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Protein

Cytosolic endo-beta-N-acetylglucosaminidase

Gene

ENGASE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.1 Publication

Catalytic activityi

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.96 2681
ReactomeiR-HSA-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle

Protein family/group databases

CAZyiGH85 Glycoside Hydrolase Family 85

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
Short name:
ENGase
Gene namesi
Name:ENGASE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000167280.16
HGNCiHGNC:24622 ENGASE
MIMi611898 gene
neXtProtiNX_Q8NFI3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi64772
OpenTargetsiENSG00000167280
PharmGKBiPA164719123

Chemistry databases

ChEMBLiCHEMBL5172

Polymorphism and mutation databases

BioMutaiENGASE
DMDMi74715557

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003288671 – 743Cytosolic endo-beta-N-acetylglucosaminidaseAdd BLAST743

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei66PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8NFI3
MaxQBiQ8NFI3
PaxDbiQ8NFI3
PeptideAtlasiQ8NFI3
PRIDEiQ8NFI3
ProteomicsDBi73312
73313 [Q8NFI3-2]
73314 [Q8NFI3-3]

PTM databases

iPTMnetiQ8NFI3
PhosphoSitePlusiQ8NFI3

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in thymus and spleen.1 Publication

Gene expression databases

BgeeiENSG00000167280
ExpressionAtlasiQ8NFI3 baseline and differential

Organism-specific databases

HPAiHPA021551

Interactioni

Protein-protein interaction databases

BioGridi122283, 1 interactor
IntActiQ8NFI3, 2 interactors
STRINGi9606.ENSP00000462333

Chemistry databases

BindingDBiQ8NFI3

Structurei

3D structure databases

ProteinModelPortaliQ8NFI3
SMRiQ8NFI3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini291 – 383BRCTPROSITE-ProRule annotationAdd BLAST93

Sequence similaritiesi

Belongs to the glycosyl hydrolase 85 family.Curated

Phylogenomic databases

eggNOGiKOG2331 Eukaryota
COG4724 LUCA
GeneTreeiENSGT00390000018512
HOGENOMiHOG000082678
HOVERGENiHBG107848
InParanoidiQ8NFI3
KOiK01227
OMAiPTKLARW
OrthoDBiEOG091G0A7Z
PhylomeDBiQ8NFI3
TreeFamiTF314391

Family and domain databases

Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR032979 ENGase
IPR008979 Galactose-bd-like_sf
IPR005201 Glyco_hydro_85
PANTHERiPTHR13246 PTHR13246, 1 hit
PfamiView protein in Pfam
PF03644 Glyco_hydro_85, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NFI3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI
60 70 80 90 100
KDEEEETVFR EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL
110 120 130 140 150
EDGFNVALEP LACRQPPLSS QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY
160 170 180 190 200
AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA HRHGVCVLGT FITEWNEGGR
210 220 230 240 250
LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL SLAAVGNMPP
260 270 280 290 300
FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF
310 320 330 340 350
FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL
360 370 380 390 400
IRKHGFSVAL FAPGWVYECL EKKDFFQNQD KFWGRLERYL PTHSICSLPF
410 420 430 440 450
VTSFCLGMGA RRVCYGQEEA VGPWYHLSAQ EIQPLFGEHR LGGDGRGWVR
460 470 480 490 500
THCCLEDAWH GGSSLLVRGV IPPEVGNVAV RLFSLQAPVP PKIYLSMVYK
510 520 530 540 550
LEGPTDVTVA LELTTGDAGS CHIGGISVLN AETSSRHSLR PLRVPPTKLA
560 570 580 590 600
RWVGRCGRQL SGGWVQHCYE VSLRGCLLLD LLVCFSRPPG SREEESFTCR
610 620 630 640 650
LGEIQVVDAA SLLAPLPQVQ AVTISHIRWQ PSASEREGPP ALLQLSCTLH
660 670 680 690 700
WSFLLSQVRC FRIHCWGGMS DDSPGRELPR PEMPMFLGLA FATQYRIVDL
710 720 730 740
LVEAAGPGQD RRMEFLVEPV PKEGFRVPQA EWGRAVLLYS APA
Length:743
Mass (Da):83,987
Last modified:October 1, 2002 - v1
Checksum:i384DA533AE549FC5
GO
Isoform 2 (identifier: Q8NFI3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     364-377: GWVYECLEKKDFFQ → SCSVFPGVGNLLCC
     378-743: Missing.

Note: No experimental confirmation available.
Show »
Length:377
Mass (Da):43,107
Checksum:iA60D0AE20C2EDD79
GO
Isoform 3 (identifier: Q8NFI3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     347-406: SLELIRKHGF...SLPFVTSFCL → VGGGFRPRAS...CPAPAKLCPH
     407-743: Missing.

Note: No experimental confirmation available.
Show »
Length:406
Mass (Da):46,090
Checksum:i4EB91EC292C1A61A
GO

Sequence cautioni

The sequence AAH24213 differs from that shown. Reason: Frameshift at position 694.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152F → L in BAB15158 (PubMed:14702039).Curated1
Sequence conflicti224T → A in CAH56405 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_060188596S → N1 PublicationCorresponds to variant dbSNP:rs4789879Ensembl.1
Natural variantiVAR_060189731E → K. Corresponds to variant dbSNP:rs11871357Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032835347 – 406SLELI…TSFCL → VGGGFRPRASGPVPPLGPHF LMDLPFPSAPQRNDSSCSSQ SGDPVALRNRCPAPAKLCPH in isoform 3. 1 PublicationAdd BLAST60
Alternative sequenceiVSP_032836364 – 377GWVYE…KDFFQ → SCSVFPGVGNLLCC in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_032837378 – 743Missing in isoform 2. 1 PublicationAdd BLAST366
Alternative sequenceiVSP_032838407 – 743Missing in isoform 3. 1 PublicationAdd BLAST337

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF512564 mRNA Translation: AAM80487.1
AK025518 mRNA Translation: BAB15158.1
CH471099 Genomic DNA Translation: EAW89562.1
AL110283 mRNA Translation: CAH56405.1
BC024213 mRNA Translation: AAH24213.2 Frameshift.
CCDSiCCDS42394.1 [Q8NFI3-1]
RefSeqiNP_001036038.1, NM_001042573.2 [Q8NFI3-1]
UniGeneiHs.29288

Genome annotation databases

EnsembliENST00000579016; ENSP00000462333; ENSG00000167280 [Q8NFI3-1]
GeneIDi64772
KEGGihsa:64772
UCSCiuc002jwv.5 human [Q8NFI3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiENASE_HUMAN
AccessioniPrimary (citable) accession number: Q8NFI3
Secondary accession number(s): Q659F0, Q8TB86, Q9H6U4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

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