPSGSAKKRVGASWPQA SFFVPIAPNPCGTRTTSGKR LPSLPLTGHESRLLASLQPL CHSQTCLSLPDPFPWQPTDR PAEFWATQPSWLGKGWPPGP EDSECTATGPVSQEAGQLLS CPTAPGWPSAPLYSATSVQP SGAPVESRSTSLLQPLPASA GASCSRCLWAPQPLQVPCLP VSQPWSQPQPQPQPHAGREL LLRVPKVLGSSQGQAAPD in isoform 3. 1 PublicationAdd BLAST | 219 | |
Alternative sequenceiVSP_031018 | 425 – 431 | VSATTLL → HRPCRLP in isoform 2. 1 Publication | 7 | |
Alternative sequenceiVSP_031019 | 432 – 615 | Missing in isoform 2. 1 PublicationAdd BLAST | 184 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF448801 mRNA Translation: AAM73808.1 AF465820 mRNA Translation: AAL75985.1 AK023149 mRNA Translation: BAB14431.1 CH471178 Genomic DNA Translation: EAW51581.1 CH471178 Genomic DNA Translation: EAW51584.1 CH471178 Genomic DNA Translation: EAW51587.1 CH471178 Genomic DNA Translation: EAW51582.1 CH471178 Genomic DNA Translation: EAW51586.1 BC016158 mRNA Translation: AAH16158.1 |
CCDSi | CCDS11485.1 [Q8NFT6-1] CCDS45706.2 [Q8NFT6-2] |
RefSeqi | NP_079380.2, NM_025104.3 [Q8NFT6-2] NP_663696.1, NM_145663.2 [Q8NFT6-1] XP_006722165.1, XM_006722102.3 [Q8NFT6-3] XP_011523583.1, XM_011525281.2 [Q8NFT6-3] |
Genome annotation databases
Ensembli | ENST00000315005; ENSP00000323663; ENSG00000161692 [Q8NFT6-1] ENST00000393547; ENSP00000377178; ENSG00000161692 [Q8NFT6-2] |
GeneIDi | 80174 |
KEGGi | hsa:80174 |
UCSCi | uc002ihf.4, human [Q8NFT6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF448801 mRNA Translation: AAM73808.1 AF465820 mRNA Translation: AAL75985.1 AK023149 mRNA Translation: BAB14431.1 CH471178 Genomic DNA Translation: EAW51581.1 CH471178 Genomic DNA Translation: EAW51584.1 CH471178 Genomic DNA Translation: EAW51587.1 CH471178 Genomic DNA Translation: EAW51582.1 CH471178 Genomic DNA Translation: EAW51586.1 BC016158 mRNA Translation: AAH16158.1 |
CCDSi | CCDS11485.1 [Q8NFT6-1] CCDS45706.2 [Q8NFT6-2] |
RefSeqi | NP_079380.2, NM_025104.3 [Q8NFT6-2] NP_663696.1, NM_145663.2 [Q8NFT6-1] XP_006722165.1, XM_006722102.3 [Q8NFT6-3] XP_011523583.1, XM_011525281.2 [Q8NFT6-3] |
3D structure databases
SMRi | Q8NFT6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 123157, 27 interactors |
IntActi | Q8NFT6, 29 interactors |
MINTi | Q8NFT6 |
STRINGi | 9606.ENSP00000323663 |
PTM databases
iPTMneti | Q8NFT6 |
PhosphoSitePlusi | Q8NFT6 |
Genetic variation databases
BioMutai | DBF4B |
DMDMi | 74715595 |
Proteomic databases
EPDi | Q8NFT6 |
jPOSTi | Q8NFT6 |
MassIVEi | Q8NFT6 |
MaxQBi | Q8NFT6 |
PaxDbi | Q8NFT6 |
PeptideAtlasi | Q8NFT6 |
PRIDEi | Q8NFT6 |
ProteomicsDBi | 73350 [Q8NFT6-1] 73351 [Q8NFT6-2] 73352 [Q8NFT6-3] 73353 [Q8NFT6-4] |
Protocols and materials databases
Antibodypediai | 29863, 137 antibodies |
Genome annotation databases
Ensembli | ENST00000315005; ENSP00000323663; ENSG00000161692 [Q8NFT6-1] ENST00000393547; ENSP00000377178; ENSG00000161692 [Q8NFT6-2] |
GeneIDi | 80174 |
KEGGi | hsa:80174 |
UCSCi | uc002ihf.4, human [Q8NFT6-1] |
Organism-specific databases
CTDi | 80174 |
DisGeNETi | 80174 |
GeneCardsi | DBF4B |
HGNCi | HGNC:17883, DBF4B |
HPAi | ENSG00000161692, Low tissue specificity |
MIMi | 611661, gene |
neXtProti | NX_Q8NFT6 |
OpenTargetsi | ENSG00000161692 |
PharmGKBi | PA143485447 |
VEuPathDBi | HostDB:ENSG00000161692.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4139, Eukaryota |
GeneTreei | ENSGT00530000063909 |
HOGENOMi | CLU_030726_1_1_1 |
InParanoidi | Q8NFT6 |
OMAi | ASHTCHR |
OrthoDBi | 615385at2759 |
PhylomeDBi | Q8NFT6 |
TreeFami | TF332790 |
Enzyme and pathway databases
PathwayCommonsi | Q8NFT6 |
Miscellaneous databases
BioGRID-ORCSi | 80174, 7 hits in 992 CRISPR screens |
ChiTaRSi | DBF4B, human |
GenomeRNAii | 80174 |
Pharosi | Q8NFT6, Tdark |
PROi | PR:Q8NFT6 |
RNActi | Q8NFT6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000161692, Expressed in right testis and 140 other tissues |
ExpressionAtlasi | Q8NFT6, baseline and differential |
Genevisiblei | Q8NFT6, HS |
Family and domain databases
Gene3Di | 3.30.160.680, 1 hit |
InterProi | View protein in InterPro IPR006572, Znf_DBF IPR038545, Znf_DBF_sf |
Pfami | View protein in Pfam PF07535, zf-DBF, 1 hit |
SMARTi | View protein in SMART SM00586, ZnF_DBF, 1 hit |
PROSITEi | View protein in PROSITE PS51265, ZF_DBF4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DBF4B_HUMAN | |
Accessioni | Q8NFT6Primary (citable) accession number: Q8NFT6 Secondary accession number(s): D3DX56 Q9H912 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | October 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot