UniProtKB - Q8NFJ5 (RAI3_HUMAN)
Protein
Retinoic acid-induced protein 3
Gene
GPRC5A
Organism
Homo sapiens (Human)
Status
Functioni
Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling (By similarity). May act as a lung tumor suppressor (PubMed:18000218).By similarity1 Publication
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- G protein-coupled receptor activity Source: ProtInc
- protein kinase activator activity Source: GO_Central
GO - Biological processi
- negative regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
- signal transduction Source: ProtInc
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
PathwayCommonsi | Q8NFJ5 |
Names & Taxonomyi
Protein namesi | Recommended name: Retinoic acid-induced protein 3Alternative name(s): G-protein coupled receptor family C group 5 member A Phorbol ester induced gene 11 Publication Short name: PEIG-1 Retinoic acid-induced gene 1 protein Short name: RAIG-1 |
Gene namesi | Name:GPRC5A Synonyms:GPCR5A, RAI3, RAIG1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9836, GPRC5A |
MIMi | 604138, gene |
neXtProti | NX_Q8NFJ5 |
VEuPathDBi | HostDB:ENSG00000013588.5 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Other locations
- Cytoplasmic vesicle membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Note: Localized in perinuclear vesicles, probably Golgi-associated vesicles.1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleolus Source: HPA
Plasma Membrane
- integral component of plasma membrane Source: ProtInc
- plasma membrane Source: UniProtKB
Other locations
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
- intracellular membrane-bounded organelle Source: HPA
- receptor complex Source: GO_Central
- vesicle Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | ExtracellularCuratedAdd BLAST | 33 | |
Transmembranei | 34 – 54 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 55 – 68 | CytoplasmicCuratedAdd BLAST | 14 | |
Transmembranei | 69 – 89 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 90 – 97 | ExtracellularCurated | 8 | |
Transmembranei | 98 – 118 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 119 – 129 | CytoplasmicCuratedAdd BLAST | 11 | |
Transmembranei | 130 – 150 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 151 – 176 | ExtracellularCuratedAdd BLAST | 26 | |
Transmembranei | 177 – 197 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 198 – 212 | CytoplasmicCuratedAdd BLAST | 15 | |
Transmembranei | 213 – 233 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 234 – 247 | ExtracellularCuratedAdd BLAST | 14 | |
Transmembranei | 248 – 268 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 269 – 357 | CytoplasmicCuratedAdd BLAST | 89 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, MembranePathology & Biotechi
Keywords - Diseasei
Tumor suppressorOrganism-specific databases
DisGeNETi | 9052 |
OpenTargetsi | ENSG00000013588 |
PharmGKBi | PA34194 |
Miscellaneous databases
Pharosi | Q8NFJ5, Tbio |
Chemistry databases
DrugBanki | DB00755, Tretinoin |
Genetic variation databases
BioMutai | GPRC5A |
DMDMi | 46396943 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206895 | 1 – 357 | Retinoic acid-induced protein 3Add BLAST | 357 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 158 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 301 | PhosphoserineCombined sources | 1 | |
Modified residuei | 317 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 320 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 345 | PhosphoserineCombined sources | 1 | |
Modified residuei | 347 | PhosphotyrosineCombined sources1 Publication | 1 | |
Modified residuei | 350 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Phosphorylated in two conserved double-tyrosine motifs, TYR-317/TYR-320 and TYR-347/TYR-350, by EGFR; leading to inactivation of the tumor suppressive function of GPRC5A in lung cancer cells. TYR-317 and TYR-320 are the preferred residues responsible for EGFR-mediated GPRC5A phosphorylation.1 Publication
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q8NFJ5 |
jPOSTi | Q8NFJ5 |
MassIVEi | Q8NFJ5 |
MaxQBi | Q8NFJ5 |
PaxDbi | Q8NFJ5 |
PeptideAtlasi | Q8NFJ5 |
PRIDEi | Q8NFJ5 |
ProteomicsDBi | 73316 |
PTM databases
GlyGeni | Q8NFJ5, 1 site |
iPTMneti | Q8NFJ5 |
PhosphoSitePlusi | Q8NFJ5 |
SwissPalmi | Q8NFJ5 |
Expressioni
Tissue specificityi
Expressed at high level in fetal and adult lung tissues but repressed in most human lung cancers (PubMed:9857033, PubMed:18000218). Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at low to moderate levels. Not detectable in fetal heart, brain, and liver and adult heart, brain, liver, skeletal muscle, pancreas, spleen, thymus, and peripheral leukocytes. According to PubMed:10783259, expressed at low but detectable level in pancreas and heart.4 Publications
Inductioni
By all-trans retinoic acid (ATRA).1 Publication
Gene expression databases
Bgeei | ENSG00000013588, Expressed in amniotic fluid and 188 other tissues |
ExpressionAtlasi | Q8NFJ5, baseline and differential |
Genevisiblei | Q8NFJ5, HS |
Organism-specific databases
HPAi | ENSG00000013588, Tissue enhanced (lung) |
Interactioni
Subunit structurei
Interacts (via its transmembrane domain) with EGFR.
1 PublicationGO - Molecular functioni
- cadherin binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 114514, 51 interactors |
IntActi | Q8NFJ5, 32 interactors |
STRINGi | 9606.ENSP00000014914 |
Miscellaneous databases
RNActi | Q8NFJ5, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 3 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RRIY, Eukaryota |
GeneTreei | ENSGT00950000182961 |
HOGENOMi | CLU_044162_0_0_1 |
InParanoidi | Q8NFJ5 |
OMAi | NDYEGRK |
OrthoDBi | 807909at2759 |
PhylomeDBi | Q8NFJ5 |
TreeFami | TF321410 |
Family and domain databases
InterProi | View protein in InterPro IPR017978, GPCR_3_C |
Pfami | View protein in Pfam PF00003, 7tm_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q8NFJ5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATTVPDGCR NGLKSKYYRL CDKAEAWGIV LETVATAGVV TSVAFMLTLP
60 70 80 90 100
ILVCKVQDSN RRKMLPTQFL FLLGVLGIFG LTFAFIIGLD GSTGPTRFFL
110 120 130 140 150
FGILFSICFS CLLAHAVSLT KLVRGRKPLS LLVILGLAVG FSLVQDVIAI
160 170 180 190 200
EYIVLTMNRT NVNVFSELSA PRRNEDFVLL LTYVLFLMAL TFLMSSFTFC
210 220 230 240 250
GSFTGWKRHG AHIYLTMLLS IAIWVAWITL LMLPDFDRRW DDTILSSALA
260 270 280 290 300
ANGWVFLLAY VSPEFWLLTK QRNPMDYPVE DAFCKPQLVK KSYGVENRAY
310 320 330 340 350
SQEEITQGFE ETGDTLYAPY STHFQLQNQP PQKEFSIPRA HAWPSPYKDY
EVKKEGS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5GWG3 | F5GWG3_HUMAN | Retinoic acid-induced protein 3 | GPRC5A | 273 | Annotation score: | ||
A0A3B3ITN8 | A0A3B3ITN8_HUMAN | Retinoic acid-induced protein 3 | GPRC5A | 339 | Annotation score: | ||
H0YFN2 | H0YFN2_HUMAN | Retinoic acid-induced protein 3 | GPRC5A | 66 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 292 | S → G in AAM77594 (PubMed:8832110).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049281 | 9 | C → F. Corresponds to variant dbSNP:rs11550683Ensembl. | 1 | |
Natural variantiVAR_018296 | 118 | S → G. Corresponds to variant dbSNP:rs850932Ensembl. | 1 | |
Natural variantiVAR_049282 | 182 | T → A. Corresponds to variant dbSNP:rs12368599Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF506289 mRNA Translation: AAM77594.1 AF095448 mRNA Translation: AAC98506.1 AK001761 mRNA Translation: BAA91890.1 AK122672 mRNA Translation: BAG53657.1 CH471094 Genomic DNA Translation: EAW96289.1 BC003665 mRNA Translation: AAH03665.1 |
CCDSi | CCDS8657.1 |
RefSeqi | NP_003970.1, NM_003979.3 |
Genome annotation databases
Ensembli | ENST00000014914; ENSP00000014914; ENSG00000013588 |
GeneIDi | 9052 |
KEGGi | hsa:9052 |
UCSCi | uc001rba.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF506289 mRNA Translation: AAM77594.1 AF095448 mRNA Translation: AAC98506.1 AK001761 mRNA Translation: BAA91890.1 AK122672 mRNA Translation: BAG53657.1 CH471094 Genomic DNA Translation: EAW96289.1 BC003665 mRNA Translation: AAH03665.1 |
CCDSi | CCDS8657.1 |
RefSeqi | NP_003970.1, NM_003979.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 114514, 51 interactors |
IntActi | Q8NFJ5, 32 interactors |
STRINGi | 9606.ENSP00000014914 |
Chemistry databases
DrugBanki | DB00755, Tretinoin |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q8NFJ5, 1 site |
iPTMneti | Q8NFJ5 |
PhosphoSitePlusi | Q8NFJ5 |
SwissPalmi | Q8NFJ5 |
Genetic variation databases
BioMutai | GPRC5A |
DMDMi | 46396943 |
Proteomic databases
EPDi | Q8NFJ5 |
jPOSTi | Q8NFJ5 |
MassIVEi | Q8NFJ5 |
MaxQBi | Q8NFJ5 |
PaxDbi | Q8NFJ5 |
PeptideAtlasi | Q8NFJ5 |
PRIDEi | Q8NFJ5 |
ProteomicsDBi | 73316 |
Protocols and materials databases
Antibodypediai | 1914, 400 antibodies |
DNASUi | 9052 |
Genome annotation databases
Ensembli | ENST00000014914; ENSP00000014914; ENSG00000013588 |
GeneIDi | 9052 |
KEGGi | hsa:9052 |
UCSCi | uc001rba.4, human |
Organism-specific databases
CTDi | 9052 |
DisGeNETi | 9052 |
GeneCardsi | GPRC5A |
HGNCi | HGNC:9836, GPRC5A |
HPAi | ENSG00000013588, Tissue enhanced (lung) |
MIMi | 604138, gene |
neXtProti | NX_Q8NFJ5 |
OpenTargetsi | ENSG00000013588 |
PharmGKBi | PA34194 |
VEuPathDBi | HostDB:ENSG00000013588.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502RRIY, Eukaryota |
GeneTreei | ENSGT00950000182961 |
HOGENOMi | CLU_044162_0_0_1 |
InParanoidi | Q8NFJ5 |
OMAi | NDYEGRK |
OrthoDBi | 807909at2759 |
PhylomeDBi | Q8NFJ5 |
TreeFami | TF321410 |
Enzyme and pathway databases
PathwayCommonsi | Q8NFJ5 |
Miscellaneous databases
BioGRID-ORCSi | 9052, 6 hits in 874 CRISPR screens |
ChiTaRSi | GPRC5A, human |
GeneWikii | GPRC5A |
GenomeRNAii | 9052 |
Pharosi | Q8NFJ5, Tbio |
PROi | PR:Q8NFJ5 |
RNActi | Q8NFJ5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000013588, Expressed in amniotic fluid and 188 other tissues |
ExpressionAtlasi | Q8NFJ5, baseline and differential |
Genevisiblei | Q8NFJ5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR017978, GPCR_3_C |
Pfami | View protein in Pfam PF00003, 7tm_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAI3_HUMAN | |
Accessioni | Q8NFJ5Primary (citable) accession number: Q8NFJ5 Secondary accession number(s): B3KV45, O95357 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | April 13, 2004 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families